This thesis applies catFISH, a variant of the standard fluorescence in situ hybridisation technique, to study the neuronal ensembles activated by heroin and cocaine across brain structures involved in motivated behaviour, in the Sprague-Dawley rat. The first chapter reviews the pharmacology of heroin and cocaine, rodent models of drug-related behaviours, and heroin and cocaine's ability to trigger immediate-early gene expression when administered acutely or chronically. It is suggested that the empirical evidence points towards a significant separation between the neuronal systems engaged by the two drugs. The main goal of this thesis was to test whether this separation can be seen within brain areas playing a key role in motivation and reward (e.g. the nucleus accumbens). Since immediate-early genes serve as markers of neuronal activity, and catFISH is a technique which can detect the expression of such genes in response to two separate stimuli, the technique was chosen as the best method to test if heroin and cocaine activate the same neuronal ensembles when administered acutely. The second chapter summarises the methods used across experiments described in following chapters. The third chapter presents an experiment addressing the methodological issues associated with using catFISH to study pharmacological stimuli. The technique was originally used to study the hippocampus and brain activity triggered by stimuli with well-controlled on- and offset (e.g. exposure to a novel environment or discrete cues). Arguably, acute drug injections comprise a stimulus with an on- and offset which can only be controlled indirectly – they depend on the drug dose and route of administration, among other factors. It was shown that acute intravenous injections of heroin and cocaine at doses usually self-administered by animals are suitable stimuli to use with catFISH. Chapter four describes an experiment showing that, across the striatum, the neuronal ensembles activated by an injection of cocaine followed by an injection of heroin overlap significantly less than the neuronal ensembles activated by two consecutive injections of cocaine. This is interpreted as direct evidence for a significant separation between the neuronal pathways activated by heroin vs. cocaine, even in brain areas often considered a common neurobiological substrate for the effects of the two drugs. It must be noted that the magnitude and the nature of this separation depends on the particular part of the striatum and the order in which drugs are administered. Chapter five describes a pilot experiment attempting to incorporate the logic of the catFISH technique into a rodent drug self-administration paradigm. It was found that the rats preferred self-administering heroin over cocaine, and that there was no detectable expression of the homer 1a gene across the striatum in response to acute heroin and cocaine after extended experience with the two drugs. There was also no change from baseline expression of the homer 1a and arc genes in areas of the prefrontal cortex. Finally, chapter six summarises the main findings and the key methodological considerations from all three experiments. As a whole, it is suggested that the experiments in this thesis provide a proof of concept that heroin and cocaine are processed differently by the brain, even within brain areas considered to be common substrates for the reinforcing and addictive properties of the two drugs. Future studies should take this separation into account, as it may have important implications for understanding drug addiction as a whole. The appendices contain representative fluorescence microscopy images of brain tissue analysed for catFISH.

Thesis (M.S.)--Central Connecticut State University, 2001. / Thesis advisor: Patti Lynn O'Brien. " ... in partial fulfillment of the requirements for the degree of Master of Science in Reading and Language Arts." Includes bibliographical references (leaves 42-44).

Thesis (M.A.) -- Central Connecticut State University, 2001. / Thesis advisor: Laura Levine. "...in partial fulfillment of the requirements for the degree of Master of Arts in General Psychology." Includes bibliographical references (leaves [26]-[31]).

Thesis (Ph. D.)--University of Texas at Austin, 2000. / Vita. Includes bibliographical references (leaves 150-174). Available also in a digital version from Dissertation Abstracts.

Thesis (M.A.)-University of KwaZulu-Natal, Durban, 2008. / Full text also available online. Scroll down for electronic link.

Thesis (MPH)--University of Queensland, 2003. / Includes bibliographical references.

Thesis (M. Soc. Sc.)--University of Hong Kong, 1983.

This study investigated how social understandings influence, and often obscure, individual perception. As a replication ofLindblom's (2002) study conducted in Sweden, the aim of this project was to investigate how individuals, studying towards a health care degree such as psychology, handle the occurrence of Child Sexual Abuse (CSA) when presented with it. To this end, participants were presented with a picture designed to represent CSA and were asked to interpret the nature of the picture. Following this, participants completed the Child Sexual Abuse Myth Scale (CSAMS) which served to highlight whether the three predominant social stereotypes surrounding CSA, namely, Blame Diffusion, Denial of Abusiveness and Restrictive Stereotypes, were influential in the interpretations participants offered surrounding the picture representing CSA. It was discovered that participants who scored significantly higher on the myth scale for the myths, Denial of Abusiveness and Restrictive Stereotypes, were more likely to misinterpret CSA when presented with it. / Thesis (M.A.)-University of Natal, Durban, 2004

This thesis investigates the phenomenological task of asking what the lived experience of the wounded healer is like for ex-drink/drug-addict Counselling Psychologists working in addiction. The wounded healer is a term that has been circulating in medical and psychotherapy circles for the past 150 years and has been associated with the helping professions and in particular addiction.

This study examined the interrelations among sexual victimization, attachment style, interpersonal relationship satisfaction, and substance use. Sexual victimization (i.e., child sexual abuse and adult sexual assault; CSA and ASA) is a major social concern for which further research is needed. While it is difficult to determine which difficulties are direct outcomes of sexual victimization, both CSA and ASA have been found to be associated with a variety of mental health problems, along with numerous other adverse outcomes across the lifespan (e.g., depression, risk of suicide, attachment insecurity, interpersonal relationship problems, substance abuse). By further exploring these relations and identifying potential mediating variables, specific therapy techniques may be tailored in order to address these variables in treatment. Factors such as attachment and interpersonal relationships are particularly important to consider when examining sexual victimization, due to the intimate nature of this type of victimization. It was expected that sexual victimization in women (controlling for other forms of childhood maltreatment) would predict insecure adult attachment, greater levels of harmful substance use (i.e., drug and alcohol abuse), and lower reported relationship satisfaction. Results indicated that women who experienced more severe CSA and anxious attachment engaged in greater levels of drug abuse (i.e., anxious attachment moderated the relation between CSA and drug abuse). In addition, women who experienced more severe ASA and child psychological abuse reported greater attachment insecurity (both attachment avoidance and anxiety) in their relationships and engaged in greater levels of substance abuse (i.e., both drug and alcohol abuse). Furthermore, women with greater levels of anxious attachment reported lower levels of relationship satisfaction. These findings suggest that clinicians working with women survivors of sexual victimization should be aware of potential attachment-related difficulties, as well as an increased risk of developing substance use problems that may be stemming from victimization experiences. / Graduate / 0621