Six wet pine flat sites were salvage logged following Hurricane Hugo in the fall of 1989. High soil moisture conditions during salvage operations resulted in soil compaction and deep rutting (puddling) on primary skid trails. Two studies were established to assess the effects of trafficking on soil physical properties and hydrologic characteristics. One addressed soil compaction; the other addressed soil rutting (puddling). Each study consisted of 3 sites (blocks) and trafficked and undisturbed areas were sampled on each site. Effects of trafficking on soil physical properties and hydrology were tested by comparing the disturbed (trafficked) and undisturbed subplots. Traffic increased bulk density and decreased soil porosity (macro-, micro-, total), water table levels, and saturated hydraulic conductivity within the compacted areas. However, the depth to reducing conditions was not significantly different from between undisturbed and disturbed areas of compacted sites. Puddling increased in bulk density and a decrease water table levels, depth of reducing conditions, soil porosity (macro-, micro-, and total), and saturated hydraulic conductivity (field and laboratory methods). Soil strength was not significantly different between disturbed and undisturbed areas. Overall, puddling resulted in soil changes that are more deleterious to tree growth than did compaction. Also puddling changes may prove more difficult to mitigate. / Master of Science

Wetlands provide a number of valuable functions to both the surrounding environment and society. The anaerobic conditions created by flooding in wetlands provide a habitat that supports unique assemblages of plant life. High altitude wetlands are amongst the most species-rich in South Africa. They house a number of rare species and play a vital role in the supply of water to lower lying areas. These are some of the reasons that mountain wetlands are of high conservation value. A phytosociological study was undertaken on the high altitude wetlands of the Great Escarpment with the aim of classifying the plant communities and identifying the environmental drivers of plant community patterns within these ecosystems. Data collection was focused in the Eastern Cape and was supplemented with data from existing studies to gain a more complete understanding of the wetlands of the Great Escarpment of South Africa. Using the Braun-Blanquet approach, Hierarchical Cluster Analysis and Indicator Species Analysis; five broad wetland groups were identified, comprised of 33 individual plant communities and 81 indicator species. Multivariate analysis, including Canonical Correspondence Analysis revealed that the effects of altitude, such as temperature and rainfall, are the most significant large-scale drivers of vegetation patterns. Smaller scale drivers include wetness and soil nutrients including nitrogen, phosphorus, electrical conductivity, sodium, and organic content. The identification of indicator species served to reveal potentially important wetland species across different areas of the Great Escarpment. The effects of altitude on plant community patterns highlights the susceptibility of the high altitude specific communities to upward temperature zone shifts resulting from global warming. Other threats include livestock trampling, water extraction, and land use change for agricultural purposes. The relative absence of alien species in these wetlands gives an indication of their pristine condition and therefore their importance as a reference from which they may be monitored. A large proportion of the wetlands studied here occur outside protected areas, and given the rate of wetland loss in South Africa, it is important that continued effective land management is practiced to ensure that these ecosystems are conserved in the future .

New knowledge gained over the past 15 to 20 years on the management of riparian zones in Arizona for water yield improvement has been organized and analyzed. Hydrologic processes and principles applicable to riparian zones, the distribution and nature of riparian vegetation in Arizona, and new resource management methods, needs, and constraints have been evaluated, The relationship between vegetation management for water yield improvement and other resource based products and uses of riparian zones such as timber, range for livestock, wildlife and fish, recreation, and aesthetics was also assessed. Past studies and surveys indicate that Arizona has approximately 280,000 to 320,000 acres of riparian vegetation, However, pertinent information such as species composition, vegetation density, depth to groundwater, groundwater quality, and landownership have not been mapped accurately for many riparian zones in Arizona, A continuous survey of riparian vegetation cover by remote sensing supplemented by ground truth is suggested to remedy this situation. Analysis of hydrologic studies indicated the following identifiable trends in water use by riparian species, Saltcedar, arrowweed, cottonwood, and hydrophytes are the heaviest users of water (between four and eight feet of water annually). Intermediate water users (annual use between two and five feet) are seepwillow, mesquite, quailbrush, four-wing saltbush, and greasewood. Lesser amounts of water are transpired by grasses and sedges and evaporated from bare soil (0,5 to three feet annually). Five water yield improvement methods applicable to riparian zones are evaluated; conversion of one vegetation type to another, channelization, cottonwood thinning, antitranspirant and biological control treatments. Conversion treatments to grasses or crops may yield water savings of up to 2,5 acre-feet per acre annually during the first year, However, some or all of this water may eventually be used by replacement vegetation, Several constraints including possible loss of wildlife habitat, contamination of water supplies by chemical herbicides, lowered aesthetic quality, and increased soil erosion with the removal of riparian vegetation reduce the opportunities for converting a large percentage of riparian vegetation in Arizona, To justify operational conversion programs in Arizona follow up studies of current conversion projects should be instituted, Rates at which revegetation takes place, declines in water salvage as revegetation occurs, amount and value of increased herbage production, and long term effects on plant distribution and animal life need to be determined. Channelization projects in the Southwest have been credited with increasing water yields; however, methods for determining these increases are poorly documented. Channelization for flood control purposes is limited because flow of flood water is accelerated in the vicinity of the excavation and may contribute to flooding and sedimentation on unchanneled segments. Cottonwood thinning designed to reduce evapotranspiration and flood hazards has been conducted along the Verde River, Increased water yields have not been measured, Adverse effects on fish and wildlife have been reported as a result of thinning cottonwoods. Limited thinning of cottonwoods to prevent bridges from washing out or to protect existing structures on the floodplain may be beneficial. Application of antitranspirant foliar sprays to reduce plant water use is a potential treatment method for increasing water yield in riparian zones. Antitranspirants were effective in reducing transpiration rates of saltcedar plants by up to 38 per cent for three to five weeks in greenhouse and limited field studies, Research on the feasibility of obtaining supplementary water from riparian vegetation through the application of antitranspirants should be expanded, Antitranspirants, if proven safe and effective, may be mutually acceptable to water, recreation, and wildlife interests. Biological control of saltcedar is not effective at present.

Submitted in complete fulfillment for the degree of Doctor of Philosophy (Biotechnology) in the Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa, 2016. / Many households in developing countries are still without proper sanitation systems. The problems are even more prevalent in rural communities where there are no septic systems in place for the treatment of wastewater. This has resulted in the urgent need for the development and implementation of innovative wastewater treatment systems that are inexpensive, environmental friendly and are able to reduce contaminants to levels that pose no harm to the communities. Constructed rhizofiltration systems have been explored for this purpose. They have been used for many decades in many countries with varying degrees of success at the primary, secondary and tertiary levels of wastewater treatment. Poor optimization of this technology has been due to limited information available about the roles played by the whole system as well as by each component involved in the treatment technology. The current work elucidates the role played by macrophytes and microbial biofilms in the removal of nutrients in the rhizofiltration system. Factors affecting waste removal as well as environmental friendliness of the system were also investigated. The rhizofiltration system was constructed in Durban and was divided into planted (planted with Phragmites australis and Kyllinga nemoralis) and unplanted (reference) section. Dissolved oxygen (DO), pH, water temperature, total dissolved solids (TDS), electrical conductivity (EC) and salinity were monitored. The removal efficiency of nutrients was measured using spectrophotometric methods by measuring the concentration of ammonia, nitrate, nitrite, phosphate and orthophosphate in the wastewater pre- and post-treatment. The total organic carbon, chemical oxygen demand (COD), total Kehldjahl nitrogen, biological oxygen demand (BOD), ammonia, nitrate and the flow rate of wastewater into the system from the settling tank were used for the estimation of carbon dioxide, methane and nitrous oxide emitted from the rhizofilter using the 2009 EPA formulae. Both the planted and reference sections of the system removed nutrients with varying efficiencies. The reduction of nutrients in the rhizofilter was found to be seasonal, with most nutrients removed during the warm seasons. The system also retained more nutrients when wastewater containing low levels of nutrients was used. The unpaired t-test was used to determine the differences between nutrient removals between planted and reference sections. Higher reduction efficiencies of nutrients were obtained in the planted section. Up to 65% nitrite and 99% nitrate were removed while up to 86% total phosphorus was removed in a form of orthophosphate (86%). Removal of total nitrogen was shown to increase under high temperature conditions, while the same conditions decreased the total phosphorus removal. High temperatures also increased the performance of the system. The reduction of nutrients in the system corresponded to reduction of the chemical oxygen demand which also positively correlated to the dissolved oxygen concentration. Considering the discharge limits for all nutrients, the discharges in the effluent of the planted section were within the allowable limits as per South Africa’s Department of Water affairs and Forestry in 2012 but not in 2013. The results obtained in 2013 were due to increased nutrient loading introduced into the system. Diverse microbial communities occurred in the treatment system, with more diversity in the planted section. These organisms were supported by macrophytes in the planted section, and were responsible for nitrogen and phosphorus transformation. This explains why total nitrogen and phosphorus reduction was higher in the planted compared to the reference section. Both the planted and the reference sections of the rhizofiltration system produced the greenhouse gases. When the two sections were compared, the planted section produced more gases. Gases emitted by both sections were lower when compared to emission from sludge treatment reed beds and other conventional systems of wastewater treatments. These findings indicated that constructed rhizofiltration is a cleaner form of waste treatment, producing significantly less greenhouse gases and affecting less of a climate change. Findings of this work have revealed that rhizofiltration technology can be used as a low-cost alternative technology for the treatment of wastewater, using the combination of macrophytes and microbial biofilms. Macrophytes accumulated nitrogen and phosphorus as well as supported diverse microorganisms that metabolized and reduced nutrients in the rhizofiltration unit. / D

Abstract____________________________________________________________________ 2 ABSTRACT: Zea mays is generally considered to be a plant with inducible lysigenous aerenchyma formation. The degradation of some cortical cells is triggered by environmental conditions, usually in the form of stress (submergence etc.). These cells die in a process that shows signs characteristic for programmed cell death, such as nuclear DNA fragmentation or apoptotic ultrastructural alterations. Aerenchyma formed in primary roots of thirteen examined maize accessions, irrespective of cultivation conditions. The aerenchyma fraction correlated with the root length, not with its age. The dependence of aerenchyma formation on the presence of this phytohormone was proved by using an inhibitor of ethylene synthesis (AOA). It was found out that the aerenchyma formation depended on light conditions and that the lysigenous intercellulars intercellular spaces colocalized with areas with cells with characteristically fragmented nuclear DNA (TUNEL-positive nuclei). In experiments using the TUNEL reaction it was necessary to determine new dilution of the enzymatic mixture for the examined plant material. Only the observation of surface planes of free-hand root sections was considered relevant in both TUNEL-TMR and TUNEL-AP assays. TUNEL-AP...

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Includes bibliography. / 2 v. : ill., maps ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This thesis analyses the aquatic macrophyte vegetation of remnant wetlands in the South East of South Australia and of Bool Lagoon in particular. The study describes the diversity of aquatic vegetation in 11 remnant and ephemeral wetlands in the South East using a multivariate approach of classification and ordination of vegetation quadrats. The second section of the study characterises the seasonal pattern of fluctuation through a numerical description of water regime. The third section is a consideration of the population demography of Typha domingensis and Phragmites australis in relation to flooding frequency at Bool Lagoon. The final section of the study considers the influence of flooding frequency on the distribution of some important components of the vegetation of Bool Lagoon. / Thesis (Ph.D.)--University of Adelaide, Dept. of Botany, 1997?

Thesis (Ph.D.) -- Dept. of Botany, University of Adelaide, 1993. / Bibliography: leaves 103-120.

Thesis (M. Phil.)--University of Hong Kong, 1998. / Includes bibliographical references (leaves 129-138).

Thesis (Ph.D.)--University of Washington, 1995. / Vita. Includes bibliographical references (leaves [135]-151).