Degraded rivers and streams caused by disturbances have created a need for reliable tools to assess the ecological status of such ecosystems. Numerous methods and programs have been developed to assess ecological status using biological indicators, benthic macroinvertebrates are the most commonly used biotic indicator. The Hester-Dendy multi-plate sampler is a commonly used tool for sampling benthic macroinvertebrates, but its effectiveness under different environmental conditions has not been adequately tested. The aim of this study was to investigate if HD samplers assess the benthic macroinvertebrate community equally under different flow conditions. I investigated if the colonization of BMI in a constant flow differed from that of a variable flow (simulating a hydropeaking flow regime). This was studied using six aquariums, three as control with constant flows and three with variable flow conditions. One Hester-Dendy sampler and 50 benthic macro invertebrates from five different taxonomic orders were place in each aquarium. After five days of colonization the Hester-Dendy samplers were retrieved and benthic macroinvertebrates colonizing the Hester-Dendy samplers and still remaining in the aquariums were collected, preserved and analysed. Results showed that the mean sampling efficiency did not differ between the two treatments. On the other hand species diversity calculated from Shannon-Wiener index was significantly higher in the control treatment than in the variable flow treatment. The lower species diversity in the variable flow treatment is consistent with previous research on benthic macroinvertebrates affected by hydropeaking powerplants. These results can be seen as an indication of how a variable flow regime might affect the samples collected by HD samplers in a natural environment. As the artificial environments created are greatly different from a natural environment, this result might not therefore be representative in a natural environment. / Vattendrag kraftigt påverkade av antropogena störningarna har skapat ett behov av tillförlitliga verktyg för att kunna bedöma dessa vattendrags ekologiska status. Många olika metoder och program har utvecklats genom att använda olika biologiska indikatorer. Bentiska makroevertebrater är en av de vanligaste biotiska indikatorerna. En vanlig metod för att prov ta bentiska makroevertebrater är Hester-Dendy provtagaren. Syftet med denna studie var således att testa om provtagningseffektiviteten för Hester-Dendy provtagare påverkas olika av ett variabelt vattenflöde jämfört med ett jämnt vattenflöde. Detta studerades i sex akvarium, där tre akvarium agerade kontroll och utsattes för ett jämt flöde samt tre akvarium med variabelt flöde. En Hester-Dendy provtagare och 50 bentiska makroevertebrater från fem olika taxonomiska ordningar placerades i varje akvarium. Efter fem dagars koloniserings tid hämtades Hester-Dendy provtagarna och de bentiska makroevertebrater som koloniserade provtagaren såväl som akvariet bevarades i etanol och analyserades i labbet. Resultaten visade att den genomsnittliga provtagningseffektiviteten inte skilde sig åt mellan de två behandlingarna. Däremot var artdiversiteten beräknad med Shannon-Wiener-index signifikant högre i kontrollbehandlingen. Den lägre artdiversiteten i behandlingen med variabelt flöde stämmer överens med tidigare forskning om bentiska makroevertebrater påverkade av korttidsreglerande vattenkraftverk. Resultaten i denna studie bör ses som en indikation av hur ett varierande flöde kan påverka provtagnings effektiviteten hos en Hester-Dendy-provtagare. Detta med anledning av att den konstgjorda miljön som försöket utfördes i är vitt skilt från organismernas naturliga miljö vilket kan ha påverkat resultatet.

Invasions from coho salmon were first reported in the Cape Horn Biosphere Reserve (CHBR) in 2019 which is the most southern distribution registered to date. The CHBR is known for its high number of endemic species and unique biodiversity, such as the native fishes Galaxias maculatus and Aplochiton taeniatus. There are now three invasive salmonid species in the rivers of CHBR and are a potential threat to the native fish taxa. Stable isotope and gut content analysis were used to understand resource utilization by both native galaxiid and invasive salmonid taxa, as well as aquatic macroinvertebrates and riparian spiders. The natural laboratory study approach applied to this research, allowed for comparisons of differences within streams that contain conditions in which fish do not occur naturally, to sites in which high densities of invasive salmonid exist. Analysis of the trophic niche and diet in this study showed the importance of marine resource use by the native galaxiid and coho salmon juveniles supported with elevated δ15N and δ34S ratios. Diet analysis also confirmed there was the highest similarity between the coho salmon juveniles and the native fish. Altered behavior and habitat use was shown through the isotope and diet analysis for the galaxiid in snow melt streams which could be due to the high density of invasive salmonids in these streams. The invasive salmonids were found to impact aquatic macroinvertebrate populations, specifically larger bodied Trichoptera abundance. Aquatic insect emergence was negatively correlated to salmonid densities. The aquatic insect emergence revealed alterations with significantly higher biomass of aquatic insect emergence in upstream sites without invasive fish. Lastly, aquatic insect predator (Hydrobiosidae: Rheochorema sp.) exhibited a suppressed trophic position in rivers with invasive salmonids. An unexpected finding from the study was the refugium stream habitat conditions that has been shown to be important for conserving the native fish in the CHBR. We conclude that the impacts from invasive species are significant enough to disrupt lower trophic levels especially aquatic insects. The altered aquatic emergence and insect behavior have the potential to disrupt food webs and ecosystem functioning in the southernmost protected ecoregion in the world.

The Dallas-Fort Worth International (DFW) Airport is located in a densely urbanized area with one of the fastest-growing populations in the U.S.A. The airport property includes a large tract of "protected" riparian forest that is unique to the urban surroundings. This dissertation explores variables that influence the benthic macroinvertebrate community structure found in urbanized prairie streams that were initially assessed by the University of North Texas (UNT) Benthic Ecology Lab during four, non-consecutive biomonitoring studies (2004, 2005, 2008, and 2014) funded by the DFW Airport. Additionally, land use analysis was performed using 5-meter resolution satellite imagery and eCognition to characterize the imperviousness of the study area watersheds at multiple scales. Overall, flow conditions and imperviousness at the watershed scale explained the most variability in the benthic stream community. Chironomidae taxa made up 20-50% of stream communities and outperformed all other taxa groups in discriminating between sites of similar flows and urban impairments. This finding highlights the need for genus level identifications of the chironomid family, especially as the dominant taxa in urban prairie streams. Over the course of these biomonitoring survey events, normal flow conditions and flows associated with supra-seasonal drought were experienced. Prevailing drought conditions of 2014 did not negatively influence stream communities, allowing this study to capture the long-term natural (temporal) variability of urban prairie stream communities. Such long-term studies are imperative for discerning between stream impairment versus natural variation, especially as droughts become more frequent and severe.

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Measurements in paired stream reaches with and without livestock access in southwestern Virginia suggest that livestock exclusion practices installed on short, isolated stream reaches result in improved geomorphic and riparian vegetation condition, but do not significantly improve the benthic macroinvertebrate assemblage. Detailed longitudinal and cross-sectional surveys, pebble counts, and rapid geomorphic assessments were conducted on contiguous, paired stream reaches (5 pairs) with and without active livestock access across a range of time since livestock exclusion was implemented. In addition, bank characteristics were quantified by measuring groundcover biomass, shrub crown volume, tree density and diameter, soil bulk density, and particle-size analysis. Benthic macroinvertebrates were collected with a D-frame dip net and quantified using the Virginia Stream Condition Index (SCI), and other benthic macroinvertebrate metrics. We determined that: 1) small lengths of livestock exclusion can significantly increase channel depth and decrease the width to depth ratio, and increase groundcover vegetation growth, but do not significantly alter benthic macroinvertebrate assemblages; and, 2) qualitative geomorphic assessment results showed trends over time since exclusion (0 to greater than 50 years), but not in any of the other parameters evaluated. These observations suggest that a more targeted and holistic approach that addresses watershed-wide impacts must be implemented to restore aquatic habitat. (Key Words: CREP, stream channel morphology, livestock exclusion, agriculture, benthic macroinvertebrates, riparian buffers.) / Master of Science

Increased loading of dissolved ions (salinization) and trace elements from surface coal mining is a common alteration to headwater streams in the central Appalachian region. However, temporal and spatial trends of water quality and associated influences on biota in these stream systems have not been well-studied. To address this research need, I analyzed temporal trends in specific conductance, ion matrix, and benthic macroinvertebrate communities in 24 headwater streams, including 19 influenced by surface mining, from 2011-2019. There was limited evidence of recovery of water chemistry or macroinvertebrate communities in these streams, indicating lasting impacts from surface coal mining. Among benthic macroinvertebrates, Ephemeroptera and the scraper functional feeding group were most-impacted by chronic salinization in study streams. In addition, I analyzed spatial patterns of water chemistry in a subset of these streams using synoptic sampling of multiple constituents under baseflow and highflow conditions. Study results indicate that water chemistry is spatially dynamic and can be influenced by both groundwater dilution and inputs from tributaries. Lastly, I investigated patterns in selenium bioaccumulation across and within streams, from particulate matter to top trophic levels (i.e. fish and salamanders). I found that benthic macroinvertebrates had the highest concentrations of selenium in these ecosystems, with lower concentrations in salamander and fish species. However, there was limited evidence of longitudinal trends in bioaccumulation dynamics downstream of mining impacts. Collectively, this work indicates long-term (ca. decades) coal-mining influences but also highlights future research needs to better understand downstream impacts to water quality and biotic communities. / Master of Science / Surface coal mining affects water quality in central Appalachian headwater streams. However, long-term and downstream patterns of impacted water quality and potential effects on aquatic life have not been well-studied. To address this research need, I analyzed trends in water quality parameters and aquatic insect communities in 24 headwater streams from 2011-2019. There was limited evidence of recovery of water chemistry or aquatic life in these streams, indicating lasting impacts from surface coal mining. Certain aquatic insects including Ephemeroptera (mayflies) appear to be more impacted than others by long-term altered water quality. In addition to trends over time, I also analyzed downstream variation in water chemistry in a subset of these streams under baseflow conditions and after a rain event. Results indicate that water chemistry can vary greatly within a stream network and is influenced by tributary inputs and dilution from groundwater. Concentrations of the trace element selenium can also be elevated as a result of surface mining. This is of environmental concern because selenium can biomagnify, where concentrations increase in organisms higher in the food chain and can cause toxic effects. Here, I investigated selenium bioaccumulation patterns across organisms in the food chain and with distance downstream across six headwater streams. I found that aquatic insects had the highest concentrations of selenium, with lower concentrations in salamanders and fish. This work indicates that surface coal mining has longterm (ca. decades) effects on headwater streams, but also points to future research to better understand downstream impacts to water quality and aquatic life.

The goal of the Aquatic Indicators of Land Condition (AILC) project is to develop analytical tools that integrate land condition information with stream condition for improved watershed management within the United States Bureau of Land Management (BLM). Based on the goal of the AILC, two objectives for this study were: to determine the effect of four GIS-derived distance measurements on potential relationships between common BLM landscape stressors (mining and grazing) and changes in benthic macroinvertebrate community structure; and to assess the effectiveness of individual questions on a commonly-used Bureau-wide qualitative stream assessment protocol, the proper functioning condition (PFC) assessment. The four GIS distance measurements assessed for biotic relevance included: straight-line distance, slope distance, flow length, and travel time. No significant relationships were found between the measured distance to stressor and macroinvertebrate community structure. However, the hydrological relevance of flow length and travel time are logically superior to straight-line and slope distance and should be researched further. Several individual questions in the PFC assessment had statistically significant relationships with the final reach ratings and with field-measured characteristics. Two of the checklist questions were significantly related to the number of cow droppings. This may indicate a useful and efficient measure of stream degradation due to grazing. The handling and use of the PFC assessment within the BLM needs further documentation and examination for scientific viability, and the addition of quantitative measurements to the PFC in determining restoration potential would be desirable. / Master of Science

The Central Appalachian region is home to several resource-extraction industries, a host of globally unique aquatic wildlife species, and region-wide poverty. These overlapping circumstances make solving environmental issues in the region challenging. Of particular focus is the coal mining industry, both because of its prevalence and because of controversial methods of practice such as mountain top mining. One of the primary concerns in the region is the extirpation of sensitive aquatic macroinvertebrate species. Several studies have suggested the primary driver of this loss of biodiversity is due to increased conductivity in streams impacted by these mining practices. The reality is that several pollutant sources coexist in these Central Appalachian watersheds. Because of geographic isolation, many headwater communities lack proper sewerage and discharge directly into nearby waterways, compounding potential effects of upstream mining activities. Additionally, several legacy sites exist throughout the Appalachian region, both underground and surface mining in nature. To best mitigate ecological impacts of all of these pollutant types, relative contributions of each must be understood, as well as the nature of the pollution contributed by each. As a contribution towards this region-wide need for better information on pollution, the studies comprising this dissertation seek to better understand the composition of these different pollutant sources and their in-stream contributions to conductivity. The first paper found that these sources are indeed distinct in their inorganic ion make-up: surface coal mining was found to contribute primarily Ca, K, Ni, Se, and SO₄⁻², while untreated household waste (UHW) was primarily associated with P. HCO₃⁻ Mn, and Si were found to be associated with a legacy underground discharge. The second and third scientific studies included here analyzed conductivity's effect on Virginia Stream Condition Index (VSCI) versus other water quality and habitat parameters as well as the effect of specific ion suites on VSCI score. Findings indicated that excellent habitat extends species resilience against elevated conductivity, with passing VSCI scores found at conductivity in the 600-800 µs/cm range in cases of excellent habitat metric scores. Meanwhile, VSCI score suppression was highly related to surface mining-related inorganic ions (Ca, K, Mg, Ni, and SO₄⁻²), but also negatively correlated with ions related to UHW (P and Na). These results indicate the need for quantification of biological responses to specific ions in order to initiate targeted mitigate of pollutants in Central Appalachian watersheds. / Ph. D.

Prior studies have demonstrated Appalachian coal mining often causes elevated specific conductance (SC) in streams, and others have examined SC effects on benthic macroinvertebrate communities using point-in-time SC measurements. However, both SC and benthic macroinvertebrate communities exhibit temporal variation. Twelve Appalachian headwater streams with minimally impacted physical habitat and reference-quality physicochemical conditions (except elevated SC) were sampled ten to fourteen times each for benthic macroinvertebrates between June 2011 and November 2012. In situ loggers recorded SC at 15-minute intervals. Streams were classified by mean SC Level (Reference 17-142 S/cm, Medium 262-648 S/cm, and High 756-1,535 S/cm). Benthic macroinvertebrate community structure was quantified by the Virginia Stream Condition Index and other metrics. Structural metric differences among SC Levels and month of sampling were explored. Reference-SC streams exhibited significantly higher scores on most metrics, supporting previous findings that SC may act as a biotic stressor, even in streams lacking limitations from degradation of physical habitat or other physicochemical conditions. Temporal variation was greatest in Medium-SC streams, which had the most metrics exhibiting significant differences among months and the greatest range of monthly means for six metrics. Metrics involving % Plecoptera and/or % Trichoptera were not sensitive to elevated SC, as Leuctridae and Hydropsychidae exhibited increased abundance in streams with elevated SC. Best scores for benthic macroinvertebrate community metrics differed based on selected metric, SC Level, and month. Consequently, timing of sampling is important, particularly in streams with elevated SC because community metric scores may be impacted by dominant taxa life history patterns. / Master of Science