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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Caracteriza??o em acessos de mel?o do Banco Ativo de Germoplasma de cucurbit?ceas para o Nordeste brasileiro

Amorim, Clisneide Coelho de 19 July 2016 (has links)
Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-02-13T21:42:15Z No. of bitstreams: 1 Disserta??o Clisneide 2016.pdf: 1569914 bytes, checksum: 5fb4b229474a974cde160deeeb5bea49 (MD5) / Made available in DSpace on 2017-02-13T21:42:15Z (GMT). No. of bitstreams: 1 Disserta??o Clisneide 2016.pdf: 1569914 bytes, checksum: 5fb4b229474a974cde160deeeb5bea49 (MD5) Previous issue date: 2016-07-19 / The melon (Cucumis melo L.) production is growing steadily in the last years and it is demanded for the internal as well as the external markets. The melon produced belongs to the botanical group inodorus, yellow type, although the cultivars used present susceptibility to biotic stresses and, therefore, there is a need to search new germplasm in order to give support to melon breeding programs. On the other hand, there are melons cultivated by small farmers in the traditional agriculture of Northeast Brazil and a sample of these melons is stored in the Active Germplasm Bank of Cucurbits for the Northeast Brazil, located in the Semiarid Embrapa, at Petrolina-PE. Thus, the present study aimed at to estimate the genetic variability within and among melon accessions using morphological and molecular markers in order to identify the melon subspecies and their respective botanical groups. In the morphological characterization the parental e S1 generations were used recording quantitative and qualitative descriptors and some accessions were partitioned in subacessios. In the molecular characterization 28 ISSR primers were used. Using the morphological data is was possible to identify the subspecies agrestis with the botanical groupsmakwua and momordica and the subespecies melo with the botanical group cantalupensis. The UPGMA clusters showed great genetic diversity within and among the accessions and subaccessions. There was a reasonable agreement between the clusters based on morphological and molecular data, although the molecular data were more precise in the separation of the clusters. However, there is indication of introgression of traits of different subspecies and their botanical groups in the melon cultivated in the traditional agriculture of Northeast Brazil. / A produ??o do mel?o (Cucumis melo L.) no Brasil vem aumentando significativamente nos ?ltimos anos, sendo apreciado nos mercados internos e externos. O mel?o mais produzido ? do grupo bot?nico inodorus, tipo ?amarelo?, por?m, as cultivares apresentam suscetibilidade a estresses bi?ticos e, portanto, h? necessidade de busca por novo germoplasma para dar suporte aos programas de melhoramento de mel?o. Por outro lado, existem mel?es cultivados por pequenos agricultores e uma boa amostra desses mel?es foi coletada eencontra-se armazenada no BAG de Cucurbit?ceas para o Nordeste brasileiro localizado na Embrapa Semi?rido, Petrolina-PE. Assim, o presente estudo teve como objetivo estudar uma amostra de acessos de mel?o da agricultura tradicional e estimar a variabilidade gen?tica entre e dentro de uma amostra de acessos desses mel?es utilizando descritores morfol?gicos e moleculares, a fim de identificar as subesp?cies e seus respectivos grupos bot?nicos. Na caracteriza??o morfol?gica foram usadasas gera??es parentais e S1 utilizando descritores quantitativos e qualitativos e alguns acessos foram desdobrados em subacessos. Para a caracteriza??o molecular os subacessos foram analisados com 28 primers ISSR. Com os dados morfol?gicos foi poss?vel identificar a subesp?cie agrestis com os grupos bot?nicos makuwa e momordica e a subesp?cie melo com o grupo bot?nico cantalupensis.Os agrupamentos pelo m?todo UPGMA mostraram grande diversidade gen?tica entre e dentro dos acessos e dos subacessos. Houve uma razo?vel concord?ncia entre os dados morfol?gicos e moleculares, embora os dados moleculares foram mais precisos na separa??o dos grupos. Contudo, h? indica??o de que houve introgress?o de caracteres das diferentes subesp?cies e diferentes grupos bot?nicos no material cultivado pelos pequenos agricultores.

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