This thesis presents the characterization process of an imaging device for a satellite. The camera system was built by the Space Dynamics Laboratory (SDL) and will be used in the Michelson Interferometer for Global High-resolution Thermospheric Imaging (MIGHTI) instrument for National Aeronautics and Space Administration's (NASA) Ionospheric Con- nection Explorer (ICON) satellite. This mission will further scientists' understanding of the connection between the Earth's weather and ionospheric conditions. The ionosphere, a part of the atmosphere, interferes with satellite communications, causing disturbances and disruptions. By learning more about the ionosphere through the data collected by this instrument, scientists will better understand its effects on our communications.

La tomographie optique diffuse (TOD) est une technique d’imagerie médicale relativement récente qui utilise la lumière dans le proche infrarouge pour acquérir des images in vivo de façon non invasive. Cette technique est en utilisation croissante par de nombreux chercheurs et biologistes et plusieurs équipes dans le monde travaillent sur le développement de scanners par TOD y compris notre groupe de recherche (groupe TomOptUS). Le Centre d’imagerie moléculaire de Sherbrooke dispose d’un appareil pour imagerie optique sur petit animal développé par la compagnie Quidd, soit le QOS (Quidd Optical imaging System). Cet appareil est utilisé par des biologistes et chercheurs pour diverses études précliniques sur modèles animaux (souris) de maladies humaines comme le cancer. Le QOS est entièrement contrôlé par ordinateur à l’aide d’un logiciel sophistiqué (le QOSoft) qui permet d’obtenir des images en fluorescence et en bioluminescence. Il est toutefois limité en ne permettant d’acquérir que des images planaires de la lumière sortant d’un animal ; il ne permet pas la tomographie, à savoir obtenir des images tridimensionnelles (3D) des sources fluorescentes ou bioluminescentes situées en profondeur à l’intérieur de l’animal. Bien que le QOS offre une grande flexibilité en terme d’angle d’acquisition d’images autour de l’animal avec sa caméra montée sur un bras rotatif, il a une sensibilité limitée pour de l’imagerie en profondeur, notamment parce qu’il fonctionne en mode épiillumination (détection de la lumière du même côté que l’injection de la lumière excitatrice dans l’animal) et aussi à cause de la sensibilité limitée de sa caméra. Afin d’augmenter les capacités tomographiques et la sensibilité du QOS, ainsi que le contraste des images qu’il fournit, le présent projet propose des développements logiciels intégrés au QOSoft. Ces ajouts logiciels au niveau du contrôle d’instrumentation et de l’interface graphique permettent d’intégrer une caméra EMCCD à ultra-haute sensibilité et ultra-faible bruit pour remplacer la caméra CCD refroidie existante ainsi qu’un module d’illumination laser rotatif. Ce module d’illumination, développé par le groupe TomOptUS, permet l’imagerie en mode transillumination ainsi que toutes les configurations intermédiaires jusqu’à l’épi-illumination. Ce module permet en outre d’injecter une densité de puissance lumineuse supérieure à celle possible avec la configuration actuelle du QOS. Le QOS et son logiciel mis à jour avec les ajouts faisant l’objet du présent projet sont validés par des expériences de fluorescence et de bioluminescence sur fantômes et animaux vivants.

Salinity and turbidity are two important seawater properties for the physical oceanography. The study of physical oceanography requires a compact high-resolution in-situ salino-turbidi-meter. The main objective of this work is to propose, design and implement an optical solution to simultaneously measure the seawater salinity and turbidity. Our first study is carried out to design a high-resolution refractometer based on a laser beam deviation measurement by a Position Sensitive Device (PSD). The refractive index measurements obtained by the voltage value delivered by PSD have been evaluated to quantify the performances of the sensor. According to the obtained results, it is clear that this PSD-based refractometer is attractive for innovative applications in metrology. However, PSD lacks the capability to retrieve the power distribution information of laser beam, which is related to the turbidity measurements. On the contrary, Charge-Coupled Device (CCD) gives much more information of laser beam than PSD. In the second part of the thesis, a performance comparison between PSD and CCD combined with a centroid algorithm are discussed with special attention paid to the CCD-based refractometer. According to the operating principle of CCD-based system, five factors of CCD-based system: image window size, number of processed images, threshold, binning and saturation are evaluated to optimize the CCD-based refractometer. By applying the optimized parameters, the performance of CCD-based refractometer is better than PSD-based refractometer in measuring the refractive index. Furthermore, by applying different post-processing algorithms, CCD-based system possesses the capability of measuring the power distribution sensitive quantities. To show this advantage of CCD-based system, the attenuation measurement method is used to measure turbidity without modifying the refractometer configuration. The turbidity measurement and salinity measurements influence each other in a refractometer. To overcome these influences, a CCD combined with a new location algorithm is used to measure both the refractive index and the attenuation. Several simulations and experiments are carried out to evaluate this new method. According to the results, the way to improve the resolution is discussed as well. Comparing to the nephelometer specified by the NTU standard, our method has been proved as a valid method to measure turbidity. By studying the performances of CCD-based refracto-turbidi-meter, 3 new prototypes are proposed to improve the salinity and turbidity measurement performance at the end of this thesis.

The BRIght Target Explorer (BRITE) constellation is revolutionary in the sense that the same scientific objectives can be achieved smaller (cm3 versus m3 ) and lighter (< 10kg versus 1, 000kg). It is a space astronomy mission, observing the variations in the apparent brightness of stars. The work presented herein focuses on the assembly, integration and test of the instrument used on-board six nanosatellites that form the constellation. The instrument is composed of an optical telescope equipped with a Charge Coupled Device (CCD) imager and a dedicated computer. This thesis provides a particular in-depth look into the inner workings of CCD. Methods used to characterize the instrument CCD in terms of its bias level stability, gain factor determination, saturation, dark current and readout noise level evaluation are provided. These methodologies are not limited to CCDs and they provide the basis for anyone who wishes to characterize any type of imager for scientic applications.

The BRIght Target Explorer (BRITE) constellation is revolutionary in the sense that the same scientific objectives can be achieved smaller (cm3 versus m3 ) and lighter (< 10kg versus 1, 000kg). It is a space astronomy mission, observing the variations in the apparent brightness of stars. The work presented herein focuses on the assembly, integration and test of the instrument used on-board six nanosatellites that form the constellation. The instrument is composed of an optical telescope equipped with a Charge Coupled Device (CCD) imager and a dedicated computer. This thesis provides a particular in-depth look into the inner workings of CCD. Methods used to characterize the instrument CCD in terms of its bias level stability, gain factor determination, saturation, dark current and readout noise level evaluation are provided. These methodologies are not limited to CCDs and they provide the basis for anyone who wishes to characterize any type of imager for scientic applications.

The main problem addressed by this dissertation is the accurate and automated determination of electron microscope imaging conditions. This enables the restoration of the object wave, which confers direct structural information about the specimen, from sets of differently aberrated images. An important member in the imaging chain is the image recording device, in many cases now a charge-coupled device (CCD) camera. Previous characterisations of these cameras often relied on the unjustified assumption that the Modulation Transfer Function (MTF) also correctly describes the spatial frequency dependent attenuation of the electron shot noise. A new theory is therefore presented that distinguishes between signal and noise transfer. This facilitates the evaluation of both properties using a detailed Monte-Carlo simulation model for the electron and photon scattering in the scintillator of the camera. Furthermore, methods for the accurate experimental determination of the signal and noise transfer functions are presented. In agreement with the Monte-Carlo simulations, experimental results for commercially available CCD cameras show that the signal transfer is significantly poorer than the noise transfer. The centrepiece of this dissertation is the development of new methods for determining the relative aberrations in a set of images and the absolute symmetric aberrations in the restored wave. Both are based on the analysis of the phase information in the Fourier domain and give each Fourier component a weight independent of its strength. This makes the method suitable even for largely crystalline samples with little amorphous contamination, where conventional methods, such as automated diffractogram fitting, usually fail. The method is then extended to also cover the antisymmetric aberrations, using combined beam tilt and focal series. The applicability of the new method is demonstrated with object wave restorations from tilt and focal series of complex inorganic block oxides and of carbon nanotubes filled with one-dimensional inorganic crystals. The latter specimens allowed for the first time a direct comparison between the phase shift in the restored object wave of a specimen with precisely known thickness and the value predicted by simulations.

The main problem addressed by this dissertation is the accurate and automated determination of electron microscope imaging conditions. This enables the restoration of the object wave, which confers direct structural information about the specimen, from sets of differently aberrated images. An important member in the imaging chain is the image recording device, in many cases now a charge-coupled device (CCD) camera. Previous characterisations of these cameras often relied on the unjustified assumption that the Modulation Transfer Function (MTF) also correctly describes the spatial frequency dependent attenuation of the electron shot noise. A new theory is therefore presented that distinguishes between signal and noise transfer. This facilitates the evaluation of both properties using a detailed Monte-Carlo simulation model for the electron and photon scattering in the scintillator of the camera. Furthermore, methods for the accurate experimental determination of the signal and noise transfer functions are presented. In agreement with the Monte-Carlo simulations, experimental results for commercially available CCD cameras show that the signal transfer is significantly poorer than the noise transfer. The centrepiece of this dissertation is the development of new methods for determining the relative aberrations in a set of images and the absolute symmetric aberrations in the restored wave. Both are based on the analysis of the phase information in the Fourier domain and give each Fourier component a weight independent of its strength. This makes the method suitable even for largely crystalline samples with little amorphous contamination, where conventional methods, such as automated diffractogram fitting, usually fail. The method is then extended to also cover the antisymmetric aberrations, using combined beam tilt and focal series. The applicability of the new method is demonstrated with object wave restorations from tilt and focal series of complex inorganic block oxides and of carbon nanotubes filled with one-dimensional inorganic crystals. The latter specimens allowed for the first time a direct comparison between the phase shift in the restored object wave of a specimen with precisely known thickness and the value predicted by simulations.