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CHARACTERIZATION OF THE PHOSPHODIESTERASE SUBTYPES THAT REGULATE MOUSE ATRIAL MYOCYTE ELECTROPHYSIOLOGYAdamczyk, Andrew 26 July 2011 (has links)
Phosphodiesterases (PDEs) are the enzymes responsible for the hydrolysis of cyclic nucleotides including cAMP and cGMP. We recently discovered that natriuretic peptides elicit effects in the atrial myocardium via a PDE dependant pathway; however, the role(s) of specific PDE subtypes in atrial myocytes are not clear. Thus, I studied the effects of PDE selective blockers on mouse atrial action potentials (APs) and L-type Ca2+ currents (ICa,L). AP duration (APD) was significantly increased in the presence of IBMX (inhibits all PDEs) as well as EHNA (PDE2 inhibitor) and rolipram (PDE4 inhibitor). The PDE 3 inhibitor milrinone had no effect on APD. Applying milrinone and rolipram (PDE3/PDE4 inhibition) or EHNA, milrinone, and rolipram (PDE2/ PDE3/PDE4 inhibition) in combination prolonged APD as effectively as IBMX. A similar pattern of results was obtained for atrial ICa,L. These data provide novel insight into the unique effects of PDE inhibitors in atrial myocytes
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Action shift : cyclically reflexive constructivist grounded action research informs pragmatic collaborative natural resource management strategies and tools for consideration by Fisheries and Oceans Canada, pacific regionFreethy, Diana 26 April 2012 (has links)
Grounded theory methodology blended with action research can provide creative
approaches to addressing policy-oriented questions with practical outcomes. Practical policy-oriented
research outcomes are illustrated through an integrated constructivist grounded action
research policy case study applied to collaborative natural resource management for Fisheries
and Oceans Canada's (DFO) consideration. The study developed grounded theory, which
reflexively informed collaborative strategies and supported action-oriented collaborative tool
development. Outcomes were developed to address each of three research questions through
cyclical reflexivity of researcher action shifts. Each action shift entailed cyclical reflexivity
through re-visitation of data in light of both developed grounded theory and previous research
question outcomes. As such, each question was addressed in reflexive cycles that built upon
previous research outcomes, which was complimented by authorial reflexively. This
constructivist grounded action bricolage demonstrates a reflexive, pragmatic, systematic
approach to policy-oriented recommendations and tool development. Reflexive constructivist
grounded action shift research supported strategic, integrated policy-oriented research outcomes
for DFO Pacific Region's consideration. The hope of this research is to encourage further
exploration of constructivist grounded action research as a dynamic, reflexive avenue that can
support integrated adaptive organizational policies and management.
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A process for evaluation and resource allocation in domestic public spending programsCase, Melvin Elwood 12 1900 (has links)
No description available.
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Regulation of CFTR Endocytosis by the Vasoactive Intestinal Peptide: Role of PKCεAlshafie, Walaa 02 December 2013 (has links)
The Vasoactive Intestinal Peptide (VIP) is an agonist of the CFTR chloride channel in the human airways. In the genetic disease Cystic Fibrosis, where CFTR is defective or absent from the apical membrane of epithelial cells, VIP innervations are lost. Our group has demonstrated that VIP increases CFTR membrane stability through PKCε. However, the mechanism remained to be determined. Here we found that VIP stimulation increases the interaction of NHERF1 and P-ERMs with CFTR through PKCε phosphorylation. Moreover a reduction of the interaction between intracellular CFTR and the Golgi associated protein, CAL was observed following VIP stimulation. Silencing either ERMs or NHERF1 with siRNA prevented the VIP ability to increase CFTR surface expression and function, confirming that NHERF1 and P-ERMs are necessary for VIP regulation of the sustained activity of membrane CFTR. This study shows the cellular mechanism by which prolonged VIP stimulation of airway epithelial cells regulates CFTR-dependent secretions.
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Trust-Building in the Construction Project Delivery Process: A Relational Lookahead Tool for Managing TrustSmith, James Packer 16 December 2013 (has links)
Low levels of productivity and recent evolutions in technology and practices are pushing the construction industry to collaborate on a higher level. A key component of effective collaboration is trust. Research also suggests that increased trust levels can lead to improved productivity in team performance. Trust appears to be valued by industry practitioners at the executive level but it also appears that active management of trust is minimal.
With Design Science Research methodology as a framework, this project uses a mixed methods approach to develop and test a tool designed to assist in the management of trust levels between construction project participants. This project lays the groundwork for additional research into trust-building in construction by testing whether or not trust can be actively built and managed by rigorous analysis of current and upcoming relationships. In addition to supporting data from case studies, this was accomplished by introducing specific trust-building techniques into student group interactions and comparing changes in interpersonal trust levels to a control group of students.
Results from the case studies and student experiment show some support for the idea that interpersonal trust levels, as perceived by the person making the attempts to build trust within the group, can be increased through use of a tool such as the one developed. Further testing and development is needed prior to wider industry application.
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Head Roll Influences on Multi-Sensory Integration for Perception and ActionBURNS, Jessica Katherine 21 July 2011 (has links)
The representation of ourselves and our environment is based on the combination of information from multiple sensory systems. Each sensory modality is represented within a different frame of reference, in other words each sensory system uses a different code to represent the same properties of the environment (ex. visual stimuli in an eye-centered frame of reference; hand position would be shoulder-centered). Combing this information into a singular coordinate frame is complex. For instance, the eye and shoulder have different centers of rotation, therefore any changes in eye position or body posture will affect the relationship between them. What is still unknown is how the brain integrates these different sources of information into an internal representation, and what effect extra-retinal signals can have on this process. This thesis was designed to investigate the effect of head roll on action and perception. In Experiment 1, we wanted to determine how the integration of vision and proprioception for action was affected by changes in head roll. To investigate this question subjects performed a reaching task at three different head roll positions, where they would experience conflicts between their viewed and actual hand position. In Experiment 2, we examined the influence of head roll on sensory perception. To explore this idea subjects performed a task where they needed to judge the position of their unseen index fingertip relative to a visual target. Our findings reveal that eccentric head roll conditions influence both action and perception – revealed by an increase in movement variability and a decreased ability to discriminate the position of the unseen fingertip relative to visual targets. In summary, we have discovered that introducing eccentric head roll positions affects the perception of ourselves within the environment and the way that we integrate sensory information. / Thesis (Master, Neuroscience Studies) -- Queen's University, 2010-09-21 14:07:09.217
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Widows of KilimanjaroGeekie, Constance Unknown Date
No description available.
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The schoolhouse dance in the Alberta grade four music program: an action research projectStark, David Stanley Unknown Date
No description available.
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Förskola för hållbar utveckling : Förutsättningar för barns utveckling av handlingskompetens för hållbar utvecklingHedefalk, Maria January 2014 (has links)
The overall aim of the thesis is to contribute with knowledge about the conditions for preschool children’s meaning making with regard to sustainable development. With a focus on critical actions, the thesis explores how education is executed and how a critical action may be conducted in a preschool practice. Different teaching situations in preschool have been analysed in order to determine how and which actions are privileged in the various situations, what the consequences of these actions are, and what kind of conditions that enable children to develop critical action competence. The results show that teachers affect children’s meaning making by directing actions toward a specific learning content. The conditions in which children learn action competences for sustainable development may be affected by which actions are privileged or excluded in the situation. The analyses of conditions for children to act critically in this thesis are when: the teacher aims the attention towards a pluralistic teaching content, where the content consists of value judgments. variations of views are highlighted – by the help of other children or from example different viewpoints in a book. children have the courage or/and feel safe expressing contradicting views. Although the situations in which children act critically are few and far between in the empirical material, they do exist and are important to highlight, especially as few other studies explore what meaning making processes look like when critical actions are privileged. The thesis therefore contributes to the research by providing analyses of situations in which children act critically in preschool.
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Narrating the past to vision the future: constructing civil society with women in UkraineFlaherty, Maureen P. 07 April 2011 (has links)
Peace processes require an opening to self and others — a willingness to confront what is and to vision beyond present challenges to a brighter future. This type of engagement is crucial for the peaceful development of healthy, functioning societies — societies such as Ukraine, a country thrust without preparation from regional Soviet status to independent country searching for democracy. Eighteen years post-Independence the Ukrainian parliament continues to flounder unsupported by citizens. Active participation in civic affairs required for democracy is unfamiliar for most Ukrainian citizens, having internalized centuries of divisive oppression under a series of authoritarian regimes. Democracy-building and peace-building require participant agency and voice; rising out of oppression, people often need support to speak about and transform their lived experiences. This study, cognizant of the centrality of gender analysis in any context, explored the roles women’s shared narrative, dialogue, and group-visioning play in the support of personal empowerment and bridge building between diverse communities. The study invited women from the European Union-focused Western region of Lviv, Ukraine and the more Soviet/Russian-identified Eastern region of Crimea, first to share their personal stories with the researcher and second, to meet in their regional groups to vision for themselves, their families, and Ukraine. The third phase of this study invited these diverse regional groups to meet in a neutral space, reflexively exploring their parallel processes, while in phase four participants reviewed their experiences of the study. Despite initial beliefs that they have little in common, women in both regions said study participation changed them. They found telling their stories “from beginning to end” allowed them to reflect upon their own values and strengths, and having connected with themselves and their roots, they were then able to reach out to others. Rather than looking for differences, participants sought ways to express a shared vision for an inclusive, functional, peace-building future for themselves, their families, and Ukraine as a whole.
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