Master of Agribusiness / Department of Agricultural Economics / Jeffery R. Williams / Anaerobic digestion is an alternative solution to organic waste management that offers economic and environmental benefits. The Kawangware open air market in Kenya generates approximately 10 metric tons of organic waste per day as a result of farm produce sold at the market. Fresh fruits and vegetables sold at the market account for more than 80 percent of the organic waste. This organic waste is left uncollected, piling up and therefore becoming pollution to the environment. Instead, this waste can be processed by anaerobic digestion to produce energy, organic fertilizer and greenhouse gas credits. The main objective of this project is to help investors and members of Kawangware Waste Utilization Initiative (a waste management community based organization in the Kawangware area) answer the following questions: (a) Is it economically profitable to invest in an anaerobic digestion system to convert the market organic waste to methane and fertilizer? (b) Is it economically profitable to burn the methane to generate electricity? To answer these questions, the study examines the costs and returns of producing methane, electricity, and fertilizer from organic waste under various scenarios using net present value, internal rate of return and payback period analysis techniques. Three production conditions under various scenarios using the anaerobic digester are examined. The conditions include: (a) Production of methane and organic fertilizer. (b) Production of methane, organic fertilizer, and carbon credits. (c) Production of electricity, organic fertilizer, and carbon credits. From these three production conditions examined, production of methane, organic fertilizer and carbon credits had the highest net present value of $332,610, internal rate of return of 21.4%, and the shortest payback period of 7.9 years. If carbon credits could not be sold the next best alternative would be production and selling of methane and organic fertilizer which has a net present value of $246,752, internal rate of return of 19%, and a payback period 9.2 years.

Thesis (M.Tech.: Biotechnology)-Dept. of Biotechnology, Durban Institute Of Technology, 2005 xxiii, 172 p. : ill. ; 30 cm / The provision of safe and sanitary water is a constitutional right and above all, a necessity of life. As a result of the rapid urbanisation and the past policies of apartheid, a large population of South Africa dwell in informal settlements, where there is very little hope of development, as the government does not possess the resources that are necessary for a full-scale sanitation programme. Therefore, on-site treatments have been considered to provide sanitation in these dense peri-urban areas. The anaerobic baffled reactor (ABR) is one such sanitation system. This reactor utilises the phenomenon of anaerobic digestion to degrade substrates. One of the major disadvantages of any anaerobic treatment processes is the extreme sensitivity of the bacterial communities, thus inducing slow recovery rates following toxic shocks. Therefore, an understanding of these microbial consortia is essential to effectively control, operate and optimise the anaerobic reactor. Fluorescence in situ hybridization, 4’,6-diamidino-2-phenylindole (DAPI) staining and DNA sequencing techniques were applied to determine the microbial consortium, as well as their reactions to daily operating conditions. With an understanding of these populations and their responses to perturbations within the system, it is possible to construct an anaerobic system that is successful in its treatment of domestic wastewater. In situ hybridizations were conducted for three operating periods, each characterised by specific flow rates. Results showed Eubacterial population dominance over the Archaeal population throughout both of the operating periods investigated. However, these cells cumulatively consisted of 50% of the total biomass fraction, as determined by DAPI staining. Group-probes utilised revealed a high concentration of fermentative acidogenic bacteria, which lead to a decrease in the pH values. It was noted that the ABR did not separate the acidogenic and methanogenic phases, as expected. Therefore, the decrease in pH further inhibited the proliferation of Archaeal acetoclastic methanogens, which were not present in the second operating period. DNA sequencing results revealed the occurrence of the hydrogenotrophic Methanobacterium and Methanococcus genera and confirmed the presence of Methanosarcina. Sequencing of the bacterial DNA confirmed the presence of the low G+ C Gram Positives (Streptococcus), the high G+C Gram Positives (Propionibacterium) and the sulfate reducing bacteria (Desulfovibrio vulgaris). However, justifications were highly subjective due to a lack of supportive analytical data, such as acetate, volatile fatty acids and methane concentrations. Despite this, findings served to add valuable information, providing details on the specific microbial groups associated with ABR treatment processes.

Submitted in partial fulfillment of academic requirements for the degree of Masters of Technology: Department of Chemical Engineering, Durban University of Technology, 2009. / The primary objective of this project was to establish a screening protocol which could be used to access high strength/toxic effluent for toxicity and degradability prior to being disposed in wastewater treatment works. The serum bottle method (materials and method section) is simple, makes use of small glass vials (125 mℓ-volume were used in this research) which do not require any stirring nor feeding device or other engineered tool: a serum bottle is sealed immediately after all components are poured inside and thereafter conducted in a batch mode and occasionally shaken to ensure adequate homogenisation of the components. The only variables which are regularly measured are the volume of biogas produced and gas composition. The two assays, originally developed by Owen et al. (1979) to address the toxicity and the biodegradability have been combined in a single test called AAT, Anaerobic Activity Test, which enables one to assess simultaneously the inhibitory effect on the methanogenic biomass and the biodegradability of the test material as well as the ability of the biomass to adapt to the test material and therefore to overcome the initial inhibition. The screening protocol is illustrated in Annexure A. The protocol consists of a sequence of assays which employ the serum bottle methodology. A first step of the procedure is aimed at rapidly estimating whether the effluent is potentially toxic to the methanogenic biomass and in what concentration. The second step is a more extensive screening, aimed at precisely characterising the toxicity of the effluent, the extent of biodegradation that can be achieved, as well as at establishing whether a potential for adaptation of the biomass exists upon exposure. If the sample passes the screening stage, the same serum bottle method will be used to conduct a series of batch co-digestion experiments aimed at evaluating a convenient volumetric ratio between the test material and the readily biodegradable substrate. Finally, a laboratory-scale codigestion trial could simulate the full-scale process, thus enabling the selection of appropriate operating conditions for the start-up of the full-scale implementation. This the protocol has been used to assess the amenability to be anaerobically (co)digested of four industrial effluents, i.e. size and distillery effluents which are classified as high strength and scour and synthetic dye effluents classified as toxic. From the biodegradability and toxicity assays the following conclusions were drawn. The size and distillery effluent were found to be ii degradable at 32 g COD/ℓ and 16 g COD /ℓ concentrations respectively. Concentrations higher than these stipulated above were found inhibitory. Scour effluent was found to be recalcitrant at all concentration tested and synthetic dye was 100 % degradable at 0.12 g COD/ℓ and lower and highly inhibitory at concentration higher than 1.1 g COD/ℓ. Co-digestion experiment using serum bottle AAT method were undertaken between effluents i.e. size + distillery, size + scour, distillery + synthetic dye in an attempt to verify whether the digestion performance benefits from simultaneous presence of the two substrates. The volumetric ratios between the effluents were 1:1, 1:2, 2:1. The presence of two mixtures in the case of size and distillery had better methane production compared to individual substrate i.e. size or distillery separate. The mixture with volumetric flow rate ratio of 2:1 (size: distillery) was preferable in terms of process performance as it had highest COD removal compared to the other mixtures /ratios and individual substrates. The mixture of size and scour (2:1) had highest degradation percentage compared to other ratios but not high enough to qualify as degradable (less than 50 %). The mixture of distillery and synthetic dye had the same pattern with ratio of 2:1 giving the best COD conversion. The pattern than can be drawn from the degradability of mixtures is: the degradability of mixtures increase with the increasing amount of the most biodegradable compound/effluent in the mixture. Serum bottle results provided the detailed information regarding the safe operating parameters which should be used during the starting point for the larger scale investigation i.e. lab-scale investigations. The lab scale investigations were conducted primarily to validate screening and monitor how the digestion progresses and also to provide data for future project i.e. pilot plant investigation. Other effluents i.e. scour and synthetic dye and their co-digestion mixture were excluded from the lab-scale investigations since they were found to be non- biodegradable i.e. their COD conversion was less the 50 % in the screening protocol. Due to time constrains and other technical difficulties in the laboratory, the co-digestion of size and distillery mixture trials we not conducted on the laboratory scale. Laboratory-scale digestion trials showed that the best organic loading rate for distillery effluent in terms of reactor performance and stability was 1.0g COD/ℓ with efficiency of about 45 %, and for size was 2.0g COD/ℓ with an efficiency of 40 %. The efficiencies obtained in both effluents trials could be greatly improved by acclimation; however these results showed that the digestion of these effluents on the bigger scale is possible. / Water Research Commission

Burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic in Northern Australia and Southeast Asia. Melioidosis can present with acute, chronic and latent infections and can relapse several months or years after initial presentation. Currently not much is known about the ways in which B. pseudomallei can persist within the host, although it has been speculated that the ability to survive within an anaerobic environment will play some role. B. pseudomallei is able to survive anaerobically for extended periods of time but little is known about the molecular basis of anaerobic respiration in this pathogenic species. Bioinformatic analysis was used to determine the respiratory flexibility of both B. pseudomallei and B. thailandensis, identifying multiple genes required for aerobic and anaerobic respiration, and molybdopterin biosynthesis. Using B. thailandensis as a model organism a transposon mutant library was created in order to identify genes required for anaerobic respiration. From this library one transposon mutant was identified to have disrupted moeA, a gene required for the molybdopterin biosynthetic pathway. This B. thailandensis transposon mutant (CA01) was unable to respire anaerobically on nitrate, exhibiting a significant reduction in nitrate reductase activity, altered motility and biofilm formation, but did not affect virulence in Galleria mellonella. It was hypothesised that the reduction in nitrate reductase activity was contributing to the phenotypes exhibited by the B. thailandensis moeA transposon mutant. To determine whether this was true an in-frame narG deletion mutant was created in B. pseudomallei. Deletion of B. pseudomallei narG (ΔnarG) resulted in a significant reduction in nitrate reductase activity, anaerobic growth, motility and altered persister cell formation, and but did not affect virulence in G. mellonella or intracellular survival within J774A.1 murine macrophages. This study has highlighted the importance of anaerobic respiration in the survival of B. thailandensis and B. pseudomallei.

The production of pulp and paper is associated with the generation of large quantities of wastewater that has to be purified to avoid severe pollution of the environment. Wastewater purification in pulp and paper mills combines sedimentation, biological treatment, chemical precipitation, flotation and anaerobic treatment, and the specific combination of techniques is determined by the local conditions. Wastewater treatment generates large volumes of sludge that after dewatering can be incinerated and thus used for bio-energy production. Sludge is currently viewed as biofuel of poor quality due to its high water content, and some mills treat it solely as a disposal problem. Two strategies have been identified as feasible options to improve the energy efficiency of sludge management. One is drying using multi-effect evaporation followed by incineration. The other is anaerobic digestion of the wet sludge to produce methane. This thesis explores the energy balances of sludge management strategies in pulp and paper mills with special focus on anaerobic digestion. The first part consists of a system analysis, used to evaluate some wastewater treatment processes and sludge management, and the second part of empirical studies of anaerobic digestion of pulp and paper mill sludge. It was shown that the use of energy for aeration in aerobic biological treatment should be kept to the minimum required for acceptable quality of the processed water. Additional aeration for reduction of the generated sludge will only result in reduced energy generation in a subsequent methane generation stage. In the second part of the thesis, it is shown that anaerobic digestion is a feasible option for sludge management as it leads to production of high value biogas. Co-digestion with grass silage, cow/pig manure or municipal sewage sludge should then be used to counteract the low nitrogen content of pulp and paper mill sludge.

Surrogate organisms have been used to study highly pathogenic organisms, or organisms that cannot be cultured in the laboratory. Surrogates are selected based on multiple similarities to the pathogen, such as morphology, genome size and structure, and environmental characteristics. This dissertation utilized MS2 coliphage as a surrogate for norovirus and Ebola virus in three environments. MS2 is an icosahedral, single-stranded RNA bacteriophage. It is a male-specific coliphage that infects the bacteria Escherichia coli. Its properties, such as morphology and survival in the environment, have been likened to those of many enteric viruses. Because of this, it has been used as a surrogate for pathogenic enteric viruses for disinfection testing on surfaces, in water and in food; modeling the movement and survival of pathogens in different environments; and transfer properties from surfaces. This dissertation utilized MS2 as a surrogate in three different studies. In the first, MS2 is used as a surrogate for human enteric viruses in irrigation canals to predict the re-suspension of pathogenic viruses from bed sediment into overlying irrigation water using a flume to re-create field conditions in the laboratory. MS2 re-suspension into the overlying water was characterized at varying flow rates and velocities using two sediment types. Its overall re-suspension was not statistically significantly different (p > 0.05) between flow rates. The additional studies in this dissertation used MS2 as a surrogate for Ebola virus in human waste. Ebola virus is a BSL-4 organism that is spread through direct contact with bodily fluids. It is found in bodily fluids in concentrations between 10^5.5 and 10⁸ genome copies per milliliter. In the first study using MS2 as a surrogate for Ebola virus, efficacies of four disinfectants were tested using 10¹² PFU of MS2 in one liter containing 2.25% (w/v) organic matter at three contact times (1, 15 and 30 minutes). The purpose of this study was to assess the disinfectants on reducing virus in waste before toilet flushing. Peracetic acid and quaternary ammonium formulation were found to reduce the concentration of MS2 in the toilet bowl the fastest (within one minute) with the greatest reduction (2.26 and 1.99 log₁₀), when compared with the other disinfectants. Reductions observed from hydrogen peroxide were significantly less than those from peracetic acid and quaternary ammonium (p < 0.05). The contamination of restroom surfaces by MS2 was also evaluated after toilet flushing with and without disinfectant treatment. All four disinfectants were found to significantly reduce the viral concentrations on fomites after 15 minutes of contact (p < 0.05). Despite disinfectant use, three sites were contaminated in 100% of trials (N = 18). These were the toilet bowl rim, the toilet seat top and underside. The final study evaluated the inactivation of MS2 and several other viruses by thermophilic and mesophilic anaerobic digestion. Little information is available on the influence of the wastewater treatment process, specifically anaerobic digestion, on emerging viruses, such as Ebola virus. It is important to evaluate this process due to the environmental disposal and discharge of wastewater and solids into the environment. All viruses were recoverable after mesophilic digestion (reductions from 1.8-6.6 log₁₀ per mL), except the lipid-containing bacteriophage Φ6. Thermophilic digestion inactivated all viruses significantly (p = 0.0011) more than mesophilic digestion. The reductions by thermophilic digestion ranged from 2.8-7.1 log₁₀ per mL. The inactivation between the initial concentration and both digestion types was statistically significant (p = 0.007).

We initially studied the treatment of acid rock drainage using a sulfate-reducing bioreactor with zero-valent iron as the electron donor. The results demonstrate that this electron donor can serve as the sole exogenous slow-release electron donor to drive sulfate reduction over 400 operational days at short HRTs (1-3 days). The synthetic acid rock drainage contained high heavy metal concentrations (up to 50 mg/L of copper) and pH values ranging from 3.0 to 7.0. Treatment of this acid rock drainage efficiently removed Cu, Cd and Pb (>99.7%) and increased pH to circumneutral values (7.3-7.7). Elemental analysis indicated that formation of insoluble metal sulfides was responsible for the effective metal removal in the zero valent iron columns. In the second study, three inoculated columns containing anaerobic granular sludge were fed a synthetic medium containing H₂SO₄ and Cu²⁺ during the experimental period of 4 months. Algae biomass promoted 80% of sulfate removal (12.7 mg SO₄²⁻ d-1), enabling near complete Cu removal (>99.5 %), and alkalinity generation, raising the effluent pH to 6.5. In the algae amended columns Cu²⁺ was precipitated with biogenic H2S produced by sulfate reduction. Whole cell algae and lipid extracted algae biomasses were both shown to be effective e-donors in driving sulfate reduction of ARD, thus enabling the precipitation and removal of Cu²⁺. The precipitate retained in the columns was composed mostly of insoluble copper sulfide formed from the biogenic sulfide, as shown by sequential extraction and X-ray diffraction. In the third study, several pretreatments, i.e., thermal, chemical, sonication and combinations thereof, that enhance anaerobic biodegradability of Chlorella protothecoides biomass were evaluated. The results demonstrate that anaerobic digestion of pretreated Chlorella protothecoides biomass generates energy-rich methane and recovers nitrogen nutrients. Sonication of algal biomass under optimized conditions provided a significant increase in the methane yield (327 mL STP CH₄ g⁻¹ VS) compared to untreated algae (146 mL STP CH₄ g⁻¹ VS), as demonstrated in anaerobic digestion experiments incubated for 41 days. In contrast, thermal pretreatment provided only a moderate increase of the methane yield and alkaline treatment led to a decrease of the methane yield compared to the untreated algal biomass. Additionally, sonication treatment provided a 4.1-fold increase in the release of ammonia nitrogen during anaerobic digestion of the algal biomass. In the fourth study, the nutrient recovery and biogas generation from the anaerobic digestion of waste biomass from algal biofuel production was investigated. Anaerobic digestion of whole cell and lipid extracted Chlorella sorokiniana-1412 released 48.1 and 61.5% of the total algal nitrogen as NH₄⁺-N, and 87.7 and 93.6% of the total algal P as soluble P, respectively. The biochemical methane potential, quantified through the methane yield of whole cell algae and lipid extracted algae, was 0.298 and 0.253 L methane/g algal volatile solids, respectively. The conversion of lipid extracted algae and whole cell algae biomasses to methane was very similar (38 and 41% on a COD basis, respectively), indicating that the energy yield was not significantly lowered by extraction of the lipid fraction (which accounted for 9% of algal dry weight). Sonication improved the access of hydrolytic enzymes to algal biopolymers, compensating in part for the energy lost due to lipid extraction. The above results demonstrate that algal waste from the biodiesel industry has the potential to be recycled through anaerobic digestion into valuable nutrients and energy. These studies indicate that zero valent iron and algae biomass are promising reactive materials for the treatment of acid rock drainage in sulfate-reducing permeable reactive barrier systems. Additionally, to promote algae cultivation for the biodiesel industry, the anaerobic digestion of algae residues can generate nutrients and energy, making algae cultivation more fiscally attractive.

Thesis (MSc)--Stellenbosch University, 2000. / ENGLISH ABSTRACT: A severely polluted industrial effluent is generated by the local gelatinmanufacturing industry. Due to increasingly stringent restrictions on discharge qualities enforced by the National Water Act of 1998 and National Environmental Management Act of 1998, as well as increasing trade-effluent charges implemented via the Local Municipal Bylaws, the industry is compelled to consider a system to pre-treat the polluted effluent. A study was undertaken to examine the viability of anaerobic treatment of the gelatin-manufacturing effluent, since the anaerobic digestion technology is well recognised for the high success rate in the treatment of high-strength, complex wastewaters. Various laboratory and pilot-scale studies were done, using different hybrid Upflow Anaerobic Sludge Blanket (UASB) and contact designs. Two mesophilic laboratory-scale hybrid UASB digester designs, fitted with polyethylene (AD-1) and polyurethane (AD-2), performed well at a hydraulic retention time (HRT) of 1.0 d. Chemical oxygen demand (COD) removal efficiencies of up to 90% (avg. 53%) for AD-1 and 83% (avg. 60%) for AD-2 at organic loading rates (OLR) of 9.56 and 4.62 kg COD.m-3.d-1, respectively, were obtained. High sulphate (S04) removal efficiencies of up to 96% (avg. 86%) for AD-1 and 98% (avg. 82%) for AD-2 were also achieved, respectively. A maximum total solid (TS) removal of 65% (avg. 25%) for AD-1 and 62% (avg. 28%) for AD-2 was reported. An average methane content of 80% (AD-1) and 79% (AD-2) with average methane yields per COD removed of 2.19 and 1.86 m3. kg CODremoved.df-o1r AD-1 and AD-2 were found, respectively. When the same digesters (AD-1 and AD-2) were combined in a muItiphase series configuration, a total COD removal efficiency of up to 97% (avg. 80%) at an OLR of 8.32 kg COD.m-3.d-1,was achieved. Excellent total S04 removals of 96% (avg. 69%) were accomplished. Up to 82% TS (avg. 29%) was also removed during this study and the biogas consisted of 89% methane (avg. 79%). For this multi-phase combination up to 92% volatile fatty acids (VFA) (avg. 48%) were removed, indicating possible selective phase separation of the respective fatty acid producing/utilising bacterial populations. The use of a laboratory-scale UASB bioreactor with recirculation, resulted in COD removal efficiencies of up to 96% (avg. 51%) at an HRT of 3.0 d, and 95% (avg. 54%) at a HRT of 1.0 d. Low performances were generally found, with average S04 and TS removals of 59% (max. 97%) and 26% (max. 67%), respectively at an HRT of 1.0 d. The biogas production was very low throughout the study (0.05 - 0.63 I,d-1 ). A pilot-scale UASB reactor (300 I) was constructed and performed satisfactory with a 58% average COD removal and maximum of 96%. S04 and TS removals up to 96% (avg. 44%) and 93% (avg. 63%), respectively, were obtained. The methane content of the biogas was 85%. The pilot-scale studies were conducted under actual field conditions, where various shock and organic loads had to be absorbed by the system. The pilot-scale contact configuration (300 I) did not perform satisfactory as a result of continuous blockages experienced in the feed and recirculation lines. Maximum COD, S04, VFA and TS removal efficiencies of 41% (avg. 27%), 62% (avg. 41%), 64% (avg. 27%) and 39% (avg. 21%), respectively, were obtained. The results of all the studies indicated acceptable COD removals with increasing OLR's. Indications of the presence of active methanogenic and sulphate-reducing bacterial populations were apparent throughout the studies. One possibility for the successful start-up and commissioning of the anaerobic reactors was the use of a well-adjusted biomass, which consisted of highly selected and adapted microbial consortium for the specific gelatinmanufacturing effluent. It was clear from this study that gelatin-manufacturing effluent can be treated successfully, especially with the use of the UASB design. A welldefined data base was constructed which could be of great value for further upscaling to a full-scale digester. / AFRIKAANSE OPSOMMING: 'n Hoogs besoedelde industriele uitvloeisel word gegenereer deur die plaaslike gelatien-vervaardigings industrie. As gevolg van toenemende streng beperkings op die kwaliteit van uitvloeisels wat bepaal word deur die Nasionale Water Wet van 1998 en Nasionale Omgewings Bestuurs Wet van 1998, asook toenemende munisipale heffings wat geimplementeer word via Plaaslike Munisipale Wette, word die industrie verplig om die uitvloeisel vooraf te behandel. 'n Studie is onderneem om die lewensvatbaarheid van anaërobe behandeling van gelatien-vervaardigings uitvloeisel te ondersoek, aangesien anaërobe verterings tegnologie alombekend is vir die goeie sukses behaal in die behandeling van hoë-sterkte, komplekse uitvloeisels. Verskeie laboratorium- en loods-skaal studies is gedoen, met verskillende hibried Opvloei Anaërobe Slykkombers (OAS) en kontak ontwerpe. Goeie werksverrigting was verkry by 'n hidroliese retensie tyd (HRT) van 1.0 d met twee mesofiliese laboratorium-skaal hibried OAS verteerder ontwerpe wat uitgevoer was met poli-etileen (AD-1) en poli-uretaan (AD-2) materiaal. Chemiese suurstof behoefte (CSB) verwyderings van so hoog as 90% (gem. 53%) vir AD-1 en 83% (gem. 60%) vir AD-2 by organiese ladingstempo's (OLT) van 9.56 en 4.62 kg CSB.m-3.d-1,was onderskeidelik verkry. Hoë sulfaat (S04) verwyderings van tot 96% (gem. 86%) vir AD-1 en 98% (gem. 82%) vir AD-2 was ook onderskeidelik verkry. 'n Maksimum totale vaste stof (TVS) verwydering van 65% (gem. 25%) vir AD-1 en 62% (gem. 28%) vir AD-2 is gerapporteer. 'n Gemiddelde metaan inhoud van 80% (AD-1) en 79% (AD-2) met 'n gemiddelde metaan opbrengs per CSB verwyder van 2.19 en 1.86 m3.kg CSBverwyder.dv-i1r AD-1 en AD-2, was onderskeidelik gevind. Met die aanwending van dieselfde twee verteerders (AD-1 en AD-2) in 'n series gekoppelde multi-fase konfigurasie, is 'n totale CSB verwydering so hoog as 97% (gem. 80%) verkry by 'n OLT van 8.32 kg CSB.m-3.d-1. Uitstekende totale S04 verwydering van 96% (gem. 69%) is behaal. Tot 82% TVS (gem. 29%) was vewyder gedurende die studie en die biogas het uit 89% metaan (gem. 79%) bestaan. Vir die multi-fase kombinasie is 'n maksimum van 92% vlugtige vetsure (WS) (gem. 48%) verwyder, wat dui op die moontlike skeiding van selektiewe fases van die onderskeie vetsuur produserende/verbruiker bakteriële populasies. CSB verwydering van tot 96% (gem. 51%) by 'n HRT van 3.0 d en 95% (gem. 54%) met 'n HRT van 1.0 d was verkry, tydens die gebruik van In laboratorium-skaal OAS bioreaktor met hersirkulasie. Lae werksverrigting was oor die algemeen waargeneem, met gemiddelde S04 en TVS verwyderings van 59% (maks. 97%) en 26% (maks. 67%) by In HRT van 1.0 d. Die biogas produksie was baie laag gedurende die studie (0.05 - 0.63 I,d-\ In Loods-skaal OAS verteerder was opgerig en bevredigende resultate was verkry met In gemiddeld van 58% CSB verwydering en maksimum van 96%. S04 en TVS verwyderings so hoog as 96% (gem. 44%) en 93% (gem. 63%) is onderskeidelik verkry. Die metaan inhoud van die biogas was 85%. Die loods-skaal studie was uitgevoer gedurende ware veld kondisies, waartydens verskeie skok en organiese ladings deur die sisteem geabsorbeer is. Die loods-skaal kontak konfigurasie (300 I) het nie bevredigende resultate getoon nie, as gevolg van voortdurende blokkasies wat ondervind is in die toevoer en hersirkulasie pype. Maksimum CSB, S04, WS en TVS verwyderings van 41% (gem. 27%), 62% (gem. 41%), 64% (gem. 27%) en 39% (gem. 21%) was onderskeidelik verkry. Die resultate van al die studies het aanvaarbare CSB verwydering aangedui by toenemende OLT's. Indikasies van aktiewe metanogene en sulfaat-reduserende bakteriële populasies was ook teenwoordig gedurende die studies. Die suksesvolle aansit-prosedure en begin van die anaërobe verteerders kan toegeskryf word aan die gebruik van In goed aangepaste biomassa, wat uit hoogs selektiewe en aangepaste mikrobiese populasies vir die spesifieke uitvloeisel bestaan. Hierdie studie het getoon dat gelatien-vervaardigings uitvloeisel suksesvol met die OAS ontwerp behandel kan word. In Goed gedefinieerde data basis kan voorsien word, wat van groot waarde sal wees vir verdere opgradering na In volskaalse verteerder.

Thesis (MSc)--Stellenbosch University, 2015. / ENGLISH ABSTRACT: Acid mine drainage (AMD) is wastewater generated by mine and industrial activity with typically high heavy metal and sulphur content potentially resulting in toxic wastewater upon exposure to dissolved oxygen, water and micro-organisms. Due to the hazardous consequences of untreated AMD, treatment methods such as semi-passive biotic treatments, including constructive wetlands and microbial bioreactors were developed. Microbial bioreactors rely on suitable carbon sources such as ethanol, grasses and manure and the creation of anaerobic conditions for the reduction of sulphate, chemical oxidizable organic matter (COD) and to neutralise pH. Domestic wastewater sludge has also been identified as an economical and readily available carbon source that allows the treatment of both AMD and domestic wastewater. A synthetic medium simulating the COD and the biological degradable organic matter (BOD) of domestic wastewater sludge was formulated to exclude variations in the evaluation of domestic wastewater sludge as carbon source in the treatment of AMD. Firstly the BOD and COD of anaerobic domestic wastewater sludge was determined and used as parameters in the formulation of the synthetic medium. A ratio of 1:1 AMD: synthetic domestic wastewater sludge (SDWWS) was the optimum ratio in terms of sulphate and COD removal. Secondly, medical drip bags were used as anaerobic bioreactors to determine the microbial diversity in AMD treated with SDWWS using different variables. Data analyses from next generation sequencing showed that Chlorobium spp. dominated the 90 d pioneer trials at relative percentages of 68 % and 76 %. Transmission electron microscopy (TEM) images and the bright green colour of the liquid contents confirmed the data analyses. Sulphates and COD were removed at > 98 % and > 85 %, respectively. A shorter incubation time was investigated in the 30 d pioneer trial. Chlorobium spp. was dominant, followed by Magnetospirillum spp. and Ornithobacterium spp. The liquid content changed to a dark brown colour. COD and sulphate concentrations were reduced by 60.8 % and 96 %, respectively, within 26 d, after which a plateau was reached. The effect of an established biofilm in the bioreactors showed that Chlorobium spp. also dominated approximately 62 %, in comparison to the 36 % in the 30 d pioneer trial. A sulphate and COD reduction of 96 % and 58 %, respectively, was obtained within 26 d and the liquid content was the same colour as in the 30 d pioneer trial. It is possible that brown Green sulphur bacteria were present. Therefore, although Chlorobium spp. was present at a higher percentage as in the 30 d pioneer trial, the removal of COD and sulphate was similar. During the 30 d trials a white precipitant formed at the top of the bioreactors, consisting primarily of sulphate and carbon that was also indicative of the presence of Chlorobium spp. Incubation at reduced temperature reduced sulphates by only 10 % and COD by 12 % after 17 d, followed by a plateau. Ornithobacterium spp. dominated in the first trial and Magnetospirillum spp. in the second trial. / AFRIKAANSE OPSOMMING: Suur mynwater (SMW) is afvalwater wat deur die myn- en industriële bedryf gegenereer word en bevat kenmerklik hoë konsentrasies swaar metale en swawel wat potensieel in toksiese afvalwater omskep kan word indien blootgestel aan opgelosde suurstof, water en mikro-organismes. Die skadelike gevolge wat blootstelling aan onbehandelde SMW mag hê, het gelei tot semi-passiewe behandelinge wat vleilande en mikrobiese bioreaktors insluit. Mikrobiese bioreaktore maak staat op n geskikte koolstofbron soos etanol, grasse en bemesting en die skep van ‘n anaerobiese omgewing vir die verwydering van sulfate en chemies oksideerbare organiese material (CSB), asook die neutralisering van pH. Huishoudelike afvalwaterslyk is ook uitgewys as ‘n ekonomies geskikte en algemeen beskikbare koolstofbron wat die behandeling van beide SMW en huishoudelike afvalwater toelaat. ‘n Sintetiese medium wat die CSB en biologies afbreekbare organiese materiaal (BSB) van huishoudelike afvalwater slyk naboots is geformuleer om die variasies in die evaluasie van huishoudelike afvalwater slyk as koolstofbron vir die behandeling van SMW, uit te sluit. Eerstens is die BSB en die CSB van huishoudelike afvalwater slyk bepaal en gebruik as n maatstaf vir die formulering van die sintetiese medium. ‘n Verhouding van 1:1 sintetiese huishoudelike afvalwater slyk (SDWWS) en SMW is optimaal ratio i.t.v. die verwydering van sulfate en CSB. Tweedens is mediese dripsakkies as anaerobiese bioreaktore gebruik om die mikrobiese diversiteit in SMW, wat met SDWWS behandel is, te bepaal deur verskeie veranderlikes te gebruik. Tweede generasie DNA-volgorde bepalingstegnieke is gebruik en data analises het gewys dat Chlorobium spp. die 90 d pionier toetslopie domineer met relatiewe persentasies van 68 % en 76 %. Transmissie elektron mikroskopie fotos en die helder groen kleur van die dripsakkies se vloeistof inhoud het die data analises bevestig. Die sulfate en CSB inhoud is onderskeidelik met > 98 % en > 85 % verminder. ‘n Korter behandelingstydperk is ondersoek met n 30 d pionier toetslopie. Chlorobium spp. was dominant, gevolg deur Magnetospirillum spp. en Ornithobacterium spp. Die vloeistof inhoud het na ‘n donker bruin kleur verander. Die CSB en sulfaat konsentrasies is met 60.8 % en 96 % onderskeidelik verminder na 26 dae waarna ‘n plato bereik is. Die effek van ‘n reeds bestaande biofilm in die bioreaktore het gewys dat Chlorobium spp. ook gedomineer het teen ‘n relatiewe persentasie van 62 % in vergelyking met die 36 % in die 30 d pionier toetslopie. ‘n Vermindering in sulfate en CSB van 96 % en 58 % is onderskeidelik is bereik binne 26 d en die vloeistofinhoud was dieselfde kleur as die bioreaktore in die 30 d pionier toetslopie. Dit is moontlik dat die bruin Groen swawel bakterieë teenwoordig was. Daarom, ondanks ‘n groter teenwoordigheid van die Chlorobium spp. teen ‘n relatiewe persentasie in vergelying met die 30 d pionier toetslopie, was die verwydering van CSB en sulfate soortgelyk. Tydens die 30 d toetslopies het ‘n wit neerslag aan die bokant van die bioreaktore gevorm wat hoofsaaklik uit sulfaat en koolstof bestaan het wat ook ‘n aanduiding van die teenwoordigheid van Chlorobium spp. is. ‘n Toetslopie wat by laer temperature uitgevoer is kon die sulfate en CSB met slegs 10 % en 12 % onderskeidelik verminder nadat ‘n plato na 17 d bereik is. Ornithobacterium spp. het die eerste toetslopie gedomineer waar Magnetospirillum spp. die tweede toetslopie gedomineer het.

Thesis (MScEng (Process Engineering))--University of Stellenbosch, 2005. / The thesis reports the investigation of various distillery processes and wastewater streams. The aim was to evaluate the processes and thereafter design interventions for improved wastewater treatment at the respective distilleries. An integrated environmental approach was adopted based on the principle that prevention of pollution is the preferred option and end-of-pipe treatment the least favoured option. As such, feed material to the processes was studied to determine whether some of the components that are not required in the distillation process could in fact be removed prior to entering the system. The results indicate that organic constituents such as phenol and tartaric acid could be removed using physico-chemical and biological treatment methods. The treatment of effluent was studied using an Upflow Anaerobic Sludge Blanket (UASB) set-up to determine the reduction in Chemical Oxygen Demand (COD) in the wastewater. Thereafter the UASB treated effluent was exposed to aeration for further treatment. Summary of conclusions • Pretreatment of wine feed material with calcium hydroxide is effective in removal of 98% tartaric acid, 30% COD and a total phenol content of 57%. • Bio-augmentation results showed that the soil inoculum was the most effective treatment method with reductions of 61% COD at a temperature of 30°C, tartaric acid removal of 98% at the same temperature and 25% reduction in total phenol at 26°C. • UASB was effective with soil inoculum and removed approximately 90% of COD although operational problems were experienced and hindered the operation of the plant. • Aeration of UASB effluent further reduced the COD by a further 60% with a total COD reduction of 96% after both UASB and aeration treatment. • Effective reduction of total phosphorus by 70% and the total phenol content by 80% was achieved by UASB treatment followed by aeration.