Xylose is an unusual nectar sugar found in the nectar of Protea and Faurea (Proteaceae). Since nectar composition is an important floral characteristic in plant strategies for ensuring reproductive success, the unexplained presence of xylose in Protea nectar prompted this study of the interaction between pollinators and Protea species. Among pollinators that visit Proteaflowers in the south-western Cape Floral Kingdom, South Africa, insects and birds show an aversion to, and are poor assimilators of, xylose, whereas rodents such as Namaqua rock mice Aethomys namaquensis are the only pollinators so far shown to consume xylose willingly, and are able to obtain metabolic energy from this sugar. Mammalian tissues are not capable of catabolizing xylose efficiently, but certain gastrointestinal microflora are, through a process of fermentation which produces short-chain fatty acids used by host animals in oxidative metabolism. I explored mechanisms enabling Aethomys namaquensis to utilize xylose, in particular, the role of resident intestinal microflora in this process. Chapter One discusses pollination syndromes and the definitions thereof, mammal pollination, with particular reference to Australia and South Africa, and explains the rationale behind the questions addressed in this thesis. To assess xylose utilization in wild-caught mice with and without their natural gastrointestinal microflora, an antibiotic treatment was developed (Chapter Two). The veterinary antimicrobial agent, Baytril 10% oral solution, was found to be effective in significantly reducing gut microflora in animals on a four-day treatment protocol. The protocol developed here reduced the gut microflora sufficiently for subsequent experiments comparing xylose utilization in mice with and without intact microflora. Xylose utilization was assessed using 14C-labelled xylose (Chapter Three). Rock mice were caught during Protea humifloraflowering and non-flowering seasons, and fed 14C-labelled xylose. Exhaled CO 2 and excreted urine and faeces were collected, and label recovery determined. These experiments showed that xylose-utilizing bacteria in the rock mouse gut are very important for xylose utilization. More efficient xylose utilization during the flowering season suggests that this component of the gut microflora is inducible. Culturable gut microflora were then isolated from rock mouse faecal and caecal samples, assessed for xylose utilization to identify positive xylose-fermenters and classified by 16S rRNA based taxonomy (Chapter Four). Faecal isolates were Lactobacillus murinus and Enterococcus faecium, and caecal isolates were three Bacillus species, Shigella boydii, one Arthrobacter species and two fungal isolates from Aspergillus and Penicillium genera. The types and concentrations of short-chain fatty acids arising from xylose fermentation by caecal microflora were measured using gas chromatography. The fatty acid profile produced by rock mouse gut microflora is similar to that for other animals that rely on gut microbial fermentation to produce fatty acids then used in oxidative metabolism. Chapter Five concludes with a discussion of possible explanations for the presence of xylose as a nectar sugar, its ecological significance, and the relevance of the fermentative capacity of pollinator digestive systems for xylose utilization in animals. / Thesis (PhD (Zoology))--University of Pretoria, 2007. / Zoology and Entomology / unrestricted

Ph.D., Faculty of Science (Botany), University of the Witwatersrand, 1990

Elephants (Loxodonta africana, Blumenbach) are recognised as keystone herbivores and ecosystem engineers in African ecosystems due to the noticeable impact they have on plant communities. This impact can influence other animal taxa and ecosystem processes, especially within enclosed systems. I investigated the effects of elephants in four vegetation types and the cascade effect on three associated taxa and two ecosystem processes at five sites with elephants and five paired sites without elephants in the Eastern Cape Province, South Africa, between April 2005 and July 2007. In grassland habitats, the physical structure of the vegetation was neither enhanced nor degraded in the presence of elephants. Within the locally important Thicket Biome, elephant browsing transformed relatively homogeneous stands of vegetation into more heterogeneous units. However, although seeds from thicket plant species were recorded in elephant dung, elephants did not promote the viability and germination success of these plants. In addition, elephant foraging on aloes caused declines in their populations. By contrast, the size of bushclumps was not reduced in the presence of elephants in bushclump savanna. The associated insect, bird and mammal communities appeared to benefit from elephant foraging in all vegetation types assessed, whereas vegetation patch dynamics and soil surface processes were neither enhanced nor degraded in the presence of elephants. I conclude that, at current densities, elephants do not (in most cases) negatively affect plant and animal communities or ecosystem processes in enclosed reserves. However, elephants have only been present at each site for a relatively short period and it is likely that their impact will be cumulative, increasing over time in these closed systems. Thus, future research along a continuum of elephant density and time since re-introduction is recommended.

Herbivores have a significant influence on their environment. Factors that influence herbivore distribution in a landscape are important for conservation. Artificial water provision is one such factor, with water sources being focal points of herbivore activity. Variation between herbivore utilisation of different waterhole types and habitat integrity surrounding the different waterholes is assessed in this study. Correlations are drawn between herbivore utilisation and habitat integrity to quantify the impact that artificial waterholes have on the landscape. A scoring system is devised to investigate the various factors affecting vegetation around artificial waterholes. Results show that there are significant variations between herbivore utilisation for different artificial waterhole types, as well as significant variation in habitat integrity surrounding the different waterhole types. Distance between waterholes and drainage lines, and utilisation by specific herbivore species have a significant impact on habitat integrity - specifically affecting veld condition and disturbance observed on woody plant species. A habitat score that was created by combining data from both the herbaceous and woody layers is not affected by waterhole type, distance from waterholes, or the different herbivore species utilising the different waterhole types. Earth dams have the greatest impact on surrounding vegetation and are the most utilised waterhole type. Closures of earth dams are not recommended due to their importance to herbivores. Less utilised waterhole types are also important, mitigating the impact of herbivore damage to vegetation at earth dams. / Environmental Sciences / M. Sc. (Nature Conservation)

South Africa’s growing elephant populations are concentrated in relatively small enclosed protected areas resulting in the over utilisation of the available food sources. Elephants and other herbivores as well as other natural disturbances such as fires and droughts play an important role in maintaining savannah environments. When these disturbances become too concentrated in a particular area the vegetation composition may be negatively affected. Excessive damage to the vegetation would result from exceeding the capacity of a protected area to provide food resources. The effect of the 120 elephants on the vegetation of Welgevonden Private Game Reserve, is not known. The rugged terrain of this reserve makes it a difficult, time consuming and labour intensive exercise to conduct ground studies. Satellite images can be used as a monitoring tool for vegetation change and improve the quantity and quality of environmental data to be collected significantly, allowing more informed management decision-making. This study evaluated the use of satellite imagery for monitoring elephant induced vegetation change on Welgevonden Private Game Reserve. The LANDSAT Thematic Mapper multispectral images, acquired at two yearly intervals from 1993 until 2007 were used. However, no suitable images were available for the years 1997, 2001 and 2003. A series of vegetation change maps was produced and the distribution of water sources and fire occurrences mapped. The areas of change were then correlated with the spatial distribution of water points and fire occurances, with uncorrelated areas of change. This was analysed using large animal population trends, weather data and management practices. On the visual comparison of the vegetation maps, it was seen that over this time period there was some decrease and thinning of woodland, but the most notable change was the increase of open woodland and decrease in grasslands. Using only the digital change detection for the period 1993 to 2007, a general increase in vegetation cover is seen. But this generalisation is misleading, since comparing the digital change detection to the vegetation maps indicates that while vegetation cover may have increased, significant changes occurred in the vegetation types. Most of the areas of significant change that were identified showed a strong positive correlation with burnt areas. The distribution of the water sources could not be directly linked to the vegetation change although rainfall fluctuations seemed to have accelerated vegetation changes. Years with high game counts, such as 1999, also coincide with very low rainfall making it difficult to differentiate between the effects of heavy utilisation of vegetation and low rainfall. Furthermore, many of the initial vegetation changes could be the result of land use changes due to the introduction of browsers, selective grazers and elephants that allow for more natural utilisation of the vegetation. Remote sensing makes it possible to successfully track changes in vegetation and identify areas of potential elephant induced vegetation change. Vegetation changes caused by disturbances, such as fire and anthropogenic activities, can be accounted for but it is not possible to conclude with a high level of certainty that the further changes seen are solely a result of elephant damage. Further work is required to reliably isolate elephant induced vegetation changes, as well as to establish the effects these changes have on the ecosystem as a whole. / Environmental Sciences / (M. Sc. (Environmetal Sciences))

South Africa’s growing elephant populations are concentrated in relatively small enclosed protected areas resulting in the over utilisation of the available food sources. Elephants and other herbivores as well as other natural disturbances such as fires and droughts play an important role in maintaining savannah environments. When these disturbances become too concentrated in a particular area the vegetation composition may be negatively affected. Excessive damage to the vegetation would result from exceeding the capacity of a protected area to provide food resources. The effect of the 120 elephants on the vegetation of Welgevonden Private Game Reserve, is not known. The rugged terrain of this reserve makes it a difficult, time consuming and labour intensive exercise to conduct ground studies. Satellite images can be used as a monitoring tool for vegetation change and improve the quantity and quality of environmental data to be collected significantly, allowing more informed management decision-making. This study evaluated the use of satellite imagery for monitoring elephant induced vegetation change on Welgevonden Private Game Reserve. The LANDSAT Thematic Mapper multispectral images, acquired at two yearly intervals from 1993 until 2007 were used. However, no suitable images were available for the years 1997, 2001 and 2003. A series of vegetation change maps was produced and the distribution of water sources and fire occurrences mapped. The areas of change were then correlated with the spatial distribution of water points and fire occurances, with uncorrelated areas of change. This was analysed using large animal population trends, weather data and management practices. On the visual comparison of the vegetation maps, it was seen that over this time period there was some decrease and thinning of woodland, but the most notable change was the increase of open woodland and decrease in grasslands. Using only the digital change detection for the period 1993 to 2007, a general increase in vegetation cover is seen. But this generalisation is misleading, since comparing the digital change detection to the vegetation maps indicates that while vegetation cover may have increased, significant changes occurred in the vegetation types. Most of the areas of significant change that were identified showed a strong positive correlation with burnt areas. The distribution of the water sources could not be directly linked to the vegetation change although rainfall fluctuations seemed to have accelerated vegetation changes. Years with high game counts, such as 1999, also coincide with very low rainfall making it difficult to differentiate between the effects of heavy utilisation of vegetation and low rainfall. Furthermore, many of the initial vegetation changes could be the result of land use changes due to the introduction of browsers, selective grazers and elephants that allow for more natural utilisation of the vegetation. Remote sensing makes it possible to successfully track changes in vegetation and identify areas of potential elephant induced vegetation change. Vegetation changes caused by disturbances, such as fire and anthropogenic activities, can be accounted for but it is not possible to conclude with a high level of certainty that the further changes seen are solely a result of elephant damage. Further work is required to reliably isolate elephant induced vegetation changes, as well as to establish the effects these changes have on the ecosystem as a whole. / Environmental Sciences / (M. Sc. (Environmetal Sciences))