Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-04-08T23:56:19Z No. of bitstreams: 1 JoseRodolfoLopesDePaivaCavalcanti_TESE.pdf: 2156602 bytes, checksum: 000c3eafedc8a66a1a55425b0168fd4a (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-04-11T23:46:45Z (GMT) No. of bitstreams: 1 JoseRodolfoLopesDePaivaCavalcanti_TESE.pdf: 2156602 bytes, checksum: 000c3eafedc8a66a1a55425b0168fd4a (MD5) / Made available in DSpace on 2016-04-11T23:46:45Z (GMT). No. of bitstreams: 1 JoseRodolfoLopesDePaivaCavalcanti_TESE.pdf: 2156602 bytes, checksum: 000c3eafedc8a66a1a55425b0168fd4a (MD5) Previous issue date: 2015-04-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Sabe-se que o grupo das catecolaminas ? integrado pela dopamina, noradrenalina e adrenalina e que a s?ntese dessas subst?ncias se d? de modo sequencial, sendo a enzima tirosina-hidroxilase reguladora da fase inicial deste processo. Neste sentido, a 3- hidroxitiramina/dopamina ? precursora da s?ntese de noradrenalina e adrenalina e ainda possui a capacidade de atuar como neurotransmissor na por??o central do sistema nervoso. Os tr?s principais n?cleos dopamin?rgicos, chamados zona retrorubral (grupo A8), subst?ncia negra pars compacta (grupo A9) e ?rea tegmentar ventral (grupo A10), est?o dispostos na por??o die-mesencef?lica e est?o envolvidos em tr?s vias, a mesostriatal, mesol?mbica e mesocortical. Estas vias est?o relacionadas diretamente com diversas manifesta??es comportamentais como controle da motricidade, sinaliza??o de recompensa na aprendizagem comportamental, motiva??o e nas manifesta??es patol?gicas da Doen?a de Parkinson e esquizofrenia. Considerando-se a relev?ncia desses, o objetivo do trabalho foi caracterizar morfologicamente os n?cleos dopamin?rgicos (A8, A9 e A10) do sagui (Callithrix jacchus) mediante estudo citoarquitet?nico e imunoistoqu?mico contra tirosina-hidroxilase. O sag?i ? um primata neotropical, cujas caracter?sticas morfofuncionais repercutem na adequabilidade de uso deste animal em pesquisas de ordem biom?dica. Sec??es coronais dos enc?falos de seis animais foram submetidas ? colora??o pelo m?todo de Nissl e immunoistoqu?mica para tirosinsa-hidroxilase. Com base na morfologia dos neur?nios, foi poss?vel subdividir o grupo A10 em sete regi?es: n?cleo interfascicular, linear rostral e linear caudal, situados na linha m?dia; paranigral e o parainterfascicular, situados na zona intermedi?ria; a por??o rostral da ?rea tegmentar ventral e o n?cleo parabraquial pigmentado, situados na por??o dorsolateral do tegmento mesencef?lico. O grupo A9 foi subdividido em quatro regi?es: subst?ncia negra camadas dorsal e ventral; subst?ncia negra conjuntos lateral e medial. Por ?ltimo, n?o foram indentificadas subdivis?es no grupo A8. Conclu?-se que A8, A9 e A10 s?o filogeneticamente conservados entre as esp?cies, por?m percebe-se a necessidade de se ampliar os estudos acerca das organiza??es subnucleares, seja investigando a sua ocorr?ncia em outras esp?cies de primatas, seja investigando a sua relev?ncia funcional. / It is known that the catecholamine group is constituted by dopamine, noradrenaline and adrenaline, in which the synthesis is regulated by an enzyme named tyrosine hydroxylase. Thus, 3-hydroxytyramine/dopamine (DA) is a precursor of the noradrenaline and adrenaline synthesis and acts as a neurotransmitter in the central nervous system. The three main nuclei, named the retrorubral field (A8 group), the substantia nigra pars compacta (A9 group) and the ventral tegmental area (A10 group), are arranged in the die-mesencephalic portion and are involved in three complexes circuitries - the mesostriatal, mesolimbic and mesocortical pathways. These pathways are related to behavioral manifestations, motricity, learning, reward and pathologies such as Parkinson?s Disease and Schizophrenia. Thus, the aim of this study was to perform de morphological analysis of the A8, A9 and A10 nuclei of the common marmoset (Callithrix jacchus). The marmoset is a neotropical primate, whose morphological and functional characteristics supports the suitability of use of this animal in biomedical research. Coronal sections of the marmoset brain were submitted to cytoarchitectonic characterization and TH-immunohistochemistry. Based on the morphology of the neurons, it was possible to subdivide the A10 group in seven regions: interfascicular nucleus, raphe rostral linear nucleus and raphe caudal linear nucleus, in the middle line; paranigral and parainterfascicular nucleus, in the middle zone; rostral portion of the ventral tegmental area nucleus and parabrachial pigmented nucleus, located in the dorsolateral portion of the mesencephalic tegmentum. A9 group was divided into four regions: substantia nigra compacta dorsal and ventral tiers; substantia nigra compacta lateral and medial clusters. No subdivisions were founded into A8 group. These results revealed that A8, A9 and A10 are phylogenetically conserved between species, but it?s necessary to expand the studies about this compartmentalization, investigating its occurrence in other primate species or investigating its functional relevance.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-04-16T00:08:09Z No. of bitstreams: 1 HelderHenriqueAlvesDeMedeiros_DISSERT.pdf: 5892652 bytes, checksum: 483b84205306ccbbdee7acffe7f76a61 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-04-22T20:12:17Z (GMT) No. of bitstreams: 1 HelderHenriqueAlvesDeMedeiros_DISSERT.pdf: 5892652 bytes, checksum: 483b84205306ccbbdee7acffe7f76a61 (MD5) / Made available in DSpace on 2016-04-22T20:12:17Z (GMT). No. of bitstreams: 1 HelderHenriqueAlvesDeMedeiros_DISSERT.pdf: 5892652 bytes, checksum: 483b84205306ccbbdee7acffe7f76a61 (MD5) Previous issue date: 2015-06-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A 3-Hidroxitiramina/Dopamina (DA) ? uma monoamina do grupo dascatecolaminas e consiste na subst?ncia precursora da s?ntese de noradrenalina eadrenalina, tendo a enzima Tirosina-Hidroxilase (TH) como reguladora desse processo.Al?m disso, a DA tem a capacidade de atuar como neurotransmissor no SistemaNervoso Central - SNC, sendo o neurotransmissor principal de neur?nios de noven?cleos encef?licos, nomeados de A8 ao A16. Os n?cleos do mesenc?falo queexpressam DA s?o a Zona Retrorubral (RRF, grupo A8), a Subst?ncia Negra parscompacta (SNc, grupo A9) e a ?rea Tegmental Ventral (VTA, grupo A10). Tais n?cleosest?o envolvidos em tr?s complexas circuitarias que s?o a mesoestriatal, mesol?mbica emesocortical, os quais est?o relacionadas diretamente com diversas manifesta??escomportamentais como controle da motricidade, sinaliza??o de recompensa naaprendizagem comportamental, motiva??o e nas manifesta??es patol?gicas da Doen?ade Parkinson e esquizofrenia. Todavia, muitos aspectos de car?ter morfofuncionaldesses n?cleos ainda continuam sem esclarecimentos. Considerando a relev?ncia dosn?cleos dopamin?rgicos mesencef?licos, o objetivo deste trabalho ? caracterizarmorfologicamente os n?cleos dopamin?rgicos (grupamentos A8, A9 e A10) domesenc?falo do morcego (Artibeus planirostris). O Artibeus planirostris ? um morcegocomum no Rio Grande do Norte. Foram utilizados dez animais nesta pesquisa. Osanimais foram anestesiados, perfundidos e os enc?falos removidos da cavidadecraniana. Ap?s desidrata??o em sacarose, os enc?falos foram submetidos a microtomiae sec??es coronais foram obtidas e coletadas em seis compartimentos distintos. Oscompartimentos foram submetidos a colora??o pela t?cnica de Nissl para an?lisecitoarquitet?nica e as demais s?ries foram submetidas a imunoistoqu?mica para TH.Com base na t?cnica de Nissl e na imunoistoqu?mica para TH foi poss?vel verificar oslimites anat?micos, assim como a citoarquitetura e poss?veis subdivis?es dos tr?sn?cleos dopamin?rgicos do mesenc?falo. No sentido rostro-caudal, os primeirosneur?nios dopamin?rgicos a surgirem fazem parte da SNc e se estendem at? n?veis maiscaudais do mesenc?falo. A VTA surge nas sec??es rostrais e continua at? o n?vel caudal.No n?vel caudal surge a RRF. A SNc apresentou a exist?ncia de uma subdivis?o, acauda da subst?ncia negra, encontrada em apenas dois outros animais estudados. Opresente estudo indica que os n?cleos dopamin?rgicos do mesenc?falo do Artibeusplanirostris apresenta semelhan?as citoarquitet?nicas, bem como no padr?o dedistribui??o de neur?nios imunorreativos a Tirosina Hidroxilase em compara??o a10 outras esp?cies de mam?feros estudados, com pequenas varia??es, identificada nasubst?ncia negra. / The 3-Hidroxytyramine/dopamine (DA) is a monoamine of catecholamine family and isthe precursor substance synthesis of noradrenaline and adrenaline, having the enzymeTyrosine Hydroxylase (TH) as this regulatory process. In addition, the DA has theability to act as a neurotransmitter in the Central Nervous System - SNC, being themain neurotransmitter of brain nuclei, namely of A8 to A16. The nuclei of the midbrainthat express DA are the Retrorubral Field (RRF, A8), the Substantia Nigra parsCompacta (SNc, A9) and the Ventral Tegmental Area (VTA, A10). Such nuclei areinvolved in complex three circuitry that are the mesostriatal, mesolimbic andmesocotical and are directly related with several behavioral manifestations as motricitycontrol, reward signaling in behavioral learning, motivation and pathologicalconditions, such as Parkinson's Disease and schizophrenia. Interestingly, many of themorphological bases of these neural disturbance remain unknown. Considering therelevance of mesencephalic dopaminergic nuclei, the aim of this research is tocharacterize morphologically the dopaminergic nuclei (clusters A8, A9 and A10) of themidbrain of the bat (Artibeus planirostris). The Artibeus planirostris is a common bat inRio Grande do Norte. Ten animals were used in this research. The animals wereanesthetized, perfused, and the brain was removed from the skull. After dehydration insucrose, the brain was underwent microtomy. Saggital and coronal sections wereobtained and collected in six separate series. The series were Nissl-stained to identifythe cytoarchitectonic boundaries and the other series were subjected toimmunohistochemistry for TH. After cytoarchitectonic analysis and TH+ cellsidentification was possible to establish the anatomical boundaries of the nuclei, as wellas the subdivisions of three of the midbrain dopaminergic nuclei. The SNc is the mostrostral nucleus observed in the midbrain and is identified throughout the rostrocaudalextension of the midbrain. The VTA neurons were seen immediately caudal to the SNcappearance. The RRF neurons were observed just in the caudal levels of the midbrain.The SNc in Artibeus planirostris shows a particular feature, the tail of the SNc. The tailhave been described just in two other studied species. The present work shows aparticular variation in the organizational morphology of the SNc in the artibeus andcontribute to understand the phylogenetic routes by which the dopaminergic system hasevolved.