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Orientador: Abraham Hirsz Zimerman / Mestre

The saskatoon (Amelanchier Alnifolia Nutt.) is a woody shrub from the rose family. One of the main challenges to its culture is Entomosporium leaf and berry spot (ELBS) disease, which is caused by a hemibiothrophic fungus Entomosporium mespili (Sacc.). Due to the limited growth of the pathogen in vitro, we tested several culture media, and potato dextrose agar remained the best tested medium to grow the pathogen in vitro. E. mespili showed high variations in the morphology and sporulation of its cultures on PDA, which were not related to the geographic origin of the tested isolates. The amount of spores produced by the fungus in vitro was significantly high compared to its sporulation in nature. The use of liquid media such as potato broth was essential to produce enough mycelium for other experiments. The pathogen produced big spherical balls of mycelium where the nutrient and the aeration were sufficient. In absence of previous protocols to extract DNA from this pathogen, we tested several methods and determined a mixture of CTAB and SDS to be best for extraction of good quality DNA from E. mespili grown in vitro. Lyophilisation of E. mespili’s tissue and the addition of proteinase K to the extraction buffer reduced DNA degradation. To avoid the co-precipitation of polysaccharides with DNA, we used a high concentration of salt to precipitate polysaccharides, followed by a separation by phenol:chloroform:isoamyl (25:24:1). Molecular markers including Random Amplified Polymorphic DNA (RAPD) and Amplified Fragments Length Polymorphism (AFLP) revealed a high level of polymorphism in E. mespili. The artificial inoculation gave different results from natural infection, with a high level of diversity both on detached leaves and seedlings. According to our tests, growing E. mespili in vitro reduced its pathogenicity.

The saskatoon (Amelanchier Alnifolia Nutt.) is a woody shrub from the rose family. One of the main challenges to its culture is Entomosporium leaf and berry spot (ELBS) disease, which is caused by a hemibiothrophic fungus Entomosporium mespili (Sacc.). Due to the limited growth of the pathogen in vitro, we tested several culture media, and potato dextrose agar remained the best tested medium to grow the pathogen in vitro. E. mespili showed high variations in the morphology and sporulation of its cultures on PDA, which were not related to the geographic origin of the tested isolates. The amount of spores produced by the fungus in vitro was significantly high compared to its sporulation in nature. The use of liquid media such as potato broth was essential to produce enough mycelium for other experiments. The pathogen produced big spherical balls of mycelium where the nutrient and the aeration were sufficient. In absence of previous protocols to extract DNA from this pathogen, we tested several methods and determined a mixture of CTAB and SDS to be best for extraction of good quality DNA from E. mespili grown in vitro. Lyophilisation of E. mespili’s tissue and the addition of proteinase K to the extraction buffer reduced DNA degradation. To avoid the co-precipitation of polysaccharides with DNA, we used a high concentration of salt to precipitate polysaccharides, followed by a separation by phenol:chloroform:isoamyl (25:24:1). Molecular markers including Random Amplified Polymorphic DNA (RAPD) and Amplified Fragments Length Polymorphism (AFLP) revealed a high level of polymorphism in E. mespili. The artificial inoculation gave different results from natural infection, with a high level of diversity both on detached leaves and seedlings. According to our tests, growing E. mespili in vitro reduced its pathogenicity.

Thèse--Lettres--Paris, 1919. / Bibliogr. p. 633-657. Bibliogr. p. 356-366. Index.

Texte remanié de: Th. doct.--Méd. vétérinaire--Toulouse, 1995. / Bibliogr. p. 161-167.

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"Berry Fleming is a 'middle of the road Southern novelist' who has shown literary skill and a very real talent in his writings. There is a wide diversity of themes in his nine novels. The steady, quiet quality of his work and a strong Southern flavor give his books a clean warmness that makes them distinctive. Because of a personal interest in his works and their contribution to a better understanding of the South, the writer has undertaken this study of Mr. Fleming's life and his works. It also seemed advantageous to bring together in one place the biographical and bibliographical material on Mr. Fleming"--Introduction. / Carbon copy of typescript. / "May, 1958." / "Submitted to the Graduate Council of Florida State University in partial fulfillment of the requirements for the degree of Master of Arts." / Includes bibliographical references.

Note that equations and expressions has been omitted here and is instead presented in the work.  This thesis focuses on the geometric phase in pure and mixed quantum states. For the case of a pure quantum state, Berry's adiabatic approach (4.1.10) and Aharonov & Anandan's non-adiabatic generalization of Berry's approach (4.2.8) are included in this work. Mixed quantum state involves Uhlmanns approach (5.1.42), which is used extensively in Section 7 and Sjöqvist's et al. approach (5.2.22), used extensively in Section 6. Sjöqvist's approach states that the Uhlmann phase is an observable and provides the experimental groundworkusing an interferometer.􀀀This was later proven, by Du et al.[45] to reproduce experimental data (Figure 19) on page 56. The Uhlmann phase can be used to observe the behaviour of topological kinks. This was tested on 3 models, the Creutz-ladder, the Majorana chain andthe SSU-model. It is found that the Uhlmann phase is split into two regimes with the dividing parameter being the temperature. This temperature is called the critical temperature, Tc. If the temperatureis is below the critical temperature, the Uhlmann phase yields Π and if thetemperature is above the critical temperature, the Uhlmann phase yields zero. / Observera att ekvationer och andra uttryck har exkluderats här och är presenterade i själva arbetet. Detta examensarbete behandlar geometriska faser i rena och blandade kvanttillsånd. I rena kvanttillstånd finner man Berrys adiabatiska behandling av den geometriska fasen (4.1.10) och Aharonov & Anandan icke-adiabatiska generaliseringav Berry fasen (4.2.8). I det blandade kvanttillstånden har Uhlmann introducerat en förlängning av den geometriska fasen som sträcker sig till det blandadekvanttillstånden (5.1.42), detta finner man i sektion 7. Senare har Sjöqvistet al. introducerat ett alternativ till att angripa geometriska faser (5.2.22) som beskrivs i sektion 6. Sjöqvist konstaterade att Uhlmannfasen är observerbar,i kvantmekanisk mening, och presenterade ett  experimentelt upplägg där han visade just detta med hjälp av en interferometer. vilket senare bevisades av Du et al.[45] där de experimentella mätvärdena stämde överens med dem teoertiska (se figur 19 på sidan 56). Uhlmannfasen kan även användas för att observera topologiska "kink"-lösningar. Detta testades för 3 olika modeller; Creutz stege formationen, Majorana kedjan och SSU modellen. Det visade sig att Uhlmannfasen delades up i två regioner och var starkt beroende av temperaturen. Denna temperaturen kallades för den kritiska temperaturen Tc. Om temperaturen liggerunder eller över den kritiska temperaturen får man att Uhlmannfasen ger Π eller 0 respective.