Mountain pine beetle (Dendroctonus ponderosae, Hopkins) is a major disturbance agent in pine ecosystems of western North America. Adaptation to local climates has resulted in primarily univoltine (one generation per year) generation timing across a thermally diverse latitudinal gradient. We hypothesized that this pattern in total development time is shaped by selection for slower developmental rates, altered developmental thresholds, or oviposition rates in southern populations inhabiting warmer climates. To investigate traits responsible for latitudinal differences we measured lifestage-specific development of southern mountain pine beetle eggs, larvae and pupae across a range of temperatures. We also describe and model oviposition of southern US MPB. Using a novel technique that included frequent X-ray imaging, oviposition rate and fecundity were estimated separately and shown to both be significant and independent sources of variation. When compared with previously collected data for a northern MPB population, total oviposition time predicted for southern MPB at a constant 20°C was slightly longer than that of northern MPB, but the delay was too small to account for significant differences between the populations in total development time. Developmental rate curves for eggs, larvae, and pupae were fit using maximum posterior likelihood estimation with a Bayesian prior to improve fit stability. When compared to previously published data for a northern population (Régnière et al. 2012), observed developmental rates of the southern and northern populations were similar across all studied lifestages at 20 and 25°C, although southern individuals were generally faster at temperature extremes (10 and 27°C). These findings were inconsistent with our hypothesis that southern individuals would have consistently slower rates. Optimal development of southern individuals occurred at higher temperatures, with higher development thresholds, as compared with northern individuals. Our results suggest that evolved traits in the remaining unstudied lifestage, teneral (i.e., pre-emergent) adult, likely influence latitudinal differences in mountain pine beetle generation time.

<p>The objective for this master’s thesis was to test and evaluate two methods for determining the content of tannin in a pinebarkextract. The methods used at Södra for this previously have not been specific enough, only the amount of polyphenolics have been measured. One of these methods is a test based on the Stiasny test and it determines the extracts ability to form a gel with formaldehyde. When this test was carried out it showed that the extract did not contain much tannin. The other method used at Södra measures the amount of polyphenolic substances with a spectrophotometer. The result from this method showed that the content were 50 %.</p><p> </p><p>One of the two new methods that were evaluated determines the amount tannin present in the extract because of tannins ability to form a complex with proteins. According to this method, the amount tannin in the extract were 42,5 %, and 19,4 % of this in the form of tannic acid. These results are credible on basis of previous information about the extract.</p><p> </p><p>The other method uses a RP- HPLC where ellagic and gallic acid were used as standards. The amount ellagic acid in the extract was determined to 0,06 %, but the result from gallic acid was inconclusive. Further analysis is necessary to evaluate the obtained results and the methods reliability.</p><p> </p><p>If the metods is to be used on a regular basis, my recommendation would be to start evaluating the protein-binding method because this would be easier and less time-consuming than modifying the HPLC- method. The HPLC- method could on the other hand provide useful information about the extract, not only the amount tannin could be measured, but also which kind of tannin could be investigated.</p>

The objective for this master’s thesis was to test and evaluate two methods for determining the content of tannin in a pinebarkextract. The methods used at Södra for this previously have not been specific enough, only the amount of polyphenolics have been measured. One of these methods is a test based on the Stiasny test and it determines the extracts ability to form a gel with formaldehyde. When this test was carried out it showed that the extract did not contain much tannin. The other method used at Södra measures the amount of polyphenolic substances with a spectrophotometer. The result from this method showed that the content were 50 %.   One of the two new methods that were evaluated determines the amount tannin present in the extract because of tannins ability to form a complex with proteins. According to this method, the amount tannin in the extract were 42,5 %, and 19,4 % of this in the form of tannic acid. These results are credible on basis of previous information about the extract.   The other method uses a RP- HPLC where ellagic and gallic acid were used as standards. The amount ellagic acid in the extract was determined to 0,06 %, but the result from gallic acid was inconclusive. Further analysis is necessary to evaluate the obtained results and the methods reliability.   If the metods is to be used on a regular basis, my recommendation would be to start evaluating the protein-binding method because this would be easier and less time-consuming than modifying the HPLC- method. The HPLC- method could on the other hand provide useful information about the extract, not only the amount tannin could be measured, but also which kind of tannin could be investigated.

No description available.

Thesis (M.S.)--Oregon State University, 2010. / Printout. Includes bibliographical references (leaves 87-92). Also available on the World Wide Web.

Thesis (Ph. D.)--Institute of Paper Chemistry, 1959. / Includes bibliographical references (p. 88-119).

Over the last decade, millions of acres of western North American forest have been reduced to areas of standing dead trees following eruptions in bark beetle populations. This thesis provides up-to-date information on streamflow response to the recent bark beetle outbreak in subalpine forests of the Colorado Rockies. Streamflow and climate measures are evaluated in eight central Colorado catchments with long-term data records and varying levels of beetle outbreak. No detectable streamflow change is observed in 7 of 8 highly impacted catchments. A significant reduction in streamflow is observed in 1 highly impacted catchment and is likely driven by tree mortality and record warm temperatures. These findings deviate from expected results and have important implications for vegetation and streamflow change under a warmer climate.

Mercury (Hg) research in Ontario has centered on aquatic environments since fish can be a significant source of methylmercury for humans. Interactions between terrestrial and aquatic environments in regards to the Hg cycle have received less attention. This study focuses on the role of trees as a source and/or sink of Hg to the environment and the ap- plicability of deciduous trees as biomonitors for Hg. Leaf, bark and wood core samples were taken from a variety of deciduous species including Acer spp. (sugar, silver and red maple), Quercus spp. (red oak), Populus spp. (eastern cottonwood), and Salix spp. (willow) mainly from the Dorset and Kingston, ON regions. Examination of seasonal variation of Hg concentrations ([Hg]) in leaves indicated that average autumn leaf Hg ranged from 17– 45 ng/g and differed significantly among species. Leaf [Hg] increased with the growing season and roughly corresponded to regional atmospheric [Hg]. Microspatial trends in leaf [Hg], such as position on a branch, may increase variability. Tree cores from contami- nated and reference sites in the Kingston area showed that although dendrochronological techniques are not appropriate for estimating historical atmospheric Hg, they are useful for estimating the total Hg burden of a single tree. Populus and Salix, had the greatest bark and wood [Hg] with maximums reaching 18 ng/g. This was significantly higher than more inland trees such as Quercus and Acer, with maximum values of 7 and 1.2 ng/g for bark and wood [Hg]. Individual, average sized trees in this study contained a total of 0.4–2.6 mg of Hg. This study will provide forest and watershed managers with a better understanding of the importance of trees in the Hg cycle and the contribution of leaf and wood Hg to litterfall and waterbodies in southern Ontario. / Thesis (Master, Biology) -- Queen's University, 2007-12-18 23:48:21.97

The increasing frequency and severity of windthrow events affecting the forestry industry in New Zealand have raised important management issues surrounding the rate of colonisation of fallen trees by sapstain fungi and the time available for salvage harvesting before sapstain degradation limits potential economic returns. These fungi are known to be spread by a multitude of factors including wind, rain splash, harvesting processes and insect vectoring. Apart from the ecological interest in these interactions between fungi, plants and insects, sapstain fungi are also economically important because their hyphae discolour the sapwood and reduce the overall quality of the timber. The amount of time available to salvage harvest damaged trees is unknown, especially on seasonal and regional scales. Manipulative experiments were established in Pinus radiata forests to examine this seasonal and regional variation in sapstain attack following windthrow, and to investigate the importance of bark beetles as vectors of sapstain fungi. A range of methods were implemented to assess the role of bark beetles as vectors and to ascertain which sapstain fungi are associated with them. Experimental billet logs were caged to exclude beetles and subsequently analyse fungal attack in comparison with identical logs left exposed to beetles. In addition, individual beetles were sampled directly to determine whether they carried spores of particular fungal species and to assess the degree of association in vector-fungal dynamics. Finally, a novel application of DNA melt peak analysis was developed to investigate variation among the fungal communities associated with beetles potentially involved in vectoring sapstain spores. The moisture content of fallen trees was found to be the main factor regulating sapstain development, and when moisture content drops below 100% (on dry weight basis) sapstain fungi grew rapidly. The speed at which this level drops depends on the season, with much faster drying occurring in the warmer months of spring and summer. As a result, trees that fell in the previous winter or autumn did not develop significant sapstain levels until temperatures rose in the following summer, suggesting that storm-damaged trees that fall in winter can be left safely until just before the next summer before they are no longer suitable for salvage harvest. In New Zealand, the bark beetle species acting as vectors of sapstain fungi are not behaviourally adapted to colonisation of logs that are not in contact with the ground. Following windthrow events in pine forests, trees generally lie with their stems suspended above the level of the ground by their branches. As a result, under these circumstances, beetle colonisation of windthrown timber was low, and bark beetles were not a significant vector of stain. The caged and un-caged experimental log billets, however, were in contact with the ground, resulting in colonisation of the un-caged logs. In this case, bark beetles did play an important role in contributing to sapstain intensity, and the stain distribution within the logs mirrored that of the stain distribution. However, this effect may be due to the provision of access points for wind- or water-borne spores of the non-insect vectored stain fungus Diplodia pinea, or to the spread of hyphae through the tunnelling and feeding activities of beetles within the tree, rather than by bark beetles acting as vectors of spores. Bark beetles were confirmed as sapstain vectors with the isolation of seven different ophiostomatoid stain fungi from them, five of which were also found in wood. Finally, the development of a laboratory based, rapid species identification method was developed to identify fungal DNA. Melt peak analysis allowed the species-specific DNA melt temperatures to be compared with the melt temperatures of known species to be able to rapidly, and cheaply, identify an unknown species. Bark beetles are vectors of sapstain fungi in P. radiata forests, however the bark beetle species naturalised in New Zealand prefer to colonise wood when it is in contact with the ground. Following windthrow, trees are generally not attacked by beetles as they are held from the ground by their branches, leaving them to be stained predominantly be wind and rain dispersed stain fungus D. pinea. Stain did not occur until the moisture content of fallen trees dropped below 100%, which only happens in the warm months of summer and spring. In New Zealand, there are interactions between trees and bark beetles, and bark beetles with fungal pathogens from all around the globe resulting in a unique novel assemblage of species together for the first time. Understanding the dynamics of these species in their novel environment is crucial to effectively responding to potential pest threats.

The kiln drying of wood produces huge amounts of vapour. The vapour is released to the environment when the process purges some of the saturated hot air. The main environmental issue regarding the use of kiln drying process are the release of the water vapour which contains organic contaminants. Some of them are hazardous to human health. In addition, there are some wood particles which may released with the water vapour purging process. In this research, the vapour is condensed and analysed for its organic contaminants and their biodegradability. The result showed that the dominant contaminants present in the condensate were ethanol and methanol with the concentration of approximately 65 mg/L and 25 mg/L respectively. The average COD concentration of the condensate was 159 ± 40 mg/L. The analysis also showed that the contaminants were biodegradable. In order to treat the wastewater, a trickling filter process using bark chips as a support medium was used to treat an artificial wastewater. The artificial wastewater contained the dominant contaminant present in the wood drying condensate. In the experiment, different sizes of bark chips were used. In addition, the loading rate of the treatment system was varied by changing the flow rate and contaminant concentration. The 30 cm long trickling filter using bark chips varying between of 2.8 – 4 mm diameter as the support medium gave a maximum removal of 36.4 % with removal capacity of 8.34 kg COD/m³bed•day at a flow rate of 2.8 cm/min and average inlet COD load of 20.4 kg COD/m3bed•day. The trickling filter with bark chips varying between 5.6 – 8 mm diameter as the support medium was operated using variations in contaminant concentration and flow rate. The operation using different inlet concentration gave the highest removal rate of 13.5 kg COD/m3bed•day at average initial load of 84.9 kg COD/m³bed•day, flow rate of 2.8 cm/min and theoretical initial concentration of 680 mg/L. The trickling filter operated with flow rate variation showed the highest removal rate of 10 kg COD/m³bed•day at an average inlet load of 53.3 kg COD/m³bed•day and flow rate of 7.1 cm/min. The removal rate of the contaminants in treatment was limited. There is a number of possible explanations. First is the active surface area, which indicating the area where the contact between the biofilm surface and feed happened. The active surface area increased as the flow rate increased. Second is the residence time of the feed in the bed. The residence time of the feed varied with the flow rate. It decreased as the flow rate increased. Third is the influence of the contaminants in the feed. The presence of methanol and methanol in the feed inhibited each other’s degradation. The dimention of a full-scale biotrickling filter to be used in actual kiln was also estimated. The estimation was made based on the maximum removal rate and optimum flow rate obtained in the experiments. The result of the estimation showed to obtain significant removal, the required bed would have to be 2.35 m in diameter and 160 in height.