Thesis (D. Min.)--Midwestern Baptist Theological Seminary, 2002. / This is an electronic reproduction of TREN, #054-0180. Includes bibliographical references (leaves 122-124).

Thesis (M.A.)--State University of New York at Buffalo, 1986. / Includes bibliographical references (leaves 131-143). Also available in print.

Thesis (Ph. D.)--Columbia University, 1937. / Vita. Published also as Teachers College, Columbia university, Contributions to education, no. 715. Bibliography: p. [65]-74.

Made available in DSpace on 2014-06-11T19:31:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-02-27Bitstream added on 2014-06-13T19:41:03Z : No. of bitstreams: 1 santos_das_dr_jabo.pdf: 2572223 bytes, checksum: aea5701db88f8bf27923f6c9ed630f4e (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Este estudo utilizou 15 fêmeas bufalinas, provenientes de abatedouros frigoríficos dos estados do Maranhão, São Paulo e Paraná para estudo morfológico, macroscópico, microscópico e morfométrico das papilas mamárias. O material para estudo macroscópico foi pesado, medido e fotografado, e os resultados do peso papilar foram de: cranial direita (PCD) 20, 95g; caudal direita (PCaD) 21,49g; cranial esquerda (PCE) 23,52g e para caudal esquerda (PCaE) 21,35g. Os valores médios para comprimento do ducto papilar, número de pregas e comprimento da cisterna papilar foram de: 0,67cm; 5,5 e 2,17cm para PCD; 0,62cm; 6,0 e 2,26cm para PCaD; 0,71cm; 6,0 e 2,26cm para PCE e 0,74cm; 5,7 e 2,57cm para PCaE, respectivamente. A análise de correlação de Pearson indicou não existir relação entre o peso corpóreo e o peso glandular. Fragmentos das regiões proximal, média e distal foram colhidos e fixados em solução aquosa de formol 10%, cortados com 10, 20 e 40mm e corados em Azan, Picrocírus F3BA e Weigert-Van Gieson, para a análise mesoscópica. Cortes com 5mm corados em HE, tricrômico de Masson foram utilizados para a análise histológica e morfométrica. A estrutura histológica da papila mamária em búfalas mostrou-se similar aquela dos bovinos, a camada muscular é formada por fibras em duas disposições, a interna circular e a externa longitudinal, e com epitélio cúbico biestratificado em quase toda a sua extensão. Na porção média da papila a camada mais desenvolvida é a muscular com 22,90mm de espessura, a qual possui grande quantidade de tecido conjuntivo denso (7,10 mm). / Fifteen Murrah female buffaloes, proceeding from abattoirs of Maranhão, São Paulo and Paraná states, were used in this study and submitted for morphologic, macroscopic, microscopic and morphometric analysis of the mammary papilla. The material for macroscopic study was weighed, measured and photographed, the results for papillary weight had been: cranial right (PCD) 20.95g; caudal right (PCaD) 21.49g; left cranial (PCE) 23.52g and for left caudal (PCaE) 21.35g. The average values for the papillary duct length, number of folds and length of the papillary cistern had been respectively for PCD: 0.67cm, 5.5, 2.17cm; PCaD: 0.62cm, 6.0, 2.26cm; PCE: 0.71cm, 6.0, 2.26cm and PcaE: 0.74cm, 5.7, 2.57cm. The analysis of de Pearson correlation coefficient test between corporeal weight and the glandular weight indicated not relation existence. Proximal, meddle and distal tissue samples had been collected and fixed in 10% formol watery solution and cut with 10-20-40mm and stained in Azan, Picrosirus F3BA and Weigert-Van Gieson, for the mesoscopic analysis. Cuts of 5mm and had stained in HE and Masson's trichrome were submitted to the morphologic and morphometric analysis. Buffalo's' mammary papilla structure revealed to be similar to the described for bovines, the muscular layer was composed by two bundles of smooth muscle fibers, the external circular layer and the longitudinal external one. The mucosa was bi-stratified cuboidal ephitelia in almost all its extension. The papilla meddle portion muscular layer was 22.90mm of thickness which contained large amount of connective tissue (7.10mm).

Made available in DSpace on 2014-06-11T19:33:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-07-10Bitstream added on 2014-06-13T20:24:58Z : No. of bitstreams: 1 gomes_ra_dr_jabo.pdf: 423622 bytes, checksum: 4197737c8e3e3fefdbe077c8f459f240 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo do presente estudo foi analisar a resposta imune humoral, pelo monitoramento dos anticorpos anti-Babesia bovis, anti-Babesia bigemina e anti-Anaplasma marginale, em búfalos (Bubalus bubalis) naturalmente infectados. Para esta pesquisa, utilizaram-se amostras de soro e de colostro/leite de búfalas adultas do periparto aos 11 meses após, e de soros dos seus bezerros, durante o primeiro ano de vida nos anos de 1999/2000 e 2005. Para determinar o perfil da resposta imune humoral destes animais, utilizou-se o método ELISA indireto e os dados foram apresentados e analisados como a média de um grupo de animais, em diferentes faixas etárias e, individualmente. Após a leitura e interpretação dos dados, os resultados dos animais analisados em grupos apresentaram baixa concentração de anticorpos, ou seja, abaixo do ponto de corte (D.O. = 0,265 e NE=3) de anticorpos anti-A. marginale nos soros, durante os primeiros 90 e 105 dias após o parto e nascimento, respectivamente, para búfalas e seus bezerros. Em seguida, a concentração de anticorpos anti-A. marginale no soro dos bezerros búfalos aumentou ligeiramente acima do ponto de corte e manteve-se assim até atingirem aproximadamente um ano de idade, indicando uma imunidade adquirida, após o contato com a bactéria. Nas búfalas ocorreu soroconversão (NE acima de 3) para Babesia de ambas as espécies, por quase todo o período analisado, com uma elevação acentuada (NE=4 a NE=6) entre os dias 91 e 335 dias após o parto, fato não verificado para os bezerros, no mesmo período. No colostro/leite, os anticorpos anti-B. bovis e anti-B. bigemina foram detectados nos primeiros sete dias pós-parto, mas não foram observados no teste anti-A. marginale. Quando os animais foram analisados individualmente (duas búfalas e seus bezerros), observou-se em um dos bezerros, uma forte imunidade humoral... / The aim of the present study was to analyze the humoral-immune response of water buffaloes (Bubalus bubalis) naturally infected with Babesia bovis, B. bigemina and Anaplasma marginale. For this work, colostrums/milk and blood samples were weekly, fortnightly and monthly harvested prior and after partum (buffalo cows) and from birth to 365 days after birth (buffalo calves). The antibodies in the colostrums/milk and serum samples from these animals were determined using an ELISA indirect method and the data were analyzed as a mean of a group of animals with matched ages during the period of 1999/2000 or individually during the year of 2005. The data from animals analyzed in group showed that the antibodies against A. marginale were in low concentration (below the cut off point, D.O. = 0.265 and ELISA levels, EL = 3), in the sera of buffalo, during the first 90 and 105 days, respectively for cows and calves. Then, the concentration of anti-A. marginale in the serum samples of buffalo calves, slightly raised to above the cut off point and kept in higher levels up to approximately 365 days after birth, indicating acquired immunity. Serum conversion for Babesia occurred in high levels and above the cut off point only for buffalo cows for all period of experimentation. The antibody levels against Babesia for both species and Anaplasma increased in the sera of buffalo cows between the days 91 and 335 after partum. In the colostrums, anti-B. bovis and anti-B. bigemina antibodies were detected in high levels during the first seven days after partum, but then abruptly declined to zero. Anti-A. marginale, on the other hand were not detected in the colostrums of these animals. When four animals (two buffalo cows and their calves) were individually analyzed it was observed an individual variation in the immune response: in one buffalo calf there was a strong passive... (Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:27:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-24Bitstream added on 2014-06-13T19:14:45Z : No. of bitstreams: 1 beraldo_lg_me_jabo.pdf: 244325 bytes, checksum: 15240886360c5cf0af075b2ef7715ab7 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Escherichia coli enteropatogênica (EPEC) e Escherichia coli shigatoxigênica (STEC) estão implicadas em causar sérias doenças no homem. Devido o crescimento da produção de búfalos leiteiros e a pequena quantidade de estudos sobre a prevalência de STEC e EPEC em bubalinos esse trabalho foi proposto. Os objetivos foram: determinar a prevalência de STEC e EPEC de amostras de origem bubalina através da pesquisa dos genes stx1, stx2, eae, iha, efa1, toxB, lpfAO157/OI-141, lpfAO157/OI-154, lpfAO113, saa, ehxA e bfp; caracterização bioquímica e sorogrupagem dos isolados e o perfil de resistência das estirpes de STEC e EPEC isoladas frente a diferentes drogas antimicrobianas. Foram isoladas 33 estirpes de E. coli, das quais 21 (63,6%) STEC e 12 (36,4%) EPEC, que apresentaram 23 perfis genéticos diferentes. Todos os isolados apresentaram mais de um gene de virulência. De todos os genes estudados apenas o bfp não foi encontrado nas amostras analisadas. As estirpes isoladas apresentaram sensibilidade a maioria dos antimicrobianos testados. O sorogrupo mais freqüente foi o O26, seguido do O157. As estirpes estudadas apresentaram genes de virulência que estão relacionados à graves doenças no homem. Pelos resultados pode-se dizer que búfalos são importantes reservatórios de STEC e EPEC e a presença desses isolados com determinados perfis genéticos devem ser melhor estudados / Escherichia coli (EPEC) and Shiga toxin Escherichia coli (STEC) are implicated in causing serious disease in humans. Due to the increased production of buffalo milk and small amount of studies on the prevalence of STEC and EPEC in buffalo this work was proposed. The objectives were to determine the prevalence of STEC and EPEC isolates from buffalo by investigating the genes stx1, stx2, eae, iha, efa1, toxB, lpfAO157/OI-141, lpfAO157/OI-154, lpfAO113, saa, ehxA and bfp; sorogrupagem and biochemical characterization of isolates and resistance profile of strains of EPEC and STEC isolates to different antimicrobial drugs. We isolated 33 strains of E. coli, of which 21 (63.6%) STEC and 12 (36.4%) EPEC, showed that 23 different genetic profiles. All isolates had more than one virulence gene. All of the genes studied only the bfp was not found in the samples. The strains isolated were sensitive to most antimicrobials tested. The serogroup O26 was the most common, followed by O157. The strains were virulence genes that are related to severe disease in humans. From the results we can say that buffaloes are major reservoirs of STEC and EPEC and the presence of strains with certain genetic profiles should be better studied

Orientador: André Mendes Jorge / Banca: Paulo Roberto de Lima Meirelles / Banca: Cristiana Andrighetto / Resumo: O presente estudo teve como objetivo analisar os dados de desempenho ponderal e curva de crescimento do rebanho bubalino Murrah da Faculdade de Medicina Veterinária e Zootecnia, da Universidade Estadual Paulista, Campus de Botucatu, no período de 1998 a 2009. Os pesos dos animais foram corrigidos às diversas idades padrão e o modelo estatístico incluiu os efeitos de sexo (S), mês (M) e ano de nascimento (A), classe de idade da búfala ao parto (C) e as interações S x M, S x A, S x C e M x A. As médias ajustadas e respectivos erros-padrão estimados para as características estudadas foram: PN: 37,89 ± 9,61kg; P120: 100,17 ± 20,28kg; P240: 170,36 ± 33,30kg; P365: 233,27 ± 35,57kg; P550: 309,85 ± 37,14kg e; P730: 395,14 ± 30,17kg. Houve efeito de sexo (S) somente para PN e P365, sendo que os machos tenderam a nascer mais pesados que fêmeas. O mês de nascimento (M) exerceu efeito sobre o PN, P120 e P730, sendo que animais nascidos em maio foram os mais pesados ao nascer, enquanto os nascidos em janeiro e maio foram os mais pesados aos 120 e 730 dias, respectivamente. O ano de nascimento (A) exerceu efeito sobre o PN, P120, P240 e P730. A classe de idade da búfala (C) exerceu efeito sobre o peso ao nascer, sendo que búfalas da classe 1 (3 anos ou menos) tenderam a parir bezerros mais leves e búfalas da classe 6 (10,11 e 12 anos) pariram bezerros mais pesados, o que contribui para a utilização desta característica na seleção das futuras matrizes do rebanho, respectivamente. O modelo linear foi o que melhor se ajustou aos dados para as curvas de crescimento / Abstract: This study aimed to analyze performance data and weight growth curve of Murrah buffalo herd of the Faculty of Veterinary Medicine, Universidade Estadual Paulista, Campus Botucatu, in the period 1998 to 2009. The weights of the animals were fixed for various ages and the standard statistical model included effects of sex (S), month (M) and year of birth (A), age class at calving buffalo (C) and the interactions S x M , S x, S x C x M and A. The adjusted means and their standard errors estimated for the characteristics studied were: NP: 37.89 ± 9.61 kg; P120, 100.17 ± 20.28 kg; P240, 170.36 ± 33.30 kg; P365, 233.27 ± 35.57 kg; P550, 309.85 ± 37.14 kg; and P730, 395.14 ± 30.17 kg. There was an effect of sex (S) only for BW and W365, and the males tended to be born heavier than females. The month of birth (M) exert effect on the PN, P120 and P730, and animals born in May were heavier at birth, while those born in January and May were heavier at 120 and 730 days, respectively. The year of birth (A) had an effect on the PN, P120, P240 and P730. The age class of buffalo (C) had an effect on birth weight, and buffaloes first class (three years or less) tended to give birth to lighter calves and buffaloes in Class 6 (10,11 and 12) gave birth to calves heavier, which contributes to the use of this feature in the selection of future headquarters of the herd, respectively. The linear model was the best fit to data for growth curves / Mestre

Resumo: Os búfalos possuem importância econômica como animais de produção no cenário agropecuário brasileiro e mundial. O conhecimento de polimorfismos em marcadores microssatélites mapeados em búfalos é imprescindível para auxiliar em questões evolutivas da espécie, variabilidade genética intra e inter-populacional, além de serem potencialmente úteis para os programas de melhoramento animal. Foram utilizadas três raças comerciais bubalinas, Mediterrâneo, Jafarabadi e Murrah de rebanhos brasileiros para análise de polimorfismos visando avaliar a diversidade genética existente dentro e entre as raças. Foram analisados 30 animais de cada raça pela amplificação do DNA genômico extraído do bulbo de pelos utilizando quatro locos microssatélites localizados em um cromossomo de interesse econômico. Os produtos de PCR foram separados por eletroforese vertical em gel de poliacrilamida desnaturante. O loco IDVGA-53 não obteve sucesso na amplificação e foi descartado das análises de polimorfismo. Os locos MB099 e BL41 foram monomórficos. Os parâmetros de diversidade foram gerados para o loco CSSM054, o único polimórfico, com média de 3,33 alelos por loco. A heterozigosidade observada com média de 0,503 foi maior que a esperada, indicando excesso de heterozigotos. As raças Mediterrâneo e Jafarabadi apresentaram desvio de equilíbrio de Hardy-Weinberg. O parâmetro Theta indicou evidências de que as três raças diferem entre si, e quando analisadas duas a duas foi possível verificar alta estruturação populacional entre elas. A maior distância genética foi verificada entre as raças Mediterrâneo e Murrah. O conteúdo de informação polimórfica revelou que o loco CSSM054 foi altamente informativo para a raça Mediterrâneo, portanto, considerando todos os resultados, essa raça apresentou maior variabilidade genética / Abstract: The buffalo water are economically important livestock in Brazilian global and agricultural scenario. The knowledge of polymorphisms in microsatellites markers mapped in buffaloes is essential to assist in evolutionary studies of species, genetic variability within and between populations, as well as being potentially useful for animal breeding programs. Three commercial breeds of buffalos (Mediterranean, Jaffarabadi and Murrah) of Brazilian herds were used for polymorphisms analysis to evaluate the genetic diversity within and among them. In each breed 30 animals were analysed by amplification of genomic DND extracted from the hair bulbs using four microsatellite loci located on a chromosome of economic interest. PCR products were separated by vertical electrophoresis in denaturing polyacrylamide gel. The IDVGA-53 locus was not successful in amplifying and was discarded from the analysis of polymorphism. The loci MB099 and CSSM054 were monomorphic. The parameters of diversity were generated for the locus CSSM054, the only polymorphic, with an average of 3.33 alleles per locus. With a mean value of 0.503, the observed heterozygosity was higher than the expected, indicating excess of heterozygotes. Mediterranean and Jaffarabadi breeds had deviation of Hardy-Weinberg equilibrium. The parameter Theta indicated evidence that the three breeds differ and when they were analyzed in pairs, it was observed high population differentiation among them. The largest genetic distance was found between the Mediterranean and Murrah breeds. The polymorphic information content revealed that the locus CSSM054 was highly informative to the Mediterranean breed, therefore considering all the results that race had a high genetic variability / Orientador: Vera Fernanda Martins Hossepian de Lima / Coorientador: Maria Elisabete Jorge Amaral / Banca: Sandra Aidar de Queiroz / Banca: Beatriz da Costa Aguiar Alves Reis / Mestre

The diagnostic performance of four serological assays for bovine brucellosis in African buffaloes, namely Rose-Bengal test (RBT), complement fixation test (CFT), indirect enzyme-linked immunosorbant assay (iELISA) and fluorescence polarisation assay (FPA) were evaluated and compared in a case-control study. The study followed the OIE assay validation pathway for validation of diagnostic tests applicable to wildlife species where there is a validated test available in a taxonomically closely related species. Two uninfected and four infected herds were recruited and an uninfected composite reference panel of 107 sera and infected composite reference panel of 93 were selected using composite reference standards. Diagnostic sensitivity (DSe) and diagnostic specificity (DSp) were calculated for individual tests and for different combinations of two tests in series and in parallel. Cut-off points were adjusted using receiver operating characteristics (ROC) analysis. Using these cut-off values, the index tests performed as follows: RBT DSe of 98.9% (95% CI 96.83% - 100%) and DSp of 98.1% (95% CI 95.6% - 100%), iELISA (cut-off >40.5%) DSe 98.9% (95% CI 94.2% - 100%) and DSp 100% (95% CI 96.6% - 100%), CFT (cut-off >0 iU/ml) DSe 74.2% (95% CI 64.1% - 82.7%) and DSp 100% (95% CI 96.6% - 100%) and FPA (cut-off >16 mP) DSe 97.9% (95% CI 94.2% - 99.7%) and DSp 100% (95% CI 96.6% - 100%). Based on performance index and area under the ROC curve, the iELISA performed best (198.9% and 1.0), followed closely by the FPA (197.9% and 0.989) and the RBT (197.0%). The CFT s lower performance (174.2%, and 0.871) was due to low DSe. Kappa values for test agreement between the index tests was above 0 for all combinations, and varied from unweighted Kappa of 0.685 (95% CI 0.608 0.762) between FPA and iELISA to 0.26 (0.136-0.383b) between CFT and RBT. Consideration of the indices for positive and negative test agreement between the index tests supported the differential specificity of tests for different immunoglobulin classes and higher in line with the findings in cattle. Positive predictive value in herd C and E were 100% for the iELISA, CFT and FPA, 97.3% in herd C and 98.4% in herd E for the RBT. Negative predictive values in herd C ranged from 89% for the CFT to 99.2% for the RBT and in herd E 73.1% for the CFT to 98.7% for the RBT. Overall repeatability was satisfactory, except for the FPA, which was considered the result of sample quality related to prolonged storage in a freezer. The index tests were all found fit for use to detect or confirm brucellosis in populations and individual animals. The values for DSe and DSp that were estimated will be of use in the interpretation of serological results and determination of diagnostic strategies in different circumstances. Different combinations of tests in series and parallel increased the DSp and DSe. Using the RBT in combination with the CFT/FPA/iELISA interpreted in series or in parallel in relation to the epidemiological setting and objective of testing is recommended. / Mini-dissertation (MSc)--University of Pretoria, 2015. / tm2016 / Veterinary Tropical Diseases / MSc / Unrestricted

The aim of this study was to test whether or not the equilibration time of two different cryodiluents influences the post thaw qualities of epididymal African buffalo (Syncerus caffer') sperm. Diluents and equilibration times were compared by assessing the post thaw spermatozoal motility, longevity and the acrosomal integrity. African buffaloes belong to Africa's "Big Five" and are, therefore, popular animals amongst game farmers, hunters and tourists. They are also asymptomatic carriers of foot-and-mouth-disease (FMD) and considered to be a wildlife reservoir for this plague. Other diseases, that are carried and can be transmitted from the African buffalo (Syncerus caffer') to livestock include tuberculosis, brucellosis and theileriosis or corridor disease (CD). Therefore, the transportation of African buffaloes is highly regulated. Disease-free buffalo populations are currently derived from a small genetic 8 pool and are smaller in their trophy size than the free-ranging animals from the diseased areas of the Kruger National Park (KNP) and the Hluhluwe/Umfolozi National Park. Hence there is a special interest in bringing new genetic material into the disease-free populations. Epididymal sperm from 11 mature African buffalo bulls was collected, diluted with two different semen extenders (TriiadylTM [Tris egg yolk extender] and AndroMed® [synthetic extender, i.e. fully defined medium]) and frozen. Pre-freezing equilibration times of 2 and 9 hours were tested. Total and progressive motilities, longevities and acrosomal integrity were measured and compared. Results show that there were no differences in post-thaw sperm quality when equilibration times between 2 and 9 hr were used. The use of the egg yolk containing extender (TriiadlyTM) resulted in higher percentage of post-thaw motilities than the use of the synthetic AndroMed®. Because a high percentage of progressive motile spermatozoa is one of the prerequisites for successful AI it must be concluded that TriladylTM is superior to AndroMed®. As I believe the advantages of higher motility to be bigger than the hygiene risks of a yolk containing extender I conclude that epididymal buffalo sperm should rather be frozen with TriiadylTM than with AndroMed®. / Dissertation (MSc (Production Animal Science))--University of Pretoria, 2003. / Production Animal Studies / unrestricted