Permanent grassland is a complex of legumes, herbs, and grasses. They are an important plant component of the biosphere. They provide wide range of productive and non-productive functions. Climbing legumes are valuable species in grasslands. They are involved in higher quality of fresh matter and hay. The aim of this diploma thesis is to assess the influence of climbing legumes on the presence and coverage of other higher plant species in grassland, the height and productivity of vegetation in selected localities in the Prachatice district in 2017. The occurrence of legumes (climbing and non-climbing) has a significant impact on other species in the grassland community. The reason is the ability to fix aerial nitrogen and support grass species that require a good nitrogen supply. The obtained data were statistically processed.