No description available.

Postcranial non-metric skeletal traits are documented in two Cis-Baikal populations: the Kitoi, dating to the Early Neolithic (8000-7000/6800 BP), and the Isakovo/Serovo/Glazkovo (ISG) cultural complex, dating to the Late Neolithic (Isakavo/Serovo: 6000/5800-5200 BP) and Early Bronze Age (Glazkovo: 5200/5000-4000 BP). A major cultural discontinuity is thought to have occurred in the Middle Neolithic (7000/6800-6000/5800 BP). Current and previous research suggests that the Early Neolithic Kitoi were bioculturally distinct from the Late Neolithic-Bronze Age ISG cultural complex. Population, side, sex, and age differences in the expression of non-metric traits were explored as indicators of differing activity patterns and divisions of labour between and within the Kitoi and ISG samples, as well as two Kitoi cemeteries: Shamanka II and Lokomotiv. Results of analyses indicate that the Kitoi, particularly males, were taking part in locomotion over steep terrain while carrying heavy loads, probably related to hunting trips. The ISG do not show evidence of these types of activities, suggesting a population difference in the frequency and degree of physically strenuous activity. Kitoi and Shamanka II males and young adult individuals show evidence of having performed the majority of the strenuous lifting and carrying, as well as evidence of increased mobility relative to females. These results indicative of strong divisions of labour in the Kitoi population. Patterns in trait frequencies were also examined for indications of how multiple underlying factors may be interacting. Trait distribution throughout the body provides evidence of the dominance of biomechanical stress as a causative factor in the expression of postcranial non-metric traits. Other factors that become visible when the influence of biomechanical stress is lower include genotype, trauma, and cartilage degeneration. The results of this project are consistent with current theories on the adaptive regimes of the Kitoi and ISG populations and strongly support previous work by BAP researchers in the areas of skeletal robusticity, osteoarthritis, and musculoskeletal stress markers. The research also helps to broaden the knowledge base about the etiologies of the non-metric traits involved.

Postcranial non-metric skeletal traits are documented in two Cis-Baikal populations: the Kitoi, dating to the Early Neolithic (8000-7000/6800 BP), and the Isakovo/Serovo/Glazkovo (ISG) cultural complex, dating to the Late Neolithic (Isakavo/Serovo: 6000/5800-5200 BP) and Early Bronze Age (Glazkovo: 5200/5000-4000 BP). A major cultural discontinuity is thought to have occurred in the Middle Neolithic (7000/6800-6000/5800 BP). Current and previous research suggests that the Early Neolithic Kitoi were bioculturally distinct from the Late Neolithic-Bronze Age ISG cultural complex. Population, side, sex, and age differences in the expression of non-metric traits were explored as indicators of differing activity patterns and divisions of labour between and within the Kitoi and ISG samples, as well as two Kitoi cemeteries: Shamanka II and Lokomotiv. Results of analyses indicate that the Kitoi, particularly males, were taking part in locomotion over steep terrain while carrying heavy loads, probably related to hunting trips. The ISG do not show evidence of these types of activities, suggesting a population difference in the frequency and degree of physically strenuous activity. Kitoi and Shamanka II males and young adult individuals show evidence of having performed the majority of the strenuous lifting and carrying, as well as evidence of increased mobility relative to females. These results indicative of strong divisions of labour in the Kitoi population. Patterns in trait frequencies were also examined for indications of how multiple underlying factors may be interacting. Trait distribution throughout the body provides evidence of the dominance of biomechanical stress as a causative factor in the expression of postcranial non-metric traits. Other factors that become visible when the influence of biomechanical stress is lower include genotype, trauma, and cartilage degeneration. The results of this project are consistent with current theories on the adaptive regimes of the Kitoi and ISG populations and strongly support previous work by BAP researchers in the areas of skeletal robusticity, osteoarthritis, and musculoskeletal stress markers. The research also helps to broaden the knowledge base about the etiologies of the non-metric traits involved.

The goal of this thesis is to analyze the business environment in the Baikal region for the hospitality management industry, specifically for a 3-4 star hotel. The topic is very current and under discussion now because the Russian state authorities would like to create a place attractive for tourism in Baikal and there is currently an economically favorable environment for the hotel industry. There is also government support in promotion of the region for tourism and cooperation with other government or non-government association or organization for development tourism there.

No description available.

Few genomic and metagenomic studies have focused on freshwater systems in the last years. Most of the studies carried out on these particular environments so far rely on microscopy, physiology, phenotypic observations, individual genes and 16S rRNA sequencing. Here, we shed light on microbial communities from oligotrophic and mesotrophic freshwater systems using high-throughput deep sequencing metagenomics and genome-resolved metagenomics. We have focused on the study of ubiquitous and cosmopolitan microbial groups from two temperate Spanish reservoirs (Tous, Amadorio). Among these, we studied freshwater picocyanobacteria from Synechococcus and Cyanobium genera, which so far have not been well characterized at the genomic level, compared to the marine representatives. In particular, we were able to isolate two of the most abundant picocyanobacteria from Tous reservoir, which were previously studied via metagenomics. These picocyanobacteria are not only abundant in this reservoir but are widely distributed in different freshwater and brackish systems. In this work we also shed light on some of the first freshwater representatives of the phylum Verrucomicrobia, that are ecologically uncharacterized in freshwater systems about which relatively little is known. We discovered a wide range of metabolisms in these microbes, ranging from nitrogen fixation and photoheterotrophy via rhodopsin pumps to important contributions in the degradation of recalcitrant matter and polysaccharides. We also include the first metagenomic study of the microbial communities under the ice waters of the largest (by volume) ultraoligotrophic lake in the world, Lake Baikal. This study has provided a first glimpse and a particular microbial composition on the sub-ice, having found an unusual fraction of Verrucomicrobia and new microbial lineages from many typical freshwater phyla, including the first freshwater representative of the groups I/II of SAR11 lineage and novel genomes of Proteobacteria, Thaumarchaeaota, Gemmatimonadetes, Cyanobacteria, Planctomycetes, Bacteroidetes, Acidobacteria, Nitrospirae, Verrucomicrobia or Actinobacteria.

Local adaptation and phenotypic plasticity are main mechanisms of organisms’ resilience in changing environments. Both are affected by gene flow and are expected to be weak in zooplankton populations inhabiting large continuous water bodies and strongly affected by currents. Lake Baikal, the deepest and one of the coldest lakes on Earth, experienced epilimnion temperature increase during the last 100 years, exposing Baikal’s zooplankton to novel selective pressures. We obtained a partial transcriptome of Epischura baikalensis (Copepoda: Calanoida), the dominant component of Baikal’s zooplankton, and estimated SNP allele frequencies and transcript abundances in samples from regions of Baikal that differ in multiyear average surface temperatures. The strongest signal in both SNP and transcript abundance differentiation is the SW-NE gradient along the 600+ km long axis of the lake, suggesting isolation by distance. SNP differentiation is stronger for nonsynonymous than synonymous SNPs and is paralleled by differential survival during a laboratory exposure to increased temperature, indicating directional selection operating on the temperature gradient. Transcript abundance, generally collinear with the SNP differentiation, shows samples from the warmest, less deep location clustering together with the southernmost samples. Differential expression is more frequent among transcripts orthologous to candidate thermal response genes previously identified in model arthropods, including genes encoding cytoskeleton proteins, heat-shock proteins, proteases, enzymes of central energy metabolism, lipid and antioxidant pathways. We conclude that the pivotal endemic zooplankton species in Lake Baikal exists under temperature-mediated selection and possesses both genetic variation and plasticity to respond to novel temperature-related environmental pressures.

This thesis presents the analysis of environmental movements in Russia.Through a collective memory and discourse framework, this study reviews the overlap and disparity in perceptions of environmental movements in the Soviet Union and Russia.The portrait that emerges from the analysis of the environmental movements suggests that the impact of environmental movements in Russia may be limited in part because it has developed in contravention to existing discourses. More importantly, the context and underlying assumptions of environmental movements are not formulated in ways that are compatible with existing collective identities in Russian society.

Lake Baikal is the oldest, by volume, the largest, and the deepest freshwater lake on Earth. It is characterized by an outstanding diversity of endemic faunas with more than 350 amphipod species and subspecies (Amphipoda, Crustacea, Arthropoda). They are the dominant benthic organisms in the lake, contributing substantially to the overall biomass. Eulimnogammarus verrucosus, E. cyaneus, and E. vittatus, in particular, serve as emerging models in ecotoxicological studies. It was, then, necessary to investigate whether these endemic littoral amphipods species form genetically separate populations across Baikal, to scrutinize if the results obtained --~for example, about stress responses~-- with samples from one single location (Bolshie Koty, where the biological station is located), could be extrapolated to the complete lake or not. The genetic diversity within those three endemic littoral amphipod species was determined based on fragments of Cytochrome C Oxidase I (COI) and 18S rDNA (only for E. verrucosus). Gammarus lacustris, a Holarctic species living in water bodies near Baikal, was examined for comparison. The intra-specific genetic diversities within E. verrucosus and E. vittatus (13% and 10%, respectively) were similar to the inter-species differences, indicating the occurrence of cryptic, morphologically highly similar species. This was confirmed with 18S rDNA for E. verrucosus. The haplotypes of E. cyaneus and G. lacustris specimens were, with intra-specific genetic distances of 3% and 2%, respectively, more homogeneous, indicating no --or only recent disruption of-- gene flow of E. cyaneus across Baikal, and recent colonization of water bodies around Baikal by G. lacustris. The data provide the first clear evidence for the formation of cryptic (sub)species within endemic littoral amphipod species of Lake Baikal and mark the inflows/outflow of large rivers as dispersal barriers. Lake Baikal has provided a stable environment for millions of years, in stark contrast to small, transient water bodies in its immediate vicinity. A highly diverse endemic amphipod fauna is found in one but not the other habitat. To gain more insights and explain the immiscibility barrier between Lake Baikal and non-Baikal environments faunas, the differences in the stress response pathways were studied. To this end, exposure experiments to increasing temperature and a heavy metal (cadmium) as proteotoxic stressors were conducted in Russia. High-quality de novo transcriptome assemblies were obtained, covering multiple conditions, for three amphipod species: E. verrucosus and E. cyaneus -Baikal endemics-, and G. lacustris -Holarctic- as a potential invader. After comparing the transcriptomic stress responses, it was found that both Baikal species possess intact stress response systems and respond to elevated temperature with relatively similar changes in their expression profiles. G. lacustris reacts less strongly to the same stressors, possibly because its transcriptome is already perturbed by acclimation conditions (matching the Lake Baikal littoral). Comprehensive genomic resources are of utmost importance for ecotoxicological and ecophysiological studies in an evolutionary context, especially considering the exceptional value of Baikal as a UNESCO World Heritage Site. In that context, the results presented here, on the genome of Eulimnogammarus verrucosus, have been the first massive step to establish genomic sequence resources for a Baikalian amphipod (other than mitochondrial genomes and gene expression data in the form of de novo transcriptomes assemblies). Based on the data from a survey of its genome (a single lane of paired-end Illumina HiSeq 2000 reads, 3X) as well as a full dataset (two complete flow cells, 46X) the genome size was estimated as nearly 10 Gb based on the k-mer spectra and the coverage of highly conserved miRNA, hox genes, and other Sanger-sequenced genes. At least two-thirds of the genome are non-unique DNA, and no less than half of the genomic DNA is composed of just five families of repetitive elements, including low complexity sequences. Some of the repeats families found in high abundance in E. verrucosus seem to be species-specific, or Baikalian-specific. Attempts to use off-the-shelf assembly tools on the available low coverage data, both before and after the removal of highly repetitive components, as well as on the full dataset, resulted in extremely fragmented assemblies. Nevertheless, the analysis of coverage in Hox genes and their homeobox showed no clear evidence for paralogs, indicating that a genome duplication did not contribute to the large genome size. Several mate-pair libraries with bigger insert sizes than the 2kb used here and long reads sequencing technology combined with semi-automated methods for genome assembly seem to be necessary to obtain a reliable assembly for this species.

Lake Baikal, in Siberia, Russia, contains the highest biodiversity of any extant lake, including an impressive radiation of gammaroidean amphipods that are often cited as a classic case of adaptive radiation. However, relationships among Baikal's amphipods remain poorly understood. The phylogenetic history of 32 Lake Baikal amphipod species, representing most major lineages of the endemic fauna, was examined using three genes (COI, 16S rRNA, and 18S rRNA), and 152 morphological characters. Results support monophyly of the largest and most diverse of the Baikalian families, the Acanthogammaridae. Analyses suggest that a second Baikalian family, the fossorial Micruropodidae, is paraphyletic and composed of two divergent clades, one of which includes Macrohectopus branickii, a morphologically specialized pelagic planktivore traditionally assigned its own family. The extreme morphological and ecological divergence of Macrohectopus from its close genetic relatives, and conversely, the large genetic distances among other morphologically similar micruropodids, suggest that morphological and molecular evolution have often been uncoupled during the radiation of Baikal's amphipods. This study suggests that the amphipod fauna of Lake Baikal is polyphyletic; originating from two independent invasions of the lake.