With the rising popularity of artisanal cheese in the United States, the soft washed rind category has emerged as a fast-growing segment of the marketplace. There is much anecdotal evidence to suggest a common sensorial defect in soft washed rind cheese is a gritty/sandy texture attributed to crystal growth on the rind of these cheeses A preliminary study was undertaken to develop a set of criteria to visually identify crystals found on soft washed rind cheeses. Single crystal identities were presumptively determined using polarized light microscopy (PLM), and cross-checked using powder x-ray diffractometry (PXRD). Two distinct crystal groupings were determined based on these metrics. Group 1 crystals had high birefringent coloring, angle of extinction (AE) ≈ 90°, and were smaller and less circular than Group 2 crystals (P<0.05). Group 2 crystals had no birefringent coloring and AE ≈ 18°. Using established mineralogical data, Group 1 and Group 2 crystals were identified to be struvite and ikaite, respectively. These crystalline bodies are situated in an amorphous cheese matrix (i.e. smear), which create difficulties when examining via PLM and PXRD, leading to high background noise. To remedy these issues, a novel method for harvesting crystals was developed. Smear scrapings were immersed in NaOH (pH=10) to dissolve smear material, which resulted in improved PLM and PXRD performance. A subsequent observational study was conducted to understand the prevalence of surface crystals and grittiness associated with washed rind cheeses sourced from the U.S.A. and Europe. Crystal types were identified via PXRD and PLM. Crystal size and shape (circularity) metrics were determined via PLM and image analysis. A descriptive sensory panel (n=12) was used to evaluate grittiness presence and intensity. Identified crystal types included ikaite, struvite, calcite, and brushite. Mean crystal length and area ranged from ~30μm to ~1100μm, and ~500μm2 to ~200,000μm2, respectively. Sensory perception threshold for grittiness occurred at a mean crystal length of ~70μm and mean crystal area of ~2900μm2. Below these threshold levels, cheeses presented with negligible grittiness. Above these threshold levels, grittiness was highly correlated with crystal length and area (r=0.93 and 0.96, respectively; P<0.001). These results indicate surface crystals have a direct impact on the sensorial quality of soft washed rind cheeses.

El Perú cuenta con resultados económicos positivos en los últimos años, inclusive mayores al promedio de la región. Una de las consecuencias del crecimiento económico del país es el incremento del consumo. Además, resalta el hecho de que los niveles socioeconómicos medios se encuentran muy dedicados al hogar, realizando más compras de artículos de primera necesidad, de cuidado personal y de limpieza, asegurándose de obtener la mejor calidad. Debido a este ambiente favorable, se decidió realizar un plan de introducción de detergente líquido al mercado peruano. El producto se ofrecerá a un mercado en constante desarrollo y con alto potencial de crecimiento: al NSE C y D. La rivalidad entre los competidores es considerable y el mercado se limita a pocas empresas con mucha participación. Sin embargo, la categoría es tan grande que se espera encontrar un espacio dentro de un mercado que está todavía en crecimiento sobre el cual desarrollar el producto, esto con una oferta volumen/precio satisfactoria y debidamente difundida mediante actividades promocionales. Esta introducción del producto será por medio de la importación, contando con una operación pequeña que sea eficiente y que sus actividades se apoyen con el uso de la tecnología. Este plan de introducción tendrá como orientación preliminar la penetración del producto en el mercado. Esto permite tener cierta rapidez en las decisiones, que serán necesarias si es que se quiere lograr un impacto y captar a los potenciales compradores con la estrategia de introducción. / Peru has had positive results in recent years, even over the regional average. One of the consequences of economic growth is the consumption increasing. Also, it´s important to emphasize that middle class socioeconomic levels are very dedicated to home; so, they are shopping more staple, personal care products as well as for cleaning. Middle class wants to be sure about getting best quality. Due to this favorable environment, it was decided to make a plan for introducing the liquid detergent to the Peruvian market. The product will be offered for a market in constant development and with high potential: to C and D socioeconomic level. Rivalry between the competitors is hard, so, the market is limited to a few companies with a large market share for each one. However, the product category is so large that it´s expected to find inside this a place for developing the product; all of these, taking advantage about a market that is still growing. This will be done with a satisfactory volume / price offer and it'll be disseminated through promotional activities. The product introduction will be done by imports, making a small operation which is efficient and whose activities are supported with use of technology. This introduction plan will have, as a preliminary orientation, the penetration of the product in the market. This allows to have the require speed in the taking of decisions, the ones will be used to make an impact and to get potential buyers with strategy of introduction. / Trabajo de investigación

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Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-12-15T20:22:31Z No. of bitstreams: 5 Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (1).pdf: 10525561 bytes, checksum: 07a8e4382df564fbf83da2a21bf32527 (MD5) Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (2).pdf: 10283132 bytes, checksum: 86891a8fb2cc6787c47e68abef54a9b7 (MD5) Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (3).pdf: 9957787 bytes, checksum: 8f10cc9b353e9438eb3f56aa2c31f367 (MD5) Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (4).pdf: 263110 bytes, checksum: 54511210b516c244f2746115cef2570f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Erika Demachki (erikademachki@gmail.com) on 2014-12-15T20:22:58Z (GMT) No. of bitstreams: 5 Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (1).pdf: 10525561 bytes, checksum: 07a8e4382df564fbf83da2a21bf32527 (MD5) Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (2).pdf: 10283132 bytes, checksum: 86891a8fb2cc6787c47e68abef54a9b7 (MD5) Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (3).pdf: 9957787 bytes, checksum: 8f10cc9b353e9438eb3f56aa2c31f367 (MD5) Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (4).pdf: 263110 bytes, checksum: 54511210b516c244f2746115cef2570f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-12-15T20:23:01Z (GMT). No. of bitstreams: 5 Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (1).pdf: 10525561 bytes, checksum: 07a8e4382df564fbf83da2a21bf32527 (MD5) Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (2).pdf: 10283132 bytes, checksum: 86891a8fb2cc6787c47e68abef54a9b7 (MD5) Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (3).pdf: 9957787 bytes, checksum: 8f10cc9b353e9438eb3f56aa2c31f367 (MD5) Dissertação - Elianne Auxiliadora Moreira Borges Munduruca - 2013 (4).pdf: 263110 bytes, checksum: 54511210b516c244f2746115cef2570f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-08-22 / Facade rendering perform various functions in buildings and monuments including aesthetic features, durability and economic value. However, over the years, the works begin to suffer deterioration processes, either natural or caused by unsuccessful intervention actions that trigger the onset of problems in the building rendering works, impairing these functions. In the case of historical buildings and monuments, the situation is even worse, because of the lack of knowledge of materials and techniques employed in implementing them at the time of construction. Thus, this thesis aims to study the most suitable methods for the inspection of works on facades and monuments of historical value and, from this research, aims to adapt a simplified methodology for the assessment of pathological manifestations of renderings, especially in art deco construction style built in Goiânia. To achieve this goal, was designed a case study in the Clock Tower, an important art deco monument of the capital of the Brazilian state of Goiás, has been proposed the following methodology: visual inspection for research and mapping of pathological manifestations present at the monument; photographic documentation; essay by percussion, to assess the state of rendering adhesion; application of the infrared thermograph technique; and also testing by pendulum sclerometry to evaluate the surface hardness of the rendering. As a result, it was found that the monument is deteriorated with the presence of various defects in the rendering, especially with a high cracking and dirt stains, and rust stains and disintegration. The south and north views of the tower are those most committed while in the west and east views, especially in the latter, the rendering is still in good condition. / Os revestimentos de fachadas exercem diversas funções nos edifícios e monumentos, entre as quais, funções estéticas, de durabilidade e de valorização econômica. Entretanto, com o passar dos anos, as obras começam a sofrer processos de deterioração, ora naturais ora provocados por ações de intervenção mal sucedidas, que desencadeiam o aparecimento de diversos problemas no revestimento, comprometendo essas funções. No caso dos edifícios e monumentos históricos, a situação é ainda pior, pois se somam a esses fatores, a falta de conhecimento dos materiais e das técnicas empregadas na execução deles na época da construção. Dessa forma, esta dissertação tem como objetivo estudar os métodos mais adequados para a inspeção em fachadas de obras e monumentos de valor histórico e, a partir dessa investigação, adaptar uma metodologia simplificada destinada à avaliação das manifestações patológicas dos revestimentos, especialmente de obras do estilo art déco construídas em Goiânia. Para o alcance desse objetivo, foi idealizado um estudo de caso na Torre do Relógio, importante monumento art déco da capital goiana, tendo sido proposta a seguinte metodologia: inspeção visual, para investigação e mapeamento das manifestações patológicas presentes no monumento; documentação fotográfica; ensaio por percussão, para avaliar o estado de aderência do revestimento; aplicação da técnica da termografia infravermelha e, ainda, o ensaio de esclerometria de pêndulo, para avaliar a dureza superficial do revestimento. Como resultado, constatou-se que o monumento encontra-se deteriorado, com a presença de diversas anomalias no revestimento, principalmente, com elevado índice de fissuração e manchas de sujidade, além de manchas de oxidação e desagregação. As vistas Sul e a Norte são as que estão mais comprometidas enquanto que nas vistas Oeste e Leste, especialmente nesta última, o revestimento ainda continua em bom estado de conservação.

O presente trabalho avaliou a efetividade do tratamento com tripsina (TT) na eliminação do BoHV-1 Colorado, causador da Rinotraqueíte Infecciosa Bovina (IBR). Camundongos fêmeas Swiss, com idade entre 6 e 8 semanas, foram superovuladas com 0,2mL a 5UI de hormônio eCG e, após 48h com hCG e acasaladas com machos inteiros da mesma linhagem e idade. Após 18 horas, as fêmeas foram eutanasiadas e, através de abertura no peritônio, os zigotos foram coletados. Foram separados zigotos para cinco ensaios que forneceram os seguintes dados: o BoHV-1 Colorado altera a taxa de clivagem e a morfologia do embrião; a tripsina, seguindo recomendações da IETS, não danifica o embrião; os embriões expostos aos vírus e submetidos ao TT não alteram sua morfologia e taxa de clivagem; foram detectados (nested-PCR+) vírus viáveis (MDBK+) após o TT. Estes resultados demonstram que o TT não foi efetivo para eliminar o BoHV-1 Colorado, porém em alguns ensaios o TT demonstrou eficiência na inativação do vírus o que torna-o um importante método de controle in vitro. / This study evaluated the effectiveness of treatment with trypsin (TT) in the elimination of BoHV-1 Colorado, which causes infectious bovine rhinotracheitis (IBR) Swiss female mice, aged 6 and 8 weeks, were superovulated with 0.2 mL 5UI hormone eCG and 48h after hCG and mated with males of the same lineage and age. After 18 hours, females were euthanized, and through na opening in the peritoneum, the zygotes were collected. Zygotes were separated for five trials that provided the following data: the Colorado BoHV-1 alters the cleavage rate and embryo morphology; trypsin, following recommendations of the IETS, does not damage the embryo; the embryos exposed to the virus and subjected to TT does not change their morphology and cleavage rate; were detected (nested-PCR +) viable virus (MDBK +) after the TT. These results demonstrate that TT was not effective to eliminate the BoHV-1 Colorado, but in some tests showed the TT efficiency in inactivating the virus makes it na important method of control in vitro.

O presente trabalho avaliou a efetividade do tratamento com tripsina (TT) na eliminação do BoHV-1 Colorado, causador da Rinotraqueíte Infecciosa Bovina (IBR). Camundongos fêmeas Swiss, com idade entre 6 e 8 semanas, foram superovuladas com 0,2mL a 5UI de hormônio eCG e, após 48h com hCG e acasaladas com machos inteiros da mesma linhagem e idade. Após 18 horas, as fêmeas foram eutanasiadas e, através de abertura no peritônio, os zigotos foram coletados. Foram separados zigotos para cinco ensaios que forneceram os seguintes dados: o BoHV-1 Colorado altera a taxa de clivagem e a morfologia do embrião; a tripsina, seguindo recomendações da IETS, não danifica o embrião; os embriões expostos aos vírus e submetidos ao TT não alteram sua morfologia e taxa de clivagem; foram detectados (nested-PCR+) vírus viáveis (MDBK+) após o TT. Estes resultados demonstram que o TT não foi efetivo para eliminar o BoHV-1 Colorado, porém em alguns ensaios o TT demonstrou eficiência na inativação do vírus o que torna-o um importante método de controle in vitro. / This study evaluated the effectiveness of treatment with trypsin (TT) in the elimination of BoHV-1 Colorado, which causes infectious bovine rhinotracheitis (IBR) Swiss female mice, aged 6 and 8 weeks, were superovulated with 0.2 mL 5UI hormone eCG and 48h after hCG and mated with males of the same lineage and age. After 18 hours, females were euthanized, and through na opening in the peritoneum, the zygotes were collected. Zygotes were separated for five trials that provided the following data: the Colorado BoHV-1 alters the cleavage rate and embryo morphology; trypsin, following recommendations of the IETS, does not damage the embryo; the embryos exposed to the virus and subjected to TT does not change their morphology and cleavage rate; were detected (nested-PCR +) viable virus (MDBK +) after the TT. These results demonstrate that TT was not effective to eliminate the BoHV-1 Colorado, but in some tests showed the TT efficiency in inactivating the virus makes it na important method of control in vitro.