In urban areas, discharging wastewater into rivers is a common way to dispose of contaminants, and it is usually the most economical. Accurate information about how effluents are distributed in the receiving water body is desirable when designing industrial plants. Flow structures will be influenced by an effluent’s dilution processes during the mixing. Meanwhile, the cross-stream motions resulting from the streamline curvature can redistribute both the velocity and the shear stress, which favors the mixing behavior compared to a straight channel. However, the interactions between jet mixing behavior and the bend flow requires further investigation. In the present study, jets with different densities were discharged horizontally into a laboratory flume with a 135-degree open channel bend, and both the main and secondary flow behaviors in the bend were observed after the introduction of effluents. The acquired three- dimensional velocity data were used to validate numerical models of the effluent-bend flow. Numerical turbulence models such as the standard k-ε eddy viscosity model, non-linear k-ε model (Shih quadratic k-ε), and the k-ω SST (shear stress transport) model were employed to evaluate their accuracy. OpenFOAM was selected in the analysis for proposing better numerical models since it gives high-quality results to individualized complex fluid flows, and as an open source CFD software it can be beneficial to further develop and maintain. The first part of this study presents the implementation of the physical modelling of the proposed problems. Detailed descriptions of the experimental process were elaborated. Specifically, the three velocity components at four cross-sectional planes in the bend section were measured with and without saltwater jets by using the stereo Particle Image Velocimetry (PIV) ii technique in the laboratory flume. The experimental results show that the more pronounced effects with the jets were found at the beginning and exit of the bend. Although the jets had little effect on the maximum streamwise velocity, it was found that the occurrence of the negatively buoyant jets would affect the patterns and properties of the secondary flow in the bend. The second part of this study investigated the mechanisms underlying the two cells system, particularly when interacting with a discharged effluent jet. Detailed experimental data were used in interpreting the large center-region cell as well as small structures in the 135-degree open channel bend. A term-by-term analysis of the downstream vorticity equation was executed to investigate the various mechanisms underlying these cross-stream flow motions considering the influence of the negatively buoyant jets. The results indicated the generation and the dissipation of the streamwise vorticity with the effective terms of the vorticity equation. The third part of this study evaluated the performance of three different turbulence models with the experimental measurements. It can be concluded that fully 3D numerical models are capable of simulating the primary flow pattern in a strongly curved channel with the presence of a negatively buoyant jet. The comparison also shows that, although the outer bank cell was not predicted, the k-omega SST model can satisfactorily predict some of the smaller flow features in bend flow, such as the inner bank circulation cell and the overall form of the vorticity distributions. The results enable more reliable predictions for the characteristics and development of jets in a bend.