Ce travail expérimental s’intéresse à l’écoulement en aval d’un swirler statique court. L’induction d’un mouvement de swirl est une solution connue pour augmenter le gradient pariétal de vitesse. L’augmentation du frottement pariétal présente un intérêt industriel dans les échangeurs de chaleur pour accroître leurs potentiels de transfert de chaleur et diminuer leurs vitesses d’encrassement. Nous proposons d’approfondir la connaissance des écoulements avec swirl décroissant à faibles nombres de Reynolds pour lesquels l’intérêt énergétique est a priori plus important. Dans un premier temps, le champ d’écoulement est caractérisé à l’aide de méthodes optiques, pour identifier le type de tourbillon caractérisant le swirl. Dans un second temps, la méthode électrochimique est utilisée pour mesurer le frottement pariétal instationnaire. Par analogie, ces mesures permettent d’obtenir une première estimation du transfert de chaleur en écoulement anisotherme. / This experimental work is interested in understanding the flow downstream a short static swirler. Inducing a swirl motion is a known solution to increase the wall velocity gradient. The increase in wall shear stress is useful in industrial heat exchangers in order to enhance their heat transfer capabilities and reduce their fouling rates. We try to go deeper into knowledge of decaying swirl flows, for low Reynolds number for which energy interest is greater a priori. First, we study the flow field with optical methods in order to identify type of vortex characteristics of the swirl. Second, electrochemical method is used to measure the unsteady wall shear stress. By analogy, these measurements give a first estimate of the heat transfer for the case of non-isothermal flows.

Neste trabalho é apresentada a análise do transitório hidráulico com a tensão de cisalhamento na parede do conduto forçado, tendo sido modelada por gradiente harmônico de pressão. É apresentada, também, a comparação dos resultados obtidos com a aplicação do método numérico de MacCormack com a utilização de fator de atrito em relação aos obtidos através da utilização de MacCormack com a introdução da tensão de cisalhamento modelada por gradiente harmônico de pressão, na análise de transitórios hidráulicos em condutos forçados. / This work presents the analysis of the Hydraulic Transient with wall shear stress on the pressurized flow modeled by harmonic Pressure Gradient. Appears also to compare results obtained with the application of the MacCormack numerical method with the use of friction factor with respect to those obtained through use of MacCormack with the introduction of wall shear stress on the pressurized flow modeled by harmonic Pressure Gradient in the analysis of hydraulic transients.

Neste trabalho é apresentada a análise do transitório hidráulico com a tensão de cisalhamento na parede do conduto forçado, tendo sido modelada por gradiente harmônico de pressão. É apresentada, também, a comparação dos resultados obtidos com a aplicação do método numérico de MacCormack com a utilização de fator de atrito em relação aos obtidos através da utilização de MacCormack com a introdução da tensão de cisalhamento modelada por gradiente harmônico de pressão, na análise de transitórios hidráulicos em condutos forçados. / This work presents the analysis of the Hydraulic Transient with wall shear stress on the pressurized flow modeled by harmonic Pressure Gradient. Appears also to compare results obtained with the application of the MacCormack numerical method with the use of friction factor with respect to those obtained through use of MacCormack with the introduction of wall shear stress on the pressurized flow modeled by harmonic Pressure Gradient in the analysis of hydraulic transients.

The thesis deals with the problem of friction during rapid transient 1-D flows in a pipe caused by water hammers. The evolution of the wall shear stress is interpreted in terms of two steps. The first step is the dramatic change of the wall shear stress during the passage of the pressure wave; the corresponding new value of the shear stress is much greater than the value predicted in steady-state. The second step, which begins after the passage of the pressure wave, is a relaxation process; here the shear stress decreases, tending to the new steady-state value corresponding to the new average velocity. The Extended Irreversible Thermodynamics theory is proposed as a tool to model the wall shear stress during the relaxation process. The Friction Relaxation Model presented in this thesis describes both steps of the evolution of the wall shear stress during water hammers, and therefore it enables to take into account the information about the velocity gradient at the wall, which is otherwise not available in 1D modelling.

Phase-contrast magnetic resonance imaging (PC-MRI) is a powerful tool for measuring blood flow and has a wide range of cardiovascular applications. Simulation of PC-MRI from numerical flow data would be useful for addressing the data quality of PC-MRI measurements and to study and understand different artifacts. It would also make it possible to optimize imaging parameters prior to the PC-MRI measurements and to evaluate different methods for measuring wall shear stress. Based on previous studies a PC-MRI simulation tool was developed. An Eulerian-Lagrangian approach was used to solve the problem. Computational fluid dynamics (CFD) data calculated on a fix structured mesh (Eulerian point of view) were used as input. From the CFD data spin particle trajectories were computed. The magnetization of the spin particle is then evaluated as the particle travels along its trajectory (Lagrangian point of view). The simulated PC-MRI data were evaluated by comparison with PC-MRI measurements on an in vitro phantom. Results indicate that the PC-MRI simulation tool functions well. However, further development is required to include some of the artifacts. Decreasing the computation time will make more accurate and powerful simulations possible. Several suggestions for improvements are presented in this report.

Atherosclerosis is a multifactoral inflammatory disease that occurs in predisposed locations in the vasculature where blood flow is disturbed. In vitro studies have implicated reactive oxygen species as mediators of mechanotransduction leading to inflammatory protein expression and ultimately atherogenesis. While these cell culture-based studies have provided enormous insight into the effects of WSS on endothelial biology, the applicability to the in vivo setting is questionable. We hypothesized that low magnitude oscillatory WSS acts through reactive oxygen species (ROS) to increase expression of inflammatory cell adhesion molecules leading to the development of atherosclerotic lesions. The overall objective for this thesis was to develop an in vivo flow model that produces low magnitude oscillatory WSS which could be used to investigate the in vivo molecular mechanisms of mechanotransduction. We created a novel aortic coarctation model using a shape memory nitinol clip. The clip reproducibly constricts the aorta creating a narrowing of the lumen resulting in a stenosis. This mechanical constraint produces a region of flow separation downstream from the coarctation. We have characterized the coarctation in terms of the efficacy, pressure loss, and fluid dynamics. We then measured the endothelial response of shear sensitive redox and inflammatory markers. Lastly, we utilized genetically modified mice and mice treated with pharmacological inhibitors to investigate the mechanisms involved in the expression of WSS induced inflammatory and redox markers. We found that inducing a coarctation of the aorta using a nitinol clip uniquely created a hemodynamic environment of low magnitude oscillatory WSS without a significant change in blood pressure. Using this model we found that the in vivo endothelial phenotype associated with acutely disturbed flow was characterized by increased production of superoxide and increased expression of select inflammatory proteins. In comparison, the phenotype associated with chronically disturbed flow was characterized by a more modest increase in superoxide and increased levels of multiple inflammatory proteins. We determined that in regions of acutely disturbed flow in vivo, VCAM-1 expression was not modulated by reactive oxygen species. Additionally, p47 phox-dependent NADPH Oxidase activity does not have a functional role in WSS induced superoxide generation in the endothelium. In summary, we have created a novel murine model of low magnitude oscillatory WSS that can be used to investigate the in vivo molecular mechanisms associated with atherogenesis. While previous data obtained in vitro indicated that depletion of an individual ROS was sufficient to inhibit flow-induced inflammatory protein expression, our findings, to the contrary, showed that antioxidant treatment in vivo does not inhibit shear-dependent inflammatory protein expression. Our results suggest that atherogenesis in the in vivo environment is significantly more complicated than the in vitro environment and that parallel pathways and compensatory mechanisms are likely activated in vivo in response to WSS. These results could have significant implications in the efficacy of antioxidant treatment of atherosclerosis and could explain the complexity of results observed in clinical trials.

<p>Phase-contrast magnetic resonance imaging (PC-MRI) is a powerful tool for measuring blood flow and has a wide range of cardiovascular applications. Simulation of PC-MRI from numerical flow data would be useful for addressing the data quality of PC-MRI measurements and to study and understand different artifacts. It would also make it possible to optimize imaging parameters prior to the PC-MRI measurements and to evaluate different methods for measuring wall shear stress.</p><p>Based on previous studies a PC-MRI simulation tool was developed. An Eulerian-Lagrangian approach was used to solve the problem. Computational fluid dynamics (CFD) data calculated on a fix structured mesh (Eulerian point of view) were used as input. From the CFD data spin particle trajectories were computed. The magnetization of the spin particle is then evaluated as the particle travels along its trajectory (Lagrangian point of view).</p><p>The simulated PC-MRI data were evaluated by comparison with PC-MRI measurements on an in vitro phantom. Results indicate that the PC-MRI simulation tool functions well. However, further development is required to include some of the artifacts. Decreasing the computation time will make more accurate and powerful simulations possible. Several suggestions for improvements are presented in this report.</p>

The blood is a complex fluid that contains, in addition to water, cells, macro-molecules and a large number of smaller molecules. The physical properties of the blood are therefore the result of non-linear interactions of its constituents, which are influenced by the local flow field conditions. Hence, the local blood viscosity is a function of the local concentration of the blood constituents and the local flow field itself. This study considers the flow of blood-like fluids in generalised 90-degree bifurcating pipes and patient-specific arterial bifurcations relevant to the large aortic branches in humans. It is shown that the Red Blood Cell (RBC) distribution in the region of bifurcations may lead to large changes in the viscosity, with implications on the concentrations of the various cells in the blood plasma. This in turn implies that the flow in the near wall regions is more difficult to estimate and predict than that under the assumption of a homogeneous fluid. The rheological properties of blood are complex and are difficult to measure, since the results depend on the measuring equipment and the inherent flow conditions. We attempt to model the viscosity of water containing different volume fractions of non-deforming RBC-like particles in tubes. The apparent viscosities of the mixtures obtained from these model experiments have been compared to the predictions of the different rheological models found in the literature. The same rheological models have also been used in the different simulations, where the local RBC concentration and local shear rate are used in the viscosity models. The flow simulations account for the non-linearity due to coupling between the flow and fluid rheology. Furthermore, from a physiological perspective, it is shown that oscillatory wall shear stresses are affected by changes in RBC concentration in the regions of the bifurcation associated with atherogenesis. The intrinsic shear thinning rheological property of the blood, in conjunction with stagnation in separated flows, may be responsible for elevated temporal wall shear stress gradients (TWSSG) influencing endothelial cell behaviour, which has been postulated to play a role in the development of atherosclerosis. The blood-like fluid properties along with variations in the RBC concentration could also lead to variations in the developing flow structures in the larger arteries that could influence the work the heart has to bear. / <p>QC 20131206</p>

Red blood cells (RBCs) form approximately 40 to 45% of the human blood volume, and their behaviour and characteristics are the main determinant of blood properties, such as viscosity. RBCs are deformable species and stack together under low shear rate to form aggregates or rouleaux. Flowing RBCs migrate away from the wall leaving a cell-depleted layer known as the cell-free layer (CFL). This layer contributes to the blood viscosity and exchange between the RBCs and the target cells: a thinner CFL enhances the exchange process by reducing the diffusion distance. The formation of this CFL, however, is not yet completely understood. The goal of this study is to improve the understanding of the formation of the CFL in the micro-flow. This was accomplished by studying the effects of changing both the flow rate and the microchannel geometry on blood flow in microchannels. In this work, 10% hematocrit human blood suspensions were prepared in native plasma and flowed through poly-dimethylsiloxane (PDMS) microchannels of 100 μm x 34 μm cross-section. Investigation of the flowing cells was performed by using micro particle image velocimetry (μPIV) coupled with a high-speed camera. First, the high-speed camera images were processed with customized Matlab programs to detect and measure the CFL thickness and the RBC aggregates sizes. Second, the blood flow velocity profiles were measured using μPIV in order to determine the actual flow rate, the RBCs’ centerline velocity, and the shear rate. The results showed that the increase in both flow rate and shear rate significantly reduced the CFL thickness and RBC aggregates size. Comparison of the upstream and downstream measurements in the bifurcating microchannel showed that the change in microchannel geometry did not significantly influence CFL thickness and RBC aggregate size, while within the daughter branches, RBCs tended to flow close to the inner wall resulting in an undetectable CFL at the inner wall and in a larger CFL at the outer wall of the branch. These in vitro results quantitatively relate CFL thickness and RBC aggregate size at different shear rates. The findings are of immediate interest regarding the understanding of microcirculation and improved designs of microchips.

In this work, we have developed user interactive capabilities that allow us to perform segmentation and manipulation of patient-specific geometries required for Computational Fluid Dynamics (CFD) studies, entirely in image domain and within a single platform of ‘IAFEMesh'. Within this toolkit we have added commonly required manipulation capabilities for performing CFD on segmented objects by utilizing libraries like ITK, VTK and KWWidgets. With the advent of these capabilities we can now manipulate a single patient specific image into a set of possible cases we seek to study; which is difficult to do in commercially available software like VMTK, Slicer, MITK etc. due to their limited manipulation capabilities. Levelset representation of the manipulated geometries can be simulated in our flow solver (SCIMITAR-3D) without creating any surface or volumetric mesh. This image-levelset-flow framework offers few advantages. 1) We don't need to deal with the problems associated with mesh quality, edge connectivity related to mesh models, 2) and manipulations like boolean operation result in smooth, physically realizable entities which is challanging in mesh domain. We have validated our image-levelset-flow setup with the known results from previous studies. We have modified the algorithm by Krissian et al. and implemented it for the segmentation of Type-A aortic dissection. Finally, we implemented these capabilities to study the hemodynamics in Type-A aortic dissection. Our image based framework is a first of its kind and the hemodynamic study of Type-A dissection too is first study onto the best of our knowledge.