Lyme disease has been a growing problem in the United States over the last few decades, and is currently the most common vector-borne disease in the country. This research evaluates the land cover within specified counties of northern Virginia to determine if a correlation exists between forest fragmentation, suburbanization, and cases of human Lyme disease as has been demonstrated in other Lyme endemic regions in the United States. Few studies have focused specifically on northern Virginia when considering the impacts of land cover change on Lyme disease. Discovered through the use of geospatial and statistical analysis, the cluster of Lyme disease cases in northern Virginia are associated with forest fragmentation within the study region, which creates an ideal habitat for black-legged ticks and the white-footed mouse, allowing for an increase in Lyme disease transfer from vector to humans. The goal is for the research findings to be applicable to other regions with similar land cover types. Regions with similar characteristics would then be able to recognize the potential risk of human Lyme disease and implement ways to reduce the Lyme disease risk associated with suburban development. The purpose of this study is to answer the following research questions: 1) How has the spatial distribution of Lyme disease in Northern Virginia changed since 2005 with respect to land cover? 2) Which suburban communities are more at risk for Lyme disease when considering their land cover types and the increasing spatial distribution of Lyme disease? / Master of Science / Lyme disease has been a growing problem in the United States over the last few decades, and is currently the most common vector-borne disease in the country. This research evaluates the land cover within specified counties of northern Virginia to determine if a correlation exists between forest fragmentation, suburbanization, and cases of human Lyme disease as has been demonstrated in other Lyme endemic regions in the United States. Few studies have focused specifically on northern Virginia when considering the impacts of land cover change on Lyme disease. Discovered through the use of geospatial and statistical analysis, the cluster of Lyme disease cases in northern Virginia are associated with forest fragmentation within the study region, which creates an ideal habitat for black-legged ticks and the white-footed mouse, allowing for an increase in Lyme disease transfer from vector to humans. The goal is for the research findings to be applicable to other regions with similar land cover types. Regions with similar characteristics would then be able to recognize the potential risk of human Lyme disease and implement ways to reduce the Lyme disease risk associated with suburban development. The purpose of this study is to answer the following research questions: 1) How has the spatial distribution of Lyme disease in Northern Virginia changed since 2005 with respect to land cover? 2) Which suburban communities are more at risk for Lyme disease when considering their land cover types and the increasing spatial distribution of Lyme disease?