Orientador: Fabio Porto-Foresti / Resumo: The billfish are oceanic pelagic species often caught by the tuna fleet and with great interest of sport fishing. This group of fish has specific legislation prohibiting the marketing and export of two species, Kajuikia albida e Makaira nigricans, in Brazil. DNA barcode is a universal system of molecular identification based on a sequence of mitochondrial DNA cytochrome oxidase subunit I (COI) of about 650 base pairs, serving as an identifying genomic mark in each species. This system has been used successfully to identify mischaracterized samples illegally marketed, like sample materials from sharks and other endangered species of organisms. The Barcode DNA technique was used to identify billfish marketed in the second largest fishing warehouse in Latin America, the CEAGESP-SP and the international airport of São Paulo, Brazil. 302 samples of billfish were collected during three inspection actions carried out by IBAMA, the executing agency of the national environmental policy. After DNA sequencing procedures it was possible to identify 72 samples (91.14%) by CEAGESP at the level of species, being 21 (29.17%) identified as Xiphias gladius, 44 (61.1%) as Istiophorus platypterus and 7 (8.33%) as Kajikia albida, this latter species determined as a market prohibited in Brazil and considered vulnerable in the list of endangered species of the IUCN and in the official list of species of endangered Fauna - Fish and Aquatic Invertebrates. At the International Airport of Guarulhos it ... (Resumo completo, clicar acesso eletrônico abaixo) / Mestre

Submitted by CARLOS EGBERTO RODRIGUES JÚNIOR null (egberto_ibama@hotmail.com) on 2017-09-27T13:42:52Z No. of bitstreams: 1 Dissertação Carlos Egberto Rodrigues Junior repositorio.pdf: 4590386 bytes, checksum: 2cd3b020266ae2b4f501ad0d4b2ba74a (MD5) / Rejected by Monique Sasaki (sayumi_sasaki@hotmail.com), reason: Solicitamos que realize uma nova submissão seguindo a orientação abaixo: O arquivo submetido está sem a ficha catalográfica. A versão submetida por você é considerada a versão final da dissertação/tese, portanto não poderá ocorrer qualquer alteração em seu conteúdo após a aprovação. Corrija esta informação e realize uma nova submissão contendo o arquivo correto. Agradecemos a compreensão. on 2017-09-28T14:11:18Z (GMT) / Submitted by CARLOS EGBERTO RODRIGUES JÚNIOR null (egberto_ibama@hotmail.com) on 2017-11-01T11:03:15Z No. of bitstreams: 1 Dissertação Carlos Egberto Rodrigues Junior repositorio.pdf: 4606348 bytes, checksum: c20c3df76e489701ead450f3ca92dcad (MD5) / Approved for entry into archive by LUIZA DE MENEZES ROMANETTO (luizamenezes@reitoria.unesp.br) on 2017-11-13T15:03:34Z (GMT) No. of bitstreams: 1 rodriguesjunior_cer_me_bot.pdf: 4606348 bytes, checksum: c20c3df76e489701ead450f3ca92dcad (MD5) / Made available in DSpace on 2017-11-13T15:03:34Z (GMT). No. of bitstreams: 1 rodriguesjunior_cer_me_bot.pdf: 4606348 bytes, checksum: c20c3df76e489701ead450f3ca92dcad (MD5) Previous issue date: 2016-12-15 / The billfish are oceanic pelagic species often caught by the tuna fleet and with great interest of sport fishing. This group of fish has specific legislation prohibiting the marketing and export of two species, Kajuikia albida e Makaira nigricans, in Brazil. DNA barcode is a universal system of molecular identification based on a sequence of mitochondrial DNA cytochrome oxidase subunit I (COI) of about 650 base pairs, serving as an identifying genomic mark in each species. This system has been used successfully to identify mischaracterized samples illegally marketed, like sample materials from sharks and other endangered species of organisms. The Barcode DNA technique was used to identify billfish marketed in the second largest fishing warehouse in Latin America, the CEAGESP-SP and the international airport of São Paulo, Brazil. 302 samples of billfish were collected during three inspection actions carried out by IBAMA, the executing agency of the national environmental policy. After DNA sequencing procedures it was possible to identify 72 samples (91.14%) by CEAGESP at the level of species, being 21 (29.17%) identified as Xiphias gladius, 44 (61.1%) as Istiophorus platypterus and 7 (8.33%) as Kajikia albida, this latter species determined as a market prohibited in Brazil and considered vulnerable in the list of endangered species of the IUCN and in the official list of species of endangered Fauna - Fish and Aquatic Invertebrates. At the International Airport of Guarulhos it was possible to identify 223 samples (100%), all identified as Xiphias gladius as declared by the exporter and indicated by its NCM (Mercosur Common Nomenclature). Molecular analysis has proven to be an excellent tool to identify billfish in Brazil in different centers such as Guarulhos International Airport and CEAGESP, SP. The identification of the white marlin being illegally traded in CEAGESP demonstrates the ineffectiveness of the current monitoring techniques used and emphasize the need for adopting better public policies for the conservation of the species. / Os peixes de bico são espécies pelágicas oceânicas frequentemente capturadas pela frota atuneira e com grande interesse da pesca esportiva. Este grupo de peixes possui legislação específica que proíbe a comercialização e exportação de duas espécies, Kajikia albida e Makaira nigricans desse grupo no Brasil. O DNA Barcode é um sistema universal de identificação molecular baseado em uma sequência de DNA mitocondrial Citocromo Oxidase subunidade I (COI) de aproximadamente 650 pares de base, servindo como código de barras de DNA de cada espécie. Este sistema tem sido utilizado com êxito para identificar amostras que são comercializadas descaracterizadas e de forma ilegal, como tubarões e outras espécies ameaçadas de extinção. A técnica de DNA Barcode foi utilizada para a identificação de peixes de bico comercializados no segundo maior entreposto pesqueiro da América Latina, CEAGESP, SP e no Aeroporto Internacional de Guarulhos, atualmente o maior volume brasileiro de exportações de peixes de bico no País. Foram coletadas 302 amostras de peixes de bico durante vistorias realizadas pelo IBAMA, 223 no aeroporto e 79 no CEAGESP, órgão executor da política nacional do meio ambiente. Após sequenciamento do DNA foi possível à identificação das espécies de 70 amostras (88,60%) coletadas no CEAGESP, sendo 21 (30%) identificadas como Xiphias gladius, 43 (61,42%) como Istiophorus platypterus e seis (8,57%) como Kajikia albida, esta última de comércio proibido no Brasil, e considerada vulnerável pela lista de espécies em perigo da IUCN e pela Lista Nacional Oficial de Espécies da Fauna Ameaçadas de Extinção - Peixes e Invertebrados Aquáticos. No Aeroporto Internacional de Guarulhos foi possível à identificação de 223 amostras (100%), sendo todas identificadas como Xiphias gladius conforme declarado pelo exportador e indicado pelo seu NCM (Nomenclatura Comum do Mercosul). A análise molecular demonstrou ser uma importante ferramenta na identificação de peixes de bico comercializados no Brasil em diferentes centros como o Aeroporto Internacional de Guarulhos e CEAGESP, SP. A identificação de agulhões branco sendo ilegalmente comercializados na CEAGESP demonstra a ineficácia das técnicas de fiscalização utilizadas e enfatizam a necessidade melhores políticas públicas para a conservação da espécie.

Dolphinfish (Coryphaena hippurus) is a pelagic species that is ecologically and commercially important in the western Atlantic region. This species has been linked to dominant oceanographic features such as sea surface temperature (SST) frontal regions. This work first explored the linkages between the catch rates of dolphinfish and the oceanography (satellite-derived SST, distance to front calculations, bottom depth and hook depth) using Principal Components Analysis (PCA). It was demonstrated that higher catch rates are found in relation to warmer SST and nearer to frontal regions. This environmental information was then included in standardizations of catch-per-unit-effort (CPUE) indices. It was found that including the satellite-derived SST and distance to front increases the confidence in the index. The second part of this work focused on addressing spatial variability in the catch rate data for a subsection of the sampling area: the Gulf of Mexico region. This study used geostatistical techniques to model and predict spatial abundances of two pelagic species with different habitat utilization patterns: dolphinfish (Coryphaena hippurus) and swordfish (Xiphias gladius). We partitioned catch rates into two components, the probability of encounter, and the abundance, given a positive encounter. We obtained separate variograms and kriged predictions for each component and combined them to give a single density estimate with corresponding variance. By using this two stage approach we were able to detect patterns of spatial autocorrelation that had distinct differences between the two species, likely due to differences in vertical habitat utilization. The patchy distribution of many living resources necessitates a two-stage variogram modeling and prediction process where the probability of encounter and the positive observations are modeled and predicted separately. Such a "geostatistical delta-lognormal" approach to modeling spatial autocorrelation has distinct advantages in allowing the probability of encounter and the abundance, given an encounter to possess separate patterns of autocorrelation and in modeling of severely non-normally distributed data that is plagued by zeros.

A diet study of swordfish, Xiphias gladius, was conducted in the Straits of Florida from April 2007 to December 2008. The stomachs of 131 swordfish were analyzed. Thirteen species of teleosts, three species of cephalopods, and one species of crustacean were observed in the diet. Cephalopods dominated the swordfish diet by weight (73.38%), number (69.90%), and occurrence (80.91%) and ranked highest in importance in the diet when calculating the index of relative importance (IRI). Teleosts followed by weight (25.16%), number (26.34%), occurrence (68.18%), and IRI (3,510.97). The prey species with the greatest dietary importance was Illex sp followed by unidentifiable ommastrephids. Stomach fullness index (SFI) was calculated ranging from 0 (empty stomachs) to 3.57. Additionally, correlations for juvenile male swordfish length and prey length (r²=0.647) as well as female adult swordfish weight and prey weight (r²=-0.327) were found to be significant. Overall feeding seasonality showed that swordfish primarily fed on teleosts (58%) in the spring, cephalopods (70%) in the summer, and cephalopods (75%) in the winter. When considered individually, female, male, and adult swordfish followed this pattern; however juveniles did not follow the overall feeding trend according to season. They fed regularly on cephalopods during spring (57%) and summer (61%) consuming the highest abundance of cephalopods during the winter (85%). Overall, swordfish appeared to be opportunistic feeders altering its food choices when abundance of prey may have changed or when food items were present.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-07T12:40:31Z No. of bitstreams: 1 Ana Lia Camposa Quaggio.pdf: 1430515 bytes, checksum: 68e33f51877fa5eba2d7e0997b3e6520 (MD5) / Made available in DSpace on 2017-02-07T12:40:31Z (GMT). No. of bitstreams: 1 Ana Lia Camposa Quaggio.pdf: 1430515 bytes, checksum: 68e33f51877fa5eba2d7e0997b3e6520 (MD5) Previous issue date: 2010-02-08 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Since the 50’s, Xiphias gladius (Linnaeus, 1758) has been captured along the Brazilian coast by surface longliners. This is the second most important target species for the tuna fleet based in Itajaí, Santa Catarina State, after the blue-shark, Prionace glauca (Linnaeus, 1758). The study was based on data from sea observers, log books and carcass ladings of Xiphias gladius, Linnaeus, 1758) from the pelagic longline fleet based in Itajaí, which operated in the southwest Atlantic. Covariance models were fit to the catches (weight, kg) of swordfish and the effects used were: (1) the years 1997, 1998, 2001, 2002 e 2007 (2) lunar phases and (3) seasons. The covariate was fishing effort (hooks). The highest moon catches occurred on the first quarter moon and during wintertime. The Mantel-Haenszel procedure was used to test the hook selectivity and showed that the type “J” hook (J 9/0 10º offset), traditionally used by the fleet, have 1.2 more chances of catching Xiphias gladius than the “circle” hook (18/0 10º offset), used as an optinal device to reduce sea turtle bycatch. The M-H test showed that the J hook has 1,2 more chances of catching Xiphias gladius than the circle hook. The conversion of individual carcass weights (kg) to total length (cm) for the years 2000 to 2002 showed that 66% of the catches were sub-adults. / O espadarte, Xiphias gladius (Linnaeus, 1758), vem sendo capturado na costa brasileira desde os anos 50 pelo espinhel-de-superfície. Esta é a segunda mais importante espécie-alvo da frota atuneira sediada em Itajaí (SC), ficando atrás apenas do tubarão-azul, Prionace glauca (Linnaeus, 1758). No presente estudo foram analisados os dados obtidos para esta espécie através de observadores, mapas de bordo e fichas de desembarque de carcaças de X. gladius para a frota de espinhel-de-superfície sediada em Itajaí (SC) e atuante no Atlântico sudoeste. Foram elaborados modelos de análise de covariância (ANCOVA) e aplicados aos dados de captura (peso em kg) do espadarte, tendo como efeitos: (1) os anos de 1997, 1998, 2001, 2002 e 2007 (2) as fases lunares e (3) as épocas do ano. A covariável foi o esforço de pesca (n° de anzóis). As maiores médias de captura ocorreram durante as fases de lua crescente e no inverno. O procedimento estatístico de Mantel-Haenszel teste foi utilizado para comparar as capturas do espadarte no anzol tipo “Jota” (9/0 10º offset) comumente utilizado pela frota espinheleira com o anzol tipo “circular” (18/0 10ºoffset), este último oferecido como alternativa mitigadora à captura de tartarugas marinhas. O teste M-H indicou que o anzol “Jota” apresentou 1,2 mais chances de capturar Xiphias gladius que no “circular”. A conversão dos pesos individuais das carcaças (kg) em comprimentos totais (cm), referentes aos anos de 2000 a 2002, indicou que 66% das capturas foram de sub-adultos, ao longo de todo o ano.