An experimental study was made of accumulation and loss of heavy metals by the aquatic moss Rhynchostegium riparioides and of the processes involved. The information gained were used to assess the effectiveness of this species as a monitor. Growth of Rhynchostegium continued throughout the year with peaks in spring and autumn. There were positive correlations between growth and water and air temperatures. Strong differences in growth rates in the four streams were not related to nutrient or heavy metal concentrations. Various patterns of mesh bag were tested as containers for transplanted Rhynchostegium to be used as a monitor. No significant differences in accumulation by moss were found between boulders or bags, or in accumulation with different patterns of bags. Accumulation was reduced slightly at the centre of bags packed with large quantities of moss. The physiology of Zn accumulation was also studied. A large part of accumulation (> 70%) in the early stages (< 12 h) was in a form readily exchanged for competing cations such as Ca and Ni; over longer time periods there was significant accumulation into a more tightly bound compartment. There was no evidence that uptake into this latter compartment was under the direct control of the plant's metabolism. There was differential accumulation of Cr(III) and Cr(VI) in laboratory experiments; during a case study it was possible to "predict" the speciation of Cr in the water by the concentrations accumulated by the moss. These results confirm that bryophytes are useful as monitors of heavy metal pollution in a wide range of circumstances. A range of such applications are outlined, along with recommendations for standard methods for using moss bags.

This diploma thesis is focused on determination of bioavailable cadmium in natural waters. Thesis is devided to two parts-laboratory study in model solution and experiments in real surface water system. During the laboratory experiments the accumulation and release constants of cadmium by aquatic moss Fontinalis antipyretica were determined. In Morava river experiment this aquatic moss was used to determine bioavailable fraction of cadmium and the moss bag technique were compared with another methods for phytotoxic metals determination.

Use of arsenic-rich groundwater for crop irrigation can increase the arsenic (As) content in food crops and act as a carcinogen, compromising human health. Using aquatic plant based phytofiltration is a potential eco-technique for removing arsenic from water. The aquatic moss species Warnstorfia fluitans grows naturally in mining areas in northern Sweden, where high concentrations of arsenic occur in lakes and rivers. This species was selected as a model for field, climate chamber and greenhouse studies on factors governing arsenic removal and arsenic phytofiltration of irrigation water. The arsenic and silicon (Si) concentrations in soil, water and plant samples were measured by AAS (atomic absorption spectrophotometry), while arsenite and arsenate species were determined using AAS combined with high pressure liquid chromatography (HPLC) with an anion exchange column. The arsenic content in grains of hybrid and local aromatic rice (Oryza sativa) cultivars with differing arsenic accumulation factor (AF) values was investigated in an arsenic hotspot in Bangladesh. The results showed that arsenic AF was important in identifying arsenic-safer rice cultivars for growing in an arsenic hotspot. The study based on silicon effect on arsenic uptake in lettuce showed that arsenic accumulation in lettuce (Lactuca sativa) could be reduced by silicon addition. The aquatic moss had good phytofiltration capacity, with fast arsenic removal of up to 82% from a medium with low arsenic concentration (1 µM). Extraction analysis showed that inorganic arsenic species were firmly bound inside moss tissue. Absorption of arsenic was relatively higher than adsorption in the moss. Regarding effects of different abiotic factors, plants were stressed at low pH (pH 2.5) and arsenic removal rate was lower from the medium, while arsenic efflux occurred in arsenate-treated medium at low (12°C) and high (30°C) temperature regimes. Besides these factors, low oxygenation increased the efficiency of arsenic removal from the medium. Finally, combining W. fluitans as a phytofilter with a lettuce crop on a constructed wetland significantly reduced the arsenic content in edible parts (leaves) of lettuce. Thus W. fluitans has great potential for use as an arsenic phytofilter in temperate regions. / <p>QC 20170323</p>