Thesis (M.S.)--University of Wisconsin--Madison, 1970. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: 75-78.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1968. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography.

Thesis (Ph.D.)--University of Wollongong, 2008. / Typescript. Includes bibliographical references.

Made available in DSpace on 2014-07-29T15:30:35Z (GMT). No. of bitstreams: 1 Josireny-2006.pdf: 855560 bytes, checksum: 91e243f6dac631b98553944b5f290903 (MD5) Previous issue date: 2006-03-31 / Two million new cases of Leishmaniose occur annually in the tropical and subtropical areas of the globe, with a estimate of twelve million people currently infected in world and three hundred and fifty million under infection risk. The Leishmania sort, responsible for the disease, understands many diverse and complex species that present resistance to the drugs used in its treatment, beyond the characteristics undesirable. The effectiveness of the control and treatment of a parasitic disease depends on the detailed knowledge of the cycle of life, metabolism and biology in general of the parasite. The World Health Organization, associated with other agencies of research has stimulated the extract inquiry new of native plants as alternative for the treatment and combat of the parasitic disease In this work one standardized techniques of studies biochemists through liquid chromatography high performace (HPLC) evaluating organic acid excretion and consumption of in promastigotes of Leishmania amazonensis and became fullfilled biotests with Magonia pubescens (tingui) and Glycyrrhisa glabra (alcaçuz) in the concentrations of 25 50 and 100 mg.L-1 The acid had been detected following: lactate oxalate citrate -Ketoglutarate succinate fumarate malate and propionate demonstrated activities in the glycolytic pathway cycle of Krebs and respiratory chain Both the tested extracts had presented leishmanicid action with better effect for Glycyrrisa glabra 100 mg.L-1 and Magonia pubescens 25 mg.L-1 The Glycyrrisa glabra had better action in the stationary phase of growth to opposes it of Magonia pubescens that better acted in the logaritmic phase Chemical components of plants can act directly or indirectly in the metabolism of the parasite affecting essential metabolic pathways / Dois milhões de novos casos de Leishmaniose ocorrem anualmente nas áreas tropicais e subtropicais do globo com uma estimativa de doze milhões de pessoas atualmente infectadas no mundo e trezentos e cinqüenta milhões sob risco de infecção O gênero Leishmania responsável pela doença compreende muitas espécies diversas e complexas que apresentam resistência aos fármacos utilizados no seu tratamento além das características indesejáveis A eficácia do controle e tratamento de uma doença parasitária depende do conhecimento detalhado do ciclo de vida metabolismo e biologia em geral do parasito A Organização Mundial de Saúde associada a outros órgãos de pesquisas têm estimulado a investigação de novos extratos de plantas nativas como alternativa para o tratamento e combate das doenças parasitárias Neste trabalho padronizou-se técnicas de estudos bioquímicos através de cromatografia líquida de alta eficiência (CLAE) avaliando excreção e consumo dos ácidos orgânicos em promastigotas de Leishmania amazonensis e realizou-se bioensaios com Magonia pubescens (tingui) e Glycyrrhisa glabra (alcaçuz) nas concentrações de 25, 50 e 100 mg.L-1 Foram detectados os seguintes ácidos: lactato oxalato citrato -cetoglutarato succinato fumarato malato e propionato demonstrado atividades na via glicolítica ciclo de Krebs e cadeia respiratória Ambos os extratos testados apresentaram ação leishmanicida com melhor efeito para Glycyrrisa glabra 100 mg.L-1 e Magonia pubescens 25 mg.L-1 A Glycyrrisa glabra teve melhor ação na fase estacionária de crescimento ao contrario da Magonia pubescens que melhor agiu na fase logarítmica Constituintes químicos das plantas podem atuar diretamente ou indiretamente no metabolismo do parasito afetando vias metabólicas essenciais

In this study, firstly the effects of aspartic acid group amino acids -which were reported to be the potential bottleneck in serine alkaline protease (SAP) synthesis- on SAP production were investigated by substituting at a concentration range of 0-15 mM by using recombinant Bacillus subtilis carrying pHV1434::subC gene. All aspartic acid group amino acids except threonine inhibited SAP activity when CAA&amp / #8805 / 2.5 mM. The highest SAP activities with asparagine, aspartic acid, lysine, threonine, isoleucine and methionine werefound to be 1.89-, 1.87-, 1.61-, 1.48-, 1.4-, and 1.4-fold higher than the reference medium activity, respectively, when the concentration of each amino acid was CAA=0.25 mM. The product and by-product distributions in defined and complex media in SAP production were also analyzed and compared in order to obtain a depth in-sight on functioning of the metabolic reaction network. The highest SAP activity in complex medium was found to be 3&ndash / fold higher than defined medium activity, while, specific SAP production rate was 1.2- fold higher. The highest cell concentration in complex medium (CX= 14.3 g/dm-3) was 8.1-fold higher than that obtained in defined medium (CX= 1.75 g/dm-3). In both media, oxaloacetic acid was observed extracellularly and intracellularly. In complex medium there was also succinic acid in the extracellular medium indicating that the operation of TCA cycle was insufficient. In both media serine, valine and glycine were observed neither in the extracellular nor in the intracellular media indicating that the synthesis of these amino acids can be a secondary rate limiting step. In defined medium asparagine was present neither in the cell nor in fermentation broth whereas, methionine was observed in the cell in high amounts, probably due to the lower flux values towards asparagine. Thus, in defined medium the synthesis of asparagine can also be a potential bottleneck in SAP production in defined medium.