<p> </p><p> </p><p> </p><p>The extinction risk of threatened species has traditionally been assessed by the use of tools of Population Viability Analysis (PVA). Species interactions, however, have seldom been accounted for in PVA:s. The omission of species interactions in risk assessments may further lead to serious mistakes when setting target sizes of populations. Even a slight abundance decrease of a target species may result in changes of the community structure; in the worst case leading to a highly impoverished community. Of critical importance to conservation is therefore the question of how many individuals of a certain population that is needed in order to avoid this kind of consequences. In the current study, a stochastic multispecies model is used to estimate minimum ecological viable populations (MEVP); earlier defined as “the minimum size of a population that can survive before itself or some other species in the community becomes extinct”. The MEVP:s are compared to population sizes given by a single species model where interactions with other species are treated as a constant source incorporated in the species specific growth rate. MEVP:s are found to be larger than the population sizes given by the single species model. The results are trophic level dependent and multispecies approaches are suggested to be of major importance when setting target levels for species at the basal level. Species at higher trophic levels, however, are altogether more prone to extinction than species at the basal level, irrespective of food web size and food web complexity.</p><p> </p>

<p>Transesophageal echocardiography (TEE) has become a useful tool in monitoring the heart in patients during open-heart surgery. This study was undertaken to evaluate whether it is feasible to use TEE to assess left ventricular myocardial viability in anesthetized patients scheduled for coronary artery bypass grafting (CABG).</p><p>A total of 84 patients were studied. To test myocardial viability, TEE and a low-dose dobutamine stress regimen were used. Echocardiographic data were analyzed off-line using a visual or semiautomatic analysis of segmental left ventricular wall motion (LVWM). Visual assessment was performed by readers blinded to the sequence of events. The agreement between readers in visual analysis of segmental LVWM in the transgastric short-axis view was 73% or higher. Segmental LVWM assessed by TEE was compared to hemodynamic data obtained by thermodilution pulmonary artery catheter (PAC) and coronary angiographic data. Also, using the same low-dose dobutamine stress regimen, TEE findings in the anesthetized patient perioperatively were compared with preoperative transthoracic echocardiography (TTE) findings in the awake patient.</p><p>TEE was found to be feasible and adequate for testing left segmental ventricular viability. A concomitant increase in stroke volume assessed by PAC and decrease in LVWM-score assessed by TEE was found with dobutamine stimulation. Abnormal segmental LVWM corresponded to angiographically stenosed supplying coronary artery vessels. During dobutamine stimulation, 69% of the corresponding segments responded which is a sign of viability. The LVWM response to preoperative TTE and perioperative TEE dobutamine stress was comparable except for a significant difference in the apical segments.</p><p>This study showed that perioperative TEE dobutamine stress could be used to test left ventricular viability and was also a valuable supplement to PAC, angiography and TTE. The acquired knowledge is important and suggest that further development of transesophageal ultrasound technology is warranted.</p>

Transesophageal echocardiography (TEE) has become a useful tool in monitoring the heart in patients during open-heart surgery. This study was undertaken to evaluate whether it is feasible to use TEE to assess left ventricular myocardial viability in anesthetized patients scheduled for coronary artery bypass grafting (CABG). A total of 84 patients were studied. To test myocardial viability, TEE and a low-dose dobutamine stress regimen were used. Echocardiographic data were analyzed off-line using a visual or semiautomatic analysis of segmental left ventricular wall motion (LVWM). Visual assessment was performed by readers blinded to the sequence of events. The agreement between readers in visual analysis of segmental LVWM in the transgastric short-axis view was 73% or higher. Segmental LVWM assessed by TEE was compared to hemodynamic data obtained by thermodilution pulmonary artery catheter (PAC) and coronary angiographic data. Also, using the same low-dose dobutamine stress regimen, TEE findings in the anesthetized patient perioperatively were compared with preoperative transthoracic echocardiography (TTE) findings in the awake patient. TEE was found to be feasible and adequate for testing left segmental ventricular viability. A concomitant increase in stroke volume assessed by PAC and decrease in LVWM-score assessed by TEE was found with dobutamine stimulation. Abnormal segmental LVWM corresponded to angiographically stenosed supplying coronary artery vessels. During dobutamine stimulation, 69% of the corresponding segments responded which is a sign of viability. The LVWM response to preoperative TTE and perioperative TEE dobutamine stress was comparable except for a significant difference in the apical segments. This study showed that perioperative TEE dobutamine stress could be used to test left ventricular viability and was also a valuable supplement to PAC, angiography and TTE. The acquired knowledge is important and suggest that further development of transesophageal ultrasound technology is warranted.

The extinction risk of threatened species has traditionally been assessed by the use of tools of Population Viability Analysis (PVA). Species interactions, however, have seldom been accounted for in PVA:s. The omission of species interactions in risk assessments may further lead to serious mistakes when setting target sizes of populations. Even a slight abundance decrease of a target species may result in changes of the community structure; in the worst case leading to a highly impoverished community. Of critical importance to conservation is therefore the question of how many individuals of a certain population that is needed in order to avoid this kind of consequences. In the current study, a stochastic multispecies model is used to estimate minimum ecological viable populations (MEVP); earlier defined as “the minimum size of a population that can survive before itself or some other species in the community becomes extinct”. The MEVP:s are compared to population sizes given by a single species model where interactions with other species are treated as a constant source incorporated in the species specific growth rate. MEVP:s are found to be larger than the population sizes given by the single species model. The results are trophic level dependent and multispecies approaches are suggested to be of major importance when setting target levels for species at the basal level. Species at higher trophic levels, however, are altogether more prone to extinction than species at the basal level, irrespective of food web size and food web complexity.

Materials based on novel Off-Stoichiometry Thiol-Ene polymers, abbreviated OSTE, show promising properties as materials forlow cost and scalable manufacturing of micro- and nanosystems such as lab-on-chip devices. The OSTE materials have tunablemechanical properties, offer possibility for low temperature bonding to many surfaces via tunable surface chemistry, and can beused in soft lithography. Unlike the commonly used elastomer poly(dimethylsiloxane), PDMS, the OSTE materials have lowpermeability for gasses, are resistant to common solvents and can be more permanently surface modified.In this master’s thesis project, the OSTE materials have been evaluated with focus on compatibility with cells, possibility fornanostructuring using soft lithography and the use of OSTE as a flexible support for conducting polymers.Results from cell seeding studies with HEP G2 cells suggest that cells can proliferate on a low thiol off-stoichiometry OSTEmaterial for at least five days. The biocompatibility for this type of OSTE material may be similar to poly(styrene). However, highlevels of free thiol monomers in the material decrease cell viability considerably.By using soft lithography techniques it is possible to fabricate OSTE nanochannels with at least the dimensions of 400 nm x 15nm. Combined with the advantages of using the OSTE materials, such as low temperature bonding and possibility for stablesurface modifications, a candidate construction material for future development of systems for DNA analysis is at hand.OSTE can serve as a flexible support for an adsorbed film of a conducting polymer with the possibility for future applicationssuch as electronic interfaces in microsystems. In this project, a film of PEDOT:PSS with the electrical resistance of ~5 kΩ wascreated by adsorption to an flexible OSTE material. Furthermore, results suggest that it is possible to further optimize theconductivity and water resistance of PEDOT:PSS films on OSTE.

Status and distribution of the Mediterranean monk seal in the northeastern Mediterranean were studied between October 2003 and December 2005. In total, 7 research cruises and 8 research visits were carried out to the region in the study period. The study was generally focused on two regions. First region was mainly around the Cilicia basin in the northeastern Mediterranean where a known Mediterranean monk seal colony (the Cilician colony) inhabits. Second region was around the Gulf of iskenderun where the population status of the monk seals was unknown.In the northeastern Mediterranean, all monk seal caves especially those used for breeding have been checked for whelping and monitored during the study period. In total, 7 pups were found including one death pup in the study period. Observations of the breeding behavior of the species indicated that, whelping also takes place in 2 new caves in addition to the 39 caves already reported for the study area in the earlier studies. Increase in the number of breeding caves showed that the breeding sites of the species has been expanded within the last 5 years. The Cilician colony size was estimated as 30 individuals in 2005. Identification catalog for each individual in the Cilician colony was prepared. Finally, population viability analysis (PVA) for the Cilician monk seal colony was carried out by evaluating the vital parameters of the species, which have been collected since 1994. This analysis was carried out for both pre-conservation phase and the post-conservation phase. In post conservation phase, the survival and fecundity rate of the Cilician colony was found as 0.976 and 0.169 respectively whereas these values were estimated as 0.902 and 0.200 respectively in pre-conservation phase. It was found that there is a 26.9% risk that the monk seal colony abundance will fall below the existing level (30 individuals) at least once during the next 20 years and there is also 0.2% risk that the monk seal colony abundance will fall below 12 at least once during the next 20 years. The risk was found as 21.7% by evaluating the status of the colony in preconservation phase. It was the first PVA study for this species, in which all the parameters used in the analysis were based on the study population, instead of the congeneric Hawaiian Monk Seal. Prior to this study, although monk seals have been frequently sighted by local people in the region, status of the Mediterranean monk seals and presence of the suitable habitats for the species in the Gulf of iskenderun was unknown. Therefore, population status of the Mediterranean monk seal in the Gulf of iskenderun and suitable habitats were investigated. In total, 30 caves were discovered and 7 of them were classified suitable for the Mediterranean monk seal. In addition, a monk seal information network was established in the region in order to gain information about the species especially when the individuals are sighted (alive, injured or death). In total, 51 sighting reports were obtained from local people via the Mediterranean monk seal information network during the study period. Since there are sampling difficulties due to critical status of the Mediterranean monk seal, alternative sampling techniques were investigated in order to find answers to questions related to the monk seal colony inhabiting in the northeastern Mediterranean. For identification of the individuals, comparison of the individuals and monitoring the individuals, 3D model construction technique from photographs was tested as an alternative photoidentification technique for the Mediterranean monk seal. It was found that at least 100 reference points were needed to construct the 3D model of the monk seal.

Rapid development and urbanization of the Lower Florida Keys in the last 30 years has fragmented the habitat of the Lower Keys marsh rabbit (Sylvilagus palustris hefneri) and threatened it with extinction. Current threats exist at multiple spatiotemporal scales and include threats due to development, invasive species, and global climate change. On Boca Chica Key, the Lower Keys marsh rabbit (LKMR) exists as a metapopulation on Naval Air Station-Key West (NASKW). I conducted a population viability analysis to determine the metapopulation's risk of extinction under multiple management scenarios by developing a spatially-explicit, stage-structured, stochastic matrix model using the programs RAMAS Metapop and ArcGIS. These management scenarios include clearance of airfield vegetation, habitat conversion, and control of feral cats as an invasive species. Model results provided the Navy with relative risk estimates under these different scenarios. Airfield clearance with habitat conversion increased extinction risk, but when coupled with feral cat control, risk was decreased. Because of the potential of sea-level rise due to human-induced global climate change, and its projected impact on the biodiversity of the Florida Keys, I estimated the impacts of rising sea levels on LKMR across its geographic distribution under scenarios of no, low (0.3m), medium (0.6m), and high (0.9m) sea-level rise. I also investigated impacts due to 2 treatments (allowing vegetation to migrate upslope and not allowing migration), and 2 land-use planning decisions (protection and abandonment of humandominated areas). Not surprisingly, under both treatments and both land-use planning decisions, I found a general trend of decreasing total potential LKMR habitat with increasing sea-level rise. Not allowing migration and protecting human-dominated areas both tended to decrease potential LKMR habitat as compared with allowing migration and abandoning human-dominated areas. In conclusion, conservation strategies at multiple scales need to be implemented in order to reduce threats to LKMR, such as development, invasive species, and global climate change.

Environmental concerns have led to a rapid increase in renewable energy development and production as the global demand for electricity continues to increase. The intermittent and uncertain nature of electricity generation from renewable sources, such as wind and solar, however, create significant challenges in maintaining power system reliability at reasonable costs. Energy storage and smart-grid technologies are perceived to provide potential solutions to these challenges in modern power systems of different sizes. This work investigates the opportunity to incorporate energy storage in microgrids with renewable energy production, as well as applying smart microgrid management techniques to reduce the lifetime costs while maintaining an acceptable level of reliability. A microgrid consisting of a 5 home community with generation supplied by two propane generators to meet the “N-1” reliability criterion is used as the base case scenario. Actual load data of typical homes is obtained from the industry partner. An equivalent loss of load expectation criterion is used to benchmark the acceptable reliability level. A model is developed to calculate the lifetime operational cost of the base case scenario which is used to assess the benefit of the addition of renewable energy sources, energy storage, and smart microgrid management techniques. A MATLAB program is developed to assess the 20 year operational costs of various combinations of renewable energy sources and battery energy storage, which will be considered the lifetime of the system. The combination of generation and storage which yields the lowest lifetime operational cost is defined as the optimized microgrid, and is used as a basis to determine if additional savings are realized by the implementation of a microgrid operated by a Smart Microgrid Management System (SMMS). The conceptual layout of the proposed SMMS is presented along with identified methods of utilizing in-home thermal storage. The SMMS mechanism is discussed along with proposed functionality, potential methods of employment, and associated development and implementation costs. The microgrid operated by the SMMS is assessed, and its lifetime operational cost is presented and contrasted against the base case microgrid and the optimized microgrid. A power system reliability evaluation of the proposed microgrids are conducted using a probabilistic method to ensure that reliability is not sacrificed by the implementation of a cost-minimized microgrid. A sequential Monte Carlo simulation model is developed to assess the power system reliability of the various microgrid configuration cases. The functionality of this model is verified using an existing reliability assessment program. The results from the presented studies show that the implementation of renewable energy sources, energy storage, and smart microgrid management techniques are an effective way of reducing the operational cost of a remote microgrid while increasing its power system reliability.

Magistrantūros studijų baigaimajame darbe pateikiami tyrimų duomenys apie biologinių preparatų įtaką žieminių kviečių sėklų dygimo energijai bei daigumui, sėklų užterštumui mikromicetais ir augalų ligotumui vegetacijos metu. Tyrimo objektas – žieminiai kviečiai `Širvinta 1` Tyrimo metodai: Žieminių kviečių sėklų mikromicetai išskirti ir išauginti laboratorijoje. Žieminių kviečių lapų ir pašaknio ligų apskaitos atliktos trijų augimo tarpsnių metu (Dabkevičius, Brazauskienė, 2007). Biologinių preparatų įtakos, ekologiškiems žieminiams kviečiams tyrimai atlikti Aleksandro Stulginskio universiteto Agronomijos fakulteto Biologijos ir augalų biotechnologijos instituto laboratorijoje ir agroekologijos centro ekologinės gamybos ūkyje 2012-2013 metais. Sėklų dygimo energija ir daigumas buvo nustatinėjamas praėjus po jų apvėlimo 24, 480 ir 960 valandoms. Užkrėstumas mikromicetais - po 24 ir 960 val. Nustatyta, kad biologiniais preparatais apdorotų žieminių kviečių sėklų dygimo energija ir daigumas, praėjus po apvėlimo 480 val., padidėjo esmingai 4,45 ir 0,62 proc.vnt., lyginant su tirtomis po 24 val.. Po apvėlimo praėjus 960 val. lyginant su apveltomis sėklomis po 480 val. dygimo energija sumažėjo nežymiai, bet sėklų daigumas sumažėjo esmingai 0,84 proc.vnt. Dygimo energija lyginant su sėklomis, tirtomis po 24 val. nuo apvėlimo, buvo didesnė 3,45 proc.vnt., bet neesmingai. Biokal 1 praėjus 24 val. po sėklų apdorojimo efektyviai sumažino paviršinį žieminių kviečių užterštumą Penicillium... [toliau žr. visą tekstą] / In this Master’s thesis the investigation data collected during the research which was aimed to find out the influence of biological preparation on germination energy and viability, fungi contamination and diseases of organic winter wheat grain during its vegetation period are presented. The Investigation Object: winter wheat grain “Širvinta 1“ The Methods of the Investigation: the fungi of winter wheat grain were extricated and grown in the laboratory. The results of the winter wheat leaves and roots diseases were recorded during three vegetation phases (Dabkevičius, Brazauskienė, 2007). In 2012-2013 in the centre of Agro-ecology and Biotechnology at Aleksandras Stulginskis University of Agriculture the research was carried out to investigate the effectiveness of biological preparation on organic winter wheat. The germination energy and viability of the grain were recorded after 24, 480 and 960 hours after having treated them with biological preparation. The level of fungi contamination was measured after the periods of 24 and 960 hours. It was stated that after the treatment with biological preparation the germination energy and viability of the grain after the period of 480 hours increased substantially 4,45 and 0,62 % per unit in comparison with the period of 24 hours. Ater 960 hours the germination energy decreased slightly comparing to that after 480 hours whereas its viability decreased substantially 0,84 % per unit. The germination energy level, in comparison with... [to full text]

This study focuses on the design, construction and operation of a distributed generation system based on micro-hydro technology. The project is sited in the Totara Valley, a small rural community approximately 70km from the Massey University, Turitea campus, Palmerston North. The Massey University Centre for Energy Research (MUCER) has a long history of renewable energy research within the Totara Valley community. This project complements these existing schemes and provides a foundation for future research into distributed generation technologies. The project encompasses the following objectives: - to gain practical experience in the design, engineering and implementation of a distributed generation system in rural New Zealand; - to evaluate contemporary micro-hydro technology and compare the performance of this equipment in a theoretical and practical context; - to identify barriers that hinder the widespread adoption of micro-hydro systems in rural New Zealand; - to develop a spreadsheet based life cycle costing tool. The results from this study demonstrate that economic considerations are the fundamental aspect to be considered when assessing the long-term viability of these projects. The viability of micro-hydro projects are primarily determined by four factors: - the volume and head (height) of water available above the turbine site; - the length and therefore the cost of the pipeline required for transporting water to the turbine; - the legal and administrative costs involved in obtaining a resource consent to maintain access to the water resources; - the prices received and paid for electricity. Considerable charges were payable to the local authority to secure and maintain the right to harness the water resources at this site. This cost contributed considerable risk to the project and creates a significant barrier to establishing similar systems at other sites. The reduction of resource consent charges to levels that fairly reflect the negligible environmental impacts of these projects would encourage the adoption of this technology and deliver benefits to rural New Zealand communities.