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The Global ETD Search service is a free service for researchers
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From eye lens cells to lens membrane proteins : Development and application of a hybrid high-speed atomic force microscopy/optical microscopy setup / From eye lens cells to lens membrane proteins : Development and application of a hybrid high-speed atomic force microscopy/optical microscopy setupColom diego, Adai 11 July 2013 (has links)
Je utilise le AFM et le HS-AFM pour étudier les caractéristiques mécaniques du cellule du cristallin et aussi des protéines de membrane de la cellule, AQP0 et Connexon. L’énergie d'interaction de la AQP0 est -2.7 kBT, très nécessaire pour former les microdomaines de jonctions (junctional microdomain). Aussi c' est la première fois qu il est possible de voir des protéines individuel et son mouvement en cellules vivants. La formation de microdomaines est important pour la transparence du cristallin, et le AQP1 ne le peux faire. / I used the AFM and HS-AFM for characterise the eye lens and the eye lens membrane protein, AQP0 and connexon.A QP0-AQP0 interaction energy is -2.7kBT, it is important for the formation of junctional microdomains, which keep the distance between the cells lens and lens transparency. this is the first report which is present time the visualization of unlabelled membrane proteins on living cells under physiological conditions. AQP1 can not maintain the lens transparency because it does not form junctional microdomains.
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