The thesis analyzes the issue of securing the airways in special conditions. The author is a military anesthesiologist, so the special conditions in this work are understood as the situation on the battlefield. The introductory part of the thesis discusses the historical development of the view of airway management, describes the various methods of airway management and their position in "combat airway management". The research part of the thesis first explores supraglottic airway management techniques, which in today's concept of algorithms and recommendations is intended primarily for non-medical professionals and/or health professionals who do not have much experience with complex methods of airway management. The first part has shown that the use of supraglottic devices by inexperienced rescuers is safe and that it is possible to define and choose from a large number of aids that are the most suitable on the market. The second part of the research part was focused on tracheal intubation and portable video laryngoscopes. It has shown that these can improve intubation conditions even if performed by an inexperienced rescuer. The main research effort has been focused on the most critical part of airway management procedures, surgical airway access. A live large laboratory animal proved to be the...

ABSTRACT This work is focused on the synthesis of a family of new macrocyclic ligands with exchangeable protons on coordinating groups that could potentially serve (after complexation with suitable paramagnetic lanthanide(III) ions) as responsive contrast agents (CAs) for magnetic resonance imaging (MRI). It is expected that measurement of extracellular pH should bring information for tumorous disease diagnoses and/or for suggesting the most efficient treatment. Therefore, our attention was focused on pH-dependent CAs based on a PARAmagnetic Chemical Exchange Saturation Transfer (PARACEST) mechanism capable of reporting pH changes in tissue. The PARACEST-related properties of a series of Ln(III) complexes with the CEST effect caused by amino groups coordinated to the central Ln(III) metal ions were investigated. Such a kind of PARACEST CA is new and has had no precedent in the literature. It was shown that these Ln(III) complexes produce a pH-sensitive PARACEST effect in the pH region relevant for living systems. The study brings proof-of-principle for utilization of complexes with a linear diamine pendant arm, i.e. complexes with two exchanging proton pools, for ratiometric pH determination by MRI independently on the probe concentration. In addition, to ensure a higher kinetic inertness of the...

This diploma thesis describes searching for long forgotten routes between ancient settlements in the mountainous environment of Central Bulgaria. It focuses on Stara Planina Mountains, where the highest peaks over two thousand metres are. Geographical features of this area were analysed using three-dimensional tools in GIS environment. I experimented with various route-designing tools and tested their capabilities against well-known ancient paths. The most accurate tools were then selected to predict routes on maps of ancient settlements from various historical periods. The data used to construct these maps had been collected from two different sources. The first source was a multidisciplinary project called TRAP, initiated by Mgr. Adéla Sobotková, the consultant of my thesis. This project's data was collected during spring and autumn surface survey campaigns in the Kazanluk region of Tundzha River valley. The second source was specialized monographs and journal articles about settlement and path connections in ancient Thracia in the period between 6th century BC and 4th century AD.

Abstract The present thesis exploits advanced liquid and gas NMR methods for the characterization of various interesting materials. The methods used to study the structural properties of thermally modified wood, ionic liquids, cements, shales, and porous organic cages include MRI, NMR cryoporometry, Laplace NMR, multidimensional Laplace NMR, as well as ¹²⁹Xe and ¹⁹F NMR. The commonality factor in all the studies is the usage of either inherent or introduced liquid or gas molecules to probe the topical materials. The MRI method was utilized to visualize the water absorption phenomena in the thermally modified pine wood. High-resolution images made it possible to observe the spatial distribution of free water and the changes in the rate of absorption of water in wood samples modified at different temperatures. The images also helped to resolve the individual resin channels. T₂ maps enabled us to observe the changes in the relaxation values of free water in thermally modified wood as compared to their unmodified reference wood samples. The multidimensional Laplace NMR methods were exploited to study the structural and dynamical properties of a novel halogen-free, boron-based ionic liquid (hf-BIL). NMR self-diffusion (D) experiments showed the presence of two coexisting dynamic phases in hf-BIL. Multidimensional D − T₂ correlation experiments made it possible to determine the T₂ relaxation times of the slow and fast diffusing phases. T₂ − T₂ relaxation exchange measurements allowed quantifying the exchange rates of anions and cations between the phases. Moreover, the theoretical modeling of the experimental data revealed that the slow diffusing phase was composed of anion-cation aggregates, while the fast diffusing phase was comprised of free anions and cations. ¹²⁹Xe NMR analysis of the xenon adsorbed in the cements and shales helped us to determine their porous structures. The method exploits the high sensitivity of the chemical shift of ¹²⁹Xe to its local environment. The chemical shift value of ¹²⁹Xe enabled us to estimate the size of the mesopores in the cement samples. The exchange spectroscopy (EXSY) measurements were used to determine the exchange rates between the free gas and mesopores of the cement samples. ¹²⁹Xe NMR spectra of the shale samples provided information about pore sizes and paramagnetic compounds. ¹H NMR cryoporometry measurements of the shale samples immersed in acetonitrile made it possible to analyze the pore size distribution ranging from 10 to over 100 nm. Moreover, T₂ − T₂ exchange measurements helped us to quantify the exchange rates of acetonitrile in the shale samples. Xenon and SF₆ were used as internal reporters to gain versatile information on adsorption phenomena in the cage and window cavities of the crystalline porous organic cages. ¹²⁹Xe NMR analysis of the adsorbed xenon helped us to determine the diffusion coefficients and activation energy of diffusion as well as thermodynamic parameters. With the help of T₂ relaxation time values, it was possible to estimate the exchange rates between cage and window cavities. Chemical exchange saturation transfer (CEST) experiments resolved a window cavity site, which arises from crystal defects in porous organic cages. In addition, ¹⁹F NMR analysis made it possible to estimate the relaxation rates and diffusion coefficients of SF₆ gas in porous organic cages. Modelling of the T₁, T₂ and diffusion data confirmed that the cage to window exchange is the completely dominating mechanism for ¹²⁹Xe T₂ relaxation. T₁ relaxation is dominated by diffusion modulated dipole-dipole relaxation (DDinter) and chemical shift anisotropy (CSA) relaxation due to local cavity mobility. Whereas, in case of SF₆ T₂ data, the dominating mechanism is diffusion modulated dipole-dipole relaxation and for T₁ the local tumbling of SF₆ in cage cavity is the key dynamics behind the dipole-dipole and CSA mechanisms. / Original papers The original publications are not included in the electronic version of the dissertation. Javed, M. A., Kekkonen, P. M., Ahola, S., & Telkki, V.-V. (2015). Magnetic resonance imaging study of water absorption in thermally modified pine wood. Holzforschung, 69(7), 899–907. https://doi.org/10.1515/hf-2014-0183 Javed, M. A., Ahola, S., Håkansson, P., Mankinen, O., Aslam, M. K., Filippov, A., … Telkki, V.-V. (2017). Structure and dynamics elucidation of ionic liquids using multidimensional Laplace NMR. Chem. Commun., 53(80), 11056–11059. https://doi.org/10.1039/c7cc05493a http://jultika.oulu.fi/Record/nbnfi-fe2017102750335 Javed, M. A., Komulainen, S., Daigle, H., Zhang, B., Vaara, J., Zhou, B., & Telkki, V.-V. (2019). Determination of pore structures and dynamics of fluids in hydrated cements and natural shales by various ¹H and ¹²⁹Xe NMR methods. Microporous and Mesoporous Materials, 281, 66–74. https://doi.org/10.1016/j.micromeso.2019.02.034 http://jultika.oulu.fi/Record/nbnfi-fe2019041712678 Komulainen, S., Roukala, J., Zhivonitko, V. V., Javed, M. A., Chen, L., Holden, D., … Telkki, V.-V. (2017). Inside information on xenon adsorption in porous organic cages by NMR. Chemical Science, 8(8), 5721–5727. https://doi.org/10.1039/C7SC01990D http://jultika.oulu.fi/Record/nbnfi-fe201709288804 Håkansson, P., Javed, M. A., Komulainen, S., Chen, L., Holden, D., Hasell, T., … Telkki, V.-V. (2019). NMR relaxation and modelling study of the dynamics of SF₆ and Xe in porous organic cages. Manuscript.

Respiratory diseases are ones of the most common diseases, which are treated by general practitioners. Not only the right diagnosis is essential, but the treatment as well. The treatment of respiratory diseases is usually long-term and often requires repeating. Health treatment of young children in a spa improves their life and health quality, inclusion in a collective of similar aged children, reduces complications of their disease, doses of medicine and highly reduces the risk of development of a chronical disease in adulthood. The aim of this thesis is to chart the use of coordinated rehabilitation in spa Lázně Luhačovice, a. s. and to survey coordinated rehabilitation awareness of spa medical and non medical personnel and children's accompaniment. Three hypotheses were set. Hypothesis 1: Children's accompaniment, who has been to spa health resort repeatedly percieves the care of his child as coordinated unlike the one who has been to spa health resort just once. Hypothesis 2: Medical staff of children spa health resorts percieves the most the medical treatment part of coordinated rehabilitation in contrast with non medical staff. Hypothesis 3: Children's accompaniment is satisfied the most with the medical treatment part of coordinated rehabilitation unlike the pedagogical and social one. A strategy of quantitative research was used, data was gathered by standardized questionnaires in June and July 2015. The standardized questionnaires were given to two different research groups. First one was spa health resort personnel, second one children's accompaniment in the spa Lázně Luhačovice, a. s. Based on statistical calculations the hypotheses can not be rejected, the results are not statistically significant. From the data obtained from the questionnaires, it is noticable that from the spa health resort medical and non medical personnel point of view, there is percieved only the part, which they provide. On the other hand, from the children's accompaniment point of view the rehabilitation is percieved as coordinated in all the parts provided. It is thanks to the fact that the accompaniment, who is in the spa health resort on the expenses of health insurance company, can see all the parts of coordinated rehabilitation as they undergo it together with their children. This thesis contributes to widening awareness of using parts of coordinated rehabilitation in children repeated respiratory diseases treatment, which are provided in facilities of spa Lázně Luhačovice, a.s. and in other health resorts, where there is this kind of care provided as well.

Clefts of orofacial komplex are the most common birth development defects. One of them is Pierre Robin sequence, which is characterized by presence of micrognathia, glossoptosis and upper airway obstruction. Because of these symptoms, isolated cleft palate "U" or "V" shaped can be part of the diagnose. Other clinical manifestations are feeding difficulties, obstructive sleep apnea syndrome and / or gastroesophageal reflux disease. The aim of the treatment is to secure the airway and the child's thriving after birth by using conservative or invasive treatment methods. Choosing of methods and procedures should be established by multidisciplinary cooperation. The other part of diploma thesis is trying to find out, which clinical manifestation are the most common in patients from the Czech Republic compare to foreign patients and the differences in management of the treatment. Furthermore we are trying to investigate, whether there is higher percentage of patients with delayed neuromotor development among PRS patients, which are under physical therapy. The last part is trying to find out the relationship between the poor posture and PRS diagnose in children age 4 - 6 years.

Introduction: Intraperitoneal tumor seeding is a common form of recurrence after surgery for GI tumours. Early diagnosis and treatment may influence the course of disease. Hypothesis and objectives of the work The finding of free tumor cells and / or elevation of tumor markers in peritoneal fluid predicts intraperitoneal tumor recurrence. The results of these tests can be used to indicate HIPEC. Aim of this work is to determine the sensitivity and specificity of cytology and tumor markers levels and importance of these tests for early diagnosis and prognosis of recurrence. Another objective was to confirm the applicability of RT PCR for identification of free tumor cells. Patients and methods . Materials are sourced both during initial surgery (220 patients) and by the dispensary controls (25 patients). The peritoneal fluid was examined cytologically and biochemically too. 50 samples was exemined by RT PCR. Results Elevation of tumor markers or finding of free intraperitoneal tumor cells predicts recurrence. The prognosis of these patients corresponds to stage IV TNM classification, regardless of other parameters TNM. Low sensitivity of these tests (34% for cytology, 53% for tumor markers) does not allow its use as an indicator of HIPEC. Due to degradation of RNA we did not confirm the value of RT...

The main objective of the thesis is to explain and advocate the topic of career paths and construct career paths for technical professionals of the selected company. The thesis then has several secondary objectives.This thesis is divided into two parts. The first part provides a theoretical framework for career paths in terms of getting into the topic of career paths and explaining the purpose, and furthermore, provides a guide for career path framework construction. The second, practical part focuses on the selected technological company that wishes to remain anonymous. Based on the theory and partially extracted from the interviews with the company experts, the career paths framework for technical professionals is constructed. To ensure that the framework is valuable for the company, feedback for the proposed framework is gathered and implemented in the thesis.

The orienteering problem is one of the newer problems in the field of discrete programming. The formulation of this problem originates from a sport discipline, called orienteering. In the beginning of this thesis a formulation and mathematical model for this problem are introduced. The extension of the problem is the team orienteering problem, described in the next chapters of theoretical part. Many heuristics were published for this problem. While the heuristic of Chao et al. and a path relinking approach are described in detail in this thesis. Practical part deals with the team orienteering problem applied to a real task, specifically a visiting of 23 attractions in the New York in different number of days. The solution is found by optimization program Lingo 90 and by heuristic of Chao el al. Heuristic algorithm was implemented in programming language Visual Basic for Application. A comparison of the results is described at the end of the practical part.

The aim of this thesis was to implement a software that would be able to render, simulate and record a scene with walking pedestrians in real-time, with emphasis on rendering level of realism. The output of the application could serve as an input test data for people counting systems or similar systems for video recognition. The problem was divided into three major subproblems: character animation, artificial intelligence for character movement and advanced rendering techniques. The character animation problem is solved by the skeletal animation of the model. To achieve the characters moving in a scene autonomously path finding(A* algorithm) and group behaviors(steering behaviors) were implemented. Realism in a scene is added by implemented methods such as normal-mapping, variance shadow-mapping, deffered rendering, skydome, lens flare effect and screen space ambient occlusion. Optimaliaztion of the rendering was implemented using octree data structure for space partitioning. Rendering stage of a scene can be easily parametrized through implemented GUI. Implemented application provides the user with easy way of setting a scene with walking pedestrians, setting its visualization and to record the result.