Introduction: There is progressive stiffening and dilatation of the aorta and large elastic arteries with aging as a result of the repetitive cyclic stress they are exposed to throughout life. Aortic stiffening has a number of detrimental effects including an increase in aortic pulse wave velocity and early wave reflection, isolated systolic hypertension, ventricular-vascular mismatch, impaired coronary blood flow reserve, and is the fundamental cause of heart failure in the elderly. The aim of this thesis is to provide proof of concept for the aortic wrap procedure; a surgical treatment of stiffening and dilatation of the ascending aorta with aging. The surgical procedure involves wrapping an elastic material around the ascending aorta of elderly patients, to reduce the stiffness and diameter of the ascending aorta towards that seen in youth. Methods: Proof of concept is investigated in the following studies. 1. The effect of the elastic wrap on the in-vivo stiffness of the normal aorta. 2. The effect of the elastic wrap on the in-vivo stiffness of the dilated and stiffened aorta. 3. The effect of the elastic wrap on the in-vitro stiffness of the aged human ascending aorta and on aortic pressure. 4. The effect of wrapping the aged human ascending aorta on pulse pressure (mathematical model). 5. The effect of chronic implantation on the structure of the normal aorta. 6. The mechanical properties of the ovine thoracic aorta and the elastic wrap material. Results: 1. Elastic wrap application increased the in-vivo stiffness of the normal aorta. 2. Elastic wrap application decreased the stiffness of the stiffened and dilated aorta. 3. Elastic wrap application decreased the in-vitro stiffness of the elderly human ascending aorta and pulse pressure. 4. A reduction in ascending aortic stiffness was sufficient to reduce ascending aortic pulse pressure. Conclusion: Application of the elastic wrap to the aged human ascending aorta is expected to reduce aortic stiffness, as well as systolic and pulse pressure, and to increase diastolic pressure with a reduction in cardiac load. The aortic wrap procedure may be an effective surgical procedure for the treatment of heart failure and isolated systolic hypertension.

This thesis details four consecutive research investigations which were designed to examine the effect of age on the pulmonary oxygen uptake (VO2)and muscle oxygenation (mOxy) responses to exercise in well-trained cyclists. (Abridged)

Rapid aging of the majority and minority populations is occurring in the United States. Diverse aspects of aging, concerns of the aging of the baby boomer generation, and the heterogeneities among aging persons from diverse races and ethnicities have been examined in detail; however, there is a void in the literature that offers information about the aging of Chinese baby boomers. This qualitative study used a hermeneutic-phenomenological method to investigate the aging experiences of Hong Kong Chinese baby boomers who are residing in the United States. The findings of this study will possibly enhance the literature about the cultural parameters that relate to these persons' aging. The information can benefit counselors whose work involves this population. The results of this study are consistent, highlighting Hong Kong baby boomers' sensitivities to health issues, their perceived physiological changes and change in personality attributes, their desires to strengthen relationships with families and friends and to practice preventive healthcare, their concerns about losing independence, and persons that are important to their aging journeys such as children, spouses, and friends. From an existential perspective based on lived experiences (specifically corporal, temporal, spatial, and relational), the participants' subjective somatic experiences reflected some negative changes such as decline in memory and a lower level of energy. Their temporal sensibilities motivated them to do things that are desirable and meaningful. Their subjective sense of space would be the settings where they nurture their relationships and maintain their active lifestyles. Their relationships seemed to be marked by their longing to see their children often and to grow old with their spouses and friends. The findings indicate that these participants are negotiating their aging identities, autonomy, generativity, and integrity. The factors that protect these baby boomers, such as positive attitudes and sound health, are consistent with the adaptive means that relate to their aging. The importance of this investigation culminates in the cultural meanings of these findings, as they can enrich the counselor education knowledge base. Counselors must develop awareness and understanding of these meanings and integrate them into their work with baby boomer clients of Chinese descent. / School of Education / Counselor Education and Supervision (ExCES) / PhD / Dissertation

Thesis (Ph. D.)--University of Texas at Austin, 2006. / Vita. Includes bibliographical references.

Aging is characterized by a progressive decline in cardiac function. A critical contributor to the age-related impairment in heart function is the loss of cardiac myocytes through ??apoptosis??, or programmed cell death. A dramatic increase in the rate of apoptosis has been reported with aging in the rat left ventricle. In contrast, exercise training not only improves cardiac function, but also reduces the risk of heart disease. However, the ability of exercise training to modulate apoptotic signaling and apoptosis in the aging heart remains unknown. Therefore, the purpose of this study was to determine the effects of exercise training on apoptotic signaling and apoptosis in the aging heart. We hypothesized that (1) aging would increase pro-apoptotic signaling and apoptosis in the rat left ventricle, and (2) exercise training would ameliorate upregulation of Bcl-2 family-driven apoptosis in the heart. Four and 25 month old Fischer-344 rats were assigned to four groups: young control (YC), young trained (YT), old control (OC), and old trained (OT). Exercise training groups ran on a treadmill for 60 min/day at 15 m/min (15˚ incline), 5 d/wk for 12 wk. Protein expression of Bax, Bcl-2, caspase-9, and cleaved caspase-3 was measured using Western immunoblot analysis. Apoptosis (DNA fragmentation) was assessed using a cell death detection ELISA. Bax levels in OC were dramatically higher (+176.0%) compared to YC. In contrast, exercise training resulted in a significant decrease (-53.4%) in Bax in OT compared to OC. Bcl-2 levels in OC were lower (-26.3%) compared to YC. Conversely, exercise training significantly increased Bcl-2 levels by 117.8% in OT compared to OC. Caspase-9 levels were higher (+98.7%) in OC than YC, while exercise training significantly reduced caspase-9 levels in YT (-52.6%) and OT (-76.9%), respectively. Aging resulted in a dramatic increase (+122.8%) in cleaved caspase-3 levels and a significant decrease (-32.9%) with exercise training. Finally, apoptosis (DNA fragmentation) significantly increased (+163.8%) with aging and decreased (-43.9%) with exercise training. These novel data indicate that aging increases pro-apoptotic signaling and apoptosis in the left ventricle, while exercise training is effective in diminishing pro-apoptotic signaling and apoptosis in the aging heart.

A large body of research has investigated various aspects of attention in normal aging and Alzheimers disease (AD). Most of the previous studies have shown that divided attention, the ability to attend to two tasks or stimuli simultaneously, declines in both normal aging and AD. In a recent study of attention, Baddeley, Baddeley, Bucks, and Wilcock (2001) reported findings that contrast with other divided attention research. Specifically, they found no effects of aging on divided attention. Taken in combination with their findings of age and AD effects on other aspects of attention, the authors concluded that age-equivalent results on divided attention tasks support the theory that attentional control should be viewed as a fractionated system. Study 1 considered methodological differences between the divided attention tasks used by Baddeley et al., and the tasks used by researchers who have reported age-related differences. Specifically, the effects of task difficulty on age effects were examined. Young, middle-aged, and older adults were compared on a dual-task procedure that combined a secondary visuomotor task (box joining) with a primary verbal task (month reciting) administered at two levels of difficulty. Results showed a significant Age x Task Difficulty interaction. That is, differences among age groups were proportionately greater in the difficult dual-task condition versus the easy condition, suggesting that age-related declines in divided attention may only be detected if tasks are sufficiently difficult.<p> Study 2 examined attention in normal aging and AD. Young adults, older adults, and early-stage AD patients were compared on tasks of selective attention, focal attention, and divided attention, with each task administered at two levels of difficulty. Similar Group x Task Difficulty interaction effects were detected for all attentional tasks, a finding which is more consistent with a general-purpose model than a fractionated model of attention. Study 3 considered attentional tasks from a clinical perspective. Specifically, the attentional tasks utilized in Study 2 were examined with respect to their ability to correctly classify individuals with early-stage AD and normal older adults. Findings showed that all attentional tasks successfully discriminated patients from cognitively healthy older adults, with one task of divided attention showing particularly impressive sensitivity and specificity. Findings of the three studies are discussed with regard to their implications for future research and clinical practice.

Over the past two decades, high-throughput (HTP) technologies such as microarrays and mass spectrometry have fundamentally changed the landscape of aging and disease biology. They have revealed novel molecular markers of aging, disease state, and drug response. Some have been translated into the clinic as tools for early disease diagnosis, prognosis, and individualized treatment and response monitoring. Despite these successes, many challenges remain: HTP platforms are often noisy and suffer from false positives and false negatives; optimal analysis and successful validation require complex workflows; and the underlying biology of aging and disease is heterogeneous and complex. Methods from integrative computational biology can help diminish these challenges by creating new analytical methods and software tools that leverage the large and diverse quantity of publicly available HTP data. In this thesis I report on four projects that develop and apply strategies from integrative computational biology to identify improved biomarkers and therapeutics for aging and disease. In Chapter 2, I proposed a new network analysis method to identify gene expression biomarkers of aging, and applied it to study the pathway-level effects of aging and infer the functions of poorly-characterized longevity genes. In Chapter 4, I adapted gene-level HTP chemogenomic data to study drug response at the systems level; I connected drugs to pathways, phenotypes and networks, and built the NetwoRx web portal to make these data publicly available. And in Chapters 3 and 5, I developed a novel meta-analysis pipeline to identify new drugs that mimic the beneficial gene expression changes seen with calorie restriction (Chapter 3), or that reverse the pathological gene changes associated with lung cancer (Chapter 5). The projects described in this thesis will help provide a systems-level understanding of the causes and consequences of aging and disease, as well as new tools for diagnosis (biomarkers) and treatment (therapeutics).

There is evidence to suggest that people from different cultures have different cognitive processing styles. For example, by measuring the eye movements of American and Chinese students when viewing pictures, Chua, Boland, and Nisbett (2005) found that American students fixated more on the focal object, whereas Chinese students fixated more on the background. In a subsequent object-recognition task, the Chinese students were less likely to correctly recognize old objects presented in new backgrounds than Americans did. This study used a similar scene-viewing task to investigate whether aging modulates these cultural differences in cognitive processing style. Like Chua et al., we found that young Chinese students spent longer fixating the background than did their Western counterparts. However, we failed to replicate the accompanying memory bias observed by Chua et al. Our strongest finding was that maintaining the original background facilitated memory for objects in young participants of both cultures but not for older participants. This result suggests that older adults had poorer memory for background details and/or had poorer integration of object and background.

While most seniors are capable, conscientious drivers, some have experienced age-related declines in the skills that support safe driving, thus pose a hazard to themselves and to other road users. There seems to be agreement that older adults should stop driving when their skills have declined to the point that they pose a risk to themselves and others, but there are few guidelines to aid older drivers or their families in determining when one should no longer drive. This study was designed to identify driving behaviors and non-driving measures that predict hazardous driving errors such as leaving the roadway or hitting pedestrians other cars. Ten younger and 30 older adults participated in the study; older participants were divided into three groups of 10 based on performance on cognitive screening tests. Participants completed tests of attention, working memory, spatial memory and timing ability as well as simulated driving scenarios. The younger participants made significantly fewer hazardous errors than did the older drivers with poorer cognitive performance. Driving behaviors associated with increased hazardous errors in older participants included poor maintenance of lane position in low complexity condition and making abrupt lane changes when complexity increased. Poor performance on a measure of working memory and on an anticipation timing task were also associated with increased hazardous errors in the older participants. / Thesis (Ph.D.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of Psychology / "December 2005."

Unlike in laboratory compacted asphalt mixtures, the distribution of viscoelastic properties in field layers is not uniform because of nonuniform air void distribution and aging. Therefore, characterization of field specimens is more challenging compared to that of laboratory compacted specimens. Formerly, characterization of field asphalt mixtures was based on binder tests which are useful but do not represent the properties of the asphalt mixtures because binder is only a component in the asphalt mixture. This study uses linear viscoelastic theory and numerical modeling to obtain the undamaged and damaged viscoelastic properties of both laboratory made and field compacted asphalt concrete. Additionally, it uses fracture mechanics principles to find the fracture and healing properties of aged asphalt specimens. The analytical models presented in this research have been successfully verified by testing the actual field specimens of different ages. The model developed in this dissertation is suitable to track the viscoelastic, fracture and healing properties of the field specimen with time and depth. The test protocols and analytical models described in this study can be used for the development of reliable performance models for field-aged asphalt layers.