Lactogenesis II is critical stage of lactation and it is hindered by conditions like caesarean section, premature delivery, obesity, age, and parity. Previous studies have shown a connection between morphological characteristics of breast and nipple-areola complex and lactation onset. Higher number of areolar glands and their non-uniform distribution has been linked to earlier onset of lactation. Extremely large breasts and short nipples has been linked to breastfeeding difficulty. Aim of this study was to investigate relationship between morphological characteristics of breast and nipple-areola complex (number and distribution of areolar glands, nipple, areola, and breast area, protrusion of nipple and areola, and contrast of breast and areola) and lactation onset. For this purpose, we collected breasts and areolae photographs of 141 mothers one day postpartum in maternity ward in Prague and compared the morphological traits to lactogenesis II onset. We have found a non-uniform distribution of areolar glands with the highest abundance on upper lateral quadrant of areola. We found that right breast area tends to be bigger than left, and left nipple and areola tends to be longer than right. Parity had significant effect on lactation onset. No morphological characteristics have been linked to...