Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-14T17:34:23Z No. of bitstreams: 1 1997 - Isabelle Garcia Pina.pdf: 529649 bytes, checksum: d067fa62978c5955301ef929db0ee4f8 (MD5) / Made available in DSpace on 2017-02-14T17:34:23Z (GMT). No. of bitstreams: 1 1997 - Isabelle Garcia Pina.pdf: 529649 bytes, checksum: d067fa62978c5955301ef929db0ee4f8 (MD5) Previous issue date: 1997-09-30 / CNPq / This experimental work with Aedes aegypti L. (1762) was conducted in order to compare the effect of differents blood sources on feeding and egg production of mosquitoes? females. The blood-feeding device consisted of a plastic cage ? used for holding females during blood feeding ? with two lateral windows recovered with thin cloth to allow air exchange during feeding. In the top of the cage, there was an opening where the food?s container, recovered with silicone membrane, was attached. A larger vial filled with water at the initial temperature of 50 ?C was used over the apparatus to keep the food source warm (37,5 ? 0,5%). In each trial, three different treatments were tested: whole blood collected from mouse, bovine and human. To prevent coagulation, a 3,9% solution of sodium citrate was added to the blood. The control group was allowed to feed directly on human volunteers. The experiment was conducted in a climatic chamber, with temperature and humidity controlled at 28?0,5 ?C and 80?5%, respective values and the photoperiod of 12 hours of light and 12 hours of darkness. In each tested group, 30 females and 10 males mosquitoes were used. The females were allowed to feed during a period of 20-30 minutes and after that only the engorged females were transferred to another cages containing the oviposition sites. To evaluate the effect of different sources of blood on A. aegypti, the percentage of engorged females, pre-oviposition time and the number of eggs per engorged female were measured. Highest percentage of engorged females were among mosquitoes fed on human citrated blood (89%) and control (95%). The media oviposition on both treatments were 13,4 eggs for females fed on human citrated blood and 15,3 eggs for females of control group. The lots fed on mice and bovine citrated blood presented the following results respectively: 22,5% of engorged females and 9,2 eggs per female and 25,8% of engorged females and 10,3 eggs per female. / Foi comparado o efeito do sangue de camundongos, bovino e humano sobre o ingurgitamento e oviposi??o do mosquito Aedes aegypti L., 1762 (D?ptera:Culicidae). O aparato para alimenta??o artificial foi constitu?do por gaiolas de pl?stico, com 10 x 6 cm, com orif?cios laterais cobertos com tela de tule, e uma abertura superior, atrav?s da qual foi oferecido o sangue contido em uma c?lula montada em l?mina de microscopia com cola de silicone e membrana de silicone. O sangue foi mantido aquecido a 37,5 ? 0,5 ?C, durante o tempo de alimenta??o que variou de 20 a 30 minutos. O experimento foi conduzido dentro de uma c?mara climatizada, com temperatura de 28 ? 0,5 ?C, umidade relativa de 80 ? 5% e fotoperiodismo de 12 horas di?rias. Um total de 16 grupos de 30 f?meas de A. aegypti, com idades entre 4 a 7 dias, foram pr?-alimentados com solu??o a?ucarada de sacarose. Em cada grupo foram adicionados 10 machos para realiza??o de c?pula. O sangue de cada hospedeiro foi coletado assepticamente e adicionado de citrato de s?dio a 3,9%. O grupo controle foi alimentado diretamente em hospedeiro humano volunt?rio. Ap?s a alimenta??o, as f?meas ingurgitadas foram contadas e colocadas no interior de gaiolas com 50 x 50 x 50 cm, contendo frasco com ?gua limpa para oviposi??o. Foram registrados o n?mero de f?meas alimentadas em cada tratamento, o per?odo de pr?-postura e o n?mero m?dio de ovos por f?mea alimentada. Mosquitos alimentados com sangue de camundongos e de bovino tiveram performances de 22,5 e 25,8% de ingurgitamento e a postura foi em m?dia de 9,2 e 10,3 ovos por f?mea, respectivamente. F?meas alimentadas com sangue humano atrav?s da membrana ou diretamente no hospedeiro humano tiveram comportamentos diferentes com ?ndices de ingurgitamento de 89 e 95% e oviposi??o de, em m?dia, 13,4 e 15,3 ovos por f?mea, respectivamente. N?o houve varia??o no per?odo de pr?-postura (tr?s dias) entre os tratamentos realizados.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2018-05-11T13:46:52Z No. of bitstreams: 1 2010 - Renata Kazuko Sakai.pdf: 1267056 bytes, checksum: 688ad78c0490ec2ff8c2c641467143d0 (MD5) / Made available in DSpace on 2018-05-11T13:46:53Z (GMT). No. of bitstreams: 1 2010 - Renata Kazuko Sakai.pdf: 1267056 bytes, checksum: 688ad78c0490ec2ff8c2c641467143d0 (MD5) Previous issue date: 2010-02-22 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. / Artificial feeding of ticks can be used to study the relationship between vectors and pathogens, biological aspects, to test acaricides and to minimize the use of animals in scientific studies. The objectives of this study were artificially feeding through capillary tubes, females partially engorged of Rhipicephalus sanguineus previously fed on rabbits and dogs. In addition, the gain in weight of females and the influence of the technique in the biological aspects of non-parasitic phase of this species were also analyzed. Partially engorged females of R. sanguineus were obtained from infestation in rabbits and dogs in different intervals of initial weights and fed artificially for 24 hours to assess the best weight gains for the next stage. Female ticks with the best gains in weight were obtained on rabbits and dogs were separated into five groups of homogeneous weight, between 35 and 80mg and 21 to 50mg, respectively. The females were fixed in polystyrene tray with double-sided tape and fed artificially through capillary tubes for 6, 12, 24, 36 and 42 hours with citrated canine blood. After artificial feeding, ticks were again weighed to analyze the weight gain. Subsequently, they were fixed in Petri dishes and incubated at 27?1?C and humidity above 80%, in order to analyze the biological aspects. Control groups were obtained from natural fall of rabbits and dogs and the ticks were kept under the same conditions of temperature and humidity. For the statistical analysis, ANOVA and Tukey test were used for quantitative variables. In addition, the nonparametric Kruskal-Wallis was applied for qualitative variables, both with 5% of significance level. After 36 hours of artificial feeding there were difference statistically significant between groups, in relation to weight gain, for both females obtained from rabbits and dogs. Although the females of R. sanguineus had not been submitted to complete engorgement, the technique of artificial feeding of females derived from rabbits and dogs, no deleterious effects on the biological aspects of non-parasitic stage were observed. / A alimenta??o artificial de carrapatos pode ser utilizada para estudar a rela??o entre vetores e agentes patog?nicos, aspectos biol?gicos, para testar acaricidas e para minimizar a utiliza??o de animais em estudos cient?ficos. Os objetivos deste estudo foram alimentar artificialmente, por meio de tubos capilares, f?meas parcialmente ingurgitadas do carrapato Rhipicephalus sanguineus previamente alimentadas em coelhos e c?es. Al?m disso, foram analisados tamb?m o ganho de peso das f?meas e a influ?ncia da t?cnica nos aspectos biol?gicos da fase n?o parasit?ria desta esp?cie. F?meas parcialmente ingurgitadas de R. sanguineus foram obtidas de infesta??o em coelhos e c?es, em diferentes faixas de pesos iniciais e alimentadas artificialmente por 24 horas, para avaliar os melhores ganhos de peso para a pr?xima etapa. F?meas de carrapatos com os melhores ganhos de peso obtidas de infesta??o em coelhos e c?es, foram separadas em cinco grupos de peso homog?neo, pesando entre 35 a 80 mg e 21 a 50 mg, respectivamente. As f?meas foram fixadas em bandeja de isopor com fita adesiva dupla face e alimentadas artificialmente por meio de tubos capilares por 6, 12, 24, 36 e 42 horas, com sangue canino citratado. Ap?s alimenta??o artificial, os carrapatos foram pesados para avaliar o ganho de peso. Posteriormente, foram fixados em Placas de Petri e mantidos em estufa a 27?1?C e umidade superior a 80%, para avaliar os aspectos biol?gicos. Os grupos controles foram obtidos de queda natural de coelhos e c?es e mantidos nas mesmas condi??es de temperatura e umidade. Para an?lise estat?stica das vari?veis quantitativas foram utilizada an?lise de vari?ncia e teste de Tukey e para as vari?veis qualitativas foram utilizados o teste n?o param?trico Kruskal-Wallis, ambas com n?vel de signific?ncia de 5%. A partir de 36 horas de alimenta??o artificial foi observada diferen?a estat?stica significativa entre os grupos, em rela??o ao ganho de peso, tanto para f?meas obtidas de coelhos e de c?es. Embora as f?meas de R. sanguineus n?o tenham apresentado ingurgitamento total, a t?cnica de alimenta??o artificial de f?meas oriundas de coelhos e de c?es, n?o apresentou efeitos delet?rios sobre os aspectos biol?gicos da fase n?o parasit?ria.