published_or_final_version / Community Medicine / Master / Master of Philosophy

Tse, Ka Yan Agnes. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2009. / Includes bibliographical references (leaves 106-113). / Abstract also in Chinese. / Table of Contents --- p.i / List of Figures and Tables --- p.iii / List of Abbreviations --- p.vi / Chapter Chapter 1: --- Introduction --- p.1 / Chapter 1.1 --- Breast cancer overview --- p.1 / Chapter 1.2 --- General mechanism of breast carcinoma --- p.5 / Chapter 1.3 --- Ductal carcinoma in situ (DCIS) --- p.7 / Chapter 1.3.1 --- Clinical features of DCIS --- p.7 / Chapter 1.3.2 --- Classification of DCIS --- p.8 / Chapter 1.3.3 --- Molecular markers for DCIS --- p.14 / Chapter 1.3.4 --- Progression of DCIS --- p.19 / Chapter 1.4 --- Aim of project --- p.20 / Chapter Chapter 2: --- Materials and Methods --- p.21 / Chapter 2.1 --- Categorization of DCIS samples using Van Nuys Grading System --- p.21 / Chapter 2.2 --- "RNA extraction and reverse transcription of formalin-fixed, paraffin embedded (FFPE) DCIS samples" --- p.22 / Chapter 2.2.1 --- "Formalin-fixed, paraffin embedded (FFPE) DCIS sample" --- p.22 / Chapter 2.2.2 --- Tissue Microarray (TMA) --- p.24 / Chapter 2.2.3 --- RNA extraction --- p.25 / Chapter 2.2.4 --- First strand cDNA synthesis --- p.30 / Chapter 2.3 --- PCR screening for potential DCIS markers --- p.31 / Chapter 2.4 --- Statistical analysis of PCR expression pattern --- p.36 / Chapter 2.4.1 --- Chi-square lest --- p.36 / Chapter 2.4.2 --- Logistic regression --- p.37 / Chapter 2.5 --- Real-time RT-PCR analysis of the expression pattern of the potential DCIS marker --- p.39 / Chapter 2.5.1 --- Real-time RT-PCR for FFPE samples --- p.39 / Chapter 2.5.2 --- Real-time RT-PCR in the study --- p.40 / Chapter 2.6 --- Statistical analysis of real-time RT-PCR results --- p.41 / Chapter 2.7 --- Immunohistological analysis of the expression pattern of the potential DCIS marker --- p.42 / Chapter 2.8 --- Evaluation of immunohistological staining --- p.44 / Chapter 2.9 --- Statistical analysis of immunohistological results --- p.44 / Chapter Chapter 3: --- Analysis of expression pattern of potential DCIS markers --- p.45 / Chapter 3.1 --- Construction of DCIS data base from FFPE samples --- p.45 / Chapter 3.2 --- Analysis of expression of potential DCIS marker from PCR --- p.46 / Chapter 3.2.1 --- First strand cDNA synthesis and PCR screening --- p.46 / Chapter 3.2.2 --- Statistical analysis of PCR results --- p.60 / Chapter 3.3 --- Conclusion --- p.66 / Chapter Chapter 4: --- Real-time RT-PCR analysis of expression of selected potential DCIS markers --- p.67 / Chapter 4.1 --- Expression of ETV6 in tumor and adjacent normal tissues in different DCIS grades --- p.69 / Chapter 4.2 --- Expression of Erbb2 in tumor and adjacent normal tissues in different DCIS grades --- p.71 / Chapter Chapter 5: --- Analysis of Protein Expression Pattern of Potential DCIS Markers --- p.73 / Chapter 5.1 --- Chi square test --- p.73 / Chapter 5.1.1 --- Immunohistochemical study of ETV6 in tumor and adjacent normal DCIS --- p.74 / Chapter 5.1.2 --- Immunohistochemical study of Erbb2 in tumor and adjacent normal DCIS tissues --- p.77 / Chapter 5.2 --- Conclusion --- p.80 / Chapter Chapter 6: --- General discussion --- p.81 / References --- p.106

Abstract not available