Made available in DSpace on 2016-04-28T20:18:35Z (GMT). No. of bitstreams: 1 Maria Clara da Silva Negreiros Botelho.pdf: 723451 bytes, checksum: 1e37e85e4e70439cfb2171e80f21bc04 (MD5) Previous issue date: 2010-03-08 / The canine monocytic ehrlichiosis, an infectious disease of worldwide distribution, is caused by the rickettsia Ehrlichia canis, an obligatory intracellular parasite of mononuclear cells and transmitted by the dog tick Rhipicephalus sanguineus. The clinical and hematological abnormalities are varied, depending on the strain of Ehrlichia spp., the immune response of the dog and the concomitant presence of other diseases. Among the various hematological disorders, thrombocytopenia is a common feature. The disease can manifest itself through a variety of clinical veterinarians, particularly those working in endemic areas, should always consider the possibility of infection with Ehrlichia spp., especially in dogs with nonspecific signs of illness. This study aimed to evaluate clinical and hematologic recuperation of dogs naturally infected with Ehrlichia sp. and treated with doxycycline. The trial was performided at the Laboratory of Experimental Chemotherapy from the Department of Animal Parasitology of the Institute of Veterinary of the Universidade Federal Rural do Rio de Janeiro. About the total of 90 Beagles dogs kept in kennels experimental laboratory, twelve were selected, with clinical signs such as fever, anorexia, vomiting, weight loss, pale mucous membranes, hepatomegaly and splenomegaly and the presence of ticks R. sanguineus and find hematologic thrombocytopenia. The laboratory diagnosis was confirmed by direct identification of morulae of Ehrlichia sp. in blood smears stained with Giemsa. After weighing all of 12 dogs, they were individually treated with doxycycline at a dose of 10mg/kg body weight every 12 hours for 21 days, during which assessments were made of clinical and hematologic exams. Some clinical changes were observed during the evaluation period as fever, anorexia, vomiting, diarrhea, pale mucous membranes, splenomegaly and lymphadenopathy presenting these standard parameters the 21 days of treatment. The abnormalities found were thrombocytopenia and normochromic normocitic anemia. In the days +14 and +21, the mean counts of red blood cells and hematocrit value were within normal physiological parameters for the species. There was a statistically significant difference between days -1 and +21 by red blood counts and hematocrit values. At the end of treatment, all animals were normalized to platelet count, statistically significant differences between days -1 and +21. It is concluded that there was clinical-hematological normalization at the end of the treatment period and that it administered at a dose of 10mg/kg over 21 days produced no clinical and hematological reactions. Key words: Antibioticotherapy, Ehrlichia spp., Hematology. / A ehrlichiose monoc?tica canina, doen?a infecciosa de distribui??o mundial, ? causada pela riqu?tsia Ehrlichia canis, parasita intracelular obrigat?rio de c?lulas mononucleares caninas e transmitida pelo carrapato Rhipicephalus sanguineus. As anormalidades cl?nicas e hematol?gicas s?o variadas, dependendo da linhagem de Ehrlichia spp., da resposta imune do c?o e da presen?a concomitante de outras doen?as. Entre as diversas anormalidades hematol?gicas, a trombocitopenia ? um achado predominante. Como a doen?a pode se manifestar atrav?s de uma grande variedade de sinais cl?nicos, os m?dicos veterin?rios, particularmente aqueles que trabalham em regi?es end?micas, devem sempre considerar a possibilidade de infec??o por Ehrlichia spp., especialmente em c?es com sinais n?o espec?ficos da doen?a. O presente estudo teve como objetivo avaliar a recupera??o cl?nicohematol?gica de c?es naturalmente infectados por Ehrlichia sp. e tratados com doxiciclina. O estudo foi realizado nas depend?ncias do Laborat?rio de Quimioterapia Experimental em Parasitologia Veterin?ria do Departamento de Parasitologia Animal da UFRRJ. Selecionou-se 12 animais da ra?a Beagle, do total de 90 c?es mantidos no canil experimental do laborat?rio, com sinais cl?nicos, como febre, anorexia, v?mito, emagrecimento, mucosas hipocoradas, hepato e esplenomegalia, al?m da presen?a de carrapatos R. sanguineus e do achado hematol?gico de trombocitopenia. O diagn?stico laboratorial foi confirmado pela identifica??o direta de m?rulas de Ehrlichia sp. em esfrega?os sangu?neos corados por Giemsa. Ap?s a pesagem dos 12 c?es, os mesmos foram tratados individualmente com doxiciclina, na dose de 10mg/kg de peso corporal, por via oral, a cada 12 horas por um per?odo de 21 dias, durante os quais foram realizadas avalia??es de par?metros cl?nicos e hematol?gicos. Algumas altera??es cl?nicas foram observadas ao longo do per?odo de avalia??o como febre, anorexia, v?mito, diarr?ia, mucosas hipocoradas, esplenomegalia e linfadenomegalia apresentando-se estes par?metros normalizados ao 21? dia de tratamento. As altera??es hematol?gicas encontradas foram trombocitopenia e anemia normocit?ca normocr?mica. Nos dias +14 e +21, as contagens m?dias de hem?cias e valor de hemat?crito estavam dentro dos par?metros fisiol?gicos normais para a esp?cie. Houve diferen?a estat?stica significativa entre os dias -1 e +21 na contagem de hem?cias e nos valores de hemat?crito. Quanto a trombocitopenia, no fim do tratamento, todos animais estavam com plaquetometria normalizada, havendo diferen?a estat?stica significativa entre os dias -1 e +21. Conclui-se que houve uma normaliza??o dos quadros cl?nico-hematol?gicos ao final do per?odo de tratamento e que o mesmo administrado na dose de 10mg/Kg ao longo de 21 dias n?o produziu rea??es cl?nicas e hematol?gicas adversas. Palavras-chave: Antibioticoterapia, Ehrlichia spp., Hematologia.

In our study we analyzed pathogenic microorganisms from burn wounds, their rate and changes after tightening the infection control. Special attention was paid to the diagnostics and treatment problems of Staphylococcus aureus. We have been studying Rational application algorithm of antibacterial drugs influence on the effectiveness of the infection diagnostics and treatment, and the changes of Staphylococcus aureus resistance to antibiotics. There was performed an experimental analysis of pathogenic microorganisms with „electronic nose“ and a comparison was made of changing parameters in „electronic nose“ resistance studying Acinetobacter spp., Escherichia coli, Pseudomonas aeruginosa, Staphylococus aureus pathogenic microorganisms. This experiment underlies further investigations of the early diagnostics of the pathogenic microorganisms. Our research works have characterized it from the complex standpoint to the solution of burn wound infection prevention, diagnostics, and treatment problems what is missing in analyzed literature.