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1	Développement de méthodes d'assemblage de génomes de novo adaptées aux bactéries endosymbiotes Théroux, Jean-François 04 1900 (has links) Le but de ce projet était de développer des méthodes d'assemblage de novo dans le but d'assembler de petits génomes, principalement bactériens, à partir de données de séquençage de nouvelle-génération. Éventuellement, ces méthodes pourraient être appliquées à l'assemblage du génome de StachEndo, une Alpha-Protéobactérie inconnue endosymbiote de l'amibe Stachyamoeba lipophora. Suite à plusieurs analyses préliminaires, il fut observé que l’utilisation de lectures Illumina avec des assembleurs par graphe DeBruijn produisait les meilleurs résultats. Ces expériences ont également montré que les contigs produits à partir de différentes tailles de k-mères étaient complémentaires pour la finition des génomes. L’ajout de longues paires de lectures chevauchantes se montra essentiel pour la finition complète des grandes répétitions génomiques. Ces méthodes permirent d'assembler le génome de StachEndo (1,7 Mb). L'annotation de ce génome permis de montrer que StachEndo possède plusieurs caractéristiques inhabituelles chez les endosymbiotes. StachEndo constitue une espèce d'intérêt pour l'étude du développement endosymbiotique. / The goal of this project was to develop de novo genome assembly methods adapted to small genomes, especially bacterial, using next-generation sequencing data. Eventually, these methods could be used to assemble the genome of StachEndo, an unknown Alpha-Proteobacteria ensymbiont of the Stachyamoeba lipophora amoeba. Preliminary findings showed that the use of Illumina reads with DeBruijn graph assemblers yielded the best results. These experiments also showed that contigs produced with k-mers of various sizes were complementary in genome finishing assays. The addition of long-range paired-end reads proved necessary to fully close genomic assembly gaps. These methods made the assembly of StachEndo’s genome (1.7 Mb) possible. Through the annotation of StachEndo’s genes, several features that are unusal for endosymbionts were identified. StachEndo seems to be an interesting species for the study of endosymbiotic evolution. assemblage de génome de novo séquençage nouvelle-génération qualité d'assemblage graphe DeBruijn k-mère endosymbiote Rickettsiales de novo genome assembly next-generation sequencing assembly quality DeBruijn graph k-mer endosymbiont Rickettsiales
2	Development and Evaluation of a Machine Vision System for Digital Thread Data Traceability in a Manufacturing Assembly Environment Alexander W Meredith (15305698) 29 April 2023 (has links) <p>A thesis study investigating the development and evaluation of a computer vision (CV) system for a manufacturing assembly task is reported. The CV inference results are compared to a Manufacturing Process Plan and an automation method completes a buyoff in the software, Solumina. Research questions were created and three hypotheses were tested. A literature review was conducted recognizing little consensus of Industry 4.0 technology adoption in manufacturing industries. Furthermore, the literature review uncovered the need for additional research within the topic of CV. Specifically, literature points towards more research regarding the cognitive capabilities of CV in manufacturing. A CV system was developed and evaluated to test for 90% or greater confidence in part detection. A CV dataset was developed and the system was trained and validated with it. Dataset contextualization was leveraged and evaluated, as per literature. A CV system was trained from custom datasets, containing six classes of part. The pre-contextualization dataset and post-contextualization dataset was compared by a Two-Sample T-Test and statistical significance was noted for three classes. A python script was developed to compare as-assembled locations with as-defined positions of components, per the Manufacturing Process Plan. A comparison of yields test for CV-based True Positives (TPs) and human-based TPs was conducted with the system operating at a 2σ level. An automation method utilizing Microsoft Power Automate was developed to complete the cognitive functionality of the CV system testing, by completing a buyoff in the software, Solumina, if CV-based TPs were equal to or greater than human-based TPs.</p> Automation engineering Flexible manufacturing systems Industrial engineering Manufacturing safety and quality Knowledge representation and reasoning Computer vision Object detection and classification object detection and tracking Smart Manufacturing Systems MES system Smart Manufacturing Approach Quality engineering Manufacturing automation systems manufacturing automation Cognitive Systems Engineering cognitive system computer vision machine vision manufacturing execution system robotic process automation Digital Thread (DT) Digital Thread digital twin approach manufacturing assembly assembly quality manufacturing traceability advanced manufacturing advanced quality control Quality 4.0 Industry 4.0 Industry 5.0 Quality 5.0 Human Machine Interactions

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Développement de méthodes d'assemblage de génomes de novo adaptées aux bactéries endosymbiotes

Development and Evaluation of a Machine Vision System for Digital Thread Data Traceability in a Manufacturing Assembly Environment