Cbf11 and Cbf12 proteins, the members of the CSL transcription factors family, are involved in a wide range of cellular processes in the fission yeast Schizosaccharomyces pombe - among other things they regulate cell adhesion and they have also been implicated in maintenance of genome integrity. At the level of the whole genome we previously identified target loci bound by CSL proteins in vivo. Many of them do not contain any consensus CSL-binding element. There are probably different DNA binding modes of the Cbf11/12 proteins and it has not been known what specific biological function is associated with the particular way of DNA binding. For the purpose of studying CSL DNA binding modes we have worked in this project on the implementation of the DNA binding mutation (DBM), which prevents direct DNA binding of CSL proteins to canonical motif in vitro, into the chromosomal locus of the cbf11 and cbf12 genes. Using the "ura4 selection system" we have successfully constructed the scar-less Cbf12-TAP and Cbf12DBM-TAP knock-ins, i.e. the strains without/with DBM in the open reading frame of Cbf12 where Cbf12 is C- terminally TAP-tagged and contains the intact 3'UTR. In our laboratory we have established the CRISPR/Cas9 system by which we have been able to prepare the Cbf11- TAP strain. We have failed to...

The presented dissertation thesis is focused on investigating the adhesive properties of façade cladding made of wood and wood-based materials in combination with flexible adhesion/assembly systems. The systems allow the implementation of effective construction joints and provide a solution with uniform stress distribution which allows the transfer of heavy loads, while simultaneously reducing the weight of the entire structure, which is also often connected with substantial cost reductions. Despite all that, little attention has been paid to this area so far. It is not a solution which is properly embodied in technical standards or in designers´, architects´ and contractors´ awarenesses. The aim of this dissertation is to characterise adhesive properties of selected façade cladding representatives made of wood and wood-based materials for the purposes of bonded joint application in a ventilated façade system. To define adhesion of façade joints, numerical and experimental methods are used, taking also various limit conditions into account. The main conclusion of the dissertation is the characterisation of basic parameters which influence adhesion, effectiveness and quality of façade glued joints. The secondary conclusion is the compilation of a simple methodical procedure for testing and implementing façade glued joints. The dissertation also makes a contribution by extending the current state of knowledge of façade adhesive joints.

The present thesis deals with optimization of the formation of thin layers of metallic materials. In the theoretical part was elaborated overview of the implementation of the formation of thin- film structures, particularly using vacuum techniques. Chemical and physical vapor deposition. They were described methods of pretreatment of thin layers. The findings were discussed methods of controlling the pre-treated surface, quality control and mutual adhesion of coating layers. In the experimental part of the pre-treatment methods were selected and applied to selected substrates. Pretreatments were evaluated using a control surface by wetting angles and by atomic force microscopy. Then, the metal structure formed of copper on the pretreated substrates. Implementation by means of magnetron sputtering. Adhesion layers was checked by testing the surface lattice method. The quality was observed by scanning electron microscopy. The results were used to design the technological process of making metal layers.

n this work is processed a summary of selected driving parameters of vehicles, methods of measuring and use in the automotive industry or related branch especially. This work is focused on driving tests of vehicles and measurement driving parameters of vehicles. As part of this work was done measurements parameters of vehicles driving in various driving regimes and subsequent analyse of obtained data. The result of this work is determination methodology division driving regimes and evaluation the impact of driving regimes on to ride a car in a directional curve.

Disertační práce se zabývala vlivem adheze mezi vláknovým (FRC) a částicovým (PFC) kompozitem a složením obou komponent na mechanické vlastnosti a způsob porušování modelových bi-materiálových kompozitních těles při statickém namáhání. Zkoumán byl také vliv způsobu přípravy bi-materiálového kompozitního tělesa na pevnost adheze mezi jeho kompozitními komponentami. K hodnocení mechanických vlastností bi-materiálových PFC/FRC těles byl použit jak 3 tak 4-bodový ohybový test za pokojové teploty a relativní vlhkosti 70%. Modifikovaný vytrhávací test byl použit k měření smykové pevnosti adheze mezi vláknovým a částicovým kompozitem. Tyto výsledky byly korelovány s výsledky ze strukturní a fraktografické analýzy (TGA, SEM). Experimentální data byla poté analyzována pomocí existujících mikromechanických modelů a byl nalezen vztah mezi tuhostí modelových bi-materiálových těles, složením a geometrií uspořádání jejich komponent a pevností adheze mezi těmito komponentami. Na základě těchto výsledků byl navržen optimální způsob vrstvení a přípravy PFC/FRC bimateriálových těles. Navržené postupy byly použity k přípravě a pre-klinickým testům nosných konstrukcí zubních můstků.

Disertační práce se zabývá studiem povrchových vlastností jedno a vícevrstvých filmů deponovaných z vinyltriethoxysilanových a tetravinylsilanových monomerů. Zabývá se také charakterizací adheze jednovrstvých filmů z tetravinylsilanu. Plazmaticky polymerizované tenké vrstvy byly připraveny na leštěných křemíkových substrátech pomocí plazmové depozice z plynné fáze za ustálených podmínek. Povrchové vlastnosti vrstev byly charakterizovány pomocí různých metod rastrovací sondové mikroskopie a nanoindentačních technik jako je konvenční a cyklická nanoindentace. Vrypový test byl použit pro charakterizaci vlastností adheze vrstev. Jednovrstvé filmy připravené za různých depozičních podmínek byly charakterizovány s ohledem na povrchové morfologie a mechanické vlastností (modul pružnosti, tvrdost). Výsledky morfologie povrchu, analýzy zrn, nanoindentace, analýzy konečných prvků a modulů mapování pomohly rozlišit hybridní charakter filmů, které byly deponovány při vyšších výkonech RF-výboje. Nový přístup byl použit v povrchové charakterizaci vícevrstvého filmu pomocí rastrovací sondové mikroskopie a nanoindentace. Adhezívní chování plazmaticky polymerizovaných vrstev různých mechanických vlastností a tloušťek bylo analyzováno pomocí normálních a laterálních síl, koeficientu tření, a snímků vrypů získaných pomocí mikroskopie atomárních sil.

Diploma thesis describes technology of pulse reactive magnetron sputtering of Al2O3 coating. Furthermore it deals with design of deposition process and testing new aluminium target. The technological window was determined by tests. As a result, it was possible to create Al2O3 coatings in the widest possible working range. The coatings created by apparatus PLS 160 were tested for optical properties in order to obtain transmittance. Besides the optical tests, the evaluation of mechanical properties and adhesion were performed. The obtained results from measurement were evaluated. Based on the results, some possible applications of the coating were suggested.

The thesis is based on articles describing the fundamental research of carbon based nanomaterials for their possible utilization in biomedicine. The aim of this thesis was to describe the way how human osteoblasts (SAOS-2 cell line) and primary human mesenchymal stem cells (hMSC) adhere, grow and behave on surfaces made of several carbon allotropes - nanocrystalline diamond (NCD), single walled carbon nanotubes (SWCNTs) films and graphene. The utilization of carbon as the basic material promised good biocompatibility and possibility of useful surface modifications. The NCD had modified surface nanotopography (nanoroughness and nanostructuring prepared by dry ion etching). All the materials had modified surface atomic termination with oxygen and hydrogen which changes the surface electrical conductivity, surface charge and wettability. It was hypothesized that the surface termination can also influence the cell adhesion and growth. It turned out that all the studied materials were suitable as substrates for cultivation of mentioned cell types. Various nanoroughnesses of NCD surface had different effect on the cell adhesion and cell metabolic activity. Nanostructuring of the NCD influenced the formation of focal adhesions. The surface terminations of NCD and the other studied nanomaterials in...

Diploma thesis deals with glass fiber surface modification using plasma-enhanced chemical vapor deposition in order to prepare functional interface that enhances the properties of polymer composites. The effect of deposition conditions on shear strength was observed with respect to the chemical composition of the deposited film. Thin films were deposited on planar substrates and fibers using monomer tetravinylsilane in a mixture with oxygen at selected power of plasma discharge. Chemical composition of prepared material was analyzed by infrared spectroscopy. Planar substrate film adhesion was measured using the scratch test. The composite sample was prepared by embedding the surface modified fibers in unsaturated polyester resin, followed by the curing process. The cured composite sample underwent the short beam shear test.

The work was comprehensively focused on the development of adhesive forms of probiotics in the form of a biofilm on combined carriers with a prebiotic component. The second part dealed with the influence of food on the multiplication and survival of selected types of probiotic bacteria. Subsequently, the effect of individualized probiotic supplements on changes in the human intestinal microbiome was monitored. Suitable adherent probiotic strains for biofilm formation were selected and tested. Methods have been introduced and different variants of carriers for culturing and binding bacteria have been tested. In vitro experiments verified the stability of biofilm stucture and its resistance to low pH, bile and antibiotics in comparison with the planktonic cell form. The antimicrobial effect of probiotic strains in the form of a biofilm was studied. The cultivation of the multispecies biofilm on the combined carrier was optimized and the stability of the biofilm and the final viability of probiotic bacteria were confirmed. Furthermore, the influence of various foods and beverages on the viability of probiotic bacteria was evaluated with emphasis on the simulation of passage through the gastrointestinal tract. Both models, solutions with standardised concentrations of alcohol, sugar, salts, proteins or different pH and different types of real foods and beverages were tested. The effect of food and beverages was tested on monocultures of Lactobacillus acidophilus, Bifidobacterium breve and on probiotic capsules containing a mixed culture of probiotic microorganisms. The survival of probiotics in various food matrices in the simulated gastrointestinal tract was quantitatively different. We managed to define foods suitable for supporting the multiplication of probiotic bacteria. A separate part of the work was focused on the targeted modulation of the intestinal microbiome by individualized probiotics that were prepared on the basis of molecular biological analyzes of the intestinal microbiome aimed at detecting the percentage of lactobacilli, bifidobacteria and phylum Firmicutes and Bacteroidetes. Personalized probiotic supplementation confirmed the positive effect of this approach on microbiome changes, especially on the increase of the content of lactobacilli, bifidobacteria and the overall diversity of the microbiome.