Diploma thesis Business model of Internet platform and its implementation offers specific recommendations while establishing a business model of an Internet platform, based on social network Twitter. The first part focuses on theoretical approaches to platform creation, business model creation and strategy of foreign market penetration. In the second part steps for implementation of a specific platform- influento are analysed. The final part gives synthesis of knowledge aquiered in both theoretical and practical part and contains step by step recommendation for implementation. It suggests time and financial savings suggestions as well as alternatives to business model usage.

The aim of this thesis is to develop a business plan of fitness center with subsequent evaluation of the investment. First, the product itself and the ownership structure is introduced and than followed by an analysis of the market, customer, competitor analysis and analysis of suppliers, in which multicriteria decision with the help of Saaty method and the method of linear utility fiction is used. This is followed by personnel and marketing plan including marketing mix and marketing budget. In the financial plan, there is the opening balance sheet, statement of cash flow, profit and loss account for the first year and the balance sheet after the first year of business. In this section, the break-even point is calculated. The evaluation of the project is preceded by risk analysis. The sensitivity criteria of income is examined for the operation of two, or four factors. For the investment evaluation are used static and dynamic methods of evaluation. Under certain conditions, the project is profitable.

During the last decades, the circular economy has enabled a new kind of business model that is challenging the traditional linear way of doing business. The purpose of this thesis is to investigate how an international social enterprise work towards a circular business model, as well as the challenges and opportunities that comes with operating in an emerging market. Previous research on the subject is limited which leaves a research gap in existing literature. Indonesia is an emerging country that is struggling with a weak recycling infrastructure and   lack of waste management, which has resulted in an ocean plastic pollution problem.   In the theoretical framework, relevant aspects of the circular economy, social enterprises and opportunities and challenges with operating in an emerging market are presented. A conceptual framework concludes the literature review by illustrating how the concepts ties together. In order to answer the research questions, a qualitative research method has been followed throughout this single case study, combined with an abductive approach. The data has been collected through semi-structured interviews presented together with secondary data, in order to get a deeper understanding of the case. By examining an international social enterprise with operations in an emerging market, findings on what opportunities and challenges this social enterprise encounter in Indonesia are presented together with how they work towards a circular business model. This study indicates that the international social enterprise challenges the linear way of doing business and can be set as an example for other companies that has a social mission and wants to do business in a sustainable way.   The analysis discusses the relation between the theoretical concepts and the empirical findings, together with observations from the authors. The analysis is followed by the concluding chapter where the contributions are stated, and the research questions are answered, followed by implications, limitations and suggestions for future research. The findings showed that international social enterprises can find opportunities in infrastructure- and institutional gaps in emerging markets. In addition, the findings indicate that a strong social mission can reduce the challenges of operating in an emerging market. The findings have contributed to introducing the social enterprise phenomenon into the international business research field, as well as international social enterprises operating in emerging markets. The authors also provide their definition of the concept ‘social enterprise’.

Background: The football industry has been rapidly evolving. The value of transfer market has increased astronomically, thus sealing a transfer deal has become pricy. In the meantime, the gap between rich and low budget clubs keeps increasing. UEFA released new regulations called “Financial Fair Play” (FFP), which aims to curb financial differences amongst football clubs. Hence, football clubs adopted new entrepreneurial approaches such as scouting and youth academies to improve their financial situation. Purpose: The main objective of this paper is to understand football teams’ entrepreneurial reaction towards FFP. The purpose is to examine the strategies of value creation from human capital like talented young players, in terms of scouting and youth academy, and explain the relationship between football and entrepreneurship in context of the business model framework. Method: The research is conducted by secondary data collection through online resources. We used the secondary data analysis method to analyze our data and carried out a representative case study based on the theoretical framework of this master thesis on two football clubs, AFC Ajax and Borussia Dortmund, to understand the problem more comprehensively. Conclusion: The results of this study demonstrate that the FFP regulations affected the entrepreneurial structure of football clubs. The data suggest that both clubs intensified their scouting and youth academy activities, as a result they generated more transfer revenue through selling the players that they recruited at their youth academies or scouted. Hence, this new entrepreneurial approach that the football clubs adopted provided a significant benefit for them. We call this concept that football meets entrepreneurship as “Footballpreneurship”.

Healthcare associated infections (HAIs) cost the US healthcare system over $45 billion to treat and cause millions of deaths annually. A large subset of HAIs are associated with medical devices that are meant to improve and save lives. Infected devices are treated using traditional antibiotics, contributing to development of antibiotic resistance. Antibiotic resistance is expected to cost $100 trillion and kill more people a year than cancer by 2050; thus, new alternative antimicrobials for the treatment of device-associated HAIs are critically needed. Antimicrobial peptides (AMPs), such as 26 amino-acid (aa), marine-derived Chrysophsin-1 (CHY1), are poised to reduce HAIs due to their broad antimicrobial activity and unique mechanisms of action that do not promote bacterial resistance. AMPs are short (12-50aa), positively charged (+2-+9) proteins found in the innate immune systems of many different species. Their high separation of hydrophilic and hydrophobic residues leads to many unique mechanisms derived from many unique secondary and tertiary structures that are not yet well understood. Despite the discovery of over 2000 natural AMPs and many more synthetically designed AMPs, none have been successfully commercialized for healthcare applications due to challenges surrounding cytotoxicity, short in vivo half-life (degradation), high costs of production and effectiveness in physiological environments (such as those with high-salt). Several strategies have been investigated to overcome these challenges, for example, truncation of cytotoxic sequences or D-amino acid substitution to improve AMP toxicity and stability; however, many of these strategies can reduce antimicrobial effectiveness. A unique strategy of increasing stability, reducing cytotoxicity, and maintaining antimicrobial activity that is relevant for medical devices is the covalent tethering (binding) of AMPs via a flexible tethering molecule to the surface. However, the effect of tethering parameters on resulting AMP mechanisms and activity is still widely debated. AMP activity can vary widely by utilizing different tethering strategies, which include additional variables such as: (1) peptide choice and properties (such as native mechanism, concentration, charge, and structure), (2) tether choice and properties (such as chemical composition, length, charge, surface density, and flexibility), and (3) testing conditions (such as temperature, solvent composition and substrate type). Some studies suggest that AMP performance may be tether-dependent, for example some AMPs require longer tethers while others do not and some need a flexible tether. Thus, models for predicting successful tethering strategies for different AMP properties, which currently do not exist, must be developed. Further, complicated and often destructive techniques, such as XPS and SEM, are typically implemented to study the relationship of all these parameters vs. antimicrobial activity, which are labor-intensive and limited in scope. Predictive models guiding tether strategy need to be constructed, but also new techniques to study tethering be developed. If these technical milestones are achieved they can serve as a predicate for commercial implementation of a host of new therapies targeted at reducing device-associated HAIs. The overall goal of this thesis was to study the relationship between antimicrobial activity of tethered C-CHY1 examining both spacer length and peptide surface density and the development of a feasible clinical business case for tethered AMPs. To achieve this goal, a traditional entrepreneurial approach was taken in which a minimally-viable product was first designed and business case analyzed, followed by studies to better optimize and understand the underlying structure-mechanism relationships. CHY1 with a C-terminus cysteine to allow for surface-binding (C-CHY1) was tethered onto a silicon dioxide surface via a flexible poly(ethylene glycol) (PEG) tether, and then both surface binding behavior and antimicrobial success of C-CHY1 were examined as a function of tether properties and reaction conditions. For these studies, quartz-crystal microbalance with dissipation (QCM-D) was the primary technique, a real-time, non-destructive flow method that was then coupled with downstream characterization techniques: fluorescent microscopy and contact angle measurements. In parallel a deep dive into domestic and international business models for commercializing AMP technologies. Specifically, tether length and surface density effects on C-CHY1 mechanisms were studied, followed by the effect of temperature, type of microbe, and salt concentration on the antimicrobial mechanisms of tethered C-CHY1. QCM-D was used to measure binding of C-CHY1 via three different length tethers, PEG molecular weight (MW) 866, 2000 and 7500, followed by microscopy to measure antimicrobial effectiveness against two model microbes Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Modeling of QCM-D data allowed for surface density and thickness to be calculated and related to C-CHY1 antimicrobial activity. PEG 7500 allowed proper C-CHY1 orientation and mobility, allowing for its native pore-forming mechanism and highest activity while PEG 866 tethers led to denser grafting and an effective, yet non-native ion displacement mechanism. The QCM-D was used to characterize the effect of salt concentration and temperature reaction conditions on the grafting density of C-CHY1 tethered via PEG 866 and PEG 7500, which was then related to antimicrobial activity. For PEG MW 866, neither temperature nor salt concentration increases significantly altered the grafting density of C-CHY1 while for PEG 7500 increasing temperature allowed for significantly increased grafting density. C-CHY1 density had no significant effect on antimicrobial activity against either microbe. Temperature of bacterial incubation did demonstrate microbe-specific changes in C-CHY1 antimicrobial activity. These results demonstrated that small changes in reaction conditions can drastically change membrane selectivity of C-CHY1. An in-depth investigation of the effects of bacterial membrane composition and temperature on soluble C-CHY1 mechanism was implemented to better understand the molecular membrane- and temperature-dependent selectivity and structure-function of C-CHY1. Supported lipid bilayers (SLBs) formed in QCM-D can be used as model membranes to elucidate AMP action mechanisms against membranes of different compositions. Two and three component SLBs representative of Gram-negative phosphatidylethanolamine (PE) and phosphatidyglycerol acid (PG) with and without charged lipopolysaccharide, LPS and Gram-positive bacteria phosphatidylcholine (PC) and PG with and without charged lipoteichoic acid, (LTA) were formed at both 23°C and 37°C. C-CHY1 at 5 µM was exposed to the different membranes and mechanistic surface action was studied. The membranes formed highly different baseline responses in QCM-D, indicative of vastly different membrane structures, thicknesses and deposition behaviors on SiO2, warranting future studies. Further, significant effects of LTA incorporation were observed in both peptide interaction and deposition. There were measurable effects of temperature on membrane formation as well as peptide interaction kinetics and even mode of interaction. Lastly, business models for the commercialization of novel medical device technologies such as surface-tethered C-CHY1 were investigated. While this technology has the potential to solve many unmet needs, there must a commercialization plan implemented in order to have an impact. There is a clear disconnect between technology development in academia and technology commercialization in industry that must be connected. Development of an entrepreneurial mindset at the graduate school level, can help bridge the gap. A thorough investigation of domestic and international business models for commercializing AMP technologies was carried out and distilled in the form of the Business Model Canvas developed by Alexander Osterwalder that can be used as a roadmap for commercialization efforts. Using the QCM-D a relationship between both spacer length and peptide surface density and the antimicrobial activity of tethered C-CHY1 was determined. A business plan was developed in order to increase the impact of this and other AMP based work. This work provides a roadmap for future researchers to quickly develop and commercial novel AMP based coating technology.

Stakeholder interaction has been recognised as one of the main principles to advance organisational sustainability. However, there is only limited knowledge on how organisations interact with their stakeholders to develop sustainability practices from an overarching perspective. There is also only fragmented research on the role of stakeholders when developing a sustainable business model (SBM). The purpose of this thesis is therefore to explore the role of stakeholder interaction for developing organisational sustainability practices and SBMs. Three research questions drive this thesis which compiles three papers based on three individual studies. The first study concentrates on stakeholder interaction as an organisational sustainability practice applying quantitative methods. The second study is a systematic literature review analysing the role of stakeholder interaction to develop SBMs. The third study explores how organisations implemented stakeholder interaction efforts, using qualitative methods. The results reveal that stakeholder interaction implemented as organisational sustainability practices and part of the SBM includes multiple stakeholders and different interaction degrees, potentially leading to new value opportunities and virtuous cycles of sustainable value creation. However, while organisations recognise the importance of stakeholder interaction, most organisations focus on low-to medium interaction degrees related to specific sustainability activities. This may limits the potential of stakeholder interaction for developing organisational sustainability practices and SBMs. This thesis contributes to sustainability, stakeholder and SBM literature by systematically exploring the role of stakeholder interaction, highlighting different degrees and extents practices are implemented in organisations. For practitioners, insights are offered how to foster stakeholder interaction practices and with that move towards organisational sustainability.

The diploma thesis is focused on the development of business activities of the company AFEED, a. s., which deals with the production of feed for farm animals. First, the theoretical basis is developed, which serves as a basis for the analysis of the internal and external environment. The results of the analysis are summarized in the SWOT analysis. Based on the results, a proposal for the development of business activities is prepared.

The main goal of this thesis is the creation of business plan for establishing a small company. It's an internet shop selling the equipment for american football. In the first part, I'm going to focus on relative teoretical concepts, perceptions and structure of the business plan. In the following part, I'm going to analyze internal and external environment. And based on the resaults, the last and final part will contains the original design of the business plan.

This diploma thesis deals with the creation of a business plan. This plan is concerned with the startup of an online store which specializes in speakers, headphones and accessories. The thesis explores business plan theory and utilizes the SLEPTE, SWOT and McKinsey 7S analyses complemented by an analysis of my own research. Based on the above mentioned information, the business plan itself is drawn up.

The dissertation deals with the issue of novel business models based on sustainable multiple customer value creation. The issue is evaluated from the perspective of novel approaches to business models integrating environmental, social, and economic value into corporate activities. The dissertation’s objective is creating novel business model concepts based on sustainable multiple value creation for the Czech chemical industry, namely category CZ_NACE 20.1. Primary and secondary research was conducted in the making of dissertation. Research results are presented as partial conculussions describing individual areas, as well as a general conclussion. For easier interpretation of results, the disseratation emphasizes visual interpretations of provided novel business model ceoncepts. The conclussion provides contributions for theory and research, practictioners and teaching practice.