Global ETD Search

1	Mechanism of regulation of the RPL30 pre-mRNA splicing in yeast Macías Ribela, Sara 13 June 2008 (has links) The mechanisms of pre-mRNA splicing regulation are poorly understood. Here we dissect how the Saccharomyces cerevisiae ribosomal L30 protein blocks splicing of its pre-mRNA upon binding a kink-turn structure including the 5' splice site. We show that L30 binds the nascent RPL30 transcript without preventing recognition of the 5' splice site by U1 snRNP but blocking U2 snRNP association with the branch site. Interaction of the factors BBP and Mud2p with the intron, relevant for U2 snRNP recruitment, is not affected by L30. Furthermore, the functions of neither the DEAD-box protein Sub2p in the incipient spliceosome, nor of the U2 snRNP factor Cus2p on branch site recognition, are required for L30 inhibition. These findings contrast with the effects caused by binding a heterologous protein to the same region, completely blocking intron recognition. Collectively, our data suggest that L30 represses a spliceosomal rearrangement required for U2 snRNP association with the nascent RPL30 transcript. poylpyrimidine tract (Ppy tract) branch site (BS) ' splice site (3'ss) 5' splice site (5'ss) intron L30 RPL30 U2 snRNP U1 snRNP transcription regulation of splicing spliceosome assembly splicing gene expression 57