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The effect of different ozone concentrations on white blood cell energy homeostasis / Lissinda H. du PlessisDu Plessis, Lissinda Hester January 2006 (has links)
Ozone therapy is an alternative form of therapy that has gained attention in the last
couple of years. It is believed that O3 may exert a stimulatory effect on the antioxidant
defence and immune systems and may therefore be effective in the treatment of ischemic
disorders. diabetes mellitus. AIDS and other diseases. On the other hand. it is well known
that O3 is a reactive molecule that is toxic to the pulmonary system. Therefore. there
remains scepticism regarding its use as a form of therapy. In order to shed some light on
this. the effects of ozone autohemotherapy (O3-AHT) on the energy homeostasis of white
blood cells were investigated. The possible protective effects of the plasma antioxidant
defence system during O3-AHT, were also investigated.
Venous blood from six apparently healthy human donors was collected in heparin. In
one aliquot a precise volume of blood was mixed with an equal volume of O2/O3 gas
mixture containing 20 or 80 μg/ml O3 for 20 minutes. In the other aliquot, the plasma
was washed out and the cells resuspended in a buffered phosphate solution. The buffered
blood cells were treated with the same concentrations of O3. Control samples was either
not treated or treated with a corresponding volume of O2 . Various biochemical analyses
were done on the whole blood and buffered cells to determine the oxidant/antioxidant
status, cell viability, apoptosis and mitochondrial function.
The higher concentration of O3 increased oxidative stress and caused death of white
blood cells. Antioxidant enzyme (catalase, glutathione reductase and glutathione
peroxidase) activity and the plasma antioxidant capacity decreased, whereas superoxide
dismutase levels increased slightly. Exposure to O3 also increased caspase 3/7 activity. A
decrease in mitochondrial function was measured by a decrease in ATP levels and an
increase in NADH/NAD+ ratio. Complex IV of the respiratory chain was almost
completely inhibited by both O3 concentrations. These results indicated that the death of
white blood cells was probably through apoptosis. These effects were more evident in the
absence of plasma antioxidants. Therefore. high concentrations of O3 were damaging to
the cells, but this effect was lessened by antioxidants present in plasma. In view of the
results, the use of O3 as a therapy needs to be reconsidered. / Thesis (Ph.D. (Biochemistry))--North-West University, Potchefstroom Campus, 2007.
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The effect of different ozone concentrations on white blood cell energy homeostasis / Lissinda H. du PlessisDu Plessis, Lissinda Hester January 2006 (has links)
Ozone therapy is an alternative form of therapy that has gained attention in the last
couple of years. It is believed that O3 may exert a stimulatory effect on the antioxidant
defence and immune systems and may therefore be effective in the treatment of ischemic
disorders. diabetes mellitus. AIDS and other diseases. On the other hand. it is well known
that O3 is a reactive molecule that is toxic to the pulmonary system. Therefore. there
remains scepticism regarding its use as a form of therapy. In order to shed some light on
this. the effects of ozone autohemotherapy (O3-AHT) on the energy homeostasis of white
blood cells were investigated. The possible protective effects of the plasma antioxidant
defence system during O3-AHT, were also investigated.
Venous blood from six apparently healthy human donors was collected in heparin. In
one aliquot a precise volume of blood was mixed with an equal volume of O2/O3 gas
mixture containing 20 or 80 μg/ml O3 for 20 minutes. In the other aliquot, the plasma
was washed out and the cells resuspended in a buffered phosphate solution. The buffered
blood cells were treated with the same concentrations of O3. Control samples was either
not treated or treated with a corresponding volume of O2 . Various biochemical analyses
were done on the whole blood and buffered cells to determine the oxidant/antioxidant
status, cell viability, apoptosis and mitochondrial function.
The higher concentration of O3 increased oxidative stress and caused death of white
blood cells. Antioxidant enzyme (catalase, glutathione reductase and glutathione
peroxidase) activity and the plasma antioxidant capacity decreased, whereas superoxide
dismutase levels increased slightly. Exposure to O3 also increased caspase 3/7 activity. A
decrease in mitochondrial function was measured by a decrease in ATP levels and an
increase in NADH/NAD+ ratio. Complex IV of the respiratory chain was almost
completely inhibited by both O3 concentrations. These results indicated that the death of
white blood cells was probably through apoptosis. These effects were more evident in the
absence of plasma antioxidants. Therefore. high concentrations of O3 were damaging to
the cells, but this effect was lessened by antioxidants present in plasma. In view of the
results, the use of O3 as a therapy needs to be reconsidered. / Thesis (Ph.D. (Biochemistry))--North-West University, Potchefstroom Campus, 2007.
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In sii atla nis kwii sii yuk mit kin: The end of one journey is the beginning of another / End of one journey is the beginning of anotherHappynook, Tommy 05 May 2010 (has links)
My thesis serves two purposes: First, my research addresses what I have come to recognize as colonial misunderstandings of nuu-chah-nulth ha'wiih. My research and writing invoke new ways of thinking about nuu-chah-nulth people, leaders and knowledge. I accomplish this by writing conversationally and by including unedited interviews and poetry. All of which require readers to consider my research outside of their usual perspective. Second, my research responds to a cultural need to archive important family knowledge while providing the opportunity to define, for outsiders, who we are. The interviews archive, in part, the knowledge and teachings of a cha-cha-tsi-us-aht ha'wilth. My analysis of this information shows that while my family’s knowledge comes from a common source. We all interpret that knowledge in our own way. My research is important academically and politically because of its ability to convey knowledge that has not been simplified, appropriated or colonized for public consumption.
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