The plant peroxidases (EC1.11.17) exit as a large family of isozymes. These isozymes have more than 50% amino acid sequence differences. The function of Wasaba japonica peroxides plays the role as IAA oxidases. The kinetics result shows Wasabia japonica peroxidases displayed affinity (Km = 17.1 £gM) for IAA. The kinetics results in Wasabia japonica peroxidases display affinity (Km = 80.6 £gM) for syringaldazine. LC/MS/MS technique described the data that has proven to be a method for identification and characterization of proteins. The soluble proteins extracted form Wasabia japonica was purified by gel filtration chromatography and two-dimensional gel electrophoresis (2-DE). LC-MS/MS analyses of 2-DE gel spots and identify proteins structure based on the protein fragmentation characteristics. The Mascot Search Results showed that Wasabia japonica peroxidase has a significant similarity (10%) with Arabidopsis thaliana peroxidase.

The diploma thesis deals with content substances with antioxidant effects in green spice. In the literary part, green spices, antioxidants are defined, and lesser known species are introduced, as well as the determination methods used in the practical part. In the practical part are given the individual determinations. The DPPH method was used to determine the antioxidant capacity, to determine the nitrate content ISE method was used, the flavonoids were determined using the Folin-Ciocalteaue reagent, and the standard in the phenols was gallic acid. Vitamin C was determined by liquid chromatography.