An investigation was conducted into gall induction in the leaves of Salix fragilis L. var. russelliana (Sm.) Koch by Pontania proxima (Lepeletier) (Hymenoptera: Tenthredinidae). The work was divided into four parts:- a) Reviews of the available literature were undertaken on: hypotheses concerning cecidogenesis of galls initiated by insects, the history of cecidology as it related to galls formed by insects, the ontogeny and morphology of galls produced by sawflies, ecological aspects of gall biology and the effects of galling on the host plant. b) Methods of rearing both insect and host were established; these resulted in an extension of the insect's flight period from five to eight months with three broods per year instead of the usual two. Further work established the topological specificity of oviposition. Preliminary studies were completed on the tissue culture of Salix leaf-disc explants and the fusion of protoplasts released from normal and galled tissue. c) Using light and electron microscopy, a study was made of the reproductive system and associated glands of Pontania proxima females, together with the first stages of the procecidium they initiated. Using these techniques, no microorganisms, viral or otherwise, were observed in the gall, in the lumina of the reproductive system and associated glands or in surrounding tissues of the insect. Evidence for a secretory role for the lateral oviducts was found. A structure termed the vaginal valve was described and it was hypothesized that this functioned to separate the fluids produced by the accessory glands from the contents of the oviduct, until oviposition. In the plant, it was observed that the gall effect was limited and that the presence of an egg or larva was not required for the formation of a procecidium. Gall growth was mainly due to periclinal divisions of the provascular tissues of the leaf. d) A bioassay, based on microinjection techniques, was developed .This demonstrated that the cecidogen was contained in the colleterial fluid produced by the accessory glands. Further analysis showed that the cecidogen had a molecular weight of less that 3 kDa.

Brittle cornea syndrome (BCS) is an autosomal recessive, multisystemic connective tissue disorder characterised by extreme corneal thinning and fragility. Mutations in transcription factors ZNF469 and PRDM5 cause BCS types 1 and 2, respectively. Both genes are believed to regulate the transcription of extracellular matrix (ECM) components, particularly fibrillar collagens, and are suggested to act on a common pathway. Molecular diagnosis is available for affected patients, and those at risk of being heterozygous carriers. Chapter 3 presents the identification of mutations in ZNF469 in 14 families with BCS, and evidence for the downregulation of ECM-associated transcripts in skin fibroblasts from patients with ZNF469-associated disease by Q-PCR.Chapters 4 and 5 focus on PRDM5-associated disease. Chapter 4 highlights previously undescribed and potentially phenotype-related aspects of PRDM5- associated BCS. In chapter 4, a potential role for PRDM5 in development of Bruch’s membrane is suggested, by the observation of significantly reduced expression of major components of Bruch’s membrane, including collagens types I, III, and IV in patients with PRDM5-associated disease using immunohistochemistry. A first description of PRDM5 expression in the human eye is also presented in chapter 4. In chapter 5, a potential role for PRDM5 in retinal vasculogenesis is suggested. PRDM5-related disease also offers an in vivo opportunity to observe a subset of epigenetic regulatory mechanisms in an inherited eye disease, providing mechanistic insights, presented in chapter 5. Examination of PRDM5 interaction partners by pull-down and mass spectrometry reveals the diminished interaction of a PRDM5 construct carrying a BCS-associated mutation with repressive complexes, and, through studies on fibroblasts and retinal tissue from patients, we suggest a role for dysregulation of the repressive histone mark H3K9 di- methylation in vivo. These findings suggest a role for a molecular network surrounding dysregulated H3K9 di-methylation in PRDM5-associated disease. Finally, chapter 6 expands the study of a rare disease into more common diseases investigating the role of genetic variations in ZNF469 and PRDM5 in keratoconus, an ocular disorder resulting in progressive corneal thinning. I identified enrichment of rare potentially pathogenic alleles in ZNF469 in 12.5% of keratoconus patients, highlighting ZNF469 as the most significant genetic factor responsible for keratoconus identified to date. In conclusion, this study of a rare disease, BCS, has provided translational research insights (chapter 3), functional insights (chapter 4) mechanistic insights (chapter 5) and has expanded into other, less rare, diseases (chapter 6).

When a brittle material is loaded to the limit of its strength, it fails by nucleation and propagation of a crack. The conditions for crack propagation are created by the concentration of a long-range stress field at an atomically sharp crack tip, creating a complex and strongly coupled multiscale system. This thesis reports the results of multiscale simulations of the brittle fracture of silicon on the (111) cleavage plane. The simulations are made possible by combining a quantum mechanical description of the processes taking place near the crack tip with a classical atomistic model that captures the long-range elastic relaxation. The 'Learn on The Fly' technique is used to couple the quantum and classical models, allowing accurate quantum forces to be combined with classical forces using a simple adjustable potential to give stable dynamics. The simulations predict that fracture is unstable on the (111) plane at low speeds; conventionally this has been thought of as the most stable crack plane. The instability is caused by a crack tip reconstruction which triggers a positive feedback 'sinking' mechanism leading to macroscopic, experimentally observable corrugations. Recent experiments have observed crack surface features consistent with these predictions. The instability is the first example in a crystalline material of a fracture instability which onsets below a critical velocity, and shows how subtle atomistic details at the crack tip can control the qualitative macroscopic fracture behaviour.

[pt] Este trabalho lida com uma teoria de dano contínuo, desenvolvida em um contexto termodinâmico, capaz de realizar uma descrição macroscópica da degradação de um material induzida pela deformação em estruturas elásticas frágeis (isto é, falha ocorre sem deformações permanentes). Na modelagem, supõe-se que a energia livre de Helmholtz não depende apenas da deformação e da temperatura absoluta, mas também da variável dano e de seu gradiente. Além disso, para levar em conta os efeitos microscópicos, a potência dos esforços internos não depende apenas da velocidade e de seu gradiente, mas também da taxa de evolução do dano e de seu gradiente. Apesar da sofisticação mecânica da teoria, uma técnica numérica simples, baseada no método dos elementos finitos, é proposta para aproximar a solução dos problemas matemáticos não lineares resultantes. Nestes problemas o acoplamento entre as variáveis dano e deformação é contornado por meio da técnica de partição dos operadores. Para validar o modelo e investigar as características principais do método numérico, diversos exemplos são apresentados para mostrar que os algoritmos utilizados não são sensíveis à malha (mesh dependent). / [en] The present work deals with a continuum damage theory, developed within a thennodynamical framework, able to perform a macroscopic description of material degradation induced by deformation in brittle elastic structures (i.e. failure occurs without permanent deformations). In the modeling, the Helmholtz free energy is supposed to depend not only on the strain and on the absolute temperature but on a damage variable and its gradient as well. Besides, to account for microscopic effects, the power of internal forces depends not only on the velocity and its gradient, but also on the damage velocity and its gradient. Despite the mechanical sophistication of the theory, a simple numerical technique, based on the únite element method, is proposed to approximate the solution of the resulting non linear mathematical problems. The coupling between damage and strain variables in these problems is circumvented by means of a splitting technique. In order to analyse the physical coherence of the model and to access the main features of the numerical method, a number of examples is presented showing that the numerical computations are not mesh dependent.

Research areas within the automotive industry are dedicated to reducing the weight and emissions of vehicles. Through the application of lightweight materials, such as polymers, fuel consumption and production costs can be decreased. Therefore, understanding the mechanical responses and failure mechanisms of these materials is significant to the development and design of vehicular structural components. Experimental tests were performed to capture the time, temperature, and stress state dependence, as well as failure mechanisms and large-strain mechanical responses of polypropylene (PP) and copolymer polypropylene (co-PP). Alongside studying the mechanical responses of PP and co-PP, the deformation mechanisms associated with the ductile and brittle failures were also examined. By applying an Internal State Variable (ISV) model, the mechanical behavior of PP and co-PP under various strain rates and temperatures was predicted. Phenomenological, mechanics based failure criteria were also applied to the model to predict the ductile or brittle failure of the materials.

<p> Small shear zones located northwest of the Grenville Front Mylonite Zone exhibit both a brittle and ductile deformation history. Textures reveal that an earlier mylonitic rock has been overprinted by one which demonstrates textures typical of brittle cataclasis.</p> <p> ductile deformation has occurred under greenschist facies conditions, while a later, brittle event has occurred below lower greenschist temperatures. These produce high shear strain values which lie between 14.44 and 10.79.</p> <p> Upper and lower age limits for the initiation of shear zones have been determined as being prior to the emplacement of pegmatite dykes, and up until the formation of brittle-ductile shear zones found locally. Thus, they have developed between 1400 ± 50 Ma and approximately 1100 + ? Ma.</p> / Thesis / Bachelor of Arts (BA)

Analyses of fluid inclusions and microstructures within the Papoose Flat pluton were used to investigate the chemistry and temperatures of fluids circulating with the pluton during cooling. Based on previous microstructural analyses, the interior of this late Cretaceous granitic to granodioritic pluton has been divided into three domains: i) a central core characterized by magmatic microstructures, ii) a middle domain of high temperature (>500°C) solid-state deformation, and iii) an outermost domain characterized by relatively low temperature (<5000°C) solid-state deformation. According to previously published anisotropy of magnetic susceptibility analyses and pluton cooling models, plastic flow occurred in both the outer part of the pluton and within its aureole rocks while the core of the pluton was still molten. Solid-state deformation is proposed to have stopped when the pluton interior cooled through its solidus less than 100,000 years after magma emplacement. Microstructural analysis of samples from all three domains confirmed the transition from magmatic flow in the core of the pluton to solid-state deformation at the pluton margin. However, weakly developed solid-state microstructures overprint the dominant magmatic microstructures in samples from the core domain. The existence of solid-state microstructures in all three domains indicates that deformation continued during and after crystallization of the interior of the pluton. Two phase, low salinity (< 26 wt% NaCl equivalent), liquid-rich aqueous fluid inclusions predominate within both quartz and feldspar grains in all samples. Throughout the pluton, the majority of fluid inclusions are hosted by deformed grains. Feldspar-hosted primary inclusions are associated with sericitic alteration. Inclusions were also observed in feldspar as secondary or pseudosecondary inclusions along fractures. Inclusions in quartz are frequently found near lobate grain boundaries or near triple junctions; linear pseudosecondary inclusion assemblages are commonly truncated against lobate boundaries between adjacent quartz grains, indicating that discrete microcracking events occurred during plastic deformation. Homogenization temperatures overlap for all three microstructural domains. Coexisting andalusite and cordierite in the contact aureole, and the intersection of the Mus + Qtz dehydration reaction with the granite solidus, indicate trapping pressures between 3.8 and 4.2 kb. Ninety-eight percent of the calculated fluid inclusion trapping temperatures at 3.8 - 4.2 kb are below the granite solidus of 650°C. Seventy-six percent of the trapping temperature data fall within the more restricted range of 350-500°C; i.e. at temperatures which are lower than the commonly cited brittle-ductile transition temperatures for feldspar at natural strain rates, but above those for quartz. No correlation could be established between trapping temperatures and either host mineral or microstructural domain within the pluton. The similar, relatively low trapping temperatures indicate that the majority of inclusions preserved in all three domains were trapped during the late low strain magnitude stages of solid-state deformation. The most common fluid inclusion trapping temperatures (400-500°C) in all three microstructural domains are similar to the deformation temperatures indicated by microstructures and crystal fabrics in the outer part of the pluton; these trapping temperatures are obviously lower than temperatures associated with contemporaneous solid state and magmatic flow in the pluton interior. The similar trapping temperatures within the pluton core and margin must indicate that the inclusion-trapping event migrated from the margin to the core of the pluton as it cooled, because fluid inclusions would rapidly equilibrate to a density appropriate for the PT conditions of their host minerals. / Master of Science

The work described herein examines the fracture behavior of steels used in bridge applications. As part of Transportation Pooled Fund (TPF) Project 5-238, Design and Fabrication Standards to Eliminate Fracture Critical Concerns in Steel Members Traditionally Classified as Fracture Critical, researchers aim to take advantage of advances made in both steel production technology and in the field of fracture mechanics. Testing and analysis of both conventional and High Performance Steel (HPS) grades of bridge steel was conducted as part of this study. This includes both Charpy V-Notch testing, as well as more rigorous elastic-plastic fracture toughness testing. Analysis includes the application of the master curve methodology to statistically characterize fracture behavior in the ductile to brittle transition region. In addition, a database of historic bridge fracture toughness data was compiled and re-analyzed using plasticity corrections to estimate elastic-plastic fracture toughness. Correlations between Charpy V-Notch impact energy and fracture toughness, which forms the basis for the current material specification, were also examined. Application of fracture toughness characterization of both new and historic data results in updated methodologies for addressing fracture in bridge design. / Ph. D.

The effects of lithology, fracturing, and gouge zone mineralization on the geophysical properties of fault zones are not very well understood. In situ seismic data collected over the exhumed San Gregorio Fault at Moss Beach, CA were used to relate in situ compressional wave velocity to internal fault zone properties. This active strike-slip fault is exposed in cross section on an uplifting and actively eroding wave-cut platform. It cuts shallow marine sediments that have been buried to depths of a few kilometers. The unweathered exposure containing seawater makes it a unique analog of subsurface faults. Previous structural analysis over this exposure observed damage caused by faulting over a ~100 m wide zone in cross-section. The fault zone is centered at a 10-17 m wide clay-rich fault core flanked by a ~30 m wide brecciated gouge zone. These gouge zones are bordered on either side by 30-40 m wide fractured zones. Resolving to a scale of a few meters, the seismic survey produced a continuous P-wave velocity profile analogous to a horizontal well log across the fault. Lateral variations in the velocity profile correlate exactly to previously mapped fault zone structure. The clay core and adjacent brecciated gouge create a ~50 m wide very low velocity zone, 25-50% slower than the surrounding host rock. Fractured bedrock on either side of the core causes a wider zone of 5-10% slow velocity, for a total fault signature ~100 m wide. Fault parallel fracture anisotropy was observed in the fractured zones, but surprizingly anisotropy was not observed in the strongly foliated gouge zones. The field measurements differ significantly from laboratory measurements at zero pressure and in some cases from expected values for saturated rock of this porosity, perhaps due to biased rock sampling, the long wavelength effects of macrofractures, frequency dispersion, and partial saturation. The velocity profile is similar in width and consistent in velocity contrast to low S-wave velocity zones derived from fault zone guided waves in other strike-slip faults. The traveltime delay across the fault zone is not large enough to cause the 2-3 km wide crustal low velocity zones modeled by refraction studies. Synthetic reflection seismograms in the typical frequency range show that the fault zone acts as a thick bed or as a constructively interfering thin bed. The models suggest that very large reflection coefficients observed across accretionary prism faults can be explained by fracturing, brecciation and clay content without elevated pore pressures. Comparison with a refraction study across the Punchbowl Fault shows a similar structural zonation of these two well-studied examples of brittle fault zones. This suggests that high-resolution seismic velocity models can be used to directly interpret internal deformation structure of brittle faults. / Master of Science

La bonne ténacité des aciers pour gazoducs aux basses températures est nécessaire pour éviter la propagation de fissures de manière catastrophique. Cette étude vise à améliorer la compréhension physique et l'évaluation quantitative du comportement à rupture des aciers pour gazoducs laminés à chaud, en nous intéressant plus particulièrement aux températures au pied de la transition ductile-fragile .La résilience de ces aciers est généralement validée à l'aide d'essais dits drop weight tear tests (DWTT), après lesquels le faciès de rupture doit contenir moins de 15% de zone fragile. Pour les aciers mis en forme par laminage thermomécanique (TMCP), des ruptures fragiles hors plan, comme le délaminage (qui se propage dans le plan de laminage de la tôle), et la rupture fragile en biseau le long des plans dits thêta (inclinés de 40° autour de la direction de laminage par rapport au plan de laminage) apparaissent dans la transition ductile-fragile. Ces modes de rupture, observés lors des essais de résilience (DWTT, Charpy) et de ténacité (CT), affectent la résistance à la rupture de ces aciers.L'anisotropie de l'écoulement plastique, puis celle de la sensibilité à la rupture par clivage ont été caractérisées en fonction de la température, à l'aide d'essais de traction sur des éprouvettes lisses et entaillées conçues pour cette étude. L'analyse mécanique de ces essais à l'aide de calculs par éléments finis a permis de déterminer des contraintes critiques de clivage dans les directions perpendiculaires au plan de laminage et aux plans thêta. Les valeurs obtenues dans ces directions sont de 25% inférieures à celles correspondant aux directions de laminage et travers long.L'anisotropie de la contrainte critique de clivage a été quantitativement corrélée à l'anisotropie de microtexture du matériau. Des entités appelées « facettes de clivage potentielles » ont été définies et mesurées dans cette étude, comme des régions contenant un plan {100} défavorablement orienté et dans lesquelles les fissures de clivage se propagent sans être arrêtées. Par exemple, un plan contenant 20% de facettes de clivage potentielles aurait une contrainte critique de clivage de 20% moins élevée qu'un plan présentant seulement 10% de facettes de clivage potentielles.La taille et la forme de ces facettes de clivage potentielles évoluent avec la déformation plastique. Par conséquent, la contrainte critique de clivage est affectée par l'historique de déformation. Dans le cas du délaminage, les facettes de clivage potentielles s'allongent au cours d'un chargement dans la direction travers long, conduisant à une augmentation de leur taille effective et par conséquent à une diminution (qui peut atteindre 30%) de la contrainte critique de clivage dans le plan de la tôle. Cette diminution facilite in fine l'apparition du délaminage. De plus, la présence de micro-fissures ductiles facilite la rupture par délaminage en modifiant l'état de contrainte local. Un critère a ainsi été proposé pour prédire numériquement l'amorçage du délaminage dans des éprouvettes de traction et/ou de résilience Charpy.L'application de cette approche à des échantillons traités thermiquement et à des échantillons pré-déformés a montré que la sensibilité au délaminage pouvait être contrôlée en modifiant la texture locale initiale du matériau. / High toughness of pipeline steels at low temperature is required to avoid catastrophic propagation of brittle crack. The aim of the study is to improve physical understanding and quantitative assessment of the toughness behavior of hot-rolled pipeline steels, focusing on the lower part of the ductile to brittle transition temperature range.The impact toughness of these steels is commonly validated using drop weight tear tests (DWTT), on the basis of fracture surfaces that must exhibit less than 15% of brittle fracture appearance. In thermomechanical control processed steels, brittle out-of-plane cracks such as delamination (which propagates along the rolling plane), and brittle tilted fracture (BTF) along theta-planes (tilted around RD by 40° with respect to rolling plane), have been characterized in the ductile to brittle transition temperature range, for both industrial (DWTT) and laboratory Charpy impact tests. In both cases, as well as in fracture toughness tests, such brittle out-of-plane cracking has been shown to impair the impact toughness.The anisotropy in plastic flow and sensitivity to cleavage fracture has been characterized as a function of temperature, by using tensile tests on specifically designed smooth and notched specimens. From finite element mechanical analysis of these tests, critical cleavage stresses normal to the rolling plane and the theta-plane are considerably lower (around 25%) than for planes normal to the rolling and transverse directions.The anisotropy in critical cleavage stress has been quantitatively correlated to microtexture anisotropy. So-called “potential cleavage facets” have been defined and measured in this study, as regions with unfavorably oriented {100} planes, which are taken as unit crack paths for cleavage propagation. A sample containing 20% of potential cleavage facets had a critical cleavage stress 20% lower than a sample with only 10% of potential cleavage facets.The size and shape of these potential cleavage facets evolve during plastic deformation. Therefore, the critical cleavage stress was found to be affected by plastic strain history. In the case of delamination, potential cleavage facets along the rolling plane were elongated during loading, their area was increased and the corresponding critical cleavage stress decreased by around 30% with respect to the undeformed case. This made delamination cracking easier. Moreover, the presence of a ductile crack at the initiation site of delamination locally modified the stress state and also facilitated delamination occurrence. A criterion has been developed to numerically predict the onset of delamination in tensile and Charpy specimens.Application of this approach to heat-treated and to prestrained specimens eventually showed that it was possible to modify the sensitivity to delamination by strongly modifying the initial microtexture anisotropy.