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Transporte de água em células de melanona murino S91 submetidas a condições anisosmóticas / Water transport in murine melanoma S91 cells submitted to anisosmotic conditionsSilva, James Fernando Malta da 06 June 2007 (has links)
Uma das principais necessidades da célula é a regulação do seu ambiente interno. Aparte da considerável importância teórica, o transporte de água é de importância prática numa ampla gama de processos, desde a proteção de células na preservação criogênica até os efeitos de certos hormônios em alguns tecidos. Virtualmente todas as células são submetidas a transições osmóticas durante o seu período de vida, uma vez que tanto o metabolismo intracelular quanto o transporte por membranas produzem flutuações nas concentrações dos solutos osmoticamente ativos. A regulação de volume celular é um fenômeno ubíquo e permite, às células, manter o seu volume normal. Células submetidas a choques anisosmóticos agudos sofrem rápidas alterações de volume (dependentes do gradiente osmótico e da permeabilidade da membrana à água e osmólitos) podendo ou não ser seguidas de lentas alterações regulatórias de volume. Assim, o objetivo do presente trabalho visou esclarecer alguns aspectos do transporte de água em células de melanoma murino S91 submetidas a condições anisosmóticas. Células de melanoma murino S91, foram mantidas em meio de cultura F12 HAM (290 mOsm.kgH2O-1). As medidas morfométricas das mudanças relativas de volume foram realizadas usando-se um sistema de aquisição e análise de imagens (Image Pro-Lite, Media Cybernetics). As células foram expostas tanto a choques hiposmóticos agudos (190 mOsm.kgH2O-1) como a choques hiperosmóticos agudos (350 mOsm.kgH2O-1) em diferentes temperaturas (de 17 a 37 oC) e em diferentes doses (de 0,001 a 1000 µM) de HgCl2, um bloqueador de aquaporinas (AQP). Os resultados sugerem que: (i) o tempo de regulação de volume em células de melanoma murino S91 é dependente da temperatura; (ii) o fluxo osmótico de água apresenta valores de Energia de Ativação compatíveis com aqueles propostos para o trânsito de água através de aquaporinas (Ea < 6 kcal.mol-1); (iii) o HgCl2 afeta de forma dose dependente as respostas osmóticas em células de melanoma murino S91 e sugerem a presença de mais de um tipo de AQP. Nestas condições as concentrações necessárias para reduzir ao máximo a permeabilidade osmótica à água estão localizadas na faixa de 0,1-1,0 µM HgCl2. / One of the major needs of living cells is the regulation of their internal environment. Apart from being of considerable theoretical importance, the transport of water is of practical importance in a broad range of process, from the protection of cells undergoing cryogenic preservation to the effects of certain hormones in some tissues. Virtually all the cells are submitted the osmotic transitions during their period of life, because both intracellular metabolism and transmembrane transport produce fluctuations in concentrations of osmolytes. The regulation of cellular volume is a phenomenon ubiquitous and allows, to the cells, to keep their normal volume. Cells subjected to acute anisosmotic shocks suffer from fast alterations in volume (depending on the osmotic gradient and on the permeability of the membrane to the water and osmotically active substances), and followed or not by a slow volume regulation response. Thus, the present work aims to clarify some aspects of the water transport in murine melanoma S91 cells subjected to anisosmotic conditions. S91 murine melanoma cells were grown in F12 HAM medium (290 mOsm.kgH2O-1). Morphometric measurements of relative changes in cell volume were performed using a video microscopy system and a PC software (Image Pro-Lite, Media Cybernetics). The experimental cells were exposed either to acute hyposmotic shocks (190 mOsm.kgH2O-1) or to acute hyperosmotic shocks (350 mOsm.kgH2O-1), in different temperatures (ranging from 17 to 37 oC) and in the presence of HgCl2 (from 0,001 to 1000 µM), an aquaporin blocker. The results of the present study indicate that: (i) the time of volume regulation in S91 murine melanoma cells is dependent on temperature; (ii) the values of osmotic water flow are compatible with activation energy through aquaporins (E < 6 kcal.mol-1) and (iii) HgCl2 treatments affect osmotic behavior of S91 murine melanoma cells in a dose-response manner and also suggest the presence of more than one type of aquaporin. Minimum osmotic water permeabilities were observed in a range of µM HgCl2 treatments.
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Transporte de água em células de melanona murino S91 submetidas a condições anisosmóticas / Water transport in murine melanoma S91 cells submitted to anisosmotic conditionsJames Fernando Malta da Silva 06 June 2007 (has links)
Uma das principais necessidades da célula é a regulação do seu ambiente interno. Aparte da considerável importância teórica, o transporte de água é de importância prática numa ampla gama de processos, desde a proteção de células na preservação criogênica até os efeitos de certos hormônios em alguns tecidos. Virtualmente todas as células são submetidas a transições osmóticas durante o seu período de vida, uma vez que tanto o metabolismo intracelular quanto o transporte por membranas produzem flutuações nas concentrações dos solutos osmoticamente ativos. A regulação de volume celular é um fenômeno ubíquo e permite, às células, manter o seu volume normal. Células submetidas a choques anisosmóticos agudos sofrem rápidas alterações de volume (dependentes do gradiente osmótico e da permeabilidade da membrana à água e osmólitos) podendo ou não ser seguidas de lentas alterações regulatórias de volume. Assim, o objetivo do presente trabalho visou esclarecer alguns aspectos do transporte de água em células de melanoma murino S91 submetidas a condições anisosmóticas. Células de melanoma murino S91, foram mantidas em meio de cultura F12 HAM (290 mOsm.kgH2O-1). As medidas morfométricas das mudanças relativas de volume foram realizadas usando-se um sistema de aquisição e análise de imagens (Image Pro-Lite, Media Cybernetics). As células foram expostas tanto a choques hiposmóticos agudos (190 mOsm.kgH2O-1) como a choques hiperosmóticos agudos (350 mOsm.kgH2O-1) em diferentes temperaturas (de 17 a 37 oC) e em diferentes doses (de 0,001 a 1000 µM) de HgCl2, um bloqueador de aquaporinas (AQP). Os resultados sugerem que: (i) o tempo de regulação de volume em células de melanoma murino S91 é dependente da temperatura; (ii) o fluxo osmótico de água apresenta valores de Energia de Ativação compatíveis com aqueles propostos para o trânsito de água através de aquaporinas (Ea < 6 kcal.mol-1); (iii) o HgCl2 afeta de forma dose dependente as respostas osmóticas em células de melanoma murino S91 e sugerem a presença de mais de um tipo de AQP. Nestas condições as concentrações necessárias para reduzir ao máximo a permeabilidade osmótica à água estão localizadas na faixa de 0,1-1,0 µM HgCl2. / One of the major needs of living cells is the regulation of their internal environment. Apart from being of considerable theoretical importance, the transport of water is of practical importance in a broad range of process, from the protection of cells undergoing cryogenic preservation to the effects of certain hormones in some tissues. Virtually all the cells are submitted the osmotic transitions during their period of life, because both intracellular metabolism and transmembrane transport produce fluctuations in concentrations of osmolytes. The regulation of cellular volume is a phenomenon ubiquitous and allows, to the cells, to keep their normal volume. Cells subjected to acute anisosmotic shocks suffer from fast alterations in volume (depending on the osmotic gradient and on the permeability of the membrane to the water and osmotically active substances), and followed or not by a slow volume regulation response. Thus, the present work aims to clarify some aspects of the water transport in murine melanoma S91 cells subjected to anisosmotic conditions. S91 murine melanoma cells were grown in F12 HAM medium (290 mOsm.kgH2O-1). Morphometric measurements of relative changes in cell volume were performed using a video microscopy system and a PC software (Image Pro-Lite, Media Cybernetics). The experimental cells were exposed either to acute hyposmotic shocks (190 mOsm.kgH2O-1) or to acute hyperosmotic shocks (350 mOsm.kgH2O-1), in different temperatures (ranging from 17 to 37 oC) and in the presence of HgCl2 (from 0,001 to 1000 µM), an aquaporin blocker. The results of the present study indicate that: (i) the time of volume regulation in S91 murine melanoma cells is dependent on temperature; (ii) the values of osmotic water flow are compatible with activation energy through aquaporins (E < 6 kcal.mol-1) and (iii) HgCl2 treatments affect osmotic behavior of S91 murine melanoma cells in a dose-response manner and also suggest the presence of more than one type of aquaporin. Minimum osmotic water permeabilities were observed in a range of µM HgCl2 treatments.
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Natriuretic Peptides As A Humoral Link Between The Heart And The Gastrointetsinal SystemAddisu, Anteneh 18 March 2008 (has links)
Natriuretic peptides are a family of hormones released by several different tissues and exert various physiological functions by coupling with cell surface receptors and increasing intracellular cyclic gyanylyl monophosphate (cGMP). Atrial Natriuretic Peptide (ANP) and B-type Natriuretic Peptide (BNP) are released in response to mechanical stretch of the atrial or ventricular myocardium, respectively and their plasma level is markedly elevated during myocardial infarction and heart failure. Heart failure in turn is associated with symptoms suggestive of perturbed gastrointestinal function such as nausea, indigestion and malabsorption.
Intragastric pressure was monitored using a balloon catheter in anesthetized mice. The pressure before and after treatment with a 10 ng/g intravenous dose of ANP, BNP, CNP or vehicle was compared and analyzed. All the natriuretic peptides significantly decreased intragastric pressure compared to vehicle. These effects were attenuated or absent in natriuretic peptide receptor type-A (NPR-A) knockout mice. Furthermore, the effect of BNP on gastric emptying and intestinal absorption was examined using a meal consisting of fluorescence labeled dextran gavage fed to awake mice. BNP significantly decreased gastric emptying and absorption as compared to vehicle control. Using a cryoinfarction acute myocardial injury model, our investigation showed that mice with acute cryoinfarction had a significantly lower gastric emptying and absorption of a gavage fed meal compared to sham. Circulating BNP levels were significantly higher in the infarcted mice compared to controls. Immunostaining showed amplified distribution of the non-muscle myosin type-II (MCH-II) in BNP treated mice. MCH-II is involved in movement of intestinal villi.
In summary, natriuretic peptides in general and BNP in particular, have gastrointestinal effects including reduced gastric contractility, emptying and absorption. In addition to their effect on smooth muscle relaxation mediated by cGMP, natriuretic peptides appear to have an effect on distribution of MHC-II in cells of the intestinal villi.
We postulate that these effects are aimed at mediating a 'communication' between the cardiovascular and gastrointestinal systems. Further characterization of such a link will not only add a dimension to the understanding of the pathophysiology of heart failure but also enhances the search for further therapeutic targets.
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Mechanisms of Müller and bipolar cell swelling in the healthy and pathologically altered retina / Mechanismen der Müller- und Bipolarzellschwellung in der normalen und pathologisch veränderten NetzhautVogler, Stefanie 07 January 2016 (has links) (PDF)
The topic of the thesis is the mechanisms of cellular volume regulation in the rat retina. Müller cells as main macroglial cells of the retina are supposed to play important roles in the regulation of the retinal ion- and osmohomeostasis and, thus, in the regulation of the extracellular space volume. In the first part of the thesis, signaling pathways were determined which are involved in the regulation of the volume of Müller glial cells and bipolar cells, the main second-order cells of the retina, in the healthy rat retina. The topic of the second part of the thesis is the evaluation of gliotic alterations of Müller cells in a transgenic rat model of retinal degeneration (CMV-PKD21/703 HA rats), in order to obtain indications for a pathogenic role of reactive glial cells in the development of retinal degeneration and edema. Various methods were used including immunohistochemical stainings, real-time RT-PCR, patch-clamp recordings, and cell swelling experiments. The data suggest that both neurons and reactive Müller cells may contribute to formation of retinal edema. In contrast to Müller cells, bipolar cells are apparently not capable to regulate the extracellular space volume in the healthy retina. However, reactive Müller cells are impaired in their capability to regulate retinal water and ion homeostasis. Impaired regulation of the extracellular space volume may result in neuronal hyperexcitation and degeneration.
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Redistribution of Hepatocyte Chloride During L-Alanine UptakeWang, Kening, Wondergem, Robert 01 September 1993 (has links)
We used ion-sensitive, double-barrel microelectrodes to measure changes in hepatocyte transmembrane potential (Vm), intracellular K+, Cl-, and Na+ activities (aik, aCliand aNai), and water volume during l-alanine uptake. Mouse liver slices were superfused with control and experimental Krebs physiological salt solutions. The experimental solution contained 20 μml-alanine, and the control solution was adjusted to the same osmolality (305 mOsm) with added sucrose. Hepatocytes also were loaded with 50 m m tetramethylammonium ion (TMA+) for 10 min. Changes in cell water volume during l-alanine uptake were determined by changes in intracellular, steady-state TMA+ activity measured with the K+ electrode. Hepatocyte control Vm was -33±1 mV. l-alanine uptake first depolarized Vm by 2±0.2 mV and then hyperpolarized Vm by 5 mV to-38±1 mV (n = 16) over 6 to 13 min. During this hyperpolarization, aNaiincreased by 30% from 19±2 to 25±3 m m (P < 0.01), and aKidid not change significantly from 83±3 m m. However, with added ouabain (1 m m) l-alanine caused only a 2-mV increase in Vm, but now aKidecreased from 61±3 to 54±5 m m (P < 0.05). Hyperpolarization of Vm by l-alanine uptake also resulted in a 38% decrease of aClifrom 20±2 to 12±3 m m (P < 0.001). Changes in Vm and VCl - Vm voltage traces were parallel during the time of l-alanine hyperpolarization, which is consistent with passive distribution of intracellular Cl- with the Vm in hepatocytes. Added Ba2+ abolished the l-alanineinduced hyperpolarization, and aCliremained unchanged. Hepatocyte water volume during l-alanine uptake increased by 12±3%. This swelling did not account for any changes in ion activities following l-alanine uptake. We conclude that hepatocyte aKiis regulated by increased Na+-K+ pump activity during l-alanine uptake in spite of cell swelling and increased Vm due to increased K+ conductance. The hyperpolarization of Vm during l-alanine uptake provides electromotive force to decrease aCli. The latter may contribute to hepatocyte volume regulation during organic solute transport.
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Characterization of Stimulation-induced Volume Changes in the Ca1Region of Rat Hippocampus SlicesGutwein, Amanda Brooke 29 May 2013 (has links)
No description available.
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Differential Regulation of the Hippocampal Taurine Transporter Protein in Rat Brain: Mechanisms Contributing to Neuronal Volume RegulationFreeman, Amanda Noelle 01 August 2013 (has links)
No description available.
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Ion transport mechanisms during hyposmotic regulatory and isosmotic apoptotic volume decreases in a human lens epithelial cells lineChimote, Ameet Ajit 30 September 2009 (has links)
No description available.
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Mechanisms of Müller and bipolar cell swelling in the healthy and pathologically altered retinaVogler, Stefanie 18 September 2015 (has links)
The topic of the thesis is the mechanisms of cellular volume regulation in the rat retina. Müller cells as main macroglial cells of the retina are supposed to play important roles in the regulation of the retinal ion- and osmohomeostasis and, thus, in the regulation of the extracellular space volume. In the first part of the thesis, signaling pathways were determined which are involved in the regulation of the volume of Müller glial cells and bipolar cells, the main second-order cells of the retina, in the healthy rat retina. The topic of the second part of the thesis is the evaluation of gliotic alterations of Müller cells in a transgenic rat model of retinal degeneration (CMV-PKD21/703 HA rats), in order to obtain indications for a pathogenic role of reactive glial cells in the development of retinal degeneration and edema. Various methods were used including immunohistochemical stainings, real-time RT-PCR, patch-clamp recordings, and cell swelling experiments. The data suggest that both neurons and reactive Müller cells may contribute to formation of retinal edema. In contrast to Müller cells, bipolar cells are apparently not capable to regulate the extracellular space volume in the healthy retina. However, reactive Müller cells are impaired in their capability to regulate retinal water and ion homeostasis. Impaired regulation of the extracellular space volume may result in neuronal hyperexcitation and degeneration.
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Regulace objemu astrocytů v průběhu stárnutí / Astrocyte volume regulation during agingEliášová, Barbora January 2015 (has links)
Astrocytes, as one of the glial cell types, have many important functions in healthy functioning of the central nervous system (CNS) but also in its pathology. Since they play a key role in maintenance of ionic, neurotransmitter and water homeostasis in CNS, they possess the ability to regulate their volume. Hypo- or hyperosmotic stress can trigger regulatory volume decrease or increase in astrocytes in order to stabilize their volume. During aging, astrocytes undergo many changes together with the rest of the brain. In order to determine whether these alterations involve also regulatory volume mechanisms, we employed three dimensional morphometry, which comprises confocal microscope scanning of fluorescently labelled astrocytes in brain slices of EGFP/GFAP mice and quantification of astrocyte volume during different pathological stimuli. Time-dependent volume changes of hippocampal astrocytes were recorded while applying either hypoosmotic solution or solution with high extracellular potassium concentration. In the four different age groups studied in the experiment, several differences in volume changes were discovered together with some sex-dependent alterations in astrocyte volume. Additionally, in accordance with previous studies, two subpopulation of astrocytes were identified using...
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