Os corantes benzazólicos fluorescentes por ESIPT são conhecidos pela sua grande estabilidade e variabilidade no comprimento de onda da sua emissão de fluorescência. Novos derivados, nomeados HBs, foram estudados como corantes para sangue por possuírem grupos sulfônicos na sua estrutura molecular, o que é característico dos corantes proteicos utilizados para a análise de sangue. Estudos sobre essa aplicação foram desenvolvidos nesse trabalho, visando estabelecer vantagens e desvantagens em comparação aos reagentes mais utilizados. Os resultados foram satisfatórios para essa utilização, sendo tão sensível quanto o Amido Black e permitindo a combinação de técnicas com diferentes reveladores, sendo eles: Amido Black, Cianoacrilato e Luminol. Este trabalho também estudou a síntese do Luminol reproduzindo metodologias descritas na literatura, além da síntese do isômero Isoluminol. A obtenção de um produto com alto grau de pureza é difícil. Como são necessárias várias etapas, o rendimento do produto não é alto. / The benzazole fluorescent dyes by ESIPT are known for their high stability and fluorescence emission wavelength variability. New derivatives, appointed HBs, were studied as blood enhancement dyes once they have sulfonic groups in its molecular structure, which is characteristic of protein dyes used for blood analysis. Studies about this application were developed in this work, in order to establish its advantages and disadvantages compared to commonly used reagents. The results were satisfactory for the referred use, being as sensitive as Amido Black and allowing for techniques combination with different developers: Amido Black, Cyanoacrylate and Luminol. This work also studied the Luminol synthesis by reproducing methodologies described in the literature, as well as the isomer Isoluminol synthesis. The obtaining of a product with high purity is difficult. As several steps are required, the product yield is not high.

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The causes of errors in the angle of impact calculation were investigated including the surface type, falling velocity and the method used to fit an ellipse to a bloodstain. As had been cited previously the angle of impact was generally underestimated, especially at acute angles and the reason for this was determined to be due to an overestimation of the length of a bloodstain. The surface type was found to significantly affect the accuracy of an angle of impact calculation and as the falling velocity increased, the angle of impact calculation became more accurate. High-speed photography was used to further investigate the formation of bloodstains on surfaces. It was found that the formation of the bloodstain varied depending on the surface type and the angle of the surface. Bloodstain pattern analysis involves the application of scientific techniques to reconstruct events that resulted in a bloodstain pattern. The position of the blood source in three-dimensional space is a fundamental element of this application. Currently little is known about the methods used by bloodstain pattern analysts to select bloodstains when determining the region of origin. Fourteen analysts worldwide were surveyed in order to ascertain this information. It was found that the methods used were variable and were often not based on scientific research. Research was therefore undertaken into bloodstain selection and in particular, which bloodstains should be selected for a region of origin analysis. As a result of these experiments, two sets of selection criteria were established, one for use when the region of origin is being calculated manually and one for when directional analysis is being used.

The causes of errors in the angle of impact calculation were investigated including the surface type, falling velocity and the method used to fit an ellipse to a bloodstain. As had been cited previously the angle of impact was generally underestimated, especially at acute angles and the reason for this was determined to be due to an overestimation of the length of a bloodstain. The surface type was found to significantly affect the accuracy of an angle of impact calculation and as the falling velocity increased, the angle of impact calculation became more accurate. High-speed photography was used to further investigate the formation of bloodstains on surfaces. It was found that the formation of the bloodstain varied depending on the surface type and the angle of the surface. Bloodstain pattern analysis involves the application of scientific techniques to reconstruct events that resulted in a bloodstain pattern. The position of the blood source in three-dimensional space is a fundamental element of this application. Currently little is known about the methods used by bloodstain pattern analysts to select bloodstains when determining the region of origin. Fourteen analysts worldwide were surveyed in order to ascertain this information. It was found that the methods used were variable and were often not based on scientific research. Research was therefore undertaken into bloodstain selection and in particular, which bloodstains should be selected for a region of origin analysis. As a result of these experiments, two sets of selection criteria were established, one for use when the region of origin is being calculated manually and one for when directional analysis is being used.

The success or failure of any criminal investigation often depends on the recognition of physical evidence left at a crime scene and the proper analysis of that evidence. Crime scenes that involve bloodshed often contain a wealth of information in the form of blood patterns, the location, and its cause. Any criminal investigation has specific tasks, from the time when the crime is reported to the reconstruction of crime scenes. A lot of work needs to be done. Once the investigation starts at the crime scene, BPA needs to be done at the crime scene and the investigating officer must identify this evidential tool. The investigating officer should not necessarily have specialised training in blood pattern analysis, but rather know when to use these experts at their bloody crime scenes. With the interviews and docket analysis done, the researcher found that this was a problem as the investigating officers, either had no knowledge on the subject of BPA or very little knowledge on this research. The purpose of this study was to determine the use of BPA to CSR, and for the investigating officer to realise that it is not just a bloody crime scene, but also contains a wealth of evidence. The researcher had two research questions. Once the investigating officer follows the objectives of criminal investigation, they should be able to have a strong case against the perpetrators. How could BPA be used in the reconstructing of a crime scene? The researcher wanted to bring it to the investigating officers’ attention that it is not just a bloody crime scene, but rather that it contains a wealth of evidence, which can give them a perspective of the movement of both the victim and perpetrator during the commencement of the crime. Regardless of the lack of knowledge of BPA, it is proposed that investigating officers are to be informed, either through station lectures or by yearly refresher workshops and courses of the evidential tool of BPA. When the bloody crime scene is reconstructed with the use of BPA, an insight of what transpired at the crime scene will help them to finalise their cases. For recommendations, it is proposed that investigating officers are to be trained in more in depth courses in criminal investigation as well as crime scene reconstruction and evidence collection using FSL. / Criminology and Security Science / M.Tech. (Forensic Investigation)