The purpose of this research was to evaluate and compare the performance, aesthetic and material properties of women’s t-shirts composed of three different sustainable fibers (lyocell, organic cotton, and viscose from bamboo) before and after repeated home laundering. A convenience sample was comprised of thirty-nine t-shirts. Thirteen black t-shirts from each fiber content were used for testing according to ASTM and AATCC standards. The evaluations and measurements were performed initially and after wash intervals one, five, ten, and twenty. The t-shirts were tested for fabric weight, fabric count, color fastness, smoothness appearance, pilling, dimensional stability and skewness.