The study was conducted in the Greater Kruger National Park Complex (GKNP), which consists of the Kruger National Park (KNP) and adjacent private game reserves and focuses primarily on the following objectives: • To monitor and evaluate the standard of the existing disease surveillance programmes for the following diseases, Foot and Mouth, Anthrax, Tuberculosis, Brucellosis and Rabies, within the Kruger National Park and adjacent private game reserves by evaluating the level of competency and knowledge in field rangers, field guides and trails rangers with regard to these specific diseases. It can be stated that important differences exist between disease surveillance techniques used for domestic animals and those used for wildlife (Bengis, R.G., Kock, R.A., & Fischer, J., 2002). According to Morner, T., Obendorf, D.L., Artios, M., & Woodford, M.H., 2002, it is more difficult to monitor diseases in wildlife than in domestic animals because wild animals are not constrained by boundaries and can roam over large. distances. For significant diseases in wildlife, an active surveillance programme may be the preferred approach with the aim to collect a certain number of samples from a target population (live or dead animals) to determine the point prevalence of certain pathogens. Active veterinary participation is essential in protected area management, with emphasis on training of technicians, rangers and field biologists with regard to specific diseases and their clinical signs, surveillance and sampling techniques, data collection, and reporting. For the purpose of this study, data collection was conducted by means of a questionnaire drawn up according to the related critical points as described in the Dufour grid (Dufour, 1998). The results of this study clearly showed a need to address certain important aspects regarding a wildlife disease programme within the GKNP. A more efficient wildlife disease surveillance programme, which included more specific and “hands-on” trained staff, would definitely ensure a better early warning system which would detect new or emerging disease outbreaks. / M. Tech. (Nature Conservation))

The study was conducted in the Greater Kruger National Park Complex (GKNP), which consists of the Kruger National Park (KNP) and adjacent private game reserves and focuses primarily on the following objectives: • To monitor and evaluate the standard of the existing disease surveillance programmes for the following diseases, Foot and Mouth, Anthrax, Tuberculosis, Brucellosis and Rabies, within the Kruger National Park and adjacent private game reserves by evaluating the level of competency and knowledge in field rangers, field guides and trails rangers with regard to these specific diseases. It can be stated that important differences exist between disease surveillance techniques used for domestic animals and those used for wildlife (Bengis, R.G., Kock, R.A., & Fischer, J., 2002). According to Morner, T., Obendorf, D.L., Artios, M., & Woodford, M.H., 2002, it is more difficult to monitor diseases in wildlife than in domestic animals because wild animals are not constrained by boundaries and can roam over large. distances. For significant diseases in wildlife, an active surveillance programme may be the preferred approach with the aim to collect a certain number of samples from a target population (live or dead animals) to determine the point prevalence of certain pathogens. Active veterinary participation is essential in protected area management, with emphasis on training of technicians, rangers and field biologists with regard to specific diseases and their clinical signs, surveillance and sampling techniques, data collection, and reporting. For the purpose of this study, data collection was conducted by means of a questionnaire drawn up according to the related critical points as described in the Dufour grid (Dufour, 1998). The results of this study clearly showed a need to address certain important aspects regarding a wildlife disease programme within the GKNP. A more efficient wildlife disease surveillance programme, which included more specific and “hands-on” trained staff, would definitely ensure a better early warning system which would detect new or emerging disease outbreaks. / M. Tech. (Nature Conservation))

Leopards (Panthera pardus) are the most common large predators, free roaming outside of protected areas across most of South Africa. Leopard persistence is attributed to their tolerance of rugged terrain that is subject to less development pressure, as well as their cryptic behaviour. Nevertheless, existing leopard populations are threatened indirectly by ongoing transformation of natural habitat and directly through hunting and conflict with livestock farmers. Together these threats may further isolate leopards to fragmented areas of core natural habitat. I studied leopard habitat preferences, population density, diet and the attitudes of landowners towards leopards in the Little Karoo, Western Cape, South Africa, an area of mixed land-use that contains elements of three overlapping global biodiversity hotspots. Data were gathered between 2010 and 2012 using camera traps set up at 141 sites over an area of ~3100km², GPS tracking collars fitted to three male leopards, scat samples (n=76), interviews with landowners (n=53) analysed in combination with geographical information system (GIS) layers. My results reveal that leopards preferred rugged, mountainous terrain of intermediate elevation, avoiding low-lying, open areas where human disturbance was generally greater. Despite relatively un-fragmented habitat within my study area, the leopard population density (0.75 leopards/100km²) was one of the lowest yet recorded in South Africa. This may reflect low prey densities in mountain refuges in addition to historical human persecution in the area. Currently local landowners are more tolerant of leopards than other wildlife species with incidents of conflict involving leopards being rare relative to black-backed jackals (Canis mesomelas), baboons (Papio hamadryas), caracals (Caracal caracal) and porcupine (Hystrix africaeaustralis). Although current levels of conflict between leopards and stock farmers are low, leopards do depredate livestock, which constitute 10-15% of their diet. Improved livestock husbandry measures and co-operation between conservation authorities and farmers are necessary to mitigate such conflict and balance economic security with biodiversity conservation in the region. Leopards are the only remaining top predators throughout much of the Little Karoo and the Western Cape and as such are predicted to play a critical role in ecosystem structure and the survival of other species. Current high levels of connectivity between areas of suitable leopard habitat bode well for the conservation status of leopards within this region and future conservation efforts need to ensure that narrow corridors linking such habitat are preserved. The potential for leopards to serve as both an umbrella and a flagship species for biodiversity conservation suggests that long term monitoring of this population would be a conservation priority for the Little Karoo.

The conservation of leopards in the Eastern Cape Province requires a holistic approach that considers both predator-human interactions as well as the biology of the carnivore. Numerous studies have been conducted on leopards within protected areas; however more information regarding the species is needed outside these areas to facilitate effective management of predators. The spatial ecology of the leopard (Panthera pardus) were studied in the Baviaanskloof and GAENP in the Eastern Cape. The Baviaanskloof is an extensive area of mountainous terrain (approximately 2665km²) which has a mosaic of land uses, and leopards move from conservation areas to farmland where they come into contact and conflict with farmers. This study examined the spatial ecology of leopards living on farmlands adjacent to protected land. The space utilization and activity patterns of six leopards were analysed. These animals were caught and released on farmlands in the Baviaanskloof (n=4) or translocated (n=2) when not possible to release on site. The animals were caught by means of fall-door, walk-in traps and fitted with Vectronic GPS collars that facilitated the collection of high quality GPS fixes from each animal. Data was collected using VHF and UHF telemetry to download data. An understanding of spatial requirements in areas with different land use, and the extent of overlap of space use with other leopards allow, for the first time in the region, the calculation of possible maximum population size. Analysis of range size was carried out using two methods: minimum convex polygon, and Kernel Utilization Distribution. Finally, a key predictor of space use is prey availability. I assessed the prey base using a grid of camera traps. The studied leopards revealed large range utilization with minimal overlap. The activity patterns suggest there is no preference between diurnal and nocturnal activity patterns and the population density was estimated between 0.3 - 1.3 leopards per 100km². Large home range sizes and low population densities suggest that leopards require large areas of suitable habitat, and that conservation efforts need to be extended beyond protected areas to ensure the long-term viability of leopard populations in such areas.