The crisis of the water resources become a serious problem in recent years. Besides the global warming the problem mostly comes from quick population growth, intense industrial developments and low efficiency agricultural implementations. Biofilters are widely been used to either reduce pollution loads or also as a water conservation tool. And the vertical-flow biofilters act as a kind of bio-filter has gain the advantages of low maintenance, small footprint, greater capacities on both the hydraulic and organic loadings. It often used in to treat aquaculture wastewater for recycling during the filter stage. This study is, therefore, focusing on the bio-treatment processes to recycle the wastewater discharged from Chlorella production. Preserving water resources is one big issue of this study, Reuse the nutrients is another tough objective. For reusing the water with as much nutrients as possible and get the organic content off the water is the major target of the study. This study has been separated in two stages. A preliminary study has first been carried out in order to understand the Chlorella behaviors in more detail. Second phase includes the treatment tests with conventional activated sludge (AS) method and the bio-filters. The results have shown that ammonia is preferred by Chlorella as the nitrogen source. Light plays an important role on the treatment for removing algae activities. Aerobic digestion has shown better efficiency. AS can accept as high as 20% of daily input to the system volume, the system is not capable to bear more. While the biofilters, using either zeolite and LECA as the media, have shown satisfied results. When the hydraulic loading stay between 0.30 ~ 2.09 m3 m-2 day-1 to the system, the SS, COD, Chl-a removal rates can reach 90%, and more than 96% of total inorganic nitrogen (TIN) and 76% phosphorus can be preserved in the recycled water, respectively.