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1	Histone modifications after DNA damage affect survival in Schizosaccharomyces pombe / Rajput, Abdul Mateen January 2010 (has links) S. cerevisiae Ada2 and Bre1 has a role in histone post-translational modifications. Deletion of these genes causes deficiency in acetylation (Ada2) or ubiquitination (Bre1) of histones. Further, mutants lacking these genes or homologous genes showed different phenotypes in human and S. cerevisiae while treated with DNA damaging agents 4-NQO and MMS. Bre1 deficient cells showed 4-NQO sensitivity in S. cerevisiae and resistance in human cells. Since it has been shown that S. pombe is more close to mammals in chromatin regulation we wanted to examine S. pombe response against MMS and 4-NQO. By homologous recombination, genes were deleted and mutants were treated with different concentration of both the genotoxins. In accordance with a previous study, Ada2Δ showed sensitivity to MMS while Brl1Δ & Brl2Δ grew as wild type. Surprisingly, unlike S. cerevisiae, S. pombe showed resistance to 4-NQO and has a phenotype similar to the one found in human cells. Epigenetics DNA damage S. pombe yeast histone modification chromatin life sciences gene deletion gene tagging pcr mms 4-nqo brl1 brl2 Epigenetik DNA-skada S. pombe jäst histon modifiering kromatin biovetenskap gendeletion gen taggning PCR MMS 4-nqo brl1 brl2 Cell and Molecular Biology Cell- och molekylärbiologi