Spelling suggestions: "subject:"villous atrophy"" "subject:"guillous atrophy""
1 |
Baja frecuencia de positividad serológica en pacientes con biopsias histológicamente compatibles con enfermedad celiaca en Perú.Arévalo, F., Roe, E., Arias-Stella Castillo, J., Cárdenas, J., Montes, P., Monge, E. 24 March 2014 (has links)
Objetivo: estudiar la frecuencia de positividad de las pruebas
serológicas en pacientes con biopsias compatible con enfermedad
celiaca.
Material y métodos: estudio transversal. Se incluyeron pacientes
con biopsia duodenal histológicamente compatible con enfermedad
celiaca y determinación de anticuerpos antigliadina, antiendomisio
y antitransglutaminasa IgA. Definimos como caso de
enfermedad celiaca a quienes tuvieran biopsia positiva y anticuerpos
antiendomisio y/o antitransglutaminasa positivos.
Resultados: 31 pacientes fueron incluidos de los cuales 6 fueron
antiendomisio positivo, 5 fueron antitransglutaminasa positivo
y antigliadina fue positivo en 14. Por lo tanto de 31 pacientes con
cambios histológicos compatibles con enfermedad celiaca sólo 10
tuvieron serología diagnóstica. Sólo uno de los pacientes tuvo positividad
tanto para antitransglutaminasa como para antiendomisio.
Conclusiones: a) encontramos que la mayoría de biopsias de
duodeno con un cuadro histológico sugerente de enfermedad celiaca
no se corresponden con serología diagnóstica de esta enfermedad;
b) encontramos baja coincidencia en la positividad serológica
entre antiendomisio y antitransglutaminasa. / Objective: to study the frequency of positive serology for celiac
disease (CD) in patients with duodenal biopsies suggestive of
this disease. Material and methods: cross sectional study. We included
patients with duodenal biopsies histologically compatible with CD
and antigliadin, antiendomysial and IgA antitransglutaminase antibodies.
We defined a “case” of CD if there was a positive biopsy
and either antiendomisial or antitransglutaminase positive antibodies.
Results: thirty one patients were included in our study. Six
were antiendomysial positive and 5 antitransglutaminase positive
while the antigliadin was positive in 14 cases. Therefore, out of
31 patients only 10 had a serology compatible with CD and only
one had positive both antibodies, antiendomysial and antitransglutaminase.
Conclusions: a) we have found that most of the duodenal
biopsies compatible with CD are not diagnosed with positive serology;
and b) we found a low correlation between serological diagnostic
tests.
|
2 |
HIV enteropathy: crypt stem and transit cell hyperproliferation induces villous atrophy in HIV/Microsporidia-infected jejunal mucosaBatman, Philip A., Kotler, D.P., Kapembwa, M.S., Booth, D., Orenstein, J.M., Scally, Andy J., Griffin, G., Potten, C.S. January 2007 (has links)
Objectives: The study aim was to analyse the kinetics of stem and transit cells in the crypts of jejunal mucosa infected with HIV and Microsporidia.
Design: The size of villi, depth of crypts and proliferative activity of transit and stem cells in jejunal mucosa were measured using morphometric techniques.
Methods: The surface area/volume ratio (S/V) of jejunal biopsies was estimated under light microscopy using a Weibel graticule. Crypt length was measured by counting enterocytes along the crypt side from the base to the villus junction, and the mean crypt length was calculated. The S/V and crypt lengths of the jejunal mucosa of 21 HIV and Microsporidia-infected test cases were compared with 14 control cases. The labelling index in relation to the crypt cell position of 10 of the test cases was analysed compared with 13 control cases.
Results: Differences were found in the S/V and crypt length, and there was a negative correlation between S/V and crypt length in test and control cases combined. Cell labelling indices fell into low and high proliferation groups. There were significant differences in labelling indices between low proliferation test cases and controls, between high proliferation test cases and controls, and between high and low proliferation test cases.
Conclusion: Villous atrophy induced by HIV and Microsporidia is attributed to crypt cell hyperplasia and the encroachment of crypt cells onto villi. These infections induce crypt hypertrophy by stimulating cell mitosis predominantly in transit cells but also in stem cells. Increased stem cell proliferation occurs only in high proliferation cases.
|
3 |
Deciphering the Transcriptomic Heterogeneity of Duodenal Coeliac Disease BiopsiesWolf, Johannes, Willscher, Edith, Loeffler-Wirth, Henry, Schmidt, Maria, Flemming, Gunter, Zurek, Marlen, Uhlig, Holm H., Händel, Norman, Binder, Hans 26 January 2024 (has links)
Coeliac disease (CD) is a clinically heterogeneous autoimmune disease with variable presentation
and progression triggered by gluten intake. Molecular or genetic factors contribute to disease
heterogeneity, but the reasons for different outcomes are poorly understood. Transcriptome studies
of tissue biopsies from CD patients are scarce. Here, we present a high-resolution analysis of the
transcriptomes extracted from duodenal biopsies of 24 children and adolescents with active CD and
21 individuals without CD but with intestinal afflictions as controls. The transcriptomes of CD patients
divide into three groups—a mixed group presenting the control cases, and CD-low and CD-high
groups referring to lower and higher levels of CD severity. Persistence of symptoms was weakly
associated with subgroup, but the highest marsh stages were present in subgroup CD-high, together
with the highest cell cycle rates as an indicator of virtually complete villous atrophy. Considerable
variation in inflammation-level between subgroups was further deciphered into immune cell types
using cell type de-convolution. Self-organizing maps portrayal was applied to provide high-resolution
landscapes of the CD-transcriptome. We find asymmetric patterns of miRNA and long non-coding
RNA and discuss the effect of epigenetic regulation. Expression of genes involved in interferon
gamma signaling represent suitable markers to distinguish CD from non-CD cases. Multiple pathways
overlay in CD biopsies in different ways, giving rise to heterogeneous transcriptional patterns,
which potentially provide information about etiology and the course of the disease.
|
Page generated in 0.1113 seconds