No description available.

No description available.

No description available.

No abstract available. / Thesis (Ph.D.)-University of Natal, Durban, 1980.

The following blood group phenotypes and antigens were studied: Abantu , Ax, Ay, Bm, Bm-like, B3-like, "Bombay" Oh Le(a+b-), "Bombay" Oh Le(a-b-), para-Bombay, Mi(a+), Vw+, S-s-U-, Dantu, Gerbich-, P1H, STEM+, Rh :-34, Rhnu11 , Le(a-b-c-d-), McC(e+) and Wd( a+) and a new form of polyagglutination associated with haemoglobin M - type Hyde Park. The effect of inheriting a y, D--, Dc- or R1Lisa haplotype was also investigated. The following blood group antibodies were studied: anti-N in a person with type MN red cells, anti-hrs, anti-Rh34, anti-Jsb and anti-T. Type M red cells were confirmed to absorb anti-N and type N red cells not to absorb anti-M. A new technique was described for separating the two red cell populations in twin chimeras. Three XX/XX female dispermic chimeras with blood of two genetic types, two with patchy skin pigmentation, were identified. Reduced I and enhanced i antigen expression helped confirm a case of congenital dyserythropoietic anaemia type II. Oval red cells accompanying an r (dce) haplotype were found, and anti-Tja-like haemolysins were not detected in women about to abort. Aspects of haemolytic disease of the newborn due to ABO and Rh antibodies were discussed. Two new tests in which 2-mercaptoethanol was used to distinguish between IgG (7S) and IgM (19S) immunoglobulins were described. Blood group phenotype and gene frequency studies were made in Black, White, Indian and Coloured blood donors and the results were presented in 32 tables. Thirty monoclonal anti-A and 96 monoclonal antibodies for antigens in the ABO, MNSs, Rh, Lutheran, Kell, Lewis and Kidd systems and for other antigens were investigated for their activity and specificity. / Thesis (Ph.D.)-University of Natal, Durban, 1991.

Transfusion medicine is an important part of modern health care and the provision of reliably phenotyped red blood cells (RBC) is essential for safe and effective blood transfusions. For identification of many RBC antigens, monoclonal antibodies of either murine or human origin are available for use in agglutination assays, in which they perform as well as or better than the human polyclonal antibody preparations which they have replaced. However, the detection of some blood groups is still reliant on the use of human polyclonal antisera, which is a less reliable reagent source with respect to availability, batch to batch variation and bio-safety. The use of recombinant antibody and phage display technology for the discovery of new monoclonal antibodies with specificity for some of these RBC antigens has the potential to deliver an economical, unlimited supply of specific antibody reagents suitable for use in RBC phenotyping. Samples of human B cells from donors producing useful phenotyping antibodies were identified and transformed using Epstein Barr virus into lymphocyte cell lines. Antibody genes were obtained from the cell lines in the form ofRNA which was reverse transcribed, amplified by PCR and cloned into a phagemid vector system to generate several combinatorial antibody libraries. These antibody libraries were displayed on the surface of phage particles and subjected to antigen-driven selection by several rounds of phage display biopanning using soluble and cell based RBC antigens. In addition a large naIve library was biopanned against the same antigens in an attempt to isolate a wide range of antibodies suitable for blood typing. Several high quality combinatorial antibody libraries with respect to size (> 107 clones) and diversity were generated. Biopanning of recombinant libraries resulted in enrichment of phage antibodies specific for RBC antigens, and several clones were isolated which were shown to be specific for Duffy a antigen. The isolated antibodies would be ideal candidates for re-engineering into multivalent antibody molecules capable of direct agglutination of RBC and as such, have the potential to replace human polyclonal sera in the identification of Duffy a RBC antigen phenotyping.

Thesis(M.S.)--Auburn University, 2005. / Abstract. Vita. Includes bibliographic references.

Proefschrift--Amsterdam. / Summary in English. "Stellingen" leaf inserted. Includes bibliographical references (p. 121-122).

Thesis (Doctoral)--Ludwig-Maximilians-Universität zu München, 1934.

Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.