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Data for this study were 79,997 evaluations for 12 individual body and udder traits of Holstein cows. Type scores were subjectively assigned by trained personnel of Select Sires, Inc., Plain City, OH, through their corrective mating and young sire evaluation programs. After editing data to remove incomplete, erroneous and duplicate records, 56,642 records were used to calculate overall and regional age adjustment factors. Genetic parameters were estimated. Heritabilities ranged from .40 for stature to .14 for fore udder. Standard errors ranged from .039 for stature to .018 for fore udder. Genetic correlations ranged from +.75 between teats and udder support to -.61 between feet and legs. Phenotypic correlations were generally smaller than genetic correlations, ranging from .56 between udder support and teats to -.23 for dairyness and strength. These findings were in general agreement with previous research. Genetic evaluations for the 12 individual linear traits were made using Herdmate Comparison (HC) and Best Linear Unbiased Prediction (BLUP) methods. Mean evaluations by HC were near zero, and ranged from .026 for stature to -.027 for teats. Mean repeatability also showed very little variation, ranging from .404 for stature to .241 for teats. BLUP evaluations were similar to HC evaluations, with mean evaluation ranging from .121 for feet to -.183 for dairyness. Direct correlations between BLUP and HC evaluations ranged from .90 for stature to .74 for dairyness. Forty-eight different combinations of minimum daughter numbers and model variables were tested to predict sires' overall evaluations for type from linear trait evaluations using regression analysis. Results indicate that with 5 or more daughters per sire, all predictions tested were similar in accuracy for Predicted Difference for Type. / M.S.

Stearoyl-CoA desaturase1 (SCD1) catalyzes the synthesis of conjugated linoleic acid (CLA) and mono-unsaturated fatty acids (MUFA) in the mammary gland of ruminant animals. We hypothesized that single nucleotide polymorphisms (SNPs) in the coding region, 5' and 3' untranslted regions (UTRs) of the SCD1 gene would influence the activity of SCD1 enzyme and consequently account for some within-breed variations in milk CLA and MUFA. Sequence analysis of the coding region of the SCD1 gene of Jerseys and Holsteins revealed c.702A→G, c.762T→C and c.878C→T SNPs in exon 5 in both breeds and c.435G→A in exon 3 in Holsteins. The SNPs resulted in: A (G435A702T 762C878), A1 (A435A702T 762C878), B (G435G702C 762T878) and B1 (A435G702C 762T878) coding variants in Holsteins and only variants A and B in Jerseys. Only SNP 878C→T resulted in a non-synonymous codon change resulting in p.293Ala and p.293Val protein variants or alleles at the SCD1 locus. Subsequent association studies found significantly higher C10 index, C12 index and C14 index and consequently higher concentrations of C10:1 and C12:1 in p.293AA cows compared to the p.293VV cows in both breeds. The SCD1 genotype had no influence on concentrations of C141, C16:1, C18:1 and CLA in both breeds. / Sequence analysis of the 5' and 3' UTRs revealed no SNPs in the 5'UTR and a total of 14 SNPs in the 3'UTR of both breeds. The SNPs were in complete linkage disequilibrium resulting in 3 haplotypes or regulatory variants: H1 (G1571G1644C1763C2053A2584 A3007C3107G3208 T3290G 3497G3682A4399C4533G4881), H2 (G1571G1644A1763C2053A 2584G3007 C3107G3208T3290G3497G 3682A4399C4533G4881) and H3 (T 1571C1644A1763 T2053G2584G3007T 3107A3208C3290A3497A3682T 4399T4533A4881) in Holsteins and only H1 and H3 variants in Jerseys. A subsequent association study involving 862 Holstein cows, found the H1 regulatory variant to be associated with higher C10 and C12 desaturase indices and consequently with higher concentrations of C10:1 and C12:1 compared with the H3 variant. The effects of the H2 variant were intermediate to those of H1 and H3. 3'UTR genotype had no influence on the concentrations of C14:1, C16:1, C18:1 and CLA. The concentrations of C10:1 and C12:1 in milk fat could therefore be due to effects of SNPs in the open reading frame and the 3'UTR regions of the SCD1 gene. These results indicate that SNPs in the coding and 3'UTR regions of the SCD1 gene could be used as markers for genetic selection for increased C10:1 and C12:1 contents of milk.

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