Thesis (PhD) -- AUT University, 2008. / Includes bibliographical references. Also held in print (285 leaves : ill. ; 30 cm.) in the Archive at the City Campus (T 338.3718 JOH)

Thèses (M.Env.)--Université de Sherbrooke (Canada), 2005. / Titre de l'écran-titre (visionné le 20 juin 2006). Publié aussi en version papier.

Thèses (M.Sc.)--Université de Sherbrooke (Canada), 2005. / Titre de l'écran-titre (visionné le 20 juin 2006). Publié aussi en version papier.

Th. doct.--Agronomie--Paris-Grignon--Institut natl.agronomique, 1990. / Résumé en anglais. Bibliogr. p. 273-293.

Thesis (M.S.)--University of Wisconsin--Madison, 1984. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 127-131).

Thesis (M.S.)--University of Wisconsin--Madison, 1982. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 117-118).

Thesis (Ph.D.) - University of Queensland, 2003. / Includes bibliography.

Th. 3e cycle--Sci. et tech. en prod. animale--Toulouse--I.N.P., 1981. N°: 91.

Resumo: Objetivou-se com o presente estudo analisar a viabilidade de gerenciamento do MAVIPI (Modelo Alto Vale do Itajaí de Piscicultura Integrada) usando-se um banco de dados integrado, estruturado com base nas informações sistematicamente coletadas e armazenadas durante 10 anos de condução do projeto. Inicialmente são apresentados os fatores que viabilizaram a proposição do MAVIPI. Na sequencia foi apresentada a base teórica que da suporte ao MAVIPI e que foi utilizada referencia para a avaliações conduzidas usando-se os indicadores desenvolvidos. Finalmente foram apresentadas propostas de alguns indicadores e ferramentas de análise produzida a partir do banco de dados e possíveis interpretações. Mas um grande número deles pode ser obtido. Como conclusão fica a constatação que um banco de dados como que foi compilado, a partir de anotações baseadas em algumas ferramentas disponíveis, permite o desenvolvimento de um número muito grande de indicadores e ferramentas de gerenciamento. Entretanto a definição de quais indicadores são os mais adequados, deve ser objeto de discussão com o publico alvo. Mas considerando que o publico alvo do MAVIPI são os pequenos piscicultores, sem muito conhecimento teórico sobre gerenciamento, a participação de extensionistas públicos é fator determinante / Abstract: The objective of this study was to determine the feasibility of managing MAVIPI (Modelo Alto Vale do Itajai of Integrated Fish Farming) as a unique enterprise, using a database assembled from information that was collected and stored for 10 years driving the project aimed to manage individual production cycles. Initially was presented the factors that enabled the proposition MAVIPI. At next step was discussed theoretical basis supporting MAVIPI and will be used as a reference for evaluations conducted using the indicators developed. Finally there were proposals of some indicators and assessment tools generated from the database and possible interpretations. However, a much large number of them can be easily developed. Conclusion is the finding that a database compiled from notes and also from some tools available, allows the development of a very large number of indicators and management tools. However, the definition of which ones are most appropriate, should be result of a deeper discussed with the audience. But considering the target audience of MAVIPI are small farmers without much theoretical knowledge about management, the participation agents of community extension appears to be a determining factor / Orientador: Maria Inez Espagnoli Geraldo Martins / Coorientador: Gener Tadeu Pereira / Banca: Newton José Rodrigues da Silva / Banca: Daniel Yokoyama Sonoda / Banca: João Batista Kochenborger Fernandes / Banca: João Donato Scorvo Filho / Doutor

The pirarucu (Arapaima gigas) from Amazon basin is currently the largest farmed fish species and its production is increasing rapidly in Brazil. However, there is a concern about bacterial disease outbreaks and resulting mortalities in pirarucu farms. The use of probiotic bacteria as prophylactic method is recognized as beneficial practice to enhance fish production. The aim of this thesis was to isolate autochthonous probiotic strains from the pirarucu intestine, characterize their potential probiotic characteristics in vitro, and perform in vivo colonization and growth experiments. To this end, the intestinal microbial community of A. gigas was assessed at two different growth stages using high-throughput sequencing (HTS) analysis. Proteobacteria, Fusobacteria and Firmicutes were the most abundant phyla. At genus level Bradyrhizobium and Cetobacterium were the most abundant in adult and juvenile fish, respectively. In a further trial two isolated lactic acid bacteria (LAB): Lactococcus lactis subsp. lactis and Enterococcus faecium, showed in vitro suitability as probiotics. Two potential pathogens: Citrobacter freundii and Pseudomonas sp., were also isolated and their pathogenicity assessed. Subsequently, an in vivo experiment was performed to assess the potencial of LAB strains to colonise and modulate the gut microbiome of pirarucu after 21 days of feeding. The results showed a high abundance of Cetobacterium in all treatments. Additionally, both probiotic treatments decreased the levels of Clostridiales in pirarucu intestine and showed adherence to the fish mucosal tissue. Finally, a growth experiment was performed to assess the ability of to the candidate probiotics to improve growth parameters after 42 days of feeding. HTS confirmed that Cetobacterium was the most abundant genus in all treatments. Fish fed with L. lactis subsp. lactis presented higher percentage of increase (%I) of weight, specific growth rate (SGR), and monocytes in blood. The strain E. faecium interacted with the microbial gut community and was able to populate the mucosal tissue. In conclusion, both LAB strains presented probiotic characteristics and should be considered as probiotics in A. gigas farming. These probiotics could contribute to a reduction in antibiotics use in pirarucu farms, thus, adding value to the species as a sustainable aquaculture product.