La presente investigación consta de un estudio de la novela El olvido que seremos, de Héctor Abad Faciolince, y busca trabajar el concepto de memoria que se desarrolla en dicho texto. En el primer capítulo, se pretende demostrar, a partir de una reconstrucción del personaje, por qué la pérdida del padre fue un acontecimiento central en la vida del narrador, quien, luego de un tiempo, buscará respuestas ante la amenaza que representa el olvido. En el segundo capítulo, partiendo de la idea de que, por lo que se desprende de la novela, el olvido es inminente, pero puede alargarse su llegada, se analizará cómo el autorpersonaje opta por seguir un camino: la escritura a nivel individual, de autor, la cual le permitirá alargar la llegada del olvido y pasar de la melancolía al duelo, siguiendo términos freudianos. Esta escritura también funcionará a nivel colectivo, en relación con el lector, como se verá en el tercer y último capítulo. En este, se planteará que la memoria individual es un primer paso para la construcción de una memoria colectiva, en la que el narrador encuentre en el lector a un receptor ideal con el que comparta experiencias vividas, empatice y, teniendo al padre como ejemplo de ser humano y héroe en la lucha por la defensa de los derechos humanos, forme una comunidad ética humanista liberal.

Amyloid beta (Aβ) has been postulated to be the principle initiator of the pathogenesis of Alzheimer’s disease (AD). Therefore, understanding the underlying mechanisms of Aβ induced neurotoxicity in the early stages of AD would be essential for finding potential therapeutic targets of AD. Aβ-binding alcohol dehydrogenase (ABAD) has been shown to be a mitochondrial binding site for Aβ. Expression of ABAD has been found to be increased in brains of AD sufferers. Two dimensional electrophoresis studies have revealed that endophilin 1 was upregulated in Tg mAPP/ABAD mice brains as compared to Tg mAPP, Tg ABAD and non-Tg mice brains. Increased expression of endophilin 1 has also been found in brains of AD patients as compared to non-demented control brain tissues. Endophilin1 has been reported to regulate c-Jun N-terminal kinase (JNK) activation. In this study, expression of dominant negative forms of endophilin 1 (DN-endophilin 1) in mouse cortical neurons exhibited a significant reduction of Aβ induced JNK activation. Furthermore, using cell counting methods, it was shown that the transfection of DN-endophilin 1 increased neuron survival after Aβ treatment. Aβ has also been proposed to disrupt the interaction of ABAD and Cyclophilin D (CypD), which would trigger mitochondrial permeable transition, thereby leading to neurotoxicity. For fluorescence resonance energy transfer (FRET) analysis of the interaction of ABAD and CypD, a mitochondria targeted, EYFP tagged ABAD plasmid (pMito-ABAD-EYFP) and an ECFP tagged CypD (pCypD-ECFP) plasmid were developed. Positive FRET signals in SK-N-SH cells co-expressing pMito-ABAD-EYFP and pCypD-ECFP indicated that ABAD interacts with CypD in the mitochondria of mammalian cells. RAGE (receptor for advanced glycation end products) has been reported to bind to Aβ and mediate the toxic effects of Aβ peptides on neurons and microglia. It has been shown previously that Tg mAPP/DN-RAGE mice display preserved cognitive function as compared to Tg mAPP mice. To investigate possible mechanisms involved in rescuing cognitive function by RAGE blockage, two dimensional electrophoresis was used to analyze differential protein expression between Tg mAPP and Tg mAPP/DN-RAGE mice cortex. Altered expression of four proteins, including NADH dehydrogenase flavoprotein 2 (NDUFV2), glyoxalase 1 (GLO1), proteasome subunit beta type 4 (PSMB4, or β7 subunit of proteasome) and nitrilase family, member 2 (Nit2) have been observed between Tg mAPP/DN-RAGE mice cortex and Tg mAPP mice cortex. NDUFV2 is a 24kDa subunit of complex 1 which is involved in ATP synthesis. GLO1 is a cytosolic enzyme that plays a role the glutathione-dependent detoxification of α-oxoaldehydes, such as methylglyoxal. PSMB4 is a subunit of the 26s proteosome which is in the degradation of ubiquitinylated proteins. The function of Nit2 is still unclear.

Submitted by Estagiário SPT BMHS (spt@fgv.br) on 2011-10-21T17:28:34Z No. of bitstreams: 1 000062416.pdf: 3818887 bytes, checksum: ae390965391235c0947fcd4a6d4c59d2 (MD5) / Approved for entry into archive by Estagiário SPT BMHS (spt@fgv.br) on 2011-10-21T17:28:45Z (GMT) No. of bitstreams: 1 000062416.pdf: 3818887 bytes, checksum: ae390965391235c0947fcd4a6d4c59d2 (MD5) / Approved for entry into archive by Estagiário SPT BMHS (spt@fgv.br) on 2011-10-21T17:28:53Z (GMT) No. of bitstreams: 1 000062416.pdf: 3818887 bytes, checksum: ae390965391235c0947fcd4a6d4c59d2 (MD5) / Made available in DSpace on 2011-10-21T17:29:00Z (GMT). No. of bitstreams: 1 000062416.pdf: 3818887 bytes, checksum: ae390965391235c0947fcd4a6d4c59d2 (MD5) Previous issue date: 1993 / EI presente trabajo se encamina a investigar el proceso evolutivo de las actuales Universidades tanto Hispanas como Portuguesas y llegar a otro proceso histórico en sus respectivas colonias en América, hasta llegar a las que son actualmente. Esto lleva necesariamente a un análisis - histórico - crítico dei desarrollo de estas instituciones cuyo desempefio sui géneris es intervenida por una serie de medidas administrativas cuyo origen es, en unos casos, de earácter cstrictamente administrativo. y cn otros, teniendo como [uenLe los criterios político-partidarios. Es asÍ que se pretende con el trabajo buscar qué o cuáles son las decisiones con ingerencia político-partidarias y cuáles las que son netamente administrativas, además, de que si estas decisiones son individuales o colectivas. Por tanto, se ha querido realizar una comparación cn lo que se refiere a Proceso Decisoriu entre la Universidad Federal de Rio de Janeiro-Brasil y la Universidad Nacional San Antonio AbadPerú, indudablemente una de las más importantes en cada uno de estas países. Finalmente. luego deI análisis critico, se presenta un aporte eon la inclusi6n de un esquema de Política Universitaria.

La presente investigación tiene como finalidad estudiar la novela colombiana El olvido que seremos (2006) de Héctor Abad Faciolince y el documental Carta a una sombra (2015) dirigido por Daniela Abad Lombana y Miguel Salazar Aparicio, desde la perspectiva de la memoria y de los modelos de paternidad que ambos relatos proponen. Tanto el libro como el documental tratan el asesinato de Héctor Abad Gómez, padre del escritor y abuelo de la cineasta a manos de sicarios desconocidos en Medellín. A partir de las nociones de “memoria ejemplar” (Todorov) y “posmemoria” (Hirsh), analizo la manera como los autores representan al padre asesinado, en el contexto de la violencia política que vivió Colombia en los años 80 y 90, así como los modelos de paternidad en una sociedad patriarcal.

The aim of this diploma thesis is to point out how personal experience and memories on the drug violence that was common in Colombia during 80's - 90's reflect in the novelistic works of a few contemporary Colombian writers. The thesis is based on the following five novels: Noticia de un secuestro (Gabriel García Márquez), El olvido que seremos (Hector Abad Faciolince), El ruido de las cosas al caer, Reputaciones (Juan Gabriel Vásquez) a La luz difícil (Tomás González). Aforementioned books were published after the year 2000 (Noticia de un secuestro makes an exception), thus they come from the writers that represent same generation. Almost all of the analyzed books are connected with the topic of terrorism of drug mafia and they take interest in the life of ordinary people. By analyzing novels, the thesis tries to point out also another topics, such as death or power of the fame and public opinion in the country. In each novel are analyzed also autobiographical features, language style and the process of the creation of the text. The last chapter contains memories and opinions of ten Colombian respondents which we can compare with the memories of the writers. Thanks to these opinions we can imagine not only the past of Colombia but partly also her current state. Key words: contemporary Colombian...

Una de las funciones de la universidad es realizar investigación orientada a resolver las demandas del sector productivo y social, es así que el presente proyecto de innovación se enmarca en el problema público “bajo nivel de vinculación de las actividades de investigación de la Universidad Nacional de San Antonio Abad del Cusco con las demandas del sector Agroindustrial y Turismo en la Región Cusco durante el periodo 2018 al 2020”. Este problema tiene como origen distintas causas, las mismas que fueron estudiadas a partir de información recogida mediante entrevistas y la recolección de datos. Entre las causas halladas están: 1). Burocracia en procedimientos administrativos de la UNSAAC relacionados a actividades de investigación; 2). Deficiente infraestructura de la UNSAAC para actividades de investigación; 3). Falta de incentivos por parte de la UNSAAC hacia sus investigadores para realizar trabajos colaborativos; 4). Falta de un canal de comunicación directo entre los investigadores de la UNSAAC y los sectores productivos; 5). Investigación más orientada a las ciencias básicas y no a la aplicada; y 6). La débil cultura investigativa en la UNSAAC. Como consecuencia de la identificación de causas, se eligió de acuerdo a determinados criterios la causa número 4 para reformular el problema público y plantear el siguiente desafío de innovación: ¿Cómo podemos modelar un canal de comunicación directo para los investigadores de la UNSAAC a fin de elevar el nivel de vinculación de las actividades de investigación con el sector Agroindustrial y Turismo de la Región Cusco? Como respuesta a esta interrogante se propone el Modelo Organizacional para la Colaboración entre la UNSAAC y el sector Agroindustrial y Turismo (MOC). Esta solución innovadora busca extender y fortalecer lazos de relacionamiento y colaboración, que a su vez sirva de canal de comunicación entre los investigadores de la UNSAAC y el sector Agroindustrial y Turismo de la región. Por otro lado, el MOC está compuesto por seis piezas de innovación, entre las que destacan los servicios que ofrece: investigación y desarrollo de proyectos, consultorías y asistencia técnica, y capacitaciones. Finalmente, el MOC cuenta con todas las condiciones para ser implementado y con ello elevar el nivel de vinculación UE y generar valor público. / One of the functions of the university is to conduct research aimed at solving the demands of the productive and social sector, thus this innovation project is framed in the public problem "low level of linkage of the research activities of the National University of San Antonio Abad del Cusco with the demands of the agro-industrial sector and tourism in the Cusco region during the period 2018 to 2020". This problem has different causes, which were studied on the basis of information gathered through interviews and data collection. Among the causes found are: 1). Bureaucracy in UNSAAC administrative procedures related to research activities; 2). Deficient UNSAAC infrastructure for research activities; 3). Lack of incentives from UNSAAC to its researchers to carry out collaborative work; 4). Lack of a direct communication channel between UNSAAC researchers and the productive sectors; 5). Research more oriented towards basic sciences and not applied research; and 6). Weak research culture at UNSAAC. As a result of the identification of causes, cause number 4 was chosen according to certain criteria to reformulate the public problem and pose the following innovation challenge: How can we model a direct communication channel for UNSAAC researchers in order to raise the level of linkage of research activities with the agro-industrial and tourism sector in the Cusco region? The Organisational Model for Collaboration between UNSAAC and the Agroindustrial and Tourism sector (MOC) is proposed as an answer to this question. This innovative solution seeks to extend and strengthen ties of relationship and collaboration, which in turn serves as a channel of communication between UNSAAC researchers and the agro-industrial and tourism sector in the region. On the other hand, the MOC is composed of six pieces of innovation, among which are the services offered: research and project development, consultancy and technical assistance and training. Finally, the MOC has all the conditions to be implemented and thus raise the level of EU linkages and generate public value.

Alzheimer's disease (AD) accounts for around two thirds of all dementia cases and an increase in life expectancy of the population has resulted in a substantial increase in dementia cases and with that a rise in AD. AD is a debilitating and ultimately fatal neurodegenerative disorder of the elderly, and despite being identified over a century ago, the current treatments do not treat the underlying causes behind the disease, instead they help to mask the symptoms of the disease and prolong the brain's remaining function. It is therefore vital that an effective, disease modifying treatment for this disease is established as soon as possible. Soluble intracellular forms of amyloid β (peptide Aβ), a hallmark of AD have been identified and intracellular targets of Aβ are being investigated as potential drug targets for the disease. Two key intracellular, mitochondrial proteins investigated as potential drug targets: amyloid binding alcohol dehydrogenase (ABAD) and cyclophilin D (CypD) are the focus of the work reported in this thesis. To begin identifying potential inhibitors of the ABAD-Aβ interaction, a two-pronged approach was taken. Firstly, a series of analogues based on a known inhibitor of the interaction were tested using a variety of biophysical assays, for their therapeutic affect on the interaction, and secondly a fragment based screening approach was used to identify new small molecule binding partners of ABAD which could potentially be modified to produced inhibitors of the ABAD-Aβ interaction. Three different CypD constructs have been successfully expressed and purified, and taken into crystal trials. It is hoped that these constructs can be used to significantly aid the progress of identifying any potential inhibitors and binding partners of CypD that may produce therapeutic effects, and in the future could lead to the identification of an effective disease modifying drug in the treatment of AD. The work reported in this thesis has built upon previously reported findings and the groundwork has also been established for several in vitro biophysical assays, these include for example: measuring ABAD enzyme activity, and the novel morphology specific Aβ aggregation assay, which can be used as screening tools to help identify potential inhibitors of these interactions. Both the ABAD-Aβ interaction, and the blockade of CypD are known to be drug targets in the treatment of AD, and by elucidating the molecular mechanisms behind these interactions, through implementing biophysical assays, this will help in the identification and design of potential new therapeutic agents for the treatment of AD.