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Vision studies and visual acuity investigations are an ongoing and progressive field in veterinary ophthalmology. These independent studies all help to contribute to a combined and collective knowledge in our understanding of this truly complex matter. Understanding retinal morphology and physiology is an integral factor in piecing together overall function of the eye. Many of these studies have been done in both medical and veterinary ophthalmology using behavioural factors, electrophysiology, special staining and scanning techniques on a histological level. In the veterinary field many species have been studied pointing out similarities or differences among them. This study hopes to contribute to the understanding of the retinal ultrastructure of the African buffalo (Syncerus caffer). Twenty-five pairs of African buffalo eyes were obtained, but only forty-eight eyes were included in this investigation. The globes of one donor appeared to have chronic intraocular disease and were phthisical. Since this is a descriptive study of normal anatomy and function, these eyes were excluded. Globe dimensions were recorded and statistically analysed, revealing an average horizontal diameter of 32.91mm and a vertical diameter of 33.04mm. The median age of the donor group was 4 years with Using scanning electron microscopy it was established that African buffalo retinas, like other domestic species, have a specialised region a few millimetres dorsolateral to the optic disc, synonymous to the well described area centralis. In this region a higher concentration of cones is found as opposed to other rod-rich regions. In a concurrent investigation, the contralateral globes were processed for immunohistochemical antibody staining. Colour specific anti-bodies were used to identify the cone population present in the African buffalo retina. The conclusion of this investigation reveals that this species like other domestic animals has dichromatic colour vision, recognising short and medium to long colour wavelengths. / Dissertation (MMedVet)--University of Pretoria, 2013. / gm2014 / Companion Animal Clinical Studies / unrestricted

Self-calibrating eye tracker using image saliency. / Självkalibrerande ögonspårare med hjälp av image saliency.

No / PURPOSE: To examine foveal structure in amblyopia using spectral-domain optical coherence tomography (SD-OCT). DESIGN: Prospective, cross-sectional study. PARTICIPANTS AND CONTROLS: Two subject groups were recruited to the study: 85 amblyopes (34 adults, 51 children) and 110 visually normal controls (44 adults, 66 children). METHODS: A detailed eye examination, including an SD-OCT scan, was performed in all participants. A total of 390 eyes of 195 subjects were imaged using a 3-dimensional (3D) macula scan covering a nominal 6 x 6-mm area with a resolution of 256 x 256 (65,536 axial scans). Data from the B-scans bisecting the fovea both horizontally and vertically were fitted with a mathematical model of the fovea to determine a range of foveal parameters. MAIN OUTCOME MEASURES: Foveal thickness, foveal pit depth, and foveal pit slope. RESULTS: Bilateral differences between the eyes of amblyopes compared with visually normal controls were found. The difference between foveal structure in amblyopic participants relative to structure in subjects with normal vision persisted even when variables such as age, ethnicity, axial length, and sex were taken into account. Amblyopes showed increased foveal thickness (+8.31 mum; P = 0.006) and a reduction in pit depth in the horizontal meridian (-10.06 mum; P = 0.005) but not in the vertical meridian (P = 0.082) when compared with subjects with normal vision. Foveal pit slopes were found to be approximately 1 degree flatter in the nasal (P = 0.033) and temporal (P = 0.014) meridians in amblyopes, but differences between amblyopes and controls in the superior (P = 0.061) and inferior (P = 0.087) meridians did not reach statistical significance. No statistically significant interocular differences were found in the foveal structure between amblyopic and fellow eyes. CONCLUSIONS: Differences were found in the foveal structure in both eyes of amblyopes compared with subjects with normal vision. These differences consisted of increased foveal thickness, reduced pit depth when measured along the horizontal meridian, and flattening of the nasal and temporal sides of the foveal pit.