Hydrolysis and solubilisation of organic material is known to be the rate limiting step during anaerobic digestion. In this study, the effect of pre-hydrolysis was investigated. The substrate had a composition of the following wastes streams: fish and slaughterhouse waste, cleaning waste, doggy meat, doggy sludge, doggy dry food, mink (fur), Norway slurry, SOR2-Industrial waste, and municipal solid waste (MSW) from households in Borås community.Analyses were made on two batches of experiments performed at two different mesophilic temperature conditions; 34 0C and 42 0C and with or without the addition of mesophilic sludge obtained from a waste water treatment plant. One of the substrate maintained the original carbon/nitrogen (C/N) ratio, and for the other substrate the carbon nitrogen ratio was then slightly increased by adding 7.98g of special shredded paper which contains 82% volatile solids (VS). The experiments were run for 7days with samples collected regularly after 0 day, 4 days, 5 days, 6 days and 7 days retention time. Chemical oxygen demand (COD), pH, total nitrogen, and volatile fatty acids (VFAs) as process indicators were analyzed.The results showed that at 42 0C the rate of hydrolysis was faster with a higher COD release and removal. The optimum retention time for hydrolysis was 4 days and any longer time caused some of the carbon to be lost to carbon dioxide. The effect of adding mesophilic sludge had no significant effect. An increased C/N ratio resulted in a balanced system with lower VFAs produced compared with that from the original substrate mixture. / <p>Acknowledgements: The authors are heartily thankful to their supervisor Ilona Sárvári Horváth whose guidance and support from the initial to the final level of this thesis work enabled us to gain a deeper understanding of the project.</p><p>We equally extend our sincere gratitude to the following persons who never ceased in helping until this work was complete.</p><p>Solmaz Aslanzadeh: For sharing her precious time and positive insight in coping with associated challenges of the project.</p><p>Hanson Jonas: Your assistance and patience over our laboratory experiments given the limited resources vis-a-vis students demand is quite reminding and saluted.</p><p>The support team at Sobacken Biogas plant: Your concern and timely assistance beginning from the supply of the substrate and the opportunity granted us to use your laboratory station greatly ensured results validation.</p><p>Program: MSc in Resource Recovery - Sustainable Engineering</p><p>Program: MSc in Resource Recovery - Industrial Biotechnology</p>