Coreplication experiments have revealed that replication of turnip yellow mosaic virus (TYMV) RNA in turnip protoplasts is cis-preferential. Genomes encoding mutant p141 or p66, proteins essential for virus replication, were inefficiently rescued by a helper genome. One model for the cis-preferential replication of TYMV is that p66 and p141 form a complex that associates with the RNA from which they are translated, limiting their availability in trans. Three types of experiments were used in this study in an attempt to obtain physical evidence for the hypothetical interaction between p66 and p141. Immunoprecipitations from in vitro translation reactions using antiserum that recognizes p66 (and its progenitor, p206) coprecipitate p141, indicating that the proteins form a complex in vitro. The results of coimmunoprecipitations of translation products with in-frame deletions did not lead to definitive information about interaction domains. p66 and the helicase domain of p141 do not detectably interact in the yeast two-hybrid system or in GST fusion interaction assays. Problems with the expression of full length p141 fusions make conclusions about the interaction of other p141 domains with p66 not possible at this time. Since the helicase domain of p141 does not appear to interact with p66, future experiments will focus on obtaining expression of smaller domains of p141, outside the helicase domain, and determining if they interact with p66. Variations to the model that do not necessitate the direct interaction between p66 and p141 are also considered. / Graduation date: 1997

Includes bibliographical references (leaves [102]-120). / 5, 120, [ca. 50] leaves, [3] leaves of plates : ill. ; 30 cm / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Studies the structure of the cucumber mosaic virus genome and the control of its expression. / Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1988

Includes Appendix listing other publications by the author. / Includes bibliographical references (leaves 134-181). / vi, 182 leaves : ill., photos ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Alfalfa mosaic virus was isolated from lucerne (Medicago sativa) plants with a variety of disease symptoms. Experiments showed that each isolate was biologically distinct and that the host range and symptomatology of each isolate was affected by the environmental condition. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Pathology, 1990

Includes bibliographical references (leaves 108-123). / xi, 123 leaves : ill., photos ; 26 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The comparative studies carried out suggest that tomato aspermy virus and the Q strain of cucumber mosaic virus are sufficiently similar to be included in the same taxonomic group. Nevertheless, the two viruses are distinct and the present nomenclature should be retained. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Pathology, 1975

Typescript (photocopy) / Includes bibliographical references (leaves 84-94). / xi, 94 leaves, [35] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Eight cucumovirus isolates were examined with respect to their host range, antigenic properties and nucleic acid composition. / Thesis (Ph.D.)--Dept. of Plant Pathology, University of Adelaide, 1983

Typescript (photocopy) / Includes bibliographical references (leaves 84-94). / xi, 94 leaves, [35] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Eight cucumovirus isolates were examined with respect to their host range, antigenic properties and nucleic acid composition. / Thesis (Ph.D.)--Dept. of Plant Pathology, University of Adelaide, 1983

Bibliography : leaves 92-99. / v, 99, [37] leaves, [14] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1992

Includes bibliographical references (leaves 147-171). Studies factors affecting the rate of epidemic progress of cucumber mosaic virus in Lupinus angustifolius.

Typescript; author's name is corrected in pen on t.p. Includes bibliographical references (leaves 52-55). A study has been made of an undescribed virus disease, Centrosema mosaic. Field observations on its occurence, ecology, symptoms, and host range, together with experimental data on mechanical, insect and other methods of transmission are recorded.

Includes bibliographical references (leaves 147-171). / xx, 171 leaves : ill. (some col.), photos ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Studies factors affecting the rate of epidemic progress of cucumber mosaic virus in Lupinus angustifolius. / Thesis (Ph.D.)--Dept. of Crop Protection, University of Adelaide,1992